Adherence of Candida albicans to Silicone Induces Immediate Enhanced Tolerance to Fluconazole
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Wild-type and efflux pump-deficient cells of Candida albicans adhering to silicone were compared with planktonic cells by flow cytometry for their relative resistance to fluconazole (FCZ). Flow cytometry data on cells carrying a fusion of green fluorescent protein to efflux pump promoters confirmed that enhanced tolerance of attached cells to FCZ was due in part to increased expression of CaMDR1 and CDR1 promoters. Within 2 h of their attachment to silicone, the adherent cells demonstrated levels of FCZ tolerance shown by cells from 24-h biofilms. Following their mechanical detachment, this subset of cells retained a four- to eightfold increase in tolerance compared with the tolerance of planktonic cells for at least two generations. Enhanced efflux pump tolerance to FCZ appeared to be induced within the initial 15 min of attachment in a subset of cells that were firmly attached to the substrata.
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The surface adherence alone seems enough to intensify the expression of the genes encoding the efflux pumps.
“The surface adherence alone seems enough to intensify the expression of the genes encoding the efflux pumps [140]”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [140]
In planktonic cells, efflux pumps are typically upregulated in response to antifungal drugs; in biofilms, they become upregulated within the first few hours of surface contact and remain upregulated throughout biofilm development, whether or not an antifungal drug is present.
“In planktonic cells, these efflux pumps are typically upregulated in response to antifungal drugs; in biofilms, however, they become upregulated within the first few hours of surface contact and remain upregulated throughout biofilm development, whether or not an antifungal drug is present (119, 126, 133, 140, 156, 205).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [119]
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- Authors
- Mateus, C., Crow, S. A., & Ahearn, D. G.
- Affiliations
- Georgia State University (US)
- Corresponding author
- Mateus, Carolina
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2004-09
- Volume
- 48
- Issue
- 9
- Pages
- 3358-3366
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC514749
- OpenAlex
- W2119761757
- Cited by
- 113
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 1 · 2020: 6 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 6 · 2014: 6 · 2013: 5 · 2012: 3
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Candida albicans, antifungal resistance, biofilm formation, cell adhesion, silicone, efflux pumps, CDR1, promoter expression
- MeSH terms
- Antifungal Agents, Candida albicans, Cell Adhesion, Genotype, Microscopy, Fluorescence, Promoter Regions, Genetic, Silicones, Fluconazole, Chromosomes, Fungal, Gene Expression Regulation, Fungal, DNA Primers, ATP Binding Cassette Transporter, Subfamily B, Biofilms, Reverse Transcriptase Polymerase Chain Reaction, Drug Resistance, Fungal
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