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Cited by 31

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Nitric Oxide-Mediated Induction of Dispersal in Pseudomonas aeruginosa Biofilms Is Inhibited by Flavohemoglobin Production and Is Enhanced by Imidazole

Zhu, Xinyi, Oh, Hyun-Suk, Ng, Yu Chiu Beverly, Tang, Pei Yi Peggy, Barraud, Nicolas, Rice, Scott A.·2018·Antimicrobial Agents and Chemotherapy
DOI 10.1128/aac.01832-17
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Abstract

The biological signal molecule nitric oxide (NO) was found to induce biofilm dispersal across a range of bacterial species, which led to its consideration for therapeutic strategies to treat biofilms and biofilm-related infections. However, biofilms are often not completely dispersed after exposure to NO. To better understand this phenomenon, we investigated the response of Pseudomonas aeruginosa biofilm cells to successive NO treatments. When biofilms were first pretreated with a low, noneffective dose of NO, a second dose of the signal molecule at a concentration usually capable of inducing dispersal did not have any effect. Amperometric analysis revealed that pretreated P. aeruginosa cells had enhanced NO-scavenging activity, and this effect was associated with the production of the flavohemoglobin Fhp. Further, quantitative real-time reverse transcription-PCR (qRT-PCR) analysis showed that fhp expression increased by over 100-fold in NO-pretreated biofilms compared to untreated biofilms. Biofilms of mutant strains harboring mutations in fhp or fhpR , encoding a NO-responsive regulator of fhp , were not affected in their dispersal response after the initial pretreatment with NO. Overall, these results suggest that FhpR can sense NO to trigger production of the flavohemoglobin Fhp and inhibit subsequent dispersal responses to NO. Finally, the addition of imidazole, which can inhibit the NO dioxygenase activity of flavohemoglobin, attenuated the prevention of dispersal after NO pretreatment and improved the dispersal response in older, starved biofilms. This study clarifies the underlying mechanisms of impaired dispersal induced by repeated NO treatments and offers a new perspective for improving the use of NO in biofilm control strategies.

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Metadata

Authors
Zhu, X., Oh, H.-S., Ng, Y. C. B., Tang, P. Y. P., Barraud, N., & Rice, S. A.
Affiliations
  • Singapore Centre for Environmental Life Sciences Engineering, Interdisciplinary Graduate School, Nanyang Technological University, Singapore
  • Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore
  • School of Biological Sciences, Nanyang Technological University, Singapore
  • Genetics of Biofilms Unit, Institut Pasteur, Paris, France
ORCID
Rice, Scott A.
Venue
Antimicrobial Agents and Chemotherapy
Publisher
American Society for Microbiology
Published
2018-03
Volume
62
Issue
3
ISSN
  • 0066-4804
  • 1098-6596
DOI
10.1128/aac.01832-17
Cited by
31
References
7
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Funders
  • Environment and Water Research Initiative (1301-IRIS-57)
  • Investissment d'Avenir Program (ANR-10-LABX-62-IBEID)
  • Ministry of Education - Singapore
  • National Research Foundation Singapore

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CoChat. (2026). Citation check: Nitric Oxide-Mediated Induction of Dispersal in Pseudomonas aeruginosa Biofilms Is Inhibited by Flavohemoglobin Production and Is Enhanced by Imidazole. https://app.cochat.ai/papers/10.1128/aac.01832-17
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Zhu, X., Oh, H.-S., Ng, Y. C. B., Tang, P. Y. P., Barraud, N., & Rice, S. A. (2018). Nitric Oxide-Mediated Induction of Dispersal in Pseudomonas aeruginosa Biofilms Is Inhibited by Flavohemoglobin Production and Is Enhanced by Imidazole. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.01832-17

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