Cited by 6,382Cellular Mechanics and InteractionsForce Microscopy Techniques and ApplicationsHydrogels: synthesis, properties, applications

The DOI matches its Crossref record; 82 of the 82 references it cites were found in a registry or an index.

Tissue Cells Feel and Respond to the Stiffness of Their Substrate

Discher, Dennis E., Janmey, Paul, Wang, Yu-li·2005·Science

DOI 10.1126/science.1116995

Open this paper in CoChat — free account

Abstract

Normal tissue cells are generally not viable when suspended in a fluid and are therefore said to be anchorage dependent. Such cells must adhere to a solid, but a solid can be as rigid as glass or softer than a baby's skin. The behavior of some cells on soft materials is characteristic of important phenotypes; for example, cell growth on soft agar gels is used to identify cancer cells. However, an understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels (or to other cells) with which elasticity can be tuned to approximate that of tissues. Key roles in molecular pathways are played by adhesion complexes and the actinmyosin cytoskeleton, whose contractile forces are transmitted through transcellular structures. The feedback of local matrix stiffness on cell state likely has important implications for development, differentiation, disease, and regeneration.

Integrity signals

Registry

Crossref · 10.1126/science.1116995

Cites

82 of 82 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

New Perspectives in Cell Adhesion: RGD and Integrins
Ruoslahti, E. I., & Pierschbacher, M. D. (1987) · Science · doi:10.1126/science.2821619 · Cited by 4,875
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Self-citation
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,082
Self-citation
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Exploring and Engineering the Cell Surface Interface
Stevens, M. M., & George, J. H. (2005) · Science · doi:10.1126/science.1106587 · Cited by 2,607
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Self-citation
Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
Balaban, N. Q., Schwarz, U. S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., Mahalu, D., Safran, S. A., Bershadsky, A. D., Addadi, L., et al. (2001) · Nature Cell Biology · doi:10.1038/35074532 · Cited by 2,137
Cells lying on a bed of microneedles: An approach to isolate mechanical force
Tan, J. L., Tien, J., Pirone, D. M., Gray, D. S., Bhadriraju, K., & Chen, C. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0235407100 · Cited by 2,002
Myotubes differentiate optimally on substrates with tissue-like stiffness
Engler, A. J., Griffin, M. A., Sen, S., Bönnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200405004 · Cited by 1,743
Self-citation
Nonlinear elasticity in biological gels
Storm, C., Pastore, J. J., MacKintosh, F. C., Lubensky, T. C., & Janmey, P. A. (2005) · Nature · doi:10.1038/nature03521 · Cited by 1,707
Self-citation

Show all 82 references

Research works printed but not linked: 5

D. R. Welch et al., Cancer Res.60, 1552 (2000).
F. J. Alenghat, D. E. Ingber, Sci. STKE119, pe6 (2002).
A. Saez A. Buguin P. Silberzan B. Ladoux Biophys J. in press.
J. P. Trinkaus, J. P. Lentz, Dev. Biol.89, 115 (1964).
W. T. Arthur, N. K. Noren, K. Burridge, Biol. Res.35, 239 (2002).

Sorted by citations

Items 701–800 of 1,034

The influence of scaffold elasticity on germ layer specification of human embryonic stem cells
Zoldan, J., Karagiannis, E. D., Lee, C. Y., Anderson, D. G., Langer, R., & Levenberg, S. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.09.012 · Cited by 144
Substrate rigidity regulates Ca2+ oscillation via RhoA pathway in stem cells
Kim, T., Seong, J., Ouyang, M., Sun, J., Lu, S., Hong, J. P., Wang, N., & Wang, Y. (2008) · Journal of Cellular Physiology · doi:10.1002/jcp.21598 · Cited by 146
Self-citation
Mechanical Regulation of Nuclear Structure and Function
Martins, R. P., Finan, J. D., Guilak, F., & Lee, D. A. (2012) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124638 · Cited by 151
Three-dimensional cancer models mimic cell-matrix interactions in the tumour microenvironment
Herrmann, D., Conway, J. R. W., Vennin, C., Magenau, A., Hughes, W. E., Morton, J. P., & Timpson, P. (2014) · Carcinogenesis · doi:10.1093/carcin/bgu108 · Cited by 144
Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior
Zeiger, A. S., Loe, F. C., Li, R., Raghunath, M., & Vliet, K. J. V. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037904 · Cited by 145
The use of type 1 collagen scaffold containing stromal cell-derived factor-1 to create a matrix environment conducive to partial-thickness cartilage defects repair
Zhang, W., Chen, J., Tao, J., Jiang, Y., Hu, C., Huang, L., Ji, J., & Ouyang, H. W. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.10.027 · Cited by 143
Highly elastic and suturable electrospun poly(glycerol sebacate) fibrous scaffolds
Jeffries, E. M., Allen, R., Gao, J., Pesce, M., & Wang, Y. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.02.005 · Cited by 143
Self-citation
Screening of rat mesenchymal stem cell behaviour on polydimethylsiloxane stiffness gradients
Wang, P., Tsai, W., & Voelcker, N. H. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.09.030 · Cited by 145
Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractility
Martinez-Rico, C., Pincet, F., Thiery, J. P., & Dufour, S. (2010) · Journal of Cell Science · doi:10.1242/jcs.047878 · Cited by 143
A toolbox to explore the mechanics of living embryonic tissues
Campàs, O. (2016) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2016.03.011 · Cited by 151
Silk fibroin/collagen protein hybrid cell-encapsulating hydrogels with tunable gelation and improved physical and biological properties
Buitrago, J. O., Patel, K. D., El‐Fiqi, A., Lee, J., Kundu, B., Lee, H., & Kim, H. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.12.026 · Cited by 147
Vascular endothelial growth factor and substrate mechanics regulate in vitro tubulogenesis of endothelial progenitor cells
Hanjaya‐Putra, D., Yee, J., Ceci, D., Truitt, R., Yee, D., & Gerecht, S. (2009) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2009.00981.x · Cited by 145
Biocompatible, degradable thermoplastic polyurethane based on polycaprolactone-block-polytetrahydrofuran-block-polycaprolactone copolymers for soft tissue engineering
Mi, H., Jing, X., Napiwocki, B. N., Hagerty, B. S., Chen, G., & Turng, L. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb00419b · Cited by 142
Microfluidic Skin‐on‐a‐Chip Models: Toward Biomimetic Artificial Skin
Sutterby, E., Thurgood, P., Baratchi, S., Khoshmanesh, K., & Pirogova, E. (2020) · Small · doi:10.1002/smll.202002515 · Cited by 142
Sensitivity of alveolar macrophages to substrate mechanical and adhesive properties
Féréol, S., Fodil, R., Labat, B., Galiacy, S., Laurent, V. M., Louis, B., Isabey, D., & Planus, E. (2006) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20130 · Cited by 139
Fabrication and optimization of alginate hydrogel constructs for use in 3D neural cell culture
Frampton, J. P., Hynd, M. R., Shuler, M. L., & Shain, W. G. (2011) · Biomedical Materials · doi:10.1088/1748-6041/6/1/015002 · Cited by 141
Modified gellan gum hydrogels for tissue engineering applications
Ferris, C. J., Gilmore, K. J., Wallace, G. G., & Panhuis, M. I. H. (2013) · Soft Matter · doi:10.1039/c3sm27389j · Cited by 141
Living Materials Herald a New Era in Soft Robotics
Appiah, C., Arndt, C., Siemsen, K., Heitmann, A., Staubitz, A., & Selhuber‐Unkel, C. (2019) · Advanced Materials · doi:10.1002/adma.201807747 · Cited by 144
Cortical cell stiffness is independent of substrate mechanics
Rheinlaender, J., Dimitracopoulos, A., Wallmeyer, B., Kronenberg, N. M., Chalut, K. J., Gather, M. C., Betz, T., Charras, G., & Franze, K. (2020) · Nature Materials · doi:10.1038/s41563-020-0684-x · Cited by 141
Physico-mechanical aspects of extracellular matrix influences on tumorigenic behaviors
Cukierman, E., & Bassi, D. (2010) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2010.04.004 · Cited by 144
Electrochemically Controlled Swelling and Mechanical Properties of a Polymer Nanocomposite
Schmidt, D. J., Cebeci, F. Ç., Kalcioglu, Z. I., Wyman, S. G., Ortiz, C., Vliet, K. J. V., & Hammond, P. T. (2009) · ACS Nano · doi:10.1021/nn900526c · Cited by 140
In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry
Nichols, J. E., Cortiella, J., Lee, J., Niles, J. A., Cuddihy, M. J., Wang, S., Bielitzki, J. T., Cantu, A., Mlcak, R. P., Valdivia, E., et al. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.10.041 · Cited by 140
On the biomechanical function of scaffolds for engineering load-bearing soft tissues
Stella, J. A., D’Amore, A., Wagner, W. R., & Sacks, M. S. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.01.001 · Cited by 141
Regulation of wound healing and organ fibrosis by toll-like receptors
Huebener, P., & Schwabe, R. F. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2012.11.017 · Cited by 140
Bending forces plastically deform growing bacterial cell walls
Amir, A., Babaeipour, F., McIntosh, D. B., Nelson, D. R., & Jun, S. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1317497111 · Cited by 140
Hydrophilic elastomeric biomaterials based on resilin-like polypeptides
Charati, M. B., Ifkovits, J. L., Burdick, J. A., Linhardt, J. G., & Kiick, K. L. (2009) · Soft Matter · doi:10.1039/b910980c · Cited by 140
Cell softness regulates tumorigenicity and stemness of cancer cells
Lv, J., Liu, Y., Cheng, F., Li, J., Zhou, Y., Zhang, T., Zhou, N., Li, C., Wang, Z., Ma, L., et al. (2020) · The EMBO Journal · doi:10.15252/embj.2020106123 · Cited by 142
Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
Hiratsuka, K., Miyoshi, T., Kroll, K. T., Gupta, N. R., Valerius, M. T., Ferrante, T., Yamashita, M., Lewis, J. A., & Morizane, R. (2022) · Science Advances · doi:10.1126/sciadv.abq0866 · Cited by 140
Biocompatibility of amphiphilic diblock copolypeptide hydrogels in the central nervous system
Yang, C.-Y., Song, B., Ao, Y., Nowak, A. P., Abelowitz, R. B., Korsak, R. A., Havton, L. A., Deming, T. J., & Sofroniew, M. V. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.01.056 · Cited by 139
Dynamic force-induced direct dissociation of protein complexes in a nuclear body in living cells
Poh, Y., Shevtsov, S. P., Chowdhury, F., Wu, D. C., Na, S., Dundr, M., & Wang, N. (2012) · Nature Communications · doi:10.1038/ncomms1873 · Cited by 147
Fabrication of 2D protein microstructures and 3D polymer–protein hybrid microstructures by two-photon polymerization
Engelhardt, S., Hoch, E., Borchers, K., Meyer, W., Krüger, H., Tovar, G. E. M., & Gillner, A. (2011) · Biofabrication · doi:10.1088/1758-5082/3/2/025003 · Cited by 141
Substrate stiffness regulates the proliferation, migration, and differentiation of epidermal cells
Wang, Y., Wang, G., Luo, X., Qiu, J., & Tang, C. (2011) · Burns · doi:10.1016/j.burns.2011.09.002 · Cited by 140
Self-citation
Vertically Aligned Carbon Nanofiber Architecture as a Multifunctional 3-D Neural Electrical Interface
Nguyen-Vu, T. B., Chen, H., Cassell, A. M., Andrews, R. J., Meyyappan, M., & Li, J. (2007) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2007.891169 · Cited by 140
Mesenchymal stem cell mechanobiology and emerging experimental platforms
MacQueen, L. A., Sun, Y., & Simmons, C. A. (2013) · Journal of The Royal Society Interface · doi:10.1098/rsif.2013.0179 · Cited by 142
The Regulation of Cellular Responses to Mechanical Cues by Rho GTPases
Hoon, J.-L., Tan, M., & Koh, C.-G. (2016) · Cells · doi:10.3390/cells5020017 · Cited by 151
Designing stem cell niches for differentiation and self-renewal
Donnelly, H., Salmerón‐Sánchez, M., & Dalby, M. J. (2018) · Journal of The Royal Society Interface · doi:10.1098/rsif.2018.0388 · Cited by 142
TRPV4—A Missing Link Between Mechanosensation and Immunity
Michalick, L., & Kuebler, W. M. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.00413 · Cited by 144
Universality in cell mechanics
Trepat, X., Lenormand, G., & Fredberg, J. J. (2008) · Soft Matter · doi:10.1039/b804866e · Cited by 143
Effects of surface molecular chirality on adhesion and differentiation of stem cells
Yao, X., Hu, Y., Cao, B., Peng, R., & Ding, J. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.08.013 · Cited by 138
Real-time single-cell response to stiffness
Mitrossilis, D., Fouchard, J., Pereira, D., Postic, F., Richert, A., Jean, M. S., & Asnacios, A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1007940107 · Cited by 138
Rapid disorganization of mechanically interacting systems of mammary acini
Shi, Q., Ghosh, R. P., Engelke, H., Rycroft, C. H., Cassereau, L., Sethian, J. A., Weaver, V. M., & Liphardt, J. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1311312110 · Cited by 139
Tuning the Poisson's Ratio of Biomaterials for Investigating Cellular Response
Zhang, W., Soman, P., Meggs, K., Qu, X., & Chen, S. (2013) · Advanced Functional Materials · doi:10.1002/adfm.201202666 · Cited by 145
Interactions at scaffold interfaces: Effect of surface chemistry, structural attributes and bioaffinity
Dave, K., & Gomes, V. G. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.110078 · Cited by 142
Meta-biomaterials
ZADPOOR, A. A. (2019) · Biomaterials Science · doi:10.1039/c9bm01247h · Cited by 144
Algal Polysaccharides-Based Hydrogels: Extraction, Synthesis, Characterization, and Applications
Lin, J., Jiao, G., & Kermanshahi‐pour, A. (2022) · Marine Drugs · doi:10.3390/md20050306 · Cited by 146
Encapsulation of fibroblasts causes accelerated alginate hydrogel degradation
Hunt, N. C., Smith, A. M., Gbureck, U., Shelton, R. M., & Grover, L. M. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.03.026 · Cited by 137
Dynamics and regulation of contractile actin–myosin networks in morphogenesis
Kasza, K. E., & Zallen, J. A. (2010) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2010.10.014 · Cited by 148
Biphasic response of cell invasion to matrix stiffness in three-dimensional biopolymer networks
Lang, N. R., Skodzek, K., Hurst, S., Mainka, A., Steinwachs, J., Schneider, J. R., Aifantis, K. E., & Fabry, B. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.11.003 · Cited by 137
Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
Abagnale, G., Sechi, A. S., Steger, M., Zhou, Q., Kuo, C., Aydın, G., Schalla, C., Müller‐Newen, G., Zenke, M., Costa, I. G., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.06.016 · Cited by 137
Nanoparticle–Cell Interaction: A Cell Mechanics Perspective
Septiadi, D., Crippa, F., Moore, T. L., Rothen‐Rutishauser, B., & Petri‐Fink, A. (2018) · Advanced Materials · doi:10.1002/adma.201704463 · Cited by 142
Dispersible hydrogel force sensors reveal patterns of solid mechanical stress in multicellular spheroid cultures
Lee, W., Kalashnikov, N., Mok, S., Halaoui, R., Kuzmin, E., Putnam, A. J., Takayama, S., Park, M., McCaffrey, L. M., Zhao, R., et al. (2019) · Nature Communications · doi:10.1038/s41467-018-07967-4 · Cited by 135
Mechanotransduction: from the cell surface to the nucleus via RhoA
Burridge, K., Monaghan-Benson, E., & Graham, D. M. (2019) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2018.0229 · Cited by 146
Mineralized collagen plywood contributes to bone autograft performance
Robin, M., Mouloungui, E., Castillo-Dalí, G., Wang, Y., Jl, S., Pavon‐Djavid, G., Divoux, T., Manneville, S., Behr, L., Cardi, D., et al. (2024) · Nature · doi:10.1038/s41586-024-08208-z · Cited by 137
Self-citation
Engineering strategies to mimic the glioblastoma microenvironment
Rape, A. D., Ananthanarayanan, B., & Kumar, S. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.08.012 · Cited by 138
Paper-based cell culture platform and its emerging biomedical applications
Ng, K., Gao, B., Yong, K. W., Li, Y., Shi, M., Zhao, X., Li, Z., Zhang, X. H., Pingguan‐Murphy, B., Yang, H., et al. (2016) · Materials Today · doi:10.1016/j.mattod.2016.07.001 · Cited by 138
Live-cell super-resolved PAINT imaging of piconewton cellular traction forces
Brockman, J. M., Su, H., Blanchard, A. T., Duan, Y., Meyer, T. A., Quach, M. E., Glazier, R., Bazrafshan, A. S., Bender, R. L., Kellner, A. V., et al. (2020) · Nature Methods · doi:10.1038/s41592-020-0929-2 · Cited by 136
B Cell Activation Is Regulated by the Stiffness Properties of the Substrate Presenting the Antigens
Wan, Z., Zhang, S., Fan, Y., Liu, K., Du, F., Davey, A. M., Zhang, H., Han, W., Xiong, C., & Liu, W. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1202976 · Cited by 137
Study of cellular behaviors on concave and convex microstructures fabricated from elastic PDMS membranes
Park, J. Y., Lee, D. H., Lee, E. J., & Lee, S. (2009) · Lab on a Chip · doi:10.1039/b820955c · Cited by 135
Probing the compressibility of tumor cell nuclei by combined atomic force–confocal microscopy
Krause, M., Riet, J. T., & Wolf, K. (2013) · Physical Biology · doi:10.1088/1478-3975/10/6/065002 · Cited by 142
A Writable Polypeptide–DNA Hydrogel with Rationally Designed Multi‐modification Sites
Li, C., Chen, P., Shao, Y., Zhou, X., Wu, Y., Yang, Z., Li, Z., Weil, T., & Liu, D. (2014) · Small · doi:10.1002/smll.201401906 · Cited by 135
The Yin-Yang of Rigidity Sensing: How Forces and Mechanical Properties Regulate the Cellular Response to Materials
Schoen, I., Pruitt, B. L., & Viola Vogel, B. (2013) · Annual Review of Materials Research · doi:10.1146/annurev-matsci-062910-100407 · Cited by 134
Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix
Mekhdjian, A. H., Kai, F., Rubashkin, M. G., Prahl, L. S., Przybyla, L., McGregor, A., Bell, E. S., Barnes, J. M., DuFort, C. C., Ou, G., et al. (2017) · Molecular Biology of the Cell · doi:10.1091/mbc.e16-09-0654 · Cited by 140
Hierarchical Nanowire Arrays as Three-Dimensional Fractal Nanobiointerfaces for High Efficient Capture of Cancer Cells
Zhang, F., Jiang, Y. L., Liu, X., Meng, J., Zhang, P., Liu, H., Gao, Y., Li, G., Jiang, L., Wan, L., et al. (2015) · Nano Letters · doi:10.1021/acs.nanolett.5b04731 · Cited by 134
Mechanical properties and cellular response of novel electrospun nanofibers for ligament tissue engineering: Effects of orientation and geometry
Pauly, H. M., Kelly, D. J., Popat, K. C., Trujillo, N. A., Dunne, N., McCarthy, H. O., & Donahue, T. L. H. (2016) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2016.03.022 · Cited by 134
Biophysical regulation of macrophages in health and disease
Meli, V. S., Veerasubramanian, P. K., Atcha, H., Reitz, Z., Downing, T. L., & Liu, W. F. (2019) · Journal of Leukocyte Biology · doi:10.1002/jlb.mr0318-126r · Cited by 133
Network connectivity, mechanical properties and cell adhesion for hyaluronic acid/PEG hydrogels
Ouasti, S., Donno, R., Cellesi, F., Sherratt, M. J., Terenghi, G., & Tirelli, N. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.05.044 · Cited by 133
A review of the effects of the cell environment physicochemical nanoarchitecture on stem cell commitment
Das, R. K., & Zouani, O. F. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.03.044 · Cited by 133
Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients
Shamloo, A., & Heilshorn, S. C. (2010) · Lab on a Chip · doi:10.1039/c005069e · Cited by 134
Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
Liu, F., & Tschumperlin, D. J. (2011) · Journal of Visualized Experiments · doi:10.3791/2911 · Cited by 133
The activation of IgM- or isotype-switched IgG- and IgE-BCR exhibits distinct mechanical force sensitivity and threshold
Wan, Z., Chen, X., Chen, H., Ji, Q., Chen, Y., Wang, J., Cao, Y., Wang, F., Lou, J., Tang, Z., et al. (2015) · eLife · doi:10.7554/elife.06925 · Cited by 141
Insight on stem cell preconditioning and instructive biomaterials to enhance cell adhesion, retention, and engraftment for tissue repair
Shafiq, M., Jung, Y., & Kim, S. H. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.03.020 · Cited by 136
Optimizing Blended Collagen-Fibrin Hydrogels for Cardiac Tissue Engineering with Human iPSC-derived Cardiomyocytes
Kaiser, N. J., Kant, R. J., Minor, A. J., & Coulombe, K. L. K. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b01112 · Cited by 132
T-cell activation is modulated by the 3D mechanical microenvironment
Majedi, F. S., Hasani‐Sadrabadi, M. M., Thauland, T. J., Li, S., Bouchard, L., & Butte, M. J. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120058 · Cited by 132
3D anisotropic photocatalytic architectures as bioactive nerve guidance conduits for peripheral neural regeneration
Zhang, Z., Jørgensen, M. L., Wang, Z., Amagat, J., Wang, Y., Li, Q., Dong, M., & Chen, M. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120108 · Cited by 133
Self-citation
Natural Biomaterials for Cardiac Tissue Engineering: A Highly Biocompatible Solution
Majid, Q. A., Fricker, A. T. R., Gregory, D. A., Davidenko, N., Hernández‐Cruz, O., Jabbour, R. J., Owen, T. J., Basnett, P., Lukasiewicz, B., Stevens, M. M., et al. (2020) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2020.554597 · Cited by 138
Engineering the Follicle Microenvironment
West, E. R., Shea, L. D., & Woodruff, T. K. (2007) · Seminars in Reproductive Medicine · doi:10.1055/s-2007-980222 · Cited by 133
Modeling cell rheology with the Subcellular Element Model
Sandersius, S. A., & Newman, T. J. (2008) · Physical Biology · doi:10.1088/1478-3975/5/1/015002 · Cited by 133
The effect of culture conditions on the adipogenic and osteogenic inductions of mesenchymal stem cells on micropatterned surfaces
Peng, R., Yao, X., Cao, B., Tang, J., & Ding, J. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.05.010 · Cited by 132
A new lock-step mechanism of matrix remodelling based on subcellular contractile events
Castella, L. F., Buscemi, L., Godbout, C., Meister, J.-J., & Hinz, B. (2010) · Journal of Cell Science · doi:10.1242/jcs.066795 · Cited by 132
Controlled two-photon photodegradation of PEG hydrogels to study and manipulate subcellular interactions on soft materials
Tibbitt, M., Kloxin, A. M., Dyamenahalli, K., & Anseth, K. (2010) · Soft Matter · doi:10.1039/c0sm00174k · Cited by 123
Mimicking Cellular Environments by Nanostructured Soft Interfaces
Gräter, S., Huang, J., Perschmann, N., López‐García, M., Kessler, H., Ding, J., & Spatz, J. P. (2007) · Nano Letters · doi:10.1021/nl070098g · Cited by 132
Normal and Fibrotic Rat Livers Demonstrate Shear Strain Softening and Compression Stiffening: A Model for Soft Tissue Mechanics
Perepelyuk, M., Chin, L., Cao, X., van Oosten, A., Shenoy, V. B., Janmey, P. A., & Wells, R. G. (2016) · PLoS ONE · doi:10.1371/journal.pone.0146588 · Cited by 129
Self-citation
Polysaccharides as Cell Carriers for Tissue Engineering: the Use of Cellulose in Vascular Wall Reconstruction
Bačáková, L., Novotná, K., & Pařízek, M. (2014) · Physiological Research · doi:10.33549/physiolres.932644 · Cited by 132
Biomaterial–Related Cell Microenvironment in Tissue Engineering and Regenerative Medicine
Gao, J., Yu, X., Wang, X., He, Y., & Ding, J. (2022) · Engineering · doi:10.1016/j.eng.2021.11.025 · Cited by 136
Solution of the nonlinear elasticity imaging inverse problem: the compressible case
Gokhale, N. H., Barbone, P. E., & Oberai, A. A. (2008) · Inverse Problems · doi:10.1088/0266-5611/24/4/045010 · Cited by 131
Mucin-Inspired Thermoresponsive Synthetic Hydrogels Induce Stasis in Human Pluripotent Stem Cells and Human Embryos
Cantón, I., Warren, N. J., Chahal, A. S., Amps, K., Wood, A., Weightman, R., Wang, E., Moore, H. D. M., & Armes, S. P. (2016) · ACS Central Science · doi:10.1021/acscentsci.5b00370 · Cited by 131
Bio-functionalized silk hydrogel microfluidic systems
Zhao, S., Chen, Y. G., Partlow, B. P., Golding, A. S., Tseng, P., Coburn, J. M., Applegate, M. B., Moreau, J. E., Omenetto, F. G., & Kaplan, D. L. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.03.041 · Cited by 129
3D Printing of Silk Fibroin for Biomedical Applications
Wang, Q., Han, G., Yan, S., & Zhang, Q. (2019) · Materials · doi:10.3390/ma12030504 · Cited by 131
Biofabrication methods for reconstructing extracellular matrix mimetics
Aazmi, A., Zhang, D., Mazzaglia, C., Yu, M., Wang, Z., Yang, H., Huang, Y. Y. S., & Ma, L. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.08.018 · Cited by 142
Correlations between structure, material properties and bioproperties in self-assembled β-hairpin peptide hydrogels
Hule, R. A., Nagarkar, R. P., Altunbaş, A., Ramay, H. R., Branco, M. C., Schneider, J. P., & Pochan, D. J. (2008) · Faraday Discussions · doi:10.1039/b717616c · Cited by 131
Sculpting Organs: Mechanical Regulation of Tissue Development
Nelson, C. M., & Gleghorn, J. P. (2012) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071811-150043 · Cited by 130
Three-dimensional microenvironments modulate fibroblast signaling responses☆
Green, J. B. A., & Yamada, K. M. (2007) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2007.08.005 · Cited by 130
Cell-Imprinted Substrates Direct the Fate of Stem Cells
Mahmoudi, M., Bonakdar, S., Shokrgozar, M. A., Aghaverdi, H., Hartmann, R., Pick, A., Witte, G., & Parak, W. J. (2013) · ACS Nano · doi:10.1021/nn403844q · Cited by 131
Liquid drops attract or repel by the inverted Cheerios effect
Karpitschka, S., Pandey, A., Lubbers, L. A., Weijs, J. H., Botto, L., Das, S., Andreotti, B., & Snoeijer, J. H. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1601411113 · Cited by 130
Mechanobiology of annulus fibrosus and nucleus pulposus cells in intervertebral discs
Molladavoodi, S., McMorran, J. G., & Gregory, D. E. (2019) · Cell and Tissue Research · doi:10.1007/s00441-019-03136-1 · Cited by 134
Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion
Wang, S., Matsumoto, K., Lish, S. R., Cartagena‐Rivera, A. X., & Yamada, K. M. (2021) · Cell · doi:10.1016/j.cell.2021.05.015 · Cited by 124
Substrate Stiffness Regulates Cellular Uptake of Nanoparticles
Huang, C., Butler, P. J., Tong, S., Muddana, H. S., Bao, G., & Zhang, S. (2013) · Nano Letters · doi:10.1021/nl400033h · Cited by 130
Controlling the elastic modulus of cellulose nanofibril hydrogels—scaffolds with potential in tissue engineering
Syverud, K., Pettersen, S. R., Draget, K. I., & Chinga‐Carrasco, G. (2014) · Cellulose · doi:10.1007/s10570-014-0470-5 · Cited by 136
Absence of Filamin A Prevents Cells from Responding to Stiffness Gradients on Gels Coated with Collagen but not Fibronectin
Byfield, F. J., Wen, Q., Levental, I., Nordstrom, K., Arratia, P. E., Miller, R. T., & Janmey, P. A. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2009.03.046 · Cited by 129
Self-citation
Mapping the mechanical stiffness of live cells with the scanning ion conductance microscope
Rheinlaender, J., & Schäffer, T. E. (2013) · Soft Matter · doi:10.1039/c2sm27412d · Cited by 129

Authors: Discher, D. E., Janmey, P., & Wang, Y.-L.
Affiliations: University of Massachusetts Chan Medical School (US)
Corresponding author: Discher, Dennis E.
ORCID: Discher, Dennis E.
Janmey, Paul
Wang, Yu-li
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2005-11-18
Volume: 310
Issue: 5751
Pages: 1139-1143
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1116995
OpenAlex: W2054635074
Cited by: 6,382
References: 82
Citations by year: 2026: 287 · 2025: 269 · 2024: 239 · 2023: 237 · 2022: 318 · 2021: 269 · 2020: 369 · 2019: 389 · 2018: 399 · 2017: 432 · 2016: 379 · 2015: 392 · 2014: 407 · 2013: 369 · 2012: 380
FWCI: 9.07
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Keywords: substrate stiffness, mechanotransduction, cell-matrix adhesion, cell-substrate adhesion, soft agar, cancer cell detection, fibroblasts, muscle cells, neurons, actomyosin cytoskeleton, adhesion complexes, cell contractility, tissue stiffness, cell differentiation, tissue regeneration
MeSH terms: Biomechanical Phenomena, Cell Adhesion, Cell Communication, Cell Physiological Phenomena, Cytoskeleton, Elasticity, Humans, Muscle Contraction, Organogenesis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Tissue Cells Feel and Respond to the Stiffness of Their Substrate

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks