A G1 Cyclin Is Necessary for Maintenance of Filamentous Growth in Candida albicans
Open accessAbstract
Candida albicans undergoes a dramatic morphological transition in response to various growth conditions. This ability to switch from a yeast form to a hyphal form is required for its pathogenicity. The intractability of Candida to traditional genetic approaches has hampered the study of the molecular mechanism governing this developmental switch. Our approach is to use the more genetically tractable yeast Saccharomyces cerevisiae to yield clues about the molecular control of filamentation for further studies in Candida. G1 cyclins Cln1 and Cln2 have been implicated in the control of morphogenesis in S. cerevisiae. We show that C. albicans CLN1 (CaCLN1) has the same cell cycle-specific expression pattern as CLN1 and CLN2 of S. cerevisiae. To investigate whether G1 cyclins are similarly involved in the regulation of cell morphogenesis during the yeast-to-hypha transition of C. albicans, we mutated CaCLN1. Cacln1/Cacln1 cells were found to be slower than wild-type cells in cell cycle progression. The Cacln1/Cacln1 mutants were also defective in hyphal colony formation on several solid media. Furthermore, while mutant strains developed germ tubes under several hypha-inducing conditions, they were unable to maintain the hyphal growth mode in a synthetic hypha-inducing liquid medium and were deficient in the expression of hypha-specific genes in this medium. Our results suggest that CaCln1 may coordinately regulate hyphal development with signal transduction pathways in response to various environmental cues.
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Three deletion mutants were significantly more fit than the WT parental strain (SN250), including CCN1 and orf19.4471.
“Three were significantly more fit than the WT parental strain (SN250, red, Figure 8), including CCN1, that encodes a G1 cyclin required for hyphal growth maintenance (Loeb et al., 1999) and orf19.4471, an ortholog of Saccharomyces cerevisiae VPS64”
From The evolution of drug resistance in clinical isolates of Candida albicans. (2015) · cites this paper as Loeb et al., 1999
The G1 cyclin Cln3 is required to maintain yeast-form growth, whereas another G1 cyclin, Ccn1, is required for hyphal extension.
“The G1 cyclin Cln3, for example, is required to maintain yeast-form growth, whereas another G1 cyclin, Ccn1, is required for hyphal extension (Loeb et al., 1999; Bachewich and Whiteway, 2005; Chapa y Lazo et al., 2005).”
From Cdc28 provides a molecular link between Hsp90, morphogenesis, and cell cycle progression in<i>Candida albicans</i> (2012) · cites this paper as Loeb et al., 1999
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- Authors
- Loeb, J. D. J., Sepulveda-Becerra, M., Hazan, I., & Liu, H.
- Affiliations
- University of California, Irvine (US)
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1999-06-01
- Volume
- 19
- Issue
- 6
- Pages
- 4019-4027
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC104361
- OpenAlex
- W2222283485
- Cited by
- 105
- References
- 65
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 4
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (GM 55155, R01GM055155)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida albicans, G1 cyclins, CLN1, CLN2, hyphal morphogenesis, yeast-to-hypha transition, filamentous growth, germ tube formation, cell cycle progression, signal transduction, pathogenicity
- MeSH terms
- Actins, Candida albicans, Cell Cycle, Fungal Proteins, Genetic Complementation Test, Genotype, Membrane Glycoproteins, Microscopy, Fluorescence, Models, Genetic, Phenotype, Plasmids, Time Factors, Blotting, Northern, Gene Expression Regulation, Fungal, Cyclins, Mutagenesis, Cell Culture Techniques, Arabidopsis Proteins, Cyclin G
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