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Cited by 285Neuroinflammation and Neurodegeneration MechanismsSingle-cell and spatial transcriptomicsMultiple Sclerosis Research Studies

Barcoded viral tracing of single-cell interactions in central nervous system inflammation

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Clark, Iain C., Gutiérrez-Vázquez, Cristina, Wheeler, Michael A., Li, Zhaorong, Rothhammer, Veit, Linnerbauer, Mathias, Sanmarco, Liliana M., Guo, Lydia +23 more·2021·Science
DOI 10.1126/science.abf1230PMC PMC8157482
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Abstract

Single-cell analysis of CNS interactions: Despite their importance in the physiology and pathology of the central nervous system (CNS), few methods are available for the unbiased, systematic investigation of cell-to-cell interactions at single-cell resolution. Clark et al. developed RABID-seq, a method that combines barcoded viral tracing with single-cell RNA sequencing (see the Perspective by Silvin and Ginhoux). RABID-seq identified the axon guidance molecules Sema4D-PlexinB2 and EphrinB3-EphB3 as mediators of microglia-astrocyte interactions that promote CNS pathology in experimental autoimmune encephalomyelitis and, potentially, multiple sclerosis. These studies also identified candidate therapeutic molecules for the modulation of microglia-astrocyte interactions in multiple sclerosis. Science , this issue p. eabf1230 ; see also p. 342

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Authors
Clark, I. C., Gutiérrez-Vázquez, C., Wheeler, M. A., Li, Z., Rothhammer, V., Linnerbauer, M., Sanmarco, L. M., Guo, L., Blain, M., Zandee, S. E. J., Chao, C.-C., Batterman, K. V., Schwabenland, M., Lotfy, P., Tejeda-Velarde, A., Hewson, P., Manganeli Polonio, C., Shultis, M. W., Salem, Y., Tjon, E. C., Fonseca-Castro, P. H., Borucki, D. M., Alves de Lima, K., Plasencia, A., Abate, A. R., Rosene, D. L., Hodgetts, K. J., Prinz, M., Antel, J. P., Prat, A., & Quintana, F. J.
Affiliations
  • QB3 (US)
  • Ann Romney Center for Neurologic Diseases, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Broad Institute (US)
  • Brigham and Women's Hospital (US)
  • Montreal Neurological Institute and Hospital (CA)
  • Neuroimmunology Research Laboratory, Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC H2X 0A9, Canada.
  • Boston University (US)
  • University of Freiburg (DE)
  • Centre Hospitalier de l’Université de Montréal (CA)
Corresponding author
  • Clark, Iain C.
  • Wheeler, Michael A.
  • Quintana, Francisco J.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2021-04-23
Volume
372
Issue
6540
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.abf1230
PMC
PMC8157482
OpenAlex
W3156792714
Cited by
285
References
23
Citations by year
2026: 39 · 2025: 64 · 2024: 51 · 2023: 52 · 2022: 55 · 2021: 24
FWCI
18.82
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institutes of Health (RG4111A1, JF2161-A-5, 1K99NS114111, F32NS101790, T32CA207201, 5T32AG000222)
  • American Cancer Society (PA-1604-08459)
  • National Multiple Sclerosis Society (RSG-14-198-01-LIB)
  • ASCRS Research Foundation (DFG RO4866 1/1)
  • Fonds de Recherche du Québec - Santé (104-2917-I-564)
  • Deutsche Forschungsgemeinschaft (CRC/TRR167)
  • São Paulo Research Foundation (2019/13731-0, R21AG061678)
  • National Cancer Institute, Cairo University (2P30CA006516-48)
  • Ministry of Science and Technology, Government of the People’s Republic of Bangladesh (F30AG063463)
  • This (NS102807, ES02530, ES029136, AI126880)
  • Alfonso Mart Escudero Foundation (ALTF 610-2017)
  • Mallinkrodt Pharmaceuticals (A219074)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
Keywords
astrocyte-microglia interaction, cell-cell interactions, experimental autoimmune encephalomyelitis, multiple sclerosis, axon guidance molecules, Sema4D, ephrin-B3, EphB3, neuroinflammation, pro-inflammatory response
MeSH terms
RNA-Seq, Animals, Astrocytes, Brain, Cell Communication, Central Nervous System, Encephalomyelitis, Autoimmune, Experimental, Humans, Male, Mice, Inbred C57BL, Mitochondria, Multiple Sclerosis, Nerve Tissue Proteins, Herpesvirus 1, Suid, Receptors, Cell Surface, T-Lymphocytes, Signal Transduction, Antigens, CD, NF-kappa B, Reactive Oxygen Species, Microglia, Receptor, EphB3, Ephrin-B3, Semaphorins, Mice, TOR Serine-Threonine Kinases, Single-Cell Analysis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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