Protective Effect of Tropical Highland Blackberry Juice (Rubus adenotrichos Schltdl.) Against UVB‐Mediated Damage in Human Epidermal Keratinocytes and in a Reconstituted Skin Equivalent Model
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Solar ultraviolet (UV) radiation, particularly its UVB (280–320 nm) spectrum, is the primary environmental stimulus leading to skin carcinogenesis. Several botanical species with antioxidant properties have shown photochemopreventive effects against UVB damage. Costa Rica's tropical highland blackberry (R ubus adenotrichos) contains important levels of phenolic compounds, mainly ellagitannins and anthocyanins, with strong antioxidant properties. In this study, we examined the photochemopreventive effect of R. adenotrichos blackberry juice (BBJ) on UVB‐mediated responses in human epidermal keratinocytes and in a three‐dimensional (3D) reconstituted normal human skin equivalent (SE). Pretreatment (2 h) and posttreatment (24 h) of normal human epidermal keratinocytes (NHEK s) with BBJ reduced UVB (25 mJ cm−2)‐mediated (1) cyclobutane pyrimidine dimers (CPDs) and (2) 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine (8‐oxodG) formation. Furthermore, treatment of NHEK s with BBJ increased UVB‐mediated (1) poly(ADP‐ribose) polymerase cleavage and (2) activation of caspases 3, 8 and 9. Thus, BBJ seems to alleviate UVB‐induced effects by reducing DNA damage and increasing apoptosis of damaged cells. To establish the in vivo significance of these findings to human skin, immunohistochemistry studies were performed in a 3D SE model, where BBJ was also found to decrease CPD s formation. These data suggest that BBJ may be developed as an agent to ameliorate UV‐induced skin damage.
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The protective effects of natural compounds show a significant impact on skin in various treatment regimens including pre- and post-treatment.
“The protective effects of natural compounds show a significant impact on skin in various treatment regimen including pre- and post-treatment [94].”
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Self-citation
- Authors
- Calvo‐Castro, L., Syed, D. N., Chamcheu, J. C., Vilela, F. M. P., Pérez, A. M., Vaillant, F., Rojas, M., & Mukhtar, H.
- Affiliations
- Instituto Tecnológico de Costa Rica (CR)
- University of Wisconsin–Madison (US)
- Universidade de São Paulo (BR)
- Universidad de Costa Rica (CR)
- Corresponding author
- Mukhtar, Hasan
- ORCID
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 2013-07-08
- Volume
- 89
- Issue
- 5
- Pages
- 1199-1207
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- PMC
- PMC3962679
- OpenAlex
- W1988425414
- Cited by
- 30
- References
- 41
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2020: 4 · 2019: 7 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 1
- FWCI
- 3.12
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Photochemistry and Photobiology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Agritrop (Cirad) (submittedVersion)
- Funders
- National Cancer Institute (P30CA014520)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- Rubus adenotrichos, blackberry juice, UV-B radiation, skin carcinogenesis, human epidermal keratinocytes, cyclobutane pyrimidine dimers, 8-hydroxy-2'-deoxyguanosine, DNA damage, apoptosis, poly(ADP-ribose) polymerase, caspases, ellagitannins, anthocyanins, antioxidant phytochemicals
- MeSH terms
- Cells, Cultured, DNA Damage, Fruit, Humans, Plant Extracts, Radiation-Protective Agents, Skin, Tropical Climate, Ultraviolet Rays, Keratinocytes, Apoptosis, Oxidative Stress
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