Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors
Open accessAbstract
Pediatric and adult kidney tumors differ: Understanding tumor origins and the similarities and differences between organ-specific cancers is important for determining treatment options. Young et al. generated more than 72,000 single-cell transcriptomes from healthy and cancerous human kidneys. From these data, they determined that Wilms tumor, a pediatric kidney cancer, originates from aberrant fetal cells, whereas adult kidney cancers are likely derived from a specific subtype of proximal convoluted tubular cell. Science , this issue p. 594
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Solid-tissue experiments are frequently highly contaminated with red blood cells and red cell lysis is used to prepare the samples.
“For example, solid-tissue experiments are frequently highly contaminated with red blood cells and red cell lysis is used to prepare the samples [15].”
From SoupX removes ambient RNA contamination from droplet-based single-cell RNA sequencing data (2020) · cites this paper as [15]
The paper’s tissue-processing description (Abstract; page not provided) says kidney samples were made into viable single-cell solutions, but does not mention red-blood-cell contamination or red-cell lysis. Searches for red blood cells, erythrocyte lysis, and lysis buffer found no passage addressing either part of the claim. (LLM, conf=0.72)
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- Authors
- Young, M. D., Mitchell, T. J., Vieira Braga, F. A., Tran, M. G. B., Stewart, B. J., Ferdinand, J. R., Collord, G., Botting, R. A., Popescu, D.-M., Loudon, K. W., Vento-Tormo, R., Stephenson, E., Cagan, A., Farndon, S. J., Del Castillo Velasco-Herrera, M., Guzzo, C., Richoz, N., Mamanova, L., Aho, T., Armitage, J. N., Riddick, A. C. P., Mushtaq, I., Farrell, S., Rampling, D., Nicholson, J., Filby, A., Burge, J., Lisgo, S., Maxwell, P. H., Lindsay, S., Warren, A. Y., Stewart, G. D., Sebire, N., Coleman, N., Haniffa, M., Teichmann, S. A., Clatworthy, M., & Behjati, S.
- Affiliations
- Wellcome Sanger Institute (GB)
- University of Cambridge (GB)
- Wellcome Sanger Institute, Hinxton CB10 1SA, UK.
- The Royal Free Hospital (GB)
- MRC Laboratory of Molecular Biology (GB)
- Newcastle University (GB)
- Great Ormond Street Hospital (GB)
- Cambridge University Hospitals NHS Foundation Trust (GB)
- Royal Victoria Infirmary (GB)
- Corresponding author
- Young, Matthew D.
- Mitchell, Thomas J.
- Haniffa, Muzlifah
- Teichmann, Sarah A.
- Clatworthy, Menna
- Behjati, Sam
- ORCID
- Young, Matthew D.
- Mitchell, Thomas J.
- Vieira Braga, Felipe A.
- Tran, Maxine G. B.
- Stewart, Benjamin J.
- Ferdinand, John R.
- Collord, Grace
- Botting, Rachel A.
- Loudon, Kevin W.
- Stephenson, Emily
- Cagan, Alex
- Farndon, Sarah J.
- Del Castillo Velasco-Herrera, Martin
- Guzzo, Charlotte
- Richoz, Nathan
- Mamanova, Lira
- Aho, Tevita
- Mushtaq, Imran
- Rampling, Dyanne
- Nicholson, James
- Filby, Andrew
- Lisgo, Steven
- Maxwell, Patrick H.
- Lindsay, Susan
- Warren, Anne Y.
- Stewart, Grant D.
- Sebire, Neil
- Coleman, Nicholas
- Haniffa, Muzlifah
- Teichmann, Sarah A.
- Clatworthy, Menna
- Behjati, Sam
- Venue
- Science
- Published
- 2018-08-10
- Volume
- 361
- Issue
- 6402
- Pages
- 594-599
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC6104812
- OpenAlex
- W2887326710
- Cited by
- 762
- References
- 5
- Citations by year
- 2026: 57 · 2025: 95 · 2024: 107 · 2023: 126 · 2022: 105 · 2021: 110 · 2020: 102 · 2019: 51 · 2018: 6 · 2017: 1
- FWCI
- 26.06
- Type
- journal article
- Language
- en
- Open access
- green
- pure.amsterdamumc.nl
- Licence
- other-oa
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Apollo (University of Cambridge) (submittedVersion)
- Apollo
- Funders
- Human Frontier Science Program (Long-Term Fellowship)
- Wellcome Trust (110104/Z/15/Z, WT107931/Z/15/Z, Studentship, 099175/Z/12/Z)
- Cancer Research UK (Core Infrastructure)
- Cambridge Biomedical Research Campus (Core Infrastructure, MR/N024907/1)
- Kidney Cancer UK (Nick Maude Memorial Fund)
- Facing up 2 Kidney Cancer
- St Baldrick’s Foundation (Robert J Arceci International Award)
- Lister Institute of Preventive Medicine
- NIHR and Newcastle-Biomedical Research Centre
- Arthritis Research UK (21777)
- EMBO (Long-Term Fellowship)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Renal cell carcinoma treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
- Keywords
- kidney cancer, Wilms tumor, renal cell carcinoma, single-cell transcriptomics, kidney development, fetal kidney, proximal convoluted tubule, tumor microenvironment, VEGF signaling, tumor cell of origin, childhood cancer
- MeSH terms
- Adult, Carcinoma, Renal Cell, Child, Humans, Kidney, Kidney Neoplasms, Wilms Tumor, Genetic Variation, Single-Cell Analysis, Transcriptome
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