Cited by 6,382Cellular Mechanics and InteractionsForce Microscopy Techniques and ApplicationsHydrogels: synthesis, properties, applications

The DOI matches its Crossref record; 82 of the 82 references it cites were found in a registry or an index.

Tissue Cells Feel and Respond to the Stiffness of Their Substrate

Discher, Dennis E., Janmey, Paul, Wang, Yu-li·2005·Science

DOI 10.1126/science.1116995

Open this paper in CoChat — free account

Abstract

Normal tissue cells are generally not viable when suspended in a fluid and are therefore said to be anchorage dependent. Such cells must adhere to a solid, but a solid can be as rigid as glass or softer than a baby's skin. The behavior of some cells on soft materials is characteristic of important phenotypes; for example, cell growth on soft agar gels is used to identify cancer cells. However, an understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels (or to other cells) with which elasticity can be tuned to approximate that of tissues. Key roles in molecular pathways are played by adhesion complexes and the actinmyosin cytoskeleton, whose contractile forces are transmitted through transcellular structures. The feedback of local matrix stiffness on cell state likely has important implications for development, differentiation, disease, and regeneration.

Integrity signals

Registry

Crossref · 10.1126/science.1116995

Cites

82 of 82 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

New Perspectives in Cell Adhesion: RGD and Integrins
Ruoslahti, E. I., & Pierschbacher, M. D. (1987) · Science · doi:10.1126/science.2821619 · Cited by 4,875
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Self-citation
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,082
Self-citation
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Exploring and Engineering the Cell Surface Interface
Stevens, M. M., & George, J. H. (2005) · Science · doi:10.1126/science.1106587 · Cited by 2,607
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Self-citation
Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
Balaban, N. Q., Schwarz, U. S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., Mahalu, D., Safran, S. A., Bershadsky, A. D., Addadi, L., et al. (2001) · Nature Cell Biology · doi:10.1038/35074532 · Cited by 2,137
Cells lying on a bed of microneedles: An approach to isolate mechanical force
Tan, J. L., Tien, J., Pirone, D. M., Gray, D. S., Bhadriraju, K., & Chen, C. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0235407100 · Cited by 2,002
Myotubes differentiate optimally on substrates with tissue-like stiffness
Engler, A. J., Griffin, M. A., Sen, S., Bönnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200405004 · Cited by 1,743
Self-citation
Nonlinear elasticity in biological gels
Storm, C., Pastore, J. J., MacKintosh, F. C., Lubensky, T. C., & Janmey, P. A. (2005) · Nature · doi:10.1038/nature03521 · Cited by 1,707
Self-citation

Flagged first, then by citations

Show all 82 references

Research works printed but not linked: 5

D. R. Welch et al., Cancer Res.60, 1552 (2000).
F. J. Alenghat, D. E. Ingber, Sci. STKE119, pe6 (2002).
A. Saez A. Buguin P. Silberzan B. Ladoux Biophys J. in press.
J. P. Trinkaus, J. P. Lentz, Dev. Biol.89, 115 (1964).
W. T. Arthur, N. K. Noren, K. Burridge, Biol. Res.35, 239 (2002).

