Dual Activators of the Sterol Biosynthetic Pathway of Saccharomyces cerevisiae : Similar Activation/Regulatory Domains but Different Response Mechanisms

Davies, Brandon S. J.; Wang, Helen S.; Rine, Jasper

doi:10.1128/mcb.25.16.7375-7385.2005

Cited by 138Fungal and yeast genetics researchGenomics and Chromatin DynamicsBacterial Genetics and Biotechnology

Dual Activators of the Sterol Biosynthetic Pathway of Saccharomyces cerevisiae : Similar Activation/Regulatory Domains but Different Response Mechanisms

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Davies, Brandon S. J., Wang, Helen S., Rine, Jasper·2005·Molecular and Cellular Biology

DOI 10.1128/mcb.25.16.7375-7385.2005PMC PMC1190251

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Abstract

Genes encoding biosynthetic enzymes that make ergosterol, the major fungal membrane sterol, are regulated, in part, at the transcriptional level. Two transcription factors, Upc2p and Ecm22p, bind to the promoters of most ergosterol biosynthetic (ERG) genes, including ERG2 and ERG3, and activate these genes upon sterol depletion. We have identified the transcriptional activation domains of Upc2p and Ecm22p and found that UPC2-1, a mutation that allows cells to take up sterols aerobically, increased the potency of the activation domain. The equivalent mutation in ECM22 also greatly enhanced transcriptional activation. The C-terminal regions of Upc2p and Ecm22p, which contained activation domains, also conferred regulation in response to sterol levels. Hence, the activation and regulatory domains of these proteins overlapped. However, the two proteins differed markedly in how they respond to an increased need for sterols. Upon inducing conditions, Upc2p levels increased, and chromatin immunoprecipitation experiments revealed more Upc2p at promoters even when the activation/regulatory domains were tethered to a different DNA-binding domain. However, induction resulted in decreased Ecm22p levels and a corresponding decrease in the amount of Ecm22p bound to promoters. Thus, these two activators differ in their contributions to the regulation of their targets.

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G898D UPC2A behaves as a hyperactive transcriptional activator in C. glabrata, as previously seen for G888D Upc2 in S. cerevisiae.
“This is consistent with G898D UPC2A behaving as a hyperactive transcriptional activator in C. glabrata as has previously been seen for G888D Upc2 in S. cerevisiae [25].”
From The Candida glabrata Upc2A transcription factor is a global regulator of antifungal drug resistance pathways (2021) · cites this paper as [25]
At the same time, the levels of Upc2, and their association to ERG promoters, strongly increase, which finally results in the transcriptional induction of ERG genes.
“At the same time, the levels of Upc2, and their association to ERG promoters, strongly increase, which finally results in the transcriptional induction of ERG genes [68,74].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [74]
Upon low sterol levels, the abundance of Ecm22 protein, and consequently its binding to ERG promoters, decreases in a process that involves its direct physical interaction with the repressor Mot3.
“Upon low sterol levels, the abundance of Ecm22 protein, and consequently its binding to ERG promoters, decreases in a process that involves its direct physical interaction with the repressor Mot3 (Figure 3B) [74,75].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [74]
Upon ergosterol depletion, the dissociation of the sterol ligand causes a conformational change that exposes Upc2 NLS, causing its translocation to the nucleus, where it activates the transcription of SRE/AR1c-containing genes including most ERG genes, sterol uptake genes (AUS1, PDR11), the DAN/TIR genes, and members of the PAU gene family.
