Cited by 6,382Cellular Mechanics and InteractionsForce Microscopy Techniques and ApplicationsHydrogels: synthesis, properties, applications

The DOI matches its Crossref record; 82 of the 82 references it cites were found in a registry or an index.

Tissue Cells Feel and Respond to the Stiffness of Their Substrate

Discher, Dennis E., Janmey, Paul, Wang, Yu-li·2005·Science

DOI 10.1126/science.1116995

Open this paper in CoChat — free account

Abstract

Normal tissue cells are generally not viable when suspended in a fluid and are therefore said to be anchorage dependent. Such cells must adhere to a solid, but a solid can be as rigid as glass or softer than a baby's skin. The behavior of some cells on soft materials is characteristic of important phenotypes; for example, cell growth on soft agar gels is used to identify cancer cells. However, an understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels (or to other cells) with which elasticity can be tuned to approximate that of tissues. Key roles in molecular pathways are played by adhesion complexes and the actinmyosin cytoskeleton, whose contractile forces are transmitted through transcellular structures. The feedback of local matrix stiffness on cell state likely has important implications for development, differentiation, disease, and regeneration.

Integrity signals

Registry

Crossref · 10.1126/science.1116995

Cites

82 of 82 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

New Perspectives in Cell Adhesion: RGD and Integrins
Ruoslahti, E. I., & Pierschbacher, M. D. (1987) · Science · doi:10.1126/science.2821619 · Cited by 4,875
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Self-citation
Cell locomotion and focal adhesions are regulated by substrate flexibility
Pelham, R. J., & Wang, Y. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13661 · Cited by 3,082
Self-citation
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Exploring and Engineering the Cell Surface Interface
Stevens, M. M., & George, J. H. (2005) · Science · doi:10.1126/science.1106587 · Cited by 2,607
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
Yeung, T., Georges, P. C., Flanagan, L. A., Marg, B., Ortiz, M., Funaki, M., Zahir, N., Ming, W., Weaver, V., & Janmey, P. A. (2005) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20041 · Cited by 2,325
Self-citation
Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
Balaban, N. Q., Schwarz, U. S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., Mahalu, D., Safran, S. A., Bershadsky, A. D., Addadi, L., et al. (2001) · Nature Cell Biology · doi:10.1038/35074532 · Cited by 2,137
Cells lying on a bed of microneedles: An approach to isolate mechanical force
Tan, J. L., Tien, J., Pirone, D. M., Gray, D. S., Bhadriraju, K., & Chen, C. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0235407100 · Cited by 2,002
Myotubes differentiate optimally on substrates with tissue-like stiffness
Engler, A. J., Griffin, M. A., Sen, S., Bönnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200405004 · Cited by 1,743
Self-citation
Nonlinear elasticity in biological gels
Storm, C., Pastore, J. J., MacKintosh, F. C., Lubensky, T. C., & Janmey, P. A. (2005) · Nature · doi:10.1038/nature03521 · Cited by 1,707
Self-citation

Show all 82 references

Research works printed but not linked: 5

D. R. Welch et al., Cancer Res.60, 1552 (2000).
F. J. Alenghat, D. E. Ingber, Sci. STKE119, pe6 (2002).
A. Saez A. Buguin P. Silberzan B. Ladoux Biophys J. in press.
J. P. Trinkaus, J. P. Lentz, Dev. Biol.89, 115 (1964).
W. T. Arthur, N. K. Noren, K. Burridge, Biol. Res.35, 239 (2002).

Sorted by citations

Items 901–1,000 of 1,034

Effect of substrate stiffness on early human embryonic stem cell differentiation
Eroshenko, N., Ramachandran, R., Yadavalli, V. K., & Rao, R. R. (2013) · Journal of Biological Engineering · doi:10.1186/1754-1611-7-7 · Cited by 115
3D Bioprinting Methods and Techniques: Applications on Artificial Blood Vessel Fabrication.
Papaioannou, T. G., Manolesou, D. G., Dimakakos, E. P., Tsoucalas, G., Vavuranakis, M., & Tousoulis, D. M. (2019) · PubMed Central · doi:10.6515/acs.201905_35(3).20181115a · Cited by 115
Advanced Strategies for Articular Cartilage Defect Repair
Matsiko, A., Levingstone, T. J., & O’Brien, F. J. (2013) · Materials · doi:10.3390/ma6020637 · Cited by 123
Filamin A Is Essential for Active Cell Stiffening but not Passive Stiffening under External Force
Kasza, K. E., Nakamura, F., Hu, S., Kollmannsberger, P., Bonakdar, N., Fabry, B., Stossel, T. P., Wang, N., & Weitz, D. A. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2009.02.035 · Cited by 115
Molecular Engineering of Cellular Environments: Cell Adhesion to Nano‐Digital Surfaces
Spatz, J. P., & Geiger, B. (2007) · Methods in cell biology · doi:10.1016/s0091-679x(07)83005-6 · Cited by 114
Mimicking dynamic in vivo environments with stimuli-responsive materials for cell culture
Kim, J., & Hayward, R. C. (2012) · Trends in biotechnology · doi:10.1016/j.tibtech.2012.04.003 · Cited by 114
Substrate stiffness regulates B‐cell activation, proliferation, class switch, and T‐cell‐independent antibody responses in vivo
Zeng, Y., Yi, J., Wan, Z., Liu, K., Song, P., Chau, A., Wang, F., Chang, Z., Han, W., Zheng, W., et al. (2015) · European Journal of Immunology · doi:10.1002/eji.201444777 · Cited by 116
Hydroxyapatite-hybridized chitosan/chitin whisker bionanocomposite fibers for bone tissue engineering applications
Pangon, A., Saesoo, S., Saengkrit, N., Ruktanonchai, U., & Intasanta, V. (2016) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2016.02.053 · Cited by 112
Multiscale model predicts increasing focal adhesion size with decreasing stiffness in fibrous matrices
Cao, X. D., Ban, E., Baker, B. M., Lin, Y., Burdick, J. A., Chen, C. S., & Shenoy, V. B. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1620486114 · Cited by 109
Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes
Buxboim, A., Irianto, J., Swift, J., Athirasala, A., Shin, J., Rehfeldt, F., & Discher, D. E. (2017) · Molecular Biology of the Cell · doi:10.1091/mbc.e17-06-0393 · Cited by 114
Self-citation
Mechanical Considerations for Electrospun Nanofibers in Tendon and Ligament Repair
Brennan, D. A., Conte, A. A., Kanski, G. M., Turkula, S., Hu, X., Kleiner, M. T., & Beachley, V. Z. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701277 · Cited by 112
Morphogenetic fields in embryogenesis, regeneration, and cancer: Non-local control of complex patterning
Levin, I. (2012) · Cited by 114
Block Copolymer Photonic Gel for Mechanochromic Sensing
Chan, E. P., Walish, J. J., Thomas, E. L., & Stafford, C. M. (2011) · Advanced Materials · doi:10.1002/adma.201102662 · Cited by 114
Matrix cross-linking–mediated mechanotransduction promotes posttraumatic osteoarthritis
Kim, J.-H., Lee, G., Won, Y., Lee, M.-J., Kwak, J.-S., Chun, C.-H., & Chun, J.-S. (2015) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.1505700112 · Cited by 112
Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesis
Myers, K. A., Applegate, K. T., Danuser, G. M., Fischer, R. S., & Waterman, C. M. (2011) · The Journal of Cell Biology · doi:10.1083/jcb.201006009 · Cited by 113
Three-Dimensional Quantification of Cellular Traction Forces and Mechanosensing of Thin Substrata by Fourier Traction Force Microscopy
del Álamo, J. C., Meili, R., Álvarez-González, B., Alonso‐Latorre, B., Bastounis, E. E., Firtel, R., & Lasheras, J. C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069850 · Cited by 113
Optimisation of UV irradiation as a binding site conserving method for crosslinking collagen-based scaffolds
Davidenko, N., Bax, D. V., Schuster, C. F., Farndale, R. W., Hamaia, S. W., Best, S. M., & Cameron, R. E. (2015) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-015-5627-8 · Cited by 113
Recent developments in the properties and applications of polyelectrolyte multilayers
Jaber, J. A., & Schlenoff, J. B. (2006) · Current Opinion in Colloid & Interface Science · doi:10.1016/j.cocis.2006.09.008 · Cited by 113
Phase Transitions of the Coupled Membrane-Cytoskeleton Modify Cellular Shape
Veksler, A., & Gov, N. S. (2007) · Biophysical Journal · doi:10.1529/biophysj.107.113282 · Cited by 114
Piezoelectric Substrates Promote Neurite Growth in Rat Spinal Cord Neurons
Royo-Gascon, N., Wininger, M., Scheinbeim, J. I., Firestein, B. L., & Craelius, W. (2012) · Annals of Biomedical Engineering · doi:10.1007/s10439-012-0628-y · Cited by 113
Assay to mechanically tune and optically probe fibrillar fibronectin conformations from fully relaxed to breakage
Little, W. C., Smith, M. L., Ebneter, U., & Viola Vogel, B. (2008) · Matrix Biology · doi:10.1016/j.matbio.2008.02.003 · Cited by 114
Photo-Cross-Linked PDMSstar-PEG Hydrogels: Synthesis, Characterization, and Potential Application for Tissue Engineering Scaffolds
Hou, Y., Schoener, C. A., Regan, K. R., Munoz‐Pinto, D. J., Hahn, M. S., & Grunlan, M. A. (2010) · Biomacromolecules · doi:10.1021/bm9012293 · Cited by 113
Lifetime and Strength of Adhesive Molecular Bond Clusters between Elastic Media
Qian, J. W., Wang, J., & Gao, H. (2008) · Langmuir · doi:10.1021/la702401b · Cited by 113
Degradable emulsion-templated scaffolds for tissue engineering from thiol–ene photopolymerisation
Caldwell, S., Johnson, D. W., Didsbury, M. P., Murray, B. A., Wu, J. J., Przyborski, S. A., & Cameron, N. R. (2012) · Soft Matter · doi:10.1039/c2sm26250a · Cited by 113
Surface association and the MreB cytoskeleton regulate pilus production, localization and function in Pseudomonas aeruginosa
Cowles, K. N., & Gitai, Z. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07132.x · Cited by 113
Additive manufacturing of photosensitive hydrogels for tissue engineering applications
Qin, X., Ovsianikov, A., Stampfl, J., & Liska, R. (2014) · BioNanoMaterials · doi:10.1515/bnm-2014-0008 · Cited by 121
Regulation of hepatic stem/progenitor phenotype by microenvironment stiffness in hydrogel models of the human liver stem cell niche
Lozoya, O. A., Wauthier, E., Turner, R., Barbier, C., Prestwich, G. D., Guilak, F., Superfine, R., Lubkin, S. R., & Reid, L. M. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.06.042 · Cited by 114
Polyelectrolyte Multilayers in Tissue Engineering
Detzel, C. J., Larkin, A. L., & Rajagopalan, P. (2011) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2010.0548 · Cited by 112
Three-dimensional neural constructs: a novel platform for neurophysiological investigation
Irons, H. R., Cullen, D. K., Shapiro, N. P., Lambert, N. A., Lee, R. H., & LaPlaca, M. C. (2008) · Journal of Neural Engineering · doi:10.1088/1741-2560/5/3/006 · Cited by 112
Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells
Rehfeldt, F., Brown, A. E., Raab, M., Cai, S., Zajac, A. L., Zemel, A., & Discher, D. E. (2012) · Integrative Biology · doi:10.1039/c2ib00150k · Cited by 112
Self-citation
Nanopatterned Adhesive, Stretchable Hydrogel to Control Ligand Spacing and Regulate Cell Spreading and Migration
Deng, J., Zhao, C., Spatz, J. P., & Wei, Q. (2017) · ACS Nano · doi:10.1021/acsnano.7b03449 · Cited by 112
A photo-modulatable material for probing cellular responses to substrate rigidity
Frey, M. T., & Wang, Y. (2009) · Soft Matter · doi:10.1039/b818104g · Cited by 112
Self-citation
Mechanics of Single Cells: Rheology, Time Dependence, and Fluctuations
Massiera, G., Citters, K. M. V., Biancaniello, P. L., & Crocker, J. C. (2007) · Biophysical Journal · doi:10.1529/biophysj.107.111641 · Cited by 112
Cell Volume Fluctuations in MDCK Monolayers
Zehnder, S. M., Suaris, M., Bellaire, M. M., & Angelini, T. E. (2015) · Biophysical Journal · doi:10.1016/j.bpj.2014.11.1856 · Cited by 111
Biomechanical properties and mechanobiology of the articular chondrocyte
Chen, C., Tambe, D. T., Deng, L., & Yang, L. (2013) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00242.2013 · Cited by 111
Nonlinear Elasticity: From Single Chain to Networks and Gels
Carrillo, J. Y., MacKintosh, F. C., & Dobrynin, A. V. (2013) · Macromolecules · doi:10.1021/ma400478f · Cited by 114
Projection-Based 3D Printing of Cell Patterning Scaffolds with Multiscale Channels
Xue, D., Wang, Y., Zhang, J., Mei, D., Wang, Y., & Chen, S. (2018) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.8b03867 · Cited by 113
Self-citation
Integrating Physical and Molecular Insights on Immune Cell Migration
Moreau, H. D., Piel, M., Voituriez, R., & Lennon‐Duménil, A. (2018) · Trends in Immunology · doi:10.1016/j.it.2018.04.007 · Cited by 111
Nanomechanics of the Endothelial Glycocalyx
Cosgun, Z. C., Fels, B., & Kusche‐Vihrog, K. (2020) · American Journal Of Pathology · doi:10.1016/j.ajpath.2019.07.021 · Cited by 108
Microrheological Characterization of Collagen Systems: From Molecular Solutions to Fibrillar Gels
Shayegan, M., & Forde, N. R. (2013) · PLoS ONE · doi:10.1371/journal.pone.0070590 · Cited by 114
Adhesions that mediate invasion
Gimona, M., & Buccione, R. (2006) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2006.05.003 · Cited by 111
Fibroin/collagen hybrid hydrogels with crosslinking method: Preparation, properties, and cytocompatibility
Lv, Q., Hu, K., Feng, Q., & Cui, F. (2007) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31366 · Cited by 111
Nano-topography sensing by osteoclasts
Geblinger, D., Addadi, L., & Geiger, B. (2010) · Journal of Cell Science · doi:10.1242/jcs.060954 · Cited by 117
Extracellular matrix rigidity modulates neuroblastoma cell differentiation and N-myc expression
Lam, W. A., Cao, L., Umesh, V., Keung, A. J., Sen, S., & Kumar, S. (2010) · Molecular Cancer · doi:10.1186/1476-4598-9-35 · Cited by 111
In situ mechanotransduction via vinculin regulates stem cell differentiation
Holle, A. W., Tang, X., Vijayraghavan, D. S., Vincent, L. G., Fuhrmann, A., Choi, Y. S., del Álamo, J. C., & Engler, A. J. (2013) · Stem Cells · doi:10.1002/stem.1490 · Cited by 114
Mechanical biocompatibility of highly deformable biomedical materials
Mazza, E., & Ehret, A. E. (2015) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2015.03.023 · Cited by 115
Cellular contractility and extracellular matrix stiffness regulate matrix metalloproteinase activity in pancreatic cancer cells
Haage, A., & Schneider, I. C. (2014) · The FASEB Journal · doi:10.1096/fj.13-245613 · Cited by 110
Ultrasoft, highly deformable microgels
Bachman, H. N., Brown, A. C., Clarke, K. C., Dhada, K. S., Douglas, A. M., Hansen, C. E., Herman, E. S., Hyatt, J. S., Kodlekere, P., Meng, Z., et al. (2015) · Soft Matter · doi:10.1039/c5sm00047e · Cited by 110
Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization
Mazza, G., Al‐Akkad, W., Telese, A., Longato, L., Urbani, L., Robinson, B. K., Hall, A. R., Kong, K., Frenguelli, L., Marrone, G., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-05134-1 · Cited by 110
Influence of ionic crosslinkers (Ca2+/Ba2+/Zn2+) on the mechanical and biological properties of 3D Bioplotted Hydrogel Scaffolds
Sarker, M., Izadifar, M., Schreyer, D., & Chen, X. (2018) · Journal of Biomaterials Science, Polymer Edition · doi:10.1080/09205063.2018.1433420 · Cited by 109
3D Printing of Silk Particle-Reinforced Chitosan Hydrogel Structures and Their Properties
Zhang, J., Allardyce, B. J., Rajkhowa, R., Zhao, Y. J., Dilley, R. J., Redmond, S. L., Wang, X., & Liu, X. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00804 · Cited by 114
Bridging the gap in peripheral nerve repair with 3D printed and bioprinted conduits
Dixon, A. R., Jariwala, S. H., Bilis, Z., Loverde, J. R., Pasquina, P. F., & Alvarez, L. M. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.09.010 · Cited by 110
TRPV 4 regulates matrix stiffness and TGF β1‐induced epithelial‐mesenchymal transition
Sharma, S., Goswami, R., Zhang, D. X., & Rahaman, S. O. (2018) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.13972 · Cited by 108
Substrate mechanics controls adipogenesis through YAP phosphorylation by dictating cell spreading
Cruz, J. O. L., Nardone, G., Vrbský, J., Pompeiano, A., Perestrelo, A. R., Capradossi, F., Melajová, K., Filipenský, P., & Forte, G. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.03.009 · Cited by 111
Engineering a clinically-useful matrix for cell therapy
Prestwich, G. D. (2008) · Organogenesis · doi:10.4161/org.6152 · Cited by 110
Coupling biochemistry and mechanics in cell adhesion: a model for inhomogeneous stress fiber contraction
Besser, A., & Schwarz, U. S. (2007) · New Journal of Physics · doi:10.1088/1367-2630/9/11/425 · Cited by 110
Regulation of the fate of human mesenchymal stem cells by mechanical and stereo-topographical cues provided by silicon nanowires
Kuo, S.-W., Lin, H.-I., Ho, J. H., Shih, Y.-R. V., Chen, H.-F., Yen, T., & Lee, O. K. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.03.080 · Cited by 110
Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise
Spiesz, E. M., Thorpe, C. T., Chaudhry, S., Riley, G. P., Birch, H. L., Clegg, P., & Screen, H. R. C. (2015) · Journal of Orthopaedic Research® · doi:10.1002/jor.22879 · Cited by 110
Rheological characterization of an injectable alginate gel system
Larsen, B. E., Bjørnstad, J., Pettersen, E. O., Tønnesen, H. H., & Melvik, J. E. (2015) · BMC Biotechnology · doi:10.1186/s12896-015-0147-7 · Cited by 109
Microfluidics as a functional tool for cell mechanics
Vanapalli, S. A., Duits, M. H. G., & Mugele, F. G. (2009) · Biomicrofluidics · doi:10.1063/1.3067820 · Cited by 111
Dynamic and structural signatures of lamellar actomyosin force generation
Aratyn-Schaus, Y., Oakes, P. W., & Gardel, M. L. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-11-0891 · Cited by 110
Stimuli-Responsive Smart Gels Realized via Modular Protein Design
Grove, T. Ž., Osuji, C. O., Forster, J. D., Dufresne, E. R., & Regan, L. (2010) · Journal of the American Chemical Society · doi:10.1021/ja106619w · Cited by 109
Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry
Gao, X., Fang, J., Xue, B., Fu, L., & Li, H. (2016) · Biomacromolecules · doi:10.1021/acs.biomac.6b00566 · Cited by 116
Electroactive Scaffolds for Neurogenesis and Myogenesis: Graphene‐Based Nanomaterials
Zhang, Z., Klausen, L. H., Chen, M., & Dong, M. (2018) · Small · doi:10.1002/smll.201801983 · Cited by 111
Programmable hydrogels
Wang, Y. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.03.008 · Cited by 109
Self-citation
Steering phase-separated droplets to control fibrillar network evolution of supramolecular peptide hydrogels
Zhou, P., Xing, R., Li, Q., Li, J., Yuan, C., & Yan, X. (2023) · Matter · doi:10.1016/j.matt.2023.03.029 · Cited by 110
Brillouin microscopy
Kabakova, I. V., Zhang, J., Xiang, Y., Caponi, S., Bilenca, A., Guck, J. R., & Scarcelli, G. (2024) · Nature Reviews Methods Primers · doi:10.1038/s43586-023-00286-z · Cited by 116
Staged stromal extracellular 3D matrices differentially regulate breast cancer cell responses through PI3K and beta1-integrins
Castelló-Cros, R., Khan, D. R., Simons, J., Valianou, M., & Cukierman, E. (2009) · BMC Cancer · doi:10.1186/1471-2407-9-94 · Cited by 110
Mechanical Forces Induced by the Transendothelial Migration of Human Neutrophils
Rabodzey, A., Alcaide, P., Luscinskas, F. W., & Ladoux, B. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.119156 · Cited by 109
Hybrid Elastin-like Polypeptide–Polyethylene Glycol (ELP-PEG) Hydrogels with Improved Transparency and Independent Control of Matrix Mechanics and Cell Ligand Density
Wang, H., Cai, L., Paul, A., Enejder, A. M. K., & Heilshorn, S. C. (2014) · Biomacromolecules · doi:10.1021/bm500969d · Cited by 113
The Mouse Follicle Microenvironment Regulates Antrum Formation and Steroid Production: Alterations in Gene Expression Profiles1
West, E. R., Xu, M., Gomberg, M. A., Chow, Y. H., Woodruff, T. K., & Shea, L. D. (2008) · Biology of Reproduction · doi:10.1095/biolreprod.108.071142 · Cited by 109
Stiffness of photocrosslinked RGD‐alginate gels regulates adipose progenitor cell behavior
Chandler, E. M., Berglund, C. M., Lee, J. S., Polacheck, W. J., Gleghorn, J. P., Kirby, B. J., & Fischbach, C. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23079 · Cited by 109
Adhesion of membranes via receptor–ligand complexes: Domain formation, binding cooperativity, and active processes
Weikl, T. R., Asfaw, M., Krobath, H., Różycki, B., & Lipowsky, R. (2009) · Soft Matter · doi:10.1039/b902017a · Cited by 109
Tunable Stress Relaxation Behavior of an Alginate-Polyacrylamide Hydrogel: Comparison with Muscle Tissue
Fitzgerald, M. M., Bootsma, K., Berberich, J. A., & Sparks, J. L. (2015) · Biomacromolecules · doi:10.1021/bm501845j · Cited by 110
A review of decellularized stem cell matrix: a novel cell expansion system for cartilage tissue engineering
Pei, M., Li, J., Shoukry, M. S., & Zhang, Y. (2011) · European Cells and Materials · doi:10.22203/ecm.v022a25 · Cited by 109
Engineered 3D cardiac fibrotic tissue to study fibrotic remodeling
Sadeghi, A. H., Shin, S. R., Deddens, J. C., Fratta, G., Mandla, S., Yazdi, I. K., Prakash, G., Antona, S., Demarchi, D., Buijsrogge, M. P., et al. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201601434 · Cited by 108
Physiologically inspired cardiac scaffolds for tailored in vivo function and heart regeneration
Kaiser, N. J., & Coulombe, K. L. K. (2015) · Biomedical Materials · doi:10.1088/1748-6041/10/3/034003 · Cited by 111
Matrix Stiffness: A Regulator of Cellular Behavior and Tissue Formation
Mason, B. N., Califano, J. P., & Reinhart‐King, C. A. (2011) · Springer eBooks · doi:10.1007/978-1-4614-1080-5_2 · Cited by 107
Engineered extracellular matrices with cleavable crosslinkers for cell expansion and easy cell recovery
Zhang, J., Skardal, A., & Prestwich, G. D. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.08.008 · Cited by 108
Reconstruction of an In Vitro Tissue-Specific Microenvironment to Rejuvenate Synovium-Derived Stem Cells for Cartilage Tissue Engineering
He, F., Chen, X., & Pei, M. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0188 · Cited by 108
Filamin A–β1 Integrin Complex Tunes Epithelial Cell Response to Matrix Tension
Gehler, S., Baldassarre, M., Lad, Y., Leight, J. L., Wozniak, M. A., Riching, K. M., Eliceiri, K. W., Weaver, V. M., Calderwood, D., & Keely, P. J. (2009) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-12-1186 · Cited by 108
Inkjet printing Schwann cells and neuronal analogue NG108-15 cells
Tse, C., Whiteley, R., Yu, T., Stringer, J., MacNeil, S., Haycock, J. W., & Smith, P. J. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/015017 · Cited by 110
Chemical unfolding of protein domains induces shape change in programmed protein hydrogels
Khoury, L. R., & Popa, I. (2019) · Nature Communications · doi:10.1038/s41467-019-13312-0 · Cited by 112
Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture
Kang, S., Lee, J., Huh, Y. S., & Takayama, S. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c00457 · Cited by 109
Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models
Lewns, F. K., Tsigkou, O., Cox, L. R., Wildman, R. D., Grover, L. M., & Poologasundarampillai, G. (2023) · Advanced Materials · doi:10.1002/adma.202301670 · Cited by 111
Microbuckling of fibrin provides a mechanism for cell mechanosensing
Notbohm, J., Lesman, A., Rosakis, P., Tirrell, D. A., & Ravichandran, G. (2015) · Journal of The Royal Society Interface · doi:10.1098/rsif.2015.0320 · Cited by 108
Effect of Substrate Stiffness on Early Mouse Embryo Development
Kolahi, K. S., Donjacour, A. A., Liu, X., Lin, W., Simbulan, R. K., Bloise, E., Maltepe, E., & Rinaudo, P. F. (2012) · PLoS ONE · doi:10.1371/journal.pone.0041717 · Cited by 108
Counterions and Water in Polyelectrolyte Multilayers: A Tale of Two Polycations
Jaber, J. A., & Schlenoff, J. B. (2006) · Langmuir · doi:10.1021/la061839g · Cited by 108
Matrix stiffening sensitizes epithelial cells to EGF and enables the loss of contact inhibition of proliferation
Kim, J., & Asthagiri, A. R. (2011) · Journal of Cell Science · doi:10.1242/jcs.078394 · Cited by 108
The independent roles of mechanical, structural and adhesion characteristics of 3D hydrogels on the regulation of cancer invasion and dissemination
Beck, J. N., Singh, A., Rothenberg, A. R., Elisseeff, J. H., & Ewald, A. J. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.08.077 · Cited by 108
Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation
Katt, M. E., Linville, R. M., Mayo, L. N., Xu, Z. S., & Searson, P. C. (2018) · Fluids and Barriers of the CNS · doi:10.1186/s12987-018-0092-7 · Cited by 102
Modulus-dependent macrophage adhesion and behavior
Irwin, E. F., Saha, K., Rosenbluth, M., Gamble, L. J., Castner, D. G., & Healy, K. E. (2008) · Journal of Biomaterials Science, Polymer Edition · doi:10.1163/156856208786052407 · Cited by 105
Critical areas of cell adhesion on micropatterned surfaces
Yan, C., Sun, J., & Ding, J. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.078 · Cited by 109
In Vivo Evaluation of 3-Dimensional Polycaprolactone Scaffolds for Cartilage Repair in Rabbits
Martínez‐Díaz, S., García‐Giralt, N., Lebourg, M., Gómez-Tejedor, J. A., Vilà-Canet, G., Cáceres, E., Benito, P., Pradas, M. M., Nogués, X., Ribelles, J. L. G., et al. (2010) · The American Journal of Sports Medicine · doi:10.1177/0363546509352448 · Cited by 107
Stiffness of the substrate influences the phenotype of embryonic chicken cardiac myocytes
Bajaj, P., Tang, X., Saif, T. A., & Bashir, R. (2010) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.32951 · Cited by 107
Importance and regulation of adult stem cell migration
de Lucas, B., Pérez, L. M., & Gálvez, B. G. (2017) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.13422 · Cited by 110
Dipeptide Self-Assembled Hydrogels with Tunable Mechanical Properties and Degradability for 3D Bioprinting
Jian, H., Wang, M., Dong, Q., Li, J., Wang, A., Li, X., Ren, P., & Bai, S. (2019) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b13905 · Cited by 107
Assessment of a Bovine Co-culture, Scaffold-Free Method for Growing Meniscus-Shaped Constructs
Aufderheide, A. C., & Athanasiou, K. A. (2007) · Tissue Engineering · doi:10.1089/ten.2006.0291 · Cited by 107
Decoupling Cell and Matrix Mechanics in Engineered Microtissues Using Magnetically Actuated Microcantilevers
Zhao, R., Boudou, T., Wang, W., Chen, C. S., & Reich, D. H. (2013) · Advanced Materials · doi:10.1002/adma.201203585 · Cited by 107
Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching
Zhang, S., Liu, X., Barreto-Ortiz, S. F., Yu, Y., Ginn, B. P., DeSantis, N. A., Hutton, D. L., Grayson, W. L., Cui, F.-Z., Korgel, B. A., et al. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.12.081 · Cited by 106

Authors: Discher, D. E., Janmey, P., & Wang, Y.-L.
Affiliations: University of Massachusetts Chan Medical School (US)
Corresponding author: Discher, Dennis E.
ORCID: Discher, Dennis E.
Janmey, Paul
Wang, Yu-li
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2005-11-18
Volume: 310
Issue: 5751
Pages: 1139-1143
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1116995
OpenAlex: W2054635074
Cited by: 6,382
References: 82
Citations by year: 2026: 287 · 2025: 269 · 2024: 239 · 2023: 237 · 2022: 318 · 2021: 269 · 2020: 369 · 2019: 389 · 2018: 399 · 2017: 432 · 2016: 379 · 2015: 392 · 2014: 407 · 2013: 369 · 2012: 380
FWCI: 9.07
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Keywords: substrate stiffness, mechanotransduction, cell-matrix adhesion, cell-substrate adhesion, soft agar, cancer cell detection, fibroblasts, muscle cells, neurons, actomyosin cytoskeleton, adhesion complexes, cell contractility, tissue stiffness, cell differentiation, tissue regeneration
MeSH terms: Biomechanical Phenomena, Cell Adhesion, Cell Communication, Cell Physiological Phenomena, Cytoskeleton, Elasticity, Humans, Muscle Contraction, Organogenesis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Tissue Cells Feel and Respond to the Stiffness of Their Substrate

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks