GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis

Goodwin, Jonathan M.; Walkup, Ward G.; Hooper, Kirsty; Li, Taoyingnan; Kishi-Itakura, Chieko; Ng, Aylwin; Lehmberg, Timothy; Jha, Archana; Kommineni, Sravya; Fletcher, Katherine; Garcia-Fortanet, Jorge; Fan, Yaya; Tang, Qing; Wei, Menghao; Agrawal, Asmita; Budhe, Sagar R.; Rouduri, Sreekanth R.; Baird, Dan; Saunders, Jeff; Kiselar, Janna; Chance, Mark R.; Ballabio, Andrea; Appleton, Brent A.; Brumell, John H.; Florey, Oliver; Murphy, Leon O.

doi:10.1126/sciadv.abj2485

Cited by 145

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GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis

Goodwin, Jonathan M., Walkup, Ward G., Hooper, Kirsty, Li, Taoyingnan, Kishi-Itakura, Chieko, Ng, Aylwin, Lehmberg, Timothy, Jha, Archana, Kommineni, Sravya, Fletcher, Katherine, Garcia-Fortanet, Jorge, Fan, Yaya, Tang, Qing, Wei, Menghao, Agrawal, Asmita, Budhe, Sagar R., Rouduri, Sreekanth R., Baird, Dan, Saunders, Jeff, Kiselar, Janna, Chance, Mark R., Ballabio, Andrea, Appleton, Brent A., Brumell, John H., Florey, Oliver, Murphy, Leon O.·2021·Science Advances

DOI 10.1126/sciadv.abj2485PMC PMC10938568

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Abstract

Membrane conjugation of GABARAP sequesters the FLCN-FNIP complex to coordinate activation of MiT/TFE family transcription factors.

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Under certain stress conditions, such as treatment with lysosomal ionophores, lipidation of GABARAP sequesters the FLCN/FNIP complex, inhibiting its GAP activity and resulting in TFEB and TFE3 activation.
“Under certain stress conditions, such as treatment with lysosomal ionophores, lipidation of GABARAP serves as a mechanism to sequester the FLCN/FNIP complex, inhibiting its GAP activity and resulting in TFEB and TFE3 activation30.”
From TMEM55B links autophagy flux, lysosomal repair, and TFE3 activation in response to oxidative stress (2024) · cites this paper as [30]
The Results report that the ionophore monensin induces FLCN-FNIP1 sequestration and FNIP1-LIR-dependent TFEB activation; the Fig. 7 model links GABARAP-dependent sequestration to nuclear retention of TFEB/TFE3. But the Results describe inhibition of FLCN-FNIP GAP activity as a proposed mechanism (“could inhibit”), so the claim states that step more definitively than the source does. No page numbers are available in our copy. (LLM, conf=0.84)
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2 retracted

RETRACTED ARTICLE: Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins
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Background
Retracted
TFEB Links Autophagy to Lysosomal Biogenesis
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The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
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Autophagy-Independent Functions of the Autophagy Machinery
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Background
Transcriptional control of autophagy-lysosome function drives pancreatic cancer metabolism
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LC3-Associated Endocytosis Facilitates β-Amyloid Clearance and Mitigates Neurodegeneration in Murine Alzheimer’s Disease
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The ATG conjugation systems are important for degradation of the inner autophagosomal membrane
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Self-citation
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Sorted by citations

TMEM55B links autophagy flux, lysosomal repair, and TFE3 activation in response to oxidative stress
Jeong, E., Willett, R., Rissone, A., La Spina, M., & Puertollano, R. (2024) · Nature Communications · doi:10.1038/s41467-023-44316-6 · Cited by 25

Authors: Goodwin, J. M., Walkup, W. G., Hooper, K., Li, T., Kishi-Itakura, C., Ng, A., Lehmberg, T., Jha, A., Kommineni, S., Fletcher, K., Garcia-Fortanet, J., Fan, Y., Tang, Q., Wei, M., Agrawal, A., Budhe, S. R., Rouduri, S. R., Baird, D., Saunders, J., Kiselar, J., Chance, M. R., Ballabio, A., Appleton, B. A., Brumell, J. H., Florey, O., & Murphy, L. O.
Venue: Science Advances
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2021-10
Volume: 7
Issue: 40
ISSN: 2375-2548
DOI: 10.1126/sciadv.abj2485
PMC: PMC10938568
Cited by: 145
References: 15
Language: en

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GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis

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