Probiotics Reduce Enterohemorrhagic Escherichia coli O157:H7- and Enteropathogenic E. coli O127:H6-Induced Changes in Polarized T84 Epithelial Cell Monolayers by Reducing Bacterial Adhesion and Cytoskeletal Rearrangements
Open accessAbstract
The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance. These findings demonstrate that probiotics prevent epithelial injury induced by attaching-effacing bacteria.
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The S-layer protein extract of L. helveticus R0052 has been shown to ameliorate the pathogenesis of E coli O157:H7.
“The S-layer protein extract of L. helveticus R0052 has been shown to ameliorate the pathogenesis of E coli O157:H7 (Sherman et al., 2005; Johnson-Henry et al., 2007).”
From Lactobacillus helveticus: the proteolytic system (2013) · cites this paper as Sherman et al., 2005
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Probiotics and the gut microbiota in intestinal health and disease
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Dempsey, E., & Corr, S. C. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.840245 · Cited by 579 · see citing works
Anderson, R. C., Cookson, A. L., McNabb, W. C., Park, Z., McCann, M. J., Kelly, W. J., & Roy, N. C. (2010) · BMC Microbiology · doi:10.1186/1471-2180-10-316 · Cited by 510 · see citing works
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Bron, P. A., Kleerebezem, M., Brummer, R.-J., Cani, P. D., Mercenier, A., MacDonald, T. T., Garcia-Ródenas, C. L., & Wells, J. M. (2017) · British Journal Of Nutrition · doi:10.1017/s0007114516004037 · Cited by 499 · see citing works
“Lactobacillus acidophilus LB((57)) and Lactobacillus helveticus R0052((58)) have been shown to antagonise the cytoskeleton rearrangements caused by EPEC in vitro.”
BackgroundGareau, M. G., Jury, J., MacQueen, G., Sherman, P. M., & Perdue, M. H. (2007) · Gut · doi:10.1136/gut.2006.117176 · Cited by 472 · see citing works
Self-citationVanderpool, C., Yan, F., & Polk, B. D. (2008) · Inflammatory Bowel Diseases · doi:10.1002/ibd.20525 · Cited by 340 · see citing works
Lactobacillus surface layer proteins: Structure, function and applications
Hynönen, U., & Palva, A. (2013) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-4962-2 · Cited by 283 · see citing works
Johnson-Henry, K. C., Hagen, K. E., Gordonpour, M., Tompkins, T. A., & Sherman, P. M. (2007) · Cellular Microbiology · doi:10.1111/j.1462-5822.2006.00791.x · Cited by 274 · see citing works
Self-citationReview: The use of direct fed microbials to mitigate pathogens and enhance production in cattle
McAllister, T. A., Beauchemin, K. A., Alazzeh, A. Y., Baah, J., Teather, R. M., & Stanford, K. (2011) · Canadian Journal of Animal Science · doi:10.4141/cjas10047 · Cited by 251 · see citing works
Lactobacillus helveticus: the proteolytic system
Griffiths, M. W., & Tellez, A. M. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00030 · Cited by 241 · see citing works
“Another study conducted on the effect of L. helveticus R0052 to inhibit E coli O157:H7 attachment (Johnson-Henry et al., 2007), tested the influence of different media on the S-layer protein.”
BackgroundInfluentialUnraveling Mechanisms of Action of Probiotics
Sherman, P. M., Ossa, J. C., & Johnson‐Henry, K. (2009) · Nutrition in Clinical Practice · doi:10.1177/0884533608329231 · Cited by 230 · see citing works
Self-citationArseneault-Bréard, J., Rondeau, I., Gilbert, K., Girard, S.-A., Tompkins, T. A., Godbout, R., & Rousseau, G. (2012) · British Journal of Nutrition · doi:10.1017/s0007114511005137 · Cited by 205 · see citing works
Self-citationGirard, S.-A., Bah, T. M., Kaloustian, S., Lada-Moldovan, L., Rondeau, I., Tompkins, T. A., Godbout, R., & Rousseau, G. (2009) · British Journal of Nutrition · doi:10.1017/s0007114509990766 · Cited by 70 · see citing works
Self-citation
- Authors
- Sherman, P. M., Johnson-Henry, K. C., Yeung, H. P., Ngo, P. S. C., Goulet, J., & Tompkins, T. A.
- Affiliations
- University of Toronto (CA)
- Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario
- Université Laval (CA)
- Lallemand (Canada) (CA)
- Corresponding author
- Sherman, Philip M.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2005-08
- Volume
- 73
- Issue
- 8
- Pages
- 5183-5188
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC1201237
- OpenAlex
- W2112364955
- Cited by
- 246
- References
- 0
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 6 · 2023: 5 · 2022: 18 · 2021: 11 · 2020: 13 · 2019: 14 · 2018: 12 · 2017: 9 · 2016: 9 · 2015: 13 · 2014: 10 · 2013: 17 · 2012: 21
- FWCI
- 4.42
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- probiotics, lactic acid bacteria, Escherichia coli O157:H7, epithelial damage, bacterial adhesion, cytoskeletal rearrangement, transepithelial electrical resistance, intestinal barrier
- MeSH terms
- Actinin, Bacterial Adhesion, Cytoskeleton, Epithelial Cells, Escherichia coli Infections, Humans, Lactobacillus acidophilus, Escherichia coli O157, Probiotics
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