“Upon ergosterol depletion, the dissociation of the sterol ligand causes a conformational change that exposes Upc2 NLS, causing its translocation to the nucleus, where it activates the transcription of SRE/AR1c-containing genes including most ERG genes, sterol uptake genes (AUS1, PDR11), the DAN/TIR genes, which encode nine cell wall mannoproteins, and members of the seripauperin (PAU) gene family”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [74]

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Sikorski, R. S., & Hieter, P. (1989) · Genetics · doi:10.1093/genetics/122.1.19 · Cited by 8,685 · see citing works
A generic protein purification method for protein complex characterization and proteome exploration
Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., & Séraphin, B. (1999) · Nature Biotechnology · doi:10.1038/13732 · Cited by 2,707 · see citing works
Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
James, P. M., Halladay, J. T., & Craig, E. A. (1996) · Genetics · doi:10.1093/genetics/144.4.1425 · Cited by 2,638 · see citing works
Functional Discovery via a Compendium of Expression Profiles
Hughes, T. R., Marton, M. J., Jones, A. R., Roberts, C. J., Stoughton, R. B., Armour, C. D., Bennett, H. A., Coffey, E. M., Dai, H. A., He, Y. D., et al. (2000) · Cell · doi:10.1016/s0092-8674(00)00015-5 · Cited by 2,534 · see citing works
Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
Amberg, D. C., Burke, D. J., & Strathern, J. N. (2005) · Cited by 1,853 · see citing works
Sequencing and comparison of yeast species to identify genes and regulatory elements
Kellis, M., Patterson, N. J., Endrizzi, M. G., Birren, B. W., & Lander, E. S. (2003) · Nature · doi:10.1038/nature01644 · Cited by 1,731 · see citing works
Deletion analysis of GAL4 defines two transcriptional activating segments
Ma, J., & Ptashne, M. (1987) · Cell · doi:10.1016/0092-8674(87)90081-x · Cited by 887 · see citing works
Finding Functional Features in Saccharomyces Genomes by Phylogenetic Footprinting
Cliften, P. F., Sudarsanam, P., Desikan, A., Fulton, L. A., Fulton, B., Majors, J. E., Waterston, R., Cohen, B. A., & Johnston, M. (2003) · Science · doi:10.1126/science.1084337 · Cited by 805 · see citing works
Reduced levels of hsp90 compromise steroid receptor action in vivo
Picard, D., Khursheed, B., Garabedian, M. J., Fortin, M. G., Lindquist, S., & Yamamoto, K. R. (1990) · Nature · doi:10.1038/348166a0 · Cited by 787 · cited 1× · see citing works
“Perhaps a portion of the Upc2p protein interferes with the DNA-binding domain in a nonspecific manner until it is depleted of some pathway intermediate, in a manner somewhat analogous to the way that Hsp90 binds to unliganded glucocorticoid receptors (30).”
Background
Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae
Zinser, E., Sperka-Gottlieb, C. D. M., Fasch, E.-V., Kohlwein, S. D., Paltauf, F., & Daum, G. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.6.2026-2034.1991 · Cited by 705 · see citing works
The Ashbya gossypii Genome as a Tool for Mapping the Ancient Saccharomyces cerevisiae Genome
Dietrich, F. S., Voegeli, S., Brachat, S., Lerch, A., Gates, K., Steiner, S., Mohr, C., Pöhlmann, R., Luedi, P. P., Choi, S., et al. (2004) · Science · doi:10.1126/science.1095781 · Cited by 631 · see citing works
Separation of DNA Binding from the Transcription-Activating Function of a Eukaryotic Regulatory Protein
Keegan, L. P., Gill, G., & Ptashne, M. (1986) · Science · doi:10.1126/science.3080805 · Cited by 612 · see citing works
Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium
Andreasen, A. A., & Stier, T. J. B. (1953) · Journal of Cellular and Comparative Physiology · doi:10.1002/jcp.1030410103 · Cited by 555 · see citing works
Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme
Kuras, L., & Struhl, K. (1999) · Nature · doi:10.1038/21239 · Cited by 450 · see citing works
Direct Binding of Cholesterol to the Purified Membrane Region of SCAP
Radhakrishnan, A., Sun, L.-P., Kwon, H. J., Brown, M. S., & Goldstein, J. L. (2004) · Molecular Cell · doi:10.1016/j.molcel.2004.06.019 · Cited by 333 · see citing works
SREBP pathway responds to sterols and functions as an oxygen sensor in fission yeast.
Hughes, A. L., Todd, B. L., & Espenshade, P. J. (2005) · Cell · doi:10.1016/j.cell.2005.01.012 · Cited by 320 · see citing works
Functional dissection and sequence of yeast HAP1 activator
Pfeifer, K. E., Kim, K.-S., Kogan, S. C., & Guarente, L. (1989) · Cell · doi:10.1016/0092-8674(89)90903-3 · Cited by 308 · see citing works
Evolution of a Fungal Regulatory Gene Family: The Zn(II)2Cys6 Binuclear Cluster DNA Binding Motif
Todd, R. B., & Andrianopoulos, A. (1997) · Fungal Genetics and Biology · doi:10.1006/fgbi.1997.0993 · Cited by 295 · see citing works
The B subunit of the DNA polymerase alpha-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication.
Foiani, M., Marini, F., Gamba, D., Lucchini, G., & Plevani, P. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.923 · Cited by 292 · see citing works
Upc2p and Ecm22p, Dual Regulators of Sterol Biosynthesis in Saccharomyces cerevisiae
Vik, Å., & Rine, J. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.19.6395-6405.2001 · Cited by 277 · see citing works
Self-citation
Mutants of GAL4 protein altered in an activation function
Gill, G., & Ptashne, M. (1987) · Cell · doi:10.1016/0092-8674(87)90016-x · Cited by 269 · see citing works
Mutant Mammalian Cells as Tools to Delineate the Sterol Regulatory Element-Binding Protein Pathway for Feedback Regulation of Lipid Synthesis
Goldstein, J. L., Rawson, R. B., & Brown, M. S. (2002) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.2001.2615 · Cited by 250 · see citing works
Integration of PCR Fragments at Any Specific Site within Cloning Vectors without the Use of Restriction Enzymes and DNA Ligase
Geiser, M., Cèbe, R., Drewello, D., & Schmitz, R. (2001) · BioTechniques · doi:10.2144/01311st05 · Cited by 247 · see citing works
Candida albicans Zinc Cluster Protein Upc2p Confers Resistance to Antifungal Drugs and Is an Activator of Ergosterol Biosynthetic Genes
MacPherson, S., Akache, B., Weber, S., De Deken, X., Raymond, M., & Turcotte, B. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.5.1745-1752.2005 · Cited by 238 · see citing works
Genomic Analyses of Anaerobically Induced Genes in Saccharomyces cerevisiae : Functional Roles of Rox1 and Other Factors in Mediating the Anoxic Response
Kwast, K. E., Lai, L., Menda, N. S., James, D. T., Aref, S., & Burke, P. V. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.1.250-265.2002 · Cited by 232 · see citing works
Role of Candida albicans Transcription Factor Upc2p in Drug Resistance and Sterol Metabolism
Silver, P. M., Oliver, B. G., & White, T. C. (2004) · Eukaryotic Cell · doi:10.1128/ec.3.6.1391-1397.2004 · Cited by 216 · see citing works
Multiple Functions of Sterols in Yeast Endocytosis
Heese-Peck, A., Pichler, H., Zanolari, B., Watanabe, R., Daum, G., & Riezman, H. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-04-0186 · Cited by 190 · see citing works
Reciprocal Regulation of Anaerobic and Aerobic Cell Wall Mannoprotein Gene Expression in Saccharomyces cerevisiae
Abramova, N., Sertil, O., Mehta, S., & Lowry, C. V. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.9.2881-2887.2001 · Cited by 157 · see citing works
Regulatory Mechanisms Controlling Expression of the DAN/TIR Mannoprotein Genes During Anaerobic Remodeling of the Cell Wall in Saccharomyces cerevisiae
Abramova, N. E., Cohen, B. D., Sertil, O., Kapoor, R., Davies, K. J. A., & Lowry, C. V. (2001) · Genetics · doi:10.1093/genetics/157.3.1169 · Cited by 128 · see citing works
A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in Saccharomyces cerevisiae
Crowley, J. H., Leak, F. W., Shianna, K. V., Tove, S., & Parks, L. W. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.16.4177-4183.1998 · Cited by 123 · see citing works
The B Subunit of the DNA Polymerase α-Primase Complex in Saccharomyces cerevisiae Executes an Essential Function at the Initial Stage of DNA Replication
Foiani, M., Marini, F., Gamba, D., Lucchini, G., & Plevani, P. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.923-933.1994 · Cited by 114 · see citing works
Transcriptional Regulation by Ergosterol in the Yeast Saccharomyces cerevisiae
Smith, S. J., Crowley, J. H., & Parks, L. W. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.10.5427 · Cited by 91 · see citing works
Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae
Kennedy, M. A., Barbuch, R., & Bard, M. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00035-4 · Cited by 85 · see citing works
Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post‐squalene portion of the yeast ergosterol pathway
Arthington, B. A., Crowell, D. N., Yang, H., Sturley, S. L., & Bard, M. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00807-1 · Cited by 80 · see citing works
Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism
Lewis, T. L., Keesler, G. A., Fenner, G. P., & Parks, L. W. (1988) · Yeast · doi:10.1002/yea.320040203 · Cited by 80 · see citing works
Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.
Dimster-Denk, D., Thorsness, M. K., & Rine, J. (1994) · Molecular Biology of the Cell · doi:10.1091/mbc.5.6.655 · Cited by 78 · see citing works
Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae
Hongay, C., Jia, N., Bard, M., & Winston, F. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf415 · Cited by 72 · see citing works
Transcriptional Regulation of a Sterol-Biosynthetic Enzyme by Sterol Levels in Saccharomyces cerevisiae
Dimster-Denk, D., & Rine, J. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.8.3981 · Cited by 63 · see citing works
Identification of a UPC2 Homolog in Saccharomyces cerevisiae and Its Involvement in Aerobic Sterol Uptake
Shianna, K. V., Dotson, W. D., Tove, S. R., & Parks, L. W. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.3.830-834.2001 · Cited by 63 · see citing works
Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation
Andrau, J., van Oevelen, C. J. C., van Teeffelen, H. A. A. M., Weil, P. A., Holstege, F. C. P., & Timmers, H. T. M. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf485 · Cited by 60 · see citing works
Sterol metabolism and ERG2 gene regulation in the yeast Saccharomyces cerevisiae
Soustre, I., Dupuy, P.-H., Silve, S., Karst, F., & Loison, G. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01300-4 · Cited by 34 · see citing works
Transcriptional regulation of YML083c under aerobic and anaerobic conditions
Linde, J. J. M. T., & Steensma, H. Y. (2003) · Yeast · doi:10.1002/yea.975 · Cited by 18 · see citing works

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Westfall, P. J., Pitera, D. J., Lenihan, J. R., Eng, D., Woolard, F. X., Regentin, R., Horning, T., Tsuruta, H., Melis, D. J., Owens, A. E., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1110740109 · Cited by 726 · see citing works
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Bhattacharya, S., Sae-Tia, S., & Fries, B. C. (2020) · Antibiotics · doi:10.3390/antibiotics9060312 · Cited by 474 · see citing works
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Engels, B., Dahm, P., & Jennewein, S. (2008) · Metabolic Engineering · doi:10.1016/j.ymben.2008.03.001 · Cited by 380 · see citing works
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Moses, T., Pollier, J., Thevelein, J. M., & Goossens, A. (2013) · New Phytologist · doi:10.1111/nph.12325 · Cited by 245 · see citing works
A Gain-of-Function Mutation in the Transcription Factor Upc2p Causes Upregulation of Ergosterol Biosynthesis Genes and Increased Fluconazole Resistance in a Clinical Candida albicans Isolate
Dunkel, N., Liu, T. T., Barker, K. S., Homayouni, R., Morschhäuser, J., & Rogers, P. D. (2008) · Eukaryotic Cell · doi:10.1128/ec.00103-08 · Cited by 236 · see citing works
Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2
Yang, H., Tong, J., Lee, C. W., Ha, S., Eom, S. H., & Im, Y. J. (2015) · Nature Communications · doi:10.1038/ncomms7129 · Cited by 223 · see citing works
Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools
Siddiqui, M. S., Thodey, K., Trenchard, I. J., & Smolke, C. D. (2011) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2011.00774.x · Cited by 226 · see citing works
Telomeric heterochromatin boundaries require NuA4-dependent acetylation of histone variant H2A.Z in SaccharomycescerevisiaePreprint
Babiarz, J., Halley, J. E., & Rine, J. (2006) · Genes & Development · doi:10.1101/gad.1386306 · Cited by 199 · see citing works
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Nohturfft, A., & Zhang, S. C. (2009) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.24.110707.175344 · Cited by 175 · see citing works
Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae
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Redirection of flux through the FPP branch‐point in Saccharomyces cerevisiae by down‐regulating squalene synthase
Paradise, E. M., Kirby, J., Chan, R., & Keasling, J. D. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21766 · Cited by 148 · see citing works
Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors
Montañés, F. M., Pascual‐Ahuir, A., & Proft, M. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07502.x · Cited by 137 · see citing works
Heme Levels Switch the Function of Hap1 of Saccharomyces cerevisiae between Transcriptional Activator and Transcriptional Repressor
Hickman, M. J., & Winston, F. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00887-07 · Cited by 137 · see citing works
A phosphorylated transcription factor regulates sterol biosynthesis in Fusarium graminearum
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Synthesis and turnover of non-polar lipids in yeast
Rajakumari, S., Grillitsch, K., & Daum, G. (2008) · Progress in Lipid Research · doi:10.1016/j.plipres.2008.01.001 · Cited by 136 · see citing works
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A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae
Davies, B. S. J., & Rine, J. (2006) · Genetics · doi:10.1534/genetics.106.059964 · Cited by 123 · see citing works
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Eliaš, D., Tóth Hervay, N., & Gbelská, Y. (2024) · Stresses · doi:10.3390/stresses4040041 · Cited by 63 · see citing works
Synthetic biology strategies toward heterologous phytochemical production
Kotopka, B. J., Li, Y., & Smolke, C. D. (2018) · Natural Product Reports · doi:10.1039/c8np00028j · Cited by 61 · see citing works
Yeast-based genome mining, production and mechanistic studies of the biosynthesis of fungal polyketide and peptide natural products
Tsunematsu, Y., Ishiuchi, K., Hotta, K., & Watanabe, K. (2013) · Natural Product Reports · doi:10.1039/c3np70037b · Cited by 59 · see citing works
Regulation of hypoxia adaptation: an overlooked virulence attribute of pathogenic fungi?
Grahl, N., & Cramer, R. A. (2009) · Medical Mycology · doi:10.3109/13693780902947342 · Cited by 59 · see citing works
Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction
Guo, X., Xiao, W., Wang, Y., Yao, M., Zeng, B., Liu, H. S., Zhao, G.-R., & Yuan, Y. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1194-9 · Cited by 56 · see citing works
UPC2A Is Required for High-Level Azole Antifungal Resistance in Candida glabrata
Whaley, S. G., Caudle, K. E., Vermitsky, J.-P., Chadwick, S. G., Toner, G., Barker, K. S., Gygax, S. E., & Rogers, P. D. (2014) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02217-13 · Cited by 52 · see citing works
Progress and perspectives for microbial production of farnesene
Liu, Y., Wang, Z., Cui, Z., Qi, Q., & Hou, J. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2022.126682 · Cited by 51 · see citing works
Redirection of the Glycolytic Flux Enhances Isoprenoid Production in Saccharomyces cerevisiae
Kwak, S., Yun, E. J., Lane, S., Oh, E. J., Kim, K. H., & Jin, Y. (2019) · Biotechnology Journal · doi:10.1002/biot.201900173 · Cited by 50 · see citing works
Ergosterol reduction impairs mitochondrial DNA maintenance in S. cerevisiae
Cirigliano, A., Macone, A., Bianchi, M. M., Oliaro‐Bosso, S., Balliano, G., Negri, R., & Rinaldi, T. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2018.12.002 · Cited by 47 · see citing works
Induction of DAN/TIR yeast cell wall mannoprotein genes in response to high hydrostatic pressure and low temperature
Abe, F. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.09.039 · Cited by 48 · see citing works
Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae
Marie, C., Leyde, S., & White, T. C. (2008) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2008.07.004 · Cited by 47 · see citing works
Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast
Gu, Y., Xue, J., Ye, L., & Yu, H. (2021) · Bioresources and Bioprocessing · doi:10.1186/s40643-021-00460-9 · Cited by 46 · see citing works
De Novo Synthesis of High-Value Plant Sesquiterpenoids in Yeast
Nguyen, T., MacNevin, G., & Ro, D. (2012) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-404634-4.00013-9 · Cited by 43 · see citing works
Combined Biosynthetic Pathway Engineering and Storage Pool Expansion for High-Level Production of Ergosterol in Industrial Saccharomyces cerevisiae
Sun, Z., Lian, J., Zhu, L., Jiang, Y., Li, G., Xue, H., Wu, M., Yang, L.-R., & Lin, J. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.681666 · Cited by 43 · see citing works
H3K4 methyltransferase Set1 is involved in maintenance of ergosterol homeostasis and resistance to Brefeldin A
South, P. F., Harmeyer, K. M., Serratore, N. D., & Briggs, S. D. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215768110 · Cited by 42 · see citing works
De novo biosynthesis of mogroside V by multiplexed engineered yeasts
Qu, G., Song, Y., Xu, X., Liu, Y., Li, J., Du, G., Liu, L., Li, Y., & Lv, X. (2025) · Metabolic Engineering · doi:10.1016/j.ymben.2025.01.002 · Cited by 43 · see citing works
From Lipid Homeostasis to Differentiation: Old and New Functions of the Zinc Cluster Proteins Ecm22, Upc2, Sut1 and Sut2
Joshua, I., & Höfken, T. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18040772 · Cited by 40 · see citing works
Co-Evolution of Transcriptional Silencing Proteins and the DNA Elements Specifying Their Assembly
Zill, O. A., Scannell, D. R., Teytelman, L., & Rine, J. (2010) · PLoS Biology · doi:10.1371/journal.pbio.1000550 · Cited by 40 · see citing works
The isoprenoid pathway and transcriptional response to its inhibitors in the yeastSaccharomyces cerevisiae
Kuranda, K., François, J., & Palamarczyk, G. (2009) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2009.00560.x · Cited by 40 · see citing works
The Candida glabrata Upc2A transcription factor is a global regulator of antifungal drug resistance pathways
Vu, B. G., Stamnes, M. A., Li, Y., Rogers, P. D., & Moye-Rowley, W. S. (2021) · PLOS Genetics · doi:10.1371/journal.pgen.1009582 · Cited by 39 · see citing works
The response regulator BcSkn7 is required for vegetative differentiation and adaptation to oxidative and osmotic stresses in B otrytis cinerea
Yang, Q., Yin, D., Yin, Y., Cao, Y., & Ma, Z. (2014) · Molecular Plant Pathology · doi:10.1111/mpp.12181 · Cited by 39 · see citing works
Candida albicans UPC2 is transcriptionally induced in response to antifungal drugs and anaerobicity through Upc2p-dependent and -independent mechanisms
Hoot, S. J., Oliver, B. G. G., & White, T. C. (2008) · Microbiology · doi:10.1099/mic.0.2008/017475-0 · Cited by 39 · see citing works
Cumulative Mutations Affecting Sterol Biosynthesis in the Yeast Saccharomyces cerevisiae Result in Synthetic Lethality That Is Suppressed by Alterations in Sphingolipid Profiles
Valachovič, M., Bareither, B. M., Bhuiyan, M. S. A., Eckstein, J. A., Barbuch, R. J., Balderes, D. A., Wilcox, L. J., Sturley, S. L., Dickson, R. C., & Bard, M. (2006) · Genetics · doi:10.1534/genetics.105.053025 · Cited by 39 · see citing works
Structural basis for activation of fungal sterol receptor Upc2 and azole resistance
Tan, L., Chen, L., Yang, H., Jin, B., Kim, G., & Im, Y. J. (2022) · Nature Chemical Biology · doi:10.1038/s41589-022-01117-0 · Cited by 38 · see citing works
Sterol and Steroid Biosynthesis and Metabolism in Plants and Microorganisms
Schaller, H. (2010) · Elsevier eBooks · doi:10.1016/b978-008045382-8.00008-3 · Cited by 38 · see citing works
Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion
Wu, G., Xu, Z., & Jönsson, L. J. (2017) · Microbial Cell Factories · doi:10.1186/s12934-017-0811-9 · Cited by 38 · see citing works
Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency
Jordá, T., Barba‐Aliaga, M., Rozés, N., Alepuz, P. M., Martínez‐Pastor, M. T., & Puig, S. (2022) · Environmental Microbiology · doi:10.1111/1462-2920.16157 · Cited by 35 · see citing works
The C andida glabrata sterol scavenging mechanism, mediated by the ATP ‐binding cassette transporter A us1p, is regulated by iron limitation
Nagi, M., Tanabe, K., Ueno, K., Nakayama, H., Aoyama, T., Chibana, H., Yamagoe, S., Umeyama, T., Oura, T., Ohno, H., et al. (2013) · Molecular Microbiology · doi:10.1111/mmi.12189 · Cited by 34 · see citing works
A Novel Sterol-Signaling Pathway Governs Azole Antifungal Drug Resistance and Hypoxic Gene Repression in Saccharomyces cerevisiae
Serratore, N. D., Baker, K. M., Macadlo, L. A., Gress, A. R., Powers, B. L., Atallah, N., Westerhouse, K. M., Hall, M. C., Weake, V. M., & Briggs, S. D. (2018) · Genetics · doi:10.1534/genetics.117.300554 · Cited by 33 · see citing works
The role of sterols in morphogenetic processes and dimorphism in fungi
Mysyakina, I. S., & Funtikova, N. S. (2007) · Microbiology · doi:10.1134/s0026261707010018 · Cited by 33 · see citing works
Novel Antifungal Drug Discovery Based on Targeting Pathways Regulating the Fungus-Conserved Upc2 Transcription Factor
Gallo-Ebert, C., Donigan, M., Stroke, I. L., Swanson, R. N., Manners, M. T., Francisco, J., Toner, G., Gallagher, D., Huang, C.-Y., Gygax, S. E., et al. (2014) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01677-13 · Cited by 32 · see citing works
Enzyme and Metabolic Engineering Strategies for Biosynthesis of α-Farnesene in Saccharomyces cerevisiae
Wang, S., Zhan, C., Nie, S., Tian, D., Lu, J., Wen, M., Qiao, J., Zhu, H., & Caiyin, Q. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c03677 · Cited by 32 · see citing works
Reengineering of 7-dehydrocholesterol biosynthesis in Saccharomyces cerevisiae using combined pathway and organelle strategies
Wei, W., Gao, S., Yi, Q., Liu, A., Yu, S., & Zhou, J. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.978074 · Cited by 31 · see citing works
Sulphiredoxin plays peroxiredoxin‐dependent and ‐independent roles via the HOG signalling pathway in C ryptococcus neoformans and contributes to fungal virulence
Upadhya, R., Kim, H., Jung, K., Park, G., Lam, W. C., Lodge, J. K., & Bahn, Y. (2013) · Molecular Microbiology · doi:10.1111/mmi.12388 · Cited by 31 · see citing works
In vitro and in vivo antifungal activities and mechanism of heteropolytungstates against Candida species
Li, H., Gong, H., Qi, Y., Li, J., Ji, X., Sun, J., Tian, R., Bao, H., Song, X., Chen, Q., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-17239-8 · Cited by 30 · see citing works
DNA microarray analysis of fluconazole resistance in a laboratory Candida albicans strain
Yan, L., Zhang, J., Li, M., Cao, Y., Xu, Z. Q., Cao, Y., Gao, P., Wang, Y., & Jiang, Y. (2008) · Acta Biochimica et Biophysica Sinica · doi:10.1111/j.1745-7270.2008.00483.x · Cited by 31 · see citing works
Efficient Plant Triterpenoids Synthesis in Saccharomyces cerevisiae : from Mechanisms to Engineering Strategies
Wang, S., Meng, D., Feng, M., Li, C., & Wang, Y. (2024) · ACS Synthetic Biology · doi:10.1021/acssynbio.4c00061 · Cited by 29 · see citing works
Interspecies variation reveals a conserved repressor of α-specific genes in Saccharomyces yeastsPreprint
Zill, O. A., & Rine, J. (2008) · Genes & Development · doi:10.1101/gad.1640008 · Cited by 29 · see citing works
Overexpression of Ecm22 improves ergosterol biosynthesis inSaccharomyces cerevisiae
Wang, S.-Q., Wang, T., Liu, J., Deng, L., & Wang, F. (2018) · Letters in Applied Microbiology · doi:10.1111/lam.13061 · Cited by 28 · see citing works
Regulation of lipid metabolism: a tale of two yeasts
Raychaudhuri, S., Young, B. P., Espenshade, P. J., & Loewen, C. J. R. (2012) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2012.05.006 · Cited by 28 · see citing works
Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs
Martchenko, M., Levitin, A., & Whiteway, M. S. (2006) · Eukaryotic Cell · doi:10.1128/ec.00183-06 · Cited by 26 · see citing works
Azole-Resistant Alleles of ERG11 in Candida glabrata Trigger Activation of the Pdr1 and Upc2A Transcription Factors
Vu, B. G., & Moye-Rowley, S. (2022) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02098-21 · Cited by 28 · see citing works
The UPC2 Promoter in Candida albicans Contains Two cis -Acting Elements That Bind Directly to Upc2p, Resulting in Transcriptional Autoregulation
Hoot, S. J., Brown, R. P., Oliver, B. G., & White, T. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00130-10 · Cited by 25 · see citing works
Regulatory factors controlling transcription of Saccharomyces cerevisiae IXR1 by oxygen levels: a model of transcriptional adaptation from aerobiosis to hypoxia implicating ROX1 and IXR1 cross-regulation
Castro-Prego, R., Maceiras, M. L., Soengas, P., Carneiro, I., González-Siso, M.-I., & Cerdán, M. E. (2009) · Biochemical Journal · doi:10.1042/bj20091500 · Cited by 25 · see citing works
The ABC transporter Pdr18 is required for yeast thermotolerance due to its role in ergosterol transport and plasma membrane properties
Godinho, C. P., Costa, R., & Sá‐Correia, I. (2020) · Environmental Microbiology · doi:10.1111/1462-2920.15253 · Cited by 23 · see citing works
The zinc cluster proteins U pc2 and E cm22 promote filamentation in S accharomyces cerevisiae by sterol biosynthesis‐dependent and ‐independent pathways
Woods, K., & Höfken, T. (2015) · Molecular Microbiology · doi:10.1111/mmi.13244 · Cited by 23 · see citing works
Expanded Roles of the Origin Recognition Complex in the Architecture and Function of Silenced Chromatin in Saccharomyces cerevisiae
Özaydın, B., & Rine, J. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.00614-09 · Cited by 23 · see citing works
The Ins and Outs of Azole Antifungal Drug Resistance: Molecular Mechanisms of Transport
Zavřel, M., Esquