Lactobacillus helveticus and Bifidobacterium longum taken in combination reduce the apoptosis propensity in the limbic system after myocardial infarction in a rat model
Open accessAbstract
Myocardial infarction (MI) stimulates the release of pro-inflammatory substances that induce apoptosis in the limbic system. Pro-inflammatory cytokines are considered as the root cause of apoptosis, although the mechanism is not fully explained and/or understood at this time. In addition, depression may induce gastrointestinal perturbations that maintain the elevated levels of pro-inflammatory cytokines. It has been shown that some specific probiotic formulations may reduce gastrointestinal problems induced by stress and the pro/anti-inflammatory cytokine ratio. Therefore, we hypothesised that probiotics, when given prophylactically, may diminish the apoptosis propensity in the limbic system following a MI. Male adult Sprague–Dawley rats were given probiotics (Lactobacillus helveticus and Bifidobacterium longum in combination) or placebo in their drinking-water for four consecutive weeks. A MI was then induced in the rats by occluding the left anterior coronary artery for 40 min. Rats were killed following a 72 h reperfusion period. Infarct size was not different in the two groups. Bax/Bcl-2 (pro-apoptotic/anti-apoptotic) ratio and caspase-3 (pro-apoptotic) activity were reduced in the amygdala (lateral and medial), as well as in the dentate gyrus in the probiotics group when compared with the placebo. Akt activity (anti-apoptotic) was increased in these same three regions. No significant difference was observed in Ca1 and Ca3 for the different markers measured. In conclusion, the probiotics L. helveticus and B. longum, given in combination as preventive therapy, reduced the predisposition of apoptosis found in different cerebral regions following a MI.
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In laboratory rats subjected to experimental myocardial infarction, the same probiotic combination reduced the increase in intestinal permeability and stress-induced cerebral apoptosis.
“When administered to laboratory rats subjected to experimental myocardial infarction, the same probiotic combination reduced the increase in intestinal permeability127 and stress-induced cerebral apoptosis128 found in animals that underwent infarction without probiotic pretreatment.”
From The Gut Microbiome and the Brain (2014) · cites this paper as [128]
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“Probiotics also have extra-intestinal effects, such as prevention of myocardial infarctinduced apoptosis in the limbic region of the brain.(39) To determine the mechanism of action of microbe-induced memory deficits, the hippocampus was studied.”
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- Authors
- Girard, S.-A., Bah, T. M., Kaloustian, S., Lada-Moldovan, L., Rondeau, I., Tompkins, T. A., Godbout, R., & Rousseau, G.
- Affiliations
- Hôpital du Sacré-Cœur de Montréal (CA)
- Lallemand (Canada) (CA)
- Corresponding author
- Rousseau, Guy
- Venue
- British Journal of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 2009-06-29
- Volume
- 102
- Issue
- 10
- Pages
- 1420-1425
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W2061164668
- Cited by
- 70
- References
- 42
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 9 · 2021: 5 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 9 · 2012: 4
- FWCI
- 7.89
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Digital Access to Libraries (submittedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada (250234-07)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Keywords
- Lactobacillus helveticus, Bifidobacterium longum, probiotics, myocardial infarction, apoptosis, limbic system, amygdala, dentate gyrus, Bax/Bcl-2 ratio, caspase-3, Akt, inflammatory cytokines, depression, ischemia-reperfusion injury, gut-brain axis
- MeSH terms
- Animals, Bifidobacterium, Diet, Enzyme Activation, Limbic System, Male, Myocardial Infarction, Nutritional Physiological Phenomena, Rats, Sprague-Dawley, Apoptosis, Proto-Oncogene Proteins c-bcl-2, Probiotics, bcl-2-Associated X Protein, Rats, Lactobacillus helveticus, Caspase 3
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