Sorted by citations

Items 501–600 of 1,034

The effect of substrate microtopography on focal adhesion maturation and actin organization via the RhoA/ROCK pathway
Seo, C. H., Furukawa, K. S., Montagne, K., Jeong, H., & Ushida, T. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.08.077 · Cited by 195
Perturbation of single hematopoietic stem cell fates in artificial niches
Lütolf, M. P., Doyonnas, R., Havenstrite, K. L., Koleckar, K., & Blau, H. M. (2008) · Integrative Biology · doi:10.1039/b815718a · Cited by 196
ECM stiffness primes the TGFβ pathway to promote chondrocyte differentiation
Allen, J. L., Cooke, M. E., & Alliston, T. N. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e12-03-0172 · Cited by 203
Cell cycle progression in confining microenvironments is regulated by a growth-responsive TRPV4-PI3K/Akt-p27 Kip1 signaling axis
Nam, S., Gupta, V. K., Lee, H.-P., Lee, J. Y., Wisdom, K. M., Varma, S., Flaum, E., Davis, C., West, R. B., & Chaudhuri, O. (2019) · Science Advances · doi:10.1126/sciadv.aaw6171 · Cited by 187
Murine and human pluripotent stem cell-derived cardiac bodies form contractile myocardial tissue in vitro
Kensah, G., Roa Lara, A., Dahlmann, J., Zweigerdt, R., Schwanke, K., Hegermann, J., Skvorc, D., Gawol, A., Azizian, A., Wagner, S., et al. (2013) · European Heart Journal · doi:10.1093/eurheartj/ehs349 · Cited by 193
Reversing bone loss by directing mesenchymal stem cells to bone
Yao, W., Guan, M., Jia, J., Dai, W., Lay, Y.-A. E., Amugongo, S. K., Liu, R., Olivos, D. J., Saunders, M. R., Lam, K. S., et al. (2013) · Stem Cells · doi:10.1002/stem.1461 · Cited by 193
Elastic Fully Three‐dimensional Microstructure Scaffolds for Cell Force Measurements
Klein, F., Striebel, T., Fischer, J., Jiang, Z., Franz, C. M., von Freymann, G., Wegener, M., & Bastmeyer, M. (2010) · Advanced Materials · doi:10.1002/adma.200902515 · Cited by 193
Polysaccharide-based polyelectrolyte multilayers
Crouzier, T., Boudou, T., & Picart, C. (2010) · Current Opinion in Colloid & Interface Science · doi:10.1016/j.cocis.2010.05.007 · Cited by 193
Collagen hydrogels incorporated with surface-aminated mesoporous nanobioactive glass: Improvement of physicochemical stability and mechanical properties is effective for hard tissue engineering
El‐Fiqi, A., Lee, J. H., Lee, E., & Kim, H. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.07.036 · Cited by 192
Tailoring the morphology of emulsion-templated porous polymers
Carnachan, R. J., Bokhari, M., Przyborski, S. A., & Cameron, N. R. (2006) · Soft Matter · doi:10.1039/b603211g · Cited by 193
Complex mechanics of the heterogeneous extracellular matrix in cancer
Malandrino, A., Mak, M., Kamm, R. D., & Moeendarbary, E. (2018) · Extreme Mechanics Letters · doi:10.1016/j.eml.2018.02.003 · Cited by 192
Substrate Topography Induces a Crossover from 2D to 3D Behavior in Fibroblast Migration
Ghibaudo, M., Trichet, L., Digabel, J. L., Richert, A., Hersen, P., & Ladoux, B. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2009.04.024 · Cited by 192
Matrix stiffness reverses the effect of actomyosin tension on cell proliferation
Mih, J. D., Marinković, А., Liu, F., Sharif, A. S., & Tschumperlin, D. J. (2012) · Journal of Cell Science · doi:10.1242/jcs.108886 · Cited by 203
Structure–mechanical property correlations of hydrogel forming β-sheet peptides
Rodriguez, L. M. D. L., Hémar, Y., Cornish, J., & Brimble, M. A. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00941c · Cited by 195
Materials roles for promoting angiogenesis in tissue regeneration
Lee, J., Parthiban, S. P., Jin, G., Knowles, J. C., & Kim, H. (2020) · Progress in Materials Science · doi:10.1016/j.pmatsci.2020.100732 · Cited by 194
Lung Parenchymal Mechanics
Suki, B., Stamenović, D., & Hubmayr, R. D. (2011) · Comprehensive physiology · doi:10.1002/cphy.c100033 · Cited by 234
Traction microscopy to identify force modulation in subresolution adhesions
Han, S. J., Oak, Y., Groisman, A., & Danuser, G. M. (2015) · Nature Methods · doi:10.1038/nmeth.3430 · Cited by 192
Liquid Crystalline Materials for Biological Applications
Lowe, A. M., & Abbott, N. L. (2011) · Chemistry of Materials · doi:10.1021/cm202632m · Cited by 191
Progress in cell-based therapies for tendon repair
Gaspar, D., Spanoudes, K., Holladay, C. A., Pandit, A. S., & Zeugolis, D. I. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.11.023 · Cited by 191
Intelligent Microfluidics: The Convergence of Machine Learning and Microfluidics in Materials Science and Biomedicine
Galan, E. A., Zhao, H., Wang, X., Dai, Q., Huck, W. T. S., & Ma, S. (2020) · Matter · doi:10.1016/j.matt.2020.08.034 · Cited by 194
Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces
Jeon, H., Koo, S.-M., Reese, W. M., Loskill, P., Grigoropoulos, C. P., & Healy, K. E. (2015) · Nature Materials · doi:10.1038/nmat4342 · Cited by 190
Local Cortical Tension by Myosin II Guides 3D Endothelial Cell Branching
Fischer, R. S., Gardel, M. L., Ma, X., Adelstein, R. S., & Waterman, C. M. (2009) · Current Biology · doi:10.1016/j.cub.2008.12.045 · Cited by 190
Scaling of Traction Forces with the Size of Cohesive Cell Colonies
Mertz, A. F., Banerjee, S., Che, Y., German, G. K., Xu, Y., Hyland, C., Marchetti, M. C., Horsley, V., & Dufresne, E. R. (2012) · Physical Review Letters · doi:10.1103/physrevlett.108.198101 · Cited by 190
Wide-range stiffness gradient PVA/HA hydrogel to investigate stem cell differentiation behavior
Oh, S. H., An, D. B., Kim, T. H., & Lee, J. H. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.02.016 · Cited by 191
Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory
Nasrollahi, S., Walter, C., Loza, A. J., Schimizzi, G. V., Longmore, G. D., & Pathak, A. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.09.012 · Cited by 196
Engineering microfluidic concentration gradient generators for biological applications
Toh, A. G. G., Wang, Z. P., Yang, C., & Nguyen, N. (2013) · Microfluidics and Nanofluidics · doi:10.1007/s10404-013-1236-3 · Cited by 200
Multi-material bio-fabrication of hydrogel cantilevers and actuators with stereolithography
Chan, V., Jeong, J. H., Bajaj, P., Collens, M. B., Saif, M. T. A., Kong, H., & Bashir, R. (2011) · Lab on a Chip · doi:10.1039/c1lc20688e · Cited by 187
The inner workings of stress fibers − from contractile machinery to focal adhesions and back
Livne, A., & Geiger, B. (2016) · Journal of Cell Science · doi:10.1242/jcs.180927 · Cited by 188
Electrically conductive graphene/polyacrylamide hydrogels produced by mild chemical reduction for enhanced myoblast growth and differentiation
Jo, H., Sim, M., Kim, S., Yang, S., Yoo, Y., Park, J., Yoon, T. H., Kim, M., & Lee, J. Y. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.10.035 · Cited by 190
Hydrogels that listen to cells: a review of cell-responsive strategies in biomaterial design for tissue regeneration
Ooi, H. W., Hafeez, S., van Blitterswijk, C., Moroni, L., & Baker, M. B. (2017) · Materials Horizons · doi:10.1039/c7mh00373k · Cited by 192
Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers
Jiu, Y., Lehtimäki, J. I., Tojkander, S., Cheng, F., Jäälinoja, H. T., Liu, X., Varjosalo, M. T., Eriksson, J., & Lappalainen, P. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.05.008 · Cited by 193
Biophysical control of invasive tumor cell behavior by extracellular matrix microarchitecture
Carey, S. P., Kraning-Rush, C. M., Williams, R. M., & Reinhart‐King, C. A. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.02.029 · Cited by 188
Control of three-dimensional substrate stiffness to manipulate mesenchymal stem cell fate toward neuronal or glial lineages
Her, G. J., Wu, H., Chen, M., Chen, M., Chang, S.-C., & Wang, T. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2012.10.012 · Cited by 189
The emergence of ECM mechanics and cytoskeletal tension as important regulators of cell function
Peyton, S. R., Ghajar, C. M., Khatiwala, C. B., & Putnam, A. J. (2007) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-007-0004-y · Cited by 191
Recent advances in engineering topography mediated antibacterial surfaces
Hasan, J. S., & Chatterjee, K. (2015) · Nanoscale · doi:10.1039/c5nr04156b · Cited by 187
Fibroblast mechanics in 3D collagen matrices☆
Rhee, S., & Grinnell, F. (2007) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2007.08.006 · Cited by 191
How cells sense extracellular matrix stiffness: a material's perspective
Trappmann, B., & Chen, C. S. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.03.020 · Cited by 187
Laminin and biomimetic extracellular elasticity enhance functional differentiation in mammary epithelia
Alcaraz, J., Xu, R., Mori, H., Nelson, C. M., Mroue, R. M., Spencer, V. A., Brownfield, D., Radisky, D. C., Bustamante, C., & Bissell, M. J. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.206 · Cited by 192
Fine-Tuning the Degree of Stem Cell Polarization and Alignment on Ordered Arrays of High-Aspect-Ratio Nanopillars
Bucaro, M. A., Vasquez, Y., Hatton, B. D., & Aizenberg, J. (2012) · ACS Nano · doi:10.1021/nn301654e · Cited by 187
3D printing of nanocellulose hydrogel scaffolds with tunable mechanical strength towards wound healing application
Xu, C., Molino, B. Z., Wang, X., Cheng, F., Xu, W., Molino, P. J., Bacher, M., Su, D., Rosenau, T., Willför, S. M., et al. (2018) · Journal of Materials Chemistry B · doi:10.1039/c8tb01757c · Cited by 190
The effect of time-dependent deformation of viscoelastic hydrogels on myogenic induction and Rac1 activity in mesenchymal stem cells
Cameron, A. R., Frith, J. E., Gomez, G. A., Yap, A. S., & Cooper-White, J. J. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.023 · Cited by 187
Cardiovascular Protection by ApoE and ApoE-HDL Linked to Suppression of ECM Gene Expression and Arterial Stiffening
Kothapalli, D., Liu, S., Bae, Y., Monslow, J., Xu, T., Hawthorne, E. A., Byfield, F. J., Castagnino, P. A., Rao, S. J., Rader, D. J., et al. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.09.018 · Cited by 187
Self-citation
Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers
Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., et al. (2021) · Nature Materials · doi:10.1038/s41563-021-00919-2 · Cited by 194
Electrical Stimulation Using Conductive Polymer Polypyrrole Promotes Differentiation of Human Neural Stem Cells: A Biocompatible Platform for Translational Neural Tissue Engineering
Stewart, E. M., Kobayashi, N., Higgins, M. J., Quigley, A. F., Jamali, S. S., Moulton, S. E., Kapsa, R. M. I., Wallace, G. G., & Crook, J. M. (2014) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2014.0338 · Cited by 185
Cell reorientation under cyclic stretching
Livne, A., Bouchbinder, E., & Geiger, B. (2014) · Nature Communications · doi:10.1038/ncomms4938 · Cited by 187
Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration
Celikkin, N., Mastrogiacomo, S., Jaroszewicz, J., Walboomers, X. F., & Święszkowski, W. (2017) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36226 · Cited by 183
Matrix Elasticity, Cytoskeletal Tension, and TGF-β: The Insoluble and Soluble Meet
Wells, R. G., & Discher, D. E. (2008) · Science Signaling · doi:10.1126/stke.110pe13 · Cited by 183
Self-citation
High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology
Yang, L., Pijuan‐Galito, S., Rho, H. S., Vasilevich, A. S., Eren, A. D., Ge, L., Habibović, P., Alexander, M. R., de Boer, J., Carlier, A., et al. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00752 · Cited by 191
Advanced electrospun hydrogel fibers for wound healing
Li, Y., Wang, J., Wang, Y., & Cui, W. (2021) · Composites Part B Engineering · doi:10.1016/j.compositesb.2021.109101 · Cited by 184
Self-citation
Recent progress in gellan gum hydrogels provided by functionalization strategies
Bacelar, A. H., Silva‐Correia, J., Oliveira, J. M., & Reis, R. L. (2016) · Journal of Materials Chemistry B · doi:10.1039/c6tb01488g · Cited by 183
Hydrogel substrate stress-relaxation regulates the spreading and proliferation of mouse myoblasts
Bauer, A., Gu, L., Kwee, B. J., Li, A. W., Dellacherie, M. O., Celiz, A. D., & Mooney, D. J. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.08.041 · Cited by 187
A Review of Bioactive Glass/Natural Polymer Composites: State of the Art
Sergi, R., Bellucci, D., & Cannillo, V. (2020) · Materials · doi:10.3390/ma13235560 · Cited by 193
Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring CellsPreprint
Bachir, A. I., Horwitz, A. R., Nelson, W. J., & Bianchini, J. M. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023234 · Cited by 191
From Nanotechnology to Nanomedicine: Applications to Cancer Research
Seigneuric, R., Markey, L., Nuyten, D. S. A., Dubernet, C., Evelo, C. T., Finot, É., & Garrido, C. (2010) · Current Molecular Medicine · doi:10.2174/156652410792630634 · Cited by 191
Role of Mechanical Factors in Fate Decisions of Stem Cells
Li, D., Zhou, J., Chowdhury, F., Cheng, J., Wang, N., & Wang, F. (2011) · Regenerative Medicine · doi:10.2217/rme.11.2 · Cited by 183
Quantitative Evaluation of Mechanosensing of Cells on Dynamically Tunable Hydrogels
Yoshikawa, H., Rossetti, F. F., Kaufmann, S., Kaindl, T., Madsen, J., Engel, U., Lewis, A. L., Armes, S. P., & Tanaka, M. (2011) · Journal of the American Chemical Society · doi:10.1021/ja1060615 · Cited by 183
Inverse Opal Scaffolds and Their Biomedical Applications
Zhang, Y. S., Zhu, C., & Younan Xia, A. (2017) · Advanced Materials · doi:10.1002/adma.201701115 · Cited by 183
Lyophilized Silk Sponges: A Versatile Biomaterial Platform for Soft Tissue Engineering
Rnjak‐Kovacina, J., Wray, L. S., Burke, K. A., Torregrosa, T., Golinski, J., Huang, W., & Kaplan, D. L. (2015) · ACS Biomaterials Science & Engineering · doi:10.1021/ab500149p · Cited by 181
Particle tracking in living cells: a review of the mean square displacement method and beyond
Gal, N., Lechtman-Goldstein, D., & Weihs, D. (2013) · Rheologica Acta · doi:10.1007/s00397-013-0694-6 · Cited by 179
Enhancement of bio-stability and mechanical properties of hyaluronic acid hydrogels by tannic acid treatment
Lee, H.-Y., Hwang, C., Kim, H., & Jeong, S. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.01.056 · Cited by 179
A biomaterial with a channel-like pore architecture induces endochondral healing of bone defects
Petersen, A., Princ, A., Korus, G., Ellinghaus, A., Leemhuis, H., Herrera, A., Klaumünzer, A., Schreivogel, S., Woloszyk, A., Schmidt‐Bleek, K., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-06504-7 · Cited by 187
Advances in Engineering Human Tissue Models
Moysidou, C., Barberio, C., & Owens, R. M. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.620962 · Cited by 193
Mechanosensing and Mechanoregulation of Endothelial Cell Functions
Fang, Y. H., Wu, D., & Birukov, K. G. (2019) · Comprehensive physiology · doi:10.1002/cphy.c180020 · Cited by 222
Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus
Wang, H., Haeger, S. M., Kloxin, A. M., Leinwand, L. A., & Anseth, K. S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0039969 · Cited by 182
Actin cytoskeleton in myofibroblast differentiation: Ultrastructure defining form and driving function
Sandbo, N. K., & Dulin, N. O. (2011) · Translational research · doi:10.1016/j.trsl.2011.05.004 · Cited by 185
Trabecular meshwork stiffness in glaucoma
Wang, K., Read, A. T., Sulchek, T., & Ethier, C. R. (2016) · Experimental Eye Research · doi:10.1016/j.exer.2016.07.011 · Cited by 180
Current tissue engineering and novel therapeutic approaches to axonal regeneration following spinal cord injury using polymer scaffolds
Madigan, N. N., McMahon, S. S., O’Brien, T., Yaszemski, M. J., & Windebank, A. J. (2009) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2009.08.015 · Cited by 182
Tuning the Elastic Modulus of Hydrated Collagen Fibrils
Grant, C. A., Brockwell, D. J., Radford, S. E., & Thomson, N. H. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2009.09.010 · Cited by 179
Tenogenic Induction of Human MSCs by Anisotropically Aligned Collagen Biotextiles
Younesi, M., Islam, A., Kishore, V., Anderson, J. M., & Akkuş, O. (2014) · Advanced Functional Materials · doi:10.1002/adfm.201400828 · Cited by 179
TGF-β1-SOX9 axis-inducible COL10A1 promotes invasion and metastasis in gastric cancer via epithelial-to-mesenchymal transition
Li, T., Huang, H., Shi, G., Zhao, L., Li, T., Zhang, Z., Liu, R., Hu, Y., Liu, H., Yu, J., et al. (2018) · Cell Death and Disease · doi:10.1038/s41419-018-0877-2 · Cited by 180
Hyaluronic Acid: Incorporating the Bio into the Material
Wolf, K. J., & Kumar, S. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b01268 · Cited by 178
Fmoc-diphenylalanine hydrogels: understanding the variability in reported mechanical properties
Raeburn, J., Pont, G., Chen, L., Cesbron, Y., Lévy, R., & Adams, D. J. (2011) · Soft Matter · doi:10.1039/c1sm06929b · Cited by 177
Elastic clues in cancer detection
Suresh, S. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.397 · Cited by 175
Osteoinductive hydroxyapatite-coated titanium implants
Ripamonti, U., Roden, L. C., & Renton, L. F. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.01.050 · Cited by 177
Evaluating Drug Efficacy and Toxicology in Three Dimensions: Using Synthetic Extracellular Matrices in Drug Discovery
Prestwich, G. D. (2007) · Accounts of Chemical Research · doi:10.1021/ar7000827 · Cited by 176
Tissue engineering with gellan gum
Stevens, L. R., Gilmore, K. J., Wallace, G. G., & Panhuis, M. I. H. (2016) · Biomaterials Science · doi:10.1039/c6bm00322b · Cited by 177
Mechanical tension contributes to clustering of neurotransmitter vesicles at presynaptic terminals
Siechen, S., Yang, S., Chiba, A., & Saif, M. T. A. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0901867106 · Cited by 176
Self-Healing, Self-Assembled β-Sheet Peptide–Poly(γ-glutamic acid) Hybrid Hydrogels
Clarke, D. E., Pashuck, E. T., Bertazzo, S., Weaver, J. V. M., & Stevens, M. M. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.7b00528 · Cited by 176
Understanding the extracellular forces that determine cell fate and maintenance
Kumar, A., Placone, J. K., & Engler, A. J. (2017) · Development · doi:10.1242/dev.158469 · Cited by 175
Traumatic Brain Injury and the Neuronal Microenvironment: A Potential Role for Neuropathological Mechanotransduction
Hemphill, M. A., Dauth, S., Yu, C. J., Dabiri, B. E., & Parker, K. K. (2015) · Neuron · doi:10.1016/j.neuron.2015.02.041 · Cited by 180
Comprehensive proteomic characterization of stem cell-derived extracellular matrices
Ragelle, H., Naba, A., Larson, B. L., Zhou, F., Miralem, P., Whittaker, C. A., Rosario, A. M. D., Langer, R., Hynes, R. O., & Anderson, D. G. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.03.008 · Cited by 176
Ultra-durable cell-free bioactive hydrogel with fast shape memory and on-demand drug release for cartilage regeneration
Yang, Y., Zhao, X., Wang, S., Zhang, Y., Yang, A., Cheng, Y., & Chen, X. (2023) · Nature Communications · doi:10.1038/s41467-023-43334-8 · Cited by 182
Aging and ocular tissue stiffness in glaucoma
Liu, B., McNally, S., Kilpatrick, J. I., Jarvis, S., & O'Brien, C. J. (2017) · Survey of Ophthalmology · doi:10.1016/j.survophthal.2017.06.007 · Cited by 175
Designer biomaterials for mechanobiology
Li, L., Eyckmans, J., & Chen, C. S. (2017) · Nature Materials · doi:10.1038/nmat5049 · Cited by 183
Ultrastretchable Kirigami Bioprobes
Morikawa, Y., Yamagiwa, S., Sawahata, H., Numano, R., Koida, K., Ishida, M., & Kawano, T. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201701100 · Cited by 175
Biomechanical Forces Shape the Tumor Microenvironment
Shieh, A. C. (2011) · Annals of Biomedical Engineering · doi:10.1007/s10439-011-0252-2 · Cited by 176
Front–Rear Polarization by Mechanical Cues: From Single Cells to Tissues
Ladoux, B., Mège, R., & Trepat, X. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.02.002 · Cited by 182
Bone Marrow Mesenchymal Stem Cells in a Three-Dimensional Gelatin Sponge Scaffold Attenuate Inflammation, Promote Angiogenesis, and Reduce Cavity Formation in Experimental Spinal Cord Injury
Zeng, X., Zeng, Y., Ma, Y., Lu, L., Du, B.-L., Zhang, W., Li, Y., & Chan, W. Y. (2011) · Cell Transplantation · doi:10.3727/096368911x566181 · Cited by 172
Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer
Zhu, B., Luo, S., Zhao, H., Lin, H., Sekine, J., Nakao, A., Chen, C., Yamashita, Y., & Yu, H. (2014) · Nature Communications · doi:10.1038/ncomms5523 · Cited by 177
Rapid Prototyping of Arrayed Microfluidic Systems in Polystyrene for Cell-Based Assays
Young, E. W. K., Berthier, E., Guckenberger, D. J., Sackmann, E. K., Lamers, C., Meyvantsson, I., Huttenlocher, A., & Beebe, D. J. (2011) · Analytical Chemistry · doi:10.1021/ac102897h · Cited by 177
Bioprinting of a Cell-Laden Conductive Hydrogel Composite
Spencer, A. R., Sani, E. S., Soucy, J. R., Corbet, C. C., Primbetova, A., Koppes, R. A., & Annabi, N. (2019) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b07353 · Cited by 172
Regulation of Mitochondrial Structure and Dynamics by the Cytoskeleton and Mechanical Factors
Bartolák‐Suki, E., Imsirovic, J., Nishibori, Y., Krishnan, R., & Suki, B. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18081812 · Cited by 176
Engineering 3D Hydrogels for Personalized In Vitro Human Tissue Models
Liaw, C., Ji, S., & Güvendiren, M. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701165 · Cited by 171
Mechanical Mapping of Spinal Cord Growth and Repair in Living Zebrafish Larvae by Brillouin Imaging
Schlüßler, R., Möllmert, S., Abuhattum, S., Cojoc, G., Müller, P. F., Kim, K., Möckel, C., Zimmermann, C., Czarske, J. W., & Guck, J. R. (2018) · Biophysical Journal · doi:10.1016/j.bpj.2018.07.027 · Cited by 171
Viscoelasticity in natural tissues and engineered scaffolds for tissue reconstruction
Huang, D., Huang, Y. X., Xiao, Y., Yang, X., Lin, H., Feng, G., Zhu, X., & Zhang, X. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.08.013 · Cited by 177
Designing Hydrogels for 3D Cell Culture Using Dynamic Covalent Crosslinking
Rizwan, M., Baker, A. E. G., & Shoichet, M. S. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202100234 · Cited by 177
A Balance of Substrate Mechanics and Matrix Chemistry Regulates Endothelial Cell Network Assembly
Califano, J. P., & Reinhart‐King, C. A. (2008) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-008-0022-x · Cited by 173
Control of bacterial biofilm growth on surfaces by nanostructural mechanics and geometry
Epstein, A. K., Hochbaum, A. I., Kim, P., & Aizenberg, J. (2011) · Nanotechnology · doi:10.1088/0957-4484/22/49/494007 · Cited by 169
Quantitative structural mechanobiology of platelet-driven blood clot contraction
Kim, O. V., Игоревич, Л. Р., Alber, M., & Weisel, J. W. (2017) · Nature Communications · doi:10.1038/s41467-017-00885-x · Cited by 169
Synthesis and evaluation of alginate, gelatin, and hyaluronic acid hybrid hydrogels for tissue engineering applications
Serafin, A., Culebras, M., & Collins, M. N. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.123438 · Cited by 169

Authors: Discher, D. E., Janmey, P., & Wang, Y.-L.
Affiliations: University of Massachusetts Chan Medical School (US)
Corresponding author: Discher, Dennis E.
ORCID: Discher, Dennis E.
Janmey, Paul
Wang, Yu-li
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2005-11-18
Volume: 310
Issue: 5751
Pages: 1139-1143
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1116995
OpenAlex: W2054635074
Cited by: 6,382
References: 82
Citations by year: 2026: 287 · 2025: 269 · 2024: 239 · 2023: 237 · 2022: 318 · 2021: 269 · 2020: 369 · 2019: 389 · 2018: 399 · 2017: 432 · 2016: 379 · 2015: 392 · 2014: 407 · 2013: 369 · 2012: 380
FWCI: 9.07
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Keywords: substrate stiffness, mechanotransduction, cell-matrix adhesion, cell-substrate adhesion, soft agar, cancer cell detection, fibroblasts, muscle cells, neurons, actomyosin cytoskeleton, adhesion complexes, cell contractility, tissue stiffness, cell differentiation, tissue regeneration
MeSH terms: Biomechanical Phenomena, Cell Adhesion, Cell Communication, Cell Physiological Phenomena, Cytoskeleton, Elasticity, Humans, Muscle Contraction, Organogenesis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Tissue Cells Feel and Respond to the Stiffness of Their Substrate

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks