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Cited by 50Neuroscience and Neuropharmacology ResearchNeuroendocrine regulation and behaviorCellular transport and secretion

NMDA receptors induce somatodendritic secretion in hypothalamic neurones of lactating female rats

De Kock, Christiaan P. J., Burnashev, Nail, Lodder, Johannes C., Mansvelder, Huibert D., Brussaard, Arjen B.·2004·The Journal of Physiology
DOI 10.1113/jphysiol.2004.069005
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Abstract

Many neurones in the mammalian brain are known to release the content of their vesicles from somatodendritic locations. These vesicles usually contain retrograde messengers that modulate network properties. The back‐propagating action potential is thought to be the principal physiological stimulus that evokes somatodendritic release. In contrast, here we show that calcium influx through NMDA receptor (NMDAR) channels, in the absence of postsynaptic cell firing, is also able to induce vesicle fusion from non‐synaptic sites in nucleated outside‐out patches of dorsomedial supraoptic nucleus (SON) neurones of adult female rats, in particular during their reproductive stages. The physiological significance of this mechanism was characterized in intact brain slices, where NMDAR‐mediated release of oxytocin was shown to retrogradely inhibit presynaptic GABA release, in the absence of postsynaptic cell firing. This implies that glutamatergic synaptic input in itself is sufficient to elicit the release of oxytocin, which in turn acts as a retrograde messenger leading to the depression of nearby GABA synapses. In addition, we found that during lactation, when oxytocin demand is high, NMDA‐induced oxytocin release is up‐regulated compared to that in non‐reproductive rats. Thus, in the hypothalamus, local signalling back and forth between pre‐ and postsynaptic compartments and between different synapses may occur independently of the firing activity of the postsynaptic neurone.

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  • Postsynaptic NMDARs regulate excitability and trigger somatodendritic oxytocin release.

    “Postsynaptic NMDARs also regulate excitability48,49 and trigger somatodendritic oxytocin release47.”

    From Neural circuitry for maternal oxytocin release induced by infant cries (2023) · cites this paper as [47]

  • Oxytocin-neuron NMDARs can be activated without postsynaptic spiking, suggesting that prolonged subthreshold activation of oxytocin cells is sufficient to induce NMDAR-dependent inhibitory LTD.

    “Oxytocin-neuron NMDARs can be activated without the need for postsynaptic spiking47, suggesting that even if PIL inputs are too weak to trigger action potentials, prolonged subthreshold activation of oxytocin cells is sufficient to induce NMDAR-dependent inhibitory LTD.”

    From Neural circuitry for maternal oxytocin release induced by infant cries (2023) · cites this paper as [47]

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Authors
De Kock, C. P. J., Burnashev, N., Lodder, J. C., Mansvelder, H. D., & Brussaard, A. B.
Affiliations
Vrije Universiteit Amsterdam (NL)
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
2004-11
Volume
561
Issue
1
Pages
53-64
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1113/jphysiol.2004.069005
OpenAlex
W2138215802
Cited by
50
References
51
Citations by year
2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 8 · 2013: 3 · 2012: 1
FWCI
0.82
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 22 d)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion)
  • VU Research Portal (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
NMDA receptor, oxytocin release, hypothalamic neurons, lactation, retrograde signaling, GABA release, calcium influx, glutamatergic synaptic input, presynaptic inhibition
MeSH terms
Action Potentials, Animals, Calcium, Dendrites, Female, Hypothalamus, Lactation, Neuronal Plasticity, Neurons, Synapses, Synaptic Vesicles, Signal Transduction, Receptors, N-Methyl-D-Aspartate, Rats, Wistar, Patch-Clamp Techniques, Glutamic Acid, Electric Capacitance, Rats

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