Cited by 2,138Fungal and yeast genetics researchBioinformatics and Genomic NetworksGene Regulatory Network Analysis

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants

Tong, Amy Hin Yan, Evangelista, Marie, Parsons, Ainslie B., Xu, Hong, Bader, Gary D., Pagé, Nicholas, Robinson, Mark, Raghibizadeh, Sasan, Hogue, Christopher W. V., Bussey, Howard, Andrews, Brenda, Tyers, Mike, Boone, Charles·2001·Science

DOI 10.1126/science.1065810

Open this paper in CoChat — free account

Abstract

In Saccharomyces cerevisiae , more than 80% of the ∼6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization ( BNI1 , ARP2 , ARC40 , BIM1 ), DNA synthesis and repair ( SGS1 , RAD27 ), or uncharacterized functions ( BBC1 , NBP2 ) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

Integrity signals

Registry

Crossref · 10.1126/science.1065810

Cites

23 of 23 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis
Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., et al. (1999) · Science · doi:10.1126/science.285.5429.901 · Cited by 3,953
Self-citation
BIND--The Biomolecular Interaction Network Database
Bader, G. D. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.1.242 · Cited by 1,560
Self-citation
Principles for the buffering of genetic variation
Hartman, J. L., Garvik, B., & Hartwell, L. (2001) · Science · doi:10.1126/science.1056072 · Cited by 808
Integrating Genetic Approaches into the Discovery of Anticancer Drugs
Hartwell, L. H., Szankasi, P., Roberts, C. J., Murray, A. W., & Friend, S. H. (1997) · Science · doi:10.1126/science.278.5340.1064 · Cited by 789
Bni1p, a Yeast Formin Linking Cdc42p and the Actin Cytoskeleton During Polarized Morphogenesis
Evangelista, M., Blundell, K., Longtine, M. S., Chow, C. J., Adames, N. R., Pringle, J. R., Peter, M., & Boone, C. M. (1997) · Science · doi:10.1126/science.276.5309.118 · Cited by 647
Self-citation
Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar–coding strategy
Shoemaker, D. D., Lashkari, D. A., Morris, D., Mittmann, M., & Davis, R. W. (1996) · Nature Genetics · doi:10.1038/ng1296-450 · Cited by 569
Widespread aneuploidy revealed by DNA microarray expression profiling
Hughes, T. R., Roberts, C. J., Dai, H. A., Jones, A. R., Meyer, M. R., Slade, D., Burchard, J., Dow, S., Ward, T. R., Kidd, M. J., et al. (2000) · Nature Genetics · doi:10.1038/77116 · Cited by 482
Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.
Bender, A., & Pringle, J. R. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.3.1295 · Cited by 433
Robustness against mutations in genetic networks of yeast
Wagner, A. (2000) · Nature Genetics · doi:10.1038/74174 · Cited by 411
Requirement for Three Novel Protein Complexes in the Absence of the Sgs1 DNA Helicase in Saccharomyces cerevisiae
Mullen, J. R., Kaliraman, V., Ibrahim, S. S., & Brill, S. J. (2001) · Genetics · doi:10.1093/genetics/157.1.103 · Cited by 405

Flagged first, then by citations

Show all 23 references

Research works printed but not linked: 4

Pringle J. R., Mol. Cell. Biol. 11, 1295 (1991).
I. Herskowitz J. Rine J. Strathern in The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae vol. 2 Gene Expression E. W. Jones J. R. Pringle J. R. Broach Eds. (Cold Spring Harbor Laboratory Cold Spring Harbor NY 1992) pp. 583–656.
M. Evangelista et al. Nature Cell Biol. in press.
The following supplementary material is available on Science Online at www.sciencemag.org/cgi/content/full/294/5550/2364/DC1: supplementary table 1 a table of the deletion mutants used to create the double-mutant arrays shown in Fig. 2A by SGA analysis; supplementary table 2 a table of the 4672 unique deletion mutants assembled for large-scale SGA analysis; supplementary notes a description of the materials and methods for SGA analysis and bioinformatic analysis (yeast strains will be provided upon request); supplementary table 3 a table summarizing all the synthetic lethal/fitness interactions; supplementary table 4 a table of 523 gene deletions within the array that often appeared to be synthetically lethal with a wild-type control strain; supplementary table 5 a table of the overlap of the genetic interactions presented in Fig. 3 and the published genetic interactions assembled at MIPS (Munich Information Center for Protein Sequences) and YPD; supplementary fig. 1 a figure of the protein-protein interactions corresponding to genes within the genetic-interaction network shown in Fig. 3; supplementary table 6 a table of the gene deletions that are linked to the query gene and appeared to be synthetically lethal; supplementary fig. 2 a figure that shows two-dimensional clustering analysis of synthetic lethal interactions; supplementary table 7 a table that shows an analysis of the false-positives for the BIM1 and SGS1 screens as a comparison of the list of gene deletions that were scored as positive by SGA analysis to the list of gene deletions that were ultimately confirmed to be synthetically lethal by tetrad analysis; and supplementary table 8 a table of the synthetic genetic interactions and their BIND identification numbers.

Sorted by citations

Items 501–600 of 1,004

Disruption of Mechanisms That Prevent Rereplication Triggers a DNA Damage Response
Archambault, V., Ikui, A. E., Drapkin, B. J., & Cross, F. R. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.15.6707-6721.2005 · Cited by 75
Modelling genotype–phenotype relationships and human disease with genetic interaction networks
Lehner, B. (2007) · Journal of Experimental Biology · doi:10.1242/jeb.002311 · Cited by 76
Human ribosomal protein L13a is dispensable for canonical ribosome function but indispensable for efficient rRNA methylation
Chaudhuri, S., Vyas, K., Kapasi, P., Komar, A. A., Dinman, J. D., Barik, S., & Mazumder, B. (2007) · RNA · doi:10.1261/rna.694007 · Cited by 75
Nucleotide degradation and ribose salvage in yeast
Xu, Y., Létisse, F., Absalan, F., Lu, W., Kuznetsova, E., Brown, G., Caudy, A. A., Yakunin, A. F., Broach, J. R., & Rabinowitz, J. D. (2013) · Molecular Systems Biology · doi:10.1038/msb.2013.21 · Cited by 77
The Yeast AAA+ Chaperone Hsp104 Is Part of a Network That Links the Actin Cytoskeleton with the Inheritance of Damaged Proteins
Tessarz, P., Schwarz, M., Mogk, A., & Bukau, B. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.00201-09 · Cited by 78
Tailoring the genome: the power of genetic approaches
Nagy, A., Perrimon, N., Sandmeyer, S. B., & Plasterk, R. H. A. (2003) · Nature Genetics · doi:10.1038/ng1115 · Cited by 75
Loss-of-function genetic screens as a tool to improve the diagnosis and treatment of cancer
Mullenders, J., & Bernards, R. (2009) · Oncogene · doi:10.1038/onc.2009.295 · Cited by 76
Synthetic Lethality of Cohesins with PARPs and Replication Fork Mediators
McLellan, J. L., O’Neil, N. J., Barrett, I. J., Ferree, E. J., van Pel, D. M., Ushey, K., Sipahimalani, P., Bryan, J., Rose, A. M., & Hieter, P. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002574 · Cited by 77
Computational systems biology of the cell cycle
Csikász‐Nagy, A. (2009) · Briefings in Bioinformatics · doi:10.1093/bib/bbp005 · Cited by 76
A Screen for Genes of Heme Uptake Identifies the FLC Family Required for Import of FAD into the Endoplasmic Reticulum
Protchenko, O. V., Rodriguez-Suarez, R. J., Androphy, R., Bussey, H., & Philpott, C. C. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m512812200 · Cited by 74
Self-citation
Evolving Therapeutic Strategies to Exploit Chromosome Instability in Cancer
Thompson, L., Jeusset, L. M., Lepage, C. C., & McManus, K. J. (2017) · Cancers · doi:10.3390/cancers9110151 · Cited by 80
Toward an integrated map of genetic interactions in cancer cells
Rauscher, B., Heigwer, F., Henkel, L., Hielscher, T., Voloshanenko, O., & Boutros, M. (2018) · Molecular Systems Biology · doi:10.15252/msb.20177656 · Cited by 77
Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for Microorganisms
van Opijnen, T., Lazinski, D. W., & Camilli, A. (2015) · Current Protocols in Microbiology · doi:10.1002/9780471729259.mc01e03s36 · Cited by 74
Evolution of the yeast protein interaction network
Qin, H. H., Lu, H. H., Wu, W. B., & Li, W. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2235584100 · Cited by 73
Experimental evolution of a sexually selected display in yeast
Rogers, D. W., & Greig, D. (2008) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.2008.1146 · Cited by 72
Environmental robustness of the global yeast genetic interaction network
Costanzo, M., Hou, J., Messier, V., Nelson, J., Rahman, M., VanderSluis, B., Wang, W., Pons, C., Ross, C., Ušaj, M., et al. (2021) · Science · doi:10.1126/science.abf8424 · Cited by 75
Self-citation
Quantitative Fitness Analysis Shows That NMD Proteins and Many Other Protein Complexes Suppress or Enhance Distinct Telomere Cap Defects
Addinall, S. G., Holstein, E., Lawless, C., Yu, M., Chapman, K. E., Banks, A. P., Ngo, H.-P., Maringele, L., Taschuk, M. L., Young, A. E., et al. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1001362 · Cited by 72
Chemical-genetic profile analysis of five inhibitory compounds in yeast
Alamgir, M. S., Erukova, V., Jessulat, M., Azizi, A., & Golshani, A. (2010) · BMC Chemical Biology · doi:10.1186/1472-6769-10-6 · Cited by 72
Rewiring of Transcriptional Regulatory Networks: Hierarchy, Rather Than Connectivity, Better Reflects the Importance of Regulators
Bhardwaj, N., Kim, P. M., & Gerstein, M. (2010) · Science Signaling · doi:10.1126/scisignal.2001014 · Cited by 74
Histone H3 K56 Hyperacetylation Perturbs Replisomes and Causes DNA Damage
Celic, I., Verreault, A., & Boeke, J. D. (2008) · Genetics · doi:10.1534/genetics.108.088914 · Cited by 71
The Arabidopsis AtSTE24 Is a CAAXProtease with Broad Substrate Specificity
Bracha‐Drori, K., Lavy, M., & Yalovsky, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202916200 · Cited by 71
Protein-protein Interaction Networks: from Interactions to Networks
Cho, S., Park, S.-G., Lee, D. H., & Park, B.-C. (2004) · BMB Reports · doi:10.5483/bmbrep.2004.37.1.045 · Cited by 71
Cellular Processes and Pathways That Protect Saccharomyces cerevisiae Cells against the Plasma Membrane-Perturbing Compound Chitosan
Zakrzewska, A., Boorsma, A., Delneri, D., Brul, S., Oliver, S. G., & Klis, F. M. (2007) · Eukaryotic Cell · doi:10.1128/ec.00355-06 · Cited by 71
Yeast9: a consensus genome-scale metabolic model for S. cerevisiae curated by the community
Zhang, C., Sánchez, B. J., Li, F., Eiden, C. W. Q., Scott, W. T., Liebal, U. W., Blank, L. M., Mengers, H. G., Anton, M., Rangel, A. E. T., et al. (2024) · Molecular Systems Biology · doi:10.1038/s44320-024-00060-7 · Cited by 83
MYST opportunities for growth control: yeast genes illuminate human cancer gene functions
Lafon, A., Chang, C., Scott, E. M., Jacobson, S. J., & Pillus, L. (2007) · Oncogene · doi:10.1038/sj.onc.1210606 · Cited by 72
The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism
Parnell, T. J., Schlichter, A., Wilson, B. G., & Cairns, B. R. (2015) · eLife · doi:10.7554/elife.06073 · Cited by 70
Assays to Monitor Autophagy in Saccharomyces cerevisiae
Torggler, R., Papinski, D., & Kraft, C. (2017) · Cells · doi:10.3390/cells6030023 · Cited by 78
She4p/Dim1p Interacts with the Motor Domain of Unconventional Myosins in the Budding Yeast,Saccharomyces cerevisiae
Toi, H., Fujimura‐Kamada, K., Irie, K., Takai, Y., Todo, S., & Tanaka, K. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-09-0616 · Cited by 70
Super models
Barr, M. M. (2003) · Physiological Genomics · doi:10.1152/physiolgenomics.00075.2002 · Cited by 70
Functional wiring of the yeast kinome revealed by global analysis of genetic network motifsPreprint
Sharifpoor, S., van Dyk, D., Costanzo, M., Baryshnikova, A., Friesen, H., Douglas, A. C., Youn, J.-Y., VanderSluis, B., Myers, C. L., Papp, B., et al. (2012) · Genome Research · doi:10.1101/gr.129213.111 · Cited by 71
Self-citation
Reverse engineering gene regulatory networks
Huang, Y., Tienda-Luna, I. M., & Wang, Y. (2009) · IEEE Signal Processing Magazine · doi:10.1109/msp.2008.930647 · Cited by 69
The emergence of lipid droplets in yeast: current status and experimental approaches
Radulovic, M., Knittelfelder, O. L., Cristobal-Sarramian, A., Kolb, D., Wolinski, H., & Kohlwein, S. D. (2013) · Current Genetics · doi:10.1007/s00294-013-0407-9 · Cited by 69
Mechanism and cellular function of Bud6 as an actin nucleation–promoting factor
Graziano, B. R., DuPage, A. G., Michelot, A., Breitsprecher, D., Moseley, J. B., Sagot, I., Blanchoin, L., & Goode, B. L. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-05-0404 · Cited by 69
Broad metabolic sensitivity profiling of a prototrophic yeast deletion collection
VanderSluis, B., Hess, D. C., Pesyna, C., Krumholz, E. W., Syed, T., Szappanos, B., Nislow, C., Papp, B., Troyanskaya, O. G., Myers, C. L., et al. (2014) · Genome biology · doi:10.1186/gb-2014-15-4-r64 · Cited by 69
A robust platform for chemical genomics in bacterial systems
French, S., Mangat, C. S., Bharat, A., Côté, J., Mori, H., & Brown, E. D. (2016) · Molecular Biology of the Cell · doi:10.1091/mbc.e15-08-0573 · Cited by 69
dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae
Pan, X., Yuan, D., Ooi, S.-L., Wang, X., Sookhai-Mahadeo, S., Meluh, P. B., & Boeke, J. D. (2006) · Methods · doi:10.1016/j.ymeth.2006.07.033 · Cited by 69
The Hog1 Mitogen-Activated Protein Kinase Mediates a Hypoxic Response in Saccharomyces cerevisiae
Hickman, M. J., Spatt, D., & Winston, F. (2011) · Genetics · doi:10.1534/genetics.111.128322 · Cited by 68
Protein-protein interactions: Methods, databases, and applications in virus-host study
Farooq, Q. U. A., Shaukat, Z., Aiman, S., & Li, C. (2021) · World Journal of Virology · doi:10.5501/wjv.v10.i6.288 · Cited by 71
Regulation of histone gene transcription in yeast
Kurat, C. F., Recht, J., Radovani, E., Durbic, T., Andrews, B., & Fillingham, J. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1443-9 · Cited by 70
Self-citation
Asymmetric Inheritance of Aggregated Proteins and Age Reset in Yeast Are Regulated by Vac17-Dependent Vacuolar Functions
Hill, S. M., Hao, X., Grönvall, J., Spikings-Nordby, S., Widlund, P. O., Amen, T., Jörhov, A., Josefson, R., Kaganovich, D., Liu, B., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.06.016 · Cited by 70
Rex1p deficiency leads to accumulation of precursor initiator tRNAMet and polyadenylation of substrate RNAs in Saccharomyces cerevisiae
Ozanick, S. G., Wang, X., Costanzo, M., Brost, R. L., Boone, C. M., & Anderson, J. T. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn925 · Cited by 68
Self-citation
MRC1-dependent scaling of the budding yeast DNA replication timing programPreprint
Koren, A., Soifer, I., & Barkai, N. (2010) · Genome Research · doi:10.1101/gr.102764.109 · Cited by 68
Preservation of genetic and regulatory robustness in ancient gene duplicates of Saccharomyces cerevisiaePreprint
Keane, O. M., Toft, C., Carretero‐Paulet, L., Jones, G. W., & Fares, M. A. (2014) · Genome Research · doi:10.1101/gr.176792.114 · Cited by 69
Genetic and Biochemical Analysis of Yeast and Human Cap Trimethylguanosine Synthase
Hausmann, S., Zheng, S., Costanzo, M., Brost, R. L., Garcin, D., Boone, C. M., Shuman, S., & Schwer, B. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m806127200 · Cited by 67
Self-citation
Sequential and Distinct Roles of the Cadherin Domain-containing Protein Axl2p in Cell Polarization in Yeast Cell Cycle
Gao, X., Sperber, L. M., Kane, S. A., Tong, Z., Tong, A. H. Y., Boone, C. M., & Bi, E. (2007) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-09-0822 · Cited by 67
Self-citation
Biological network analysis: insights into structure and functions
Ma, X., & Gao, L. (2012) · Briefings in Functional Genomics · doi:10.1093/bfgp/els045 · Cited by 67
Effect of training datasets on support vector machine prediction of protein‐protein interactions
Lo, S. L., Cai, C., Chen, Y., & Chung, M. C. M. (2005) · PROTEOMICS · doi:10.1002/pmic.200401118 · Cited by 67
A pathway of targeted autophagy is induced by DNA damage in budding yeast
Eapen, V. V., Waterman, D. P., Bernard, A., Schiffmann, N., Sayas, E., Kamber, R., Lemos, B., Memisoglu, G., Ang, J., Mazella, A., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1614364114 · Cited by 62
Using Large Datasets to Understand Nanotechnology
Paunovska, K., Loughrey, D., Sago, C. D., Langer, R., & Dahlman, J. E. (2019) · Advanced Materials · doi:10.1002/adma.201902798 · Cited by 69
A Screen for Suppressors of Gross Chromosomal Rearrangements Identifies a Conserved Role for PLP in Preventing DNA Lesions
Kanellis, P., Gagliardi, M., Banáth, J. P., Szilard, R. K., Nakada, S., Galicia, S., Sweeney, F. D., Cabelof, D. C., Olive, P. L., & Durocher, D. (2007) · PLoS Genetics · doi:10.1371/journal.pgen.0030134 · Cited by 69
Cyclophilin A Is Localized to the Nucleus and Controls Meiosis in Saccharomyces cerevisiae
Arévalo-Rodrı́guez, M., & Heitman, J. (2005) · Eukaryotic Cell · doi:10.1128/ec.4.1.17-29.2005 · Cited by 67
Local chromatin structure at the ribosomal DNA causes replication fork pausing and genome instability in the absence of the S. cerevisiae DNA helicase Rrm3pPreprint
Torres, J. Z., Bessler, J. B., & Zakian, V. A. (2004) · Genes & Development · doi:10.1101/gad.1154704 · Cited by 67
The Rtt106 Histone Chaperone Is Functionally Linked to Transcription Elongation and Is Involved in the Regulation of Spurious Transcription from Cryptic Promoters in Yeast*♦
Imbeault, D., Gamar, L., Rufiange, A., Paquet, E., & Nourani, A. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.c800147200 · Cited by 66
Microtubule-Stabilizing Drugs from Marine Sponges: Focus on Peloruside A and Zampanolide
Miller, J. H., Singh, A. J., & Northcote, P. T. (2010) · Marine Drugs · doi:10.3390/md8041059 · Cited by 67
The function, structure, and origins of the ER membrane protein complex
Hegde, R. S. (2022) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-032620-104553 · Cited by 61
Chaperone-mediated reflux of secretory proteins to the cytosol during endoplasmic reticulum stress
Igbaria, A., Merksamer, P. I., Trusina, A., Tilahun, F., Johnson, J. R., Brandman, O., Krogan, N. J., Weissman, J. S., & Papa, F. R. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1904516116 · Cited by 68
Barcoded bulk QTL mapping reveals highly polygenic and epistatic architecture of complex traits in yeast
Ba, A. N. N., Lawrence, K. R., Rego‐Costa, A., Gopalakrishnan, S., Temko, D., Michor, F., & Desai, M. M. (2022) · eLife · doi:10.7554/elife.73983 · Cited by 68
Caenorhabditis elegans EFA-6 limits microtubule growth at the cell cortex
O'Rourke, S. M., Christensen, S. N., & Bowerman, B. A. (2010) · Nature Cell Biology · doi:10.1038/ncb2128 · Cited by 67
Microbial sensor cell arrays
Melamed, S., Elad, T., & Belkin, S. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.11.024 · Cited by 65
The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus
Aguilera-Romero, A., Kamińska, J., Spang, A., Riezman, H., & Muñiz, M. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200710025 · Cited by 65
Colony size measurement of the yeast gene deletion strains for functional genomics
Memarian, N., Jessulat, M., Alirezaie, J., Mir-Rashed, N., Xu, J., Zareie, M., Smith, M. L., & Golshani, A. (2007) · BMC Bioinformatics · doi:10.1186/1471-2105-8-117 · Cited by 65
The human Bloom syndrome gene suppresses the DNA replication and repair defects of yeast dna2 mutants
Imamura, O., & Campbell, J. L. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1431624100 · Cited by 65
Rad62 Protein Functionally and Physically Associates with the Smc5/Smc6 Protein Complex and Is Required for Chromosome Integrity and Recombination Repair in Fission Yeast
Morikawa, H., Morishita, T., Kawane, S., Iwasaki, H., Carr, A. M., & Shinagawa, H. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.21.9401-9413.2004 · Cited by 65
Synthetic Quantitative Array Technology Identifies the Ubp3-Bre5 Deubiquitinase Complex as a Negative Regulator of Mitophagy
Müller, M., Kötter, P., Behrendt, C., Walter, E., Scheckhuber, C. Q., Entian, K.-D., & Reichert, A. S. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.01.044 · Cited by 67
Lagging Strand Replication Proteins in Genome Stability and DNA Repair
Rossi, M. L., Purohit, V., Brandt, P. D., & Bambara, R. A. (2005) · Chemical Reviews · doi:10.1021/cr040497l · Cited by 65
Greedily building protein networks with confidence
Bader, J. S. (2003) · Bioinformatics · doi:10.1093/bioinformatics/btg358 · Cited by 64
Human eIF4AIII interacts with an eIF4G-like partner, NOM1, revealing an evolutionarily conserved function outside the exon junction complexPreprint
Alexandrov, A., Colognori, D., & STEITZ, J. A. (2011) · Genes & Development · doi:10.1101/gad.2045411 · Cited by 64
Scope and limitations of yeast as a model organism for studying human tissue-specific pathways
Mohammadi, S., Saberidokht, B., Subramaniam, S., & Grama, A. Y. (2015) · BMC Systems Biology · doi:10.1186/s12918-015-0253-0 · Cited by 74
The Prefoldin Complex Regulates Chromatin Dynamics during Transcription Elongation
Millán-Zambrano, G., Rodríguez‐Gil, A., Peñate, X., de Miguel-Jiménez, L., Morillo‐Huesca, M., Krogan, N. J., & Chávez, S. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003776 · Cited by 64
Predicting genetic interactions with random walks on biological networks
Chipman, K. C., & Singh, A. K. (2009) · BMC Bioinformatics · doi:10.1186/1471-2105-10-17 · Cited by 63
Genome-Wide Analysis of Sterol-Lipid Storage and Trafficking in Saccharomyces cerevisiae
Fei, W., Alfaro, G., Muthusamy, B.-P., Klaassen, Z. W. A., Graham, T. R., Yang, H., & Beh, C. T. (2007) · Eukaryotic Cell · doi:10.1128/ec.00386-07 · Cited by 63
The RSC Chromatin Remodeling Complex Bears an Essential Fungal-Specific Protein Module With Broad Functional Roles
Wilson, B. G., Erdjument‐Bromage, H., Tempst, P., & Cairns, B. R. (2005) · Genetics · doi:10.1534/genetics.105.047589 · Cited by 63
PathSys: integrating molecular interaction graphs for systems biology
Baitaluk, M., Qian, X., Godbole, S., Raval, A., Ray, A., & Gupta, A. (2006) · BMC Bioinformatics · doi:10.1186/1471-2105-7-55 · Cited by 63
Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence
Azam, M. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkj452 · Cited by 63
Cu, Zn Superoxide Dismutase and NADP(H) Homeostasis Are Required for Tolerance of Endoplasmic Reticulum Stress inSaccharomyces cerevisiae
Tan, S., Teo, M., Lam, Y. T., Dawes, I. W., & Perrone, G. G. (2009) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-07-0697 · Cited by 62
Bioinformatics in proteomics
Vihinen, M. (2001) · Biomolecular Engineering · doi:10.1016/s1389-0344(01)00099-5 · Cited by 62
Yeast Tdh3 (Glyceraldehyde 3-Phosphate Dehydrogenase) Is a Sir2-Interacting Factor That Regulates Transcriptional Silencing and rDNA Recombination
Ringel, A. E., Ryznar, R. J., Picariello, H. S., Huang, K., Lazarus, A., & Holmes, S. G. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003871 · Cited by 66
Antibiotics as probes of biological complexity
Falconer, S. B., Czarny, T. L., & Brown, E. D. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.590 · Cited by 67
Systematic Genome Reductions: Theoretical and Experimental Approaches
Fehér, T., Papp, B., Pál, C., & Pósfai, G. (2007) · Chemical Reviews · doi:10.1021/cr0683111 · Cited by 62
Functional Characterization of Dma1 and Dma2, the Budding Yeast Homologues ofSchizosaccharomyces pombeDma1 and Human Chfr
Fraschini, R., Bilotta, D., Lucchini, G., & Piatti, S. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-02-0094 · Cited by 62
The coordinated evolution of yeast proteins is constrained by functional modularity
Chen, Y., & Dokholyan, N. V. (2006) · Trends in Genetics · doi:10.1016/j.tig.2006.06.008 · Cited by 62
Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii
Dalvie, N. C., Leal, J., Whittaker, C. A., Yang, Y., Brady, J. R., Love, K. R., & Love, J. C. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00372 · Cited by 62
GenePath: a system for automated construction of genetic networks from mutant data
Zupan, B., Demšar, J., Ivan Bratko, A., Juvan, P., Halter, J. A., Kuspa, A., & Shaulsky, G. (2003) · Bioinformatics · doi:10.1093/bioinformatics/btf871 · Cited by 62
Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptakePreprint
Johansson, M., & Jacobson, A. (2010) · Genes & Development · doi:10.1101/gad.1930710 · Cited by 62
Advances in fungal proteomics
Bhadauria, V., Zhao, W., Wang, L., Zhang, Y., Liu, J., Yang, J., Kong, L., & Peng, Y. (2007) · Microbiological Research · doi:10.1016/j.micres.2007.03.001 · Cited by 61
Balony:a software package for analysis of data generated by synthetic genetic array experiments
Young, B. P., & Loewen, C. J. R. (2013) · BMC Bioinformatics · doi:10.1186/1471-2105-14-354 · Cited by 61
Wss1 Is a SUMO-Dependent Isopeptidase That Interacts Genetically with the Slx5-Slx8 SUMO-Targeted Ubiquitin Ligase
Mullen, J. R., Chen, C.-F., & Brill, S. J. (2010) · Molecular and Cellular Biology · doi:10.1128/mcb.01649-09 · Cited by 61
Suppression of α-synuclein toxicity and vesicle trafficking defects by phosphorylation at S129 in yeast depends on genetic context
Sancenón, V., Lee, S.-A., Patrick, C., Griffith, J. M., Paulino, A., Outeiro, T. F., Reggiori, F., Masliah, E., & Muchowski, P. J. (2012) · Human Molecular Genetics · doi:10.1093/hmg/dds058 · Cited by 63
Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase
Wood, C. S., Hung, C.-S., Huoh, Y., Mousley, C. J., Stefan, C. J., Bankaitis, V. A., Ferguson, K. M., & Burd, C. G. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e12-01-0077 · Cited by 61
A genome‐scale yeast library with inducible expression of individual genes
Arita, Y., Kim, G., Li, Z., Friesen, H., Turco, G. M., Wang, R. Y., Climie, D., Ušaj, M., Hotz, M., Stoops, E. H., et al. (2021) · Molecular Systems Biology · doi:10.15252/msb.202110207 · Cited by 61
Self-citation
Role of the Septin Ring in the Asymmetric Localization of Proteins at the Mother-Bud Neck inSaccharomyces cerevisiae
Kozubowski, L., Larson, J. R., & Tatchell, K. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-09-0764 · Cited by 61
Emerging trends in high-throughput screening
Entzeroth, M. (2003) · Current Opinion in Pharmacology · doi:10.1016/s1471-4892(03)00088-2 · Cited by 61
Bioinformatic analysis of autism positional candidate genes using biological databases and computational gene network prediction
Yonan, A. L., Palmer, A. A., Smith, K. C., Feldman, I., Lee, H. K., Yonan, J., Fischer, S. G., Pavlidis, P., & Gilliam, T. C. (2003) · Genes Brain & Behavior · doi:10.1034/j.1601-183x.2003.00041.x · Cited by 61
High-throughput functional characterization of protein phosphorylation sites in yeast
Viéitez, C., Busby, B. P., Ochoa, D., Mateus, A., Memon, D., Galardini, M., Yildiz, U., Trovato, M., Jawed, A., Geiger, A. G., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01051-x · Cited by 60
RNA Polymerase II Carboxyl-terminal Domain Phosphorylation Regulates Protein Stability of the Set2 Methyltransferase and Histone H3 Di- and Trimethylation at Lysine 36
Fuchs, S. M., Kizer, K. O., Braberg, H., Krogan, N. J., & Strahl, B. D. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.273953 · Cited by 64
Genome-wide Screen Identifies Pathways that Govern GAA/TTC Repeat Fragility and Expansions in Dividing and Nondividing Yeast Cells
Zhang, Y., Shishkin, A. A., Nishida, Y., Marcinkowski-Desmond, D., Saini, N., Volkov, K. V., Mirkin, S. M., & Lobachev, K. S. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.08.002 · Cited by 60
Regulation of Rtt107 Recruitment to Stalled DNA Replication Forks by the Cullin Rtt101 and the Rtt109 Acetyltransferase
Roberts, T. M., Zaidi, I. W., Vaisica, J. A., Peter, M., & Brown, G. W. (2007) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-09-0961 · Cited by 61
The RNA Polymerase II Kinase Ctk1 Regulates Positioning of a 5′ Histone Methylation Boundary along Genes
Xiao, T., Shibata, Y., Rao, B., Laribee, R. N., O'Rourke, R., Buck, M. J., Greenblatt, J., Krogan, N. J., Lieb, J. D., & Strahl, B. D. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.01628-06 · Cited by 60
Lethal combinations
Tucker, C. L., & Fields, S. (2003) · Nature Genetics · doi:10.1038/ng1103-204 · Cited by 60
Control of Translocations between Highly Diverged Genes by Sgs1, the Saccharomyces cerevisiae Homolog of the Bloom'sSyndrome Protein
Schmidt, K. H., Wu, J., & Kolodner, R. D. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.00161-06 · Cited by 60

Authors: Tong, A. H. Y., Evangelista, M., Parsons, A. B., Xu, H., Bader, G. D., Pagé, N., Robinson, M., Raghibizadeh, S., Hogue, C. W. V., Bussey, H., Andrews, B., Tyers, M., & Boone, C.
Affiliations: University of Toronto (CA)
Queen's University (CA)
Mount Sinai Hospital (CA)
McGill University (CA)
Virtek Engineering Sciences, Inc. (VESI), 1 Bedford Road, Toronto ON, Canada M5R 2J7.
Corresponding author: Andrews, Brenda
Tyers, Mike
Boone, Charles
ORCID: Tong, Amy Hin Yan
Evangelista, Marie
Xu, Hong
Bader, Gary D.
Robinson, Mark
Hogue, Christopher W. V.
Andrews, Brenda
Tyers, Mike
Boone, Charles
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2001-12-14
Volume: 294
Issue: 5550
Pages: 2364-2368
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1065810
OpenAlex: W2127073167
Cited by: 2,138
References: 23
Citations by year: 2026: 24 · 2025: 39 · 2024: 51 · 2023: 51 · 2022: 49 · 2021: 67 · 2020: 71 · 2019: 63 · 2018: 65 · 2017: 105 · 2016: 73 · 2015: 96 · 2014: 115 · 2013: 101 · 2012: 107
FWCI: 27.25
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords: synthetic genetic array, yeast deletion mutants, genetic interactions, cytoskeletal organization, non-essential genes, Saccharomyces cerevisiae
MeSH terms: Carrier Proteins, Crosses, Genetic, Cytoskeletal Proteins, Cytoskeleton, DNA Repair, DNA Helicases, DNA, Fungal, Endodeoxyribonucleases, Fungal Proteins, Genes, Fungal, Genetic Markers, Genetic Techniques, Microfilament Proteins, Microtubule Proteins, Mitosis, Recombination, Genetic, Robotics, Saccharomyces cerevisiae, Genome, Fungal, Cell Polarity, Gene Deletion, Cell Cycle Proteins, Computational Biology, Genes, Essential, Saccharomyces cerevisiae Proteins, Databases, Genetic, Flap Endonucleases, RecQ Helicases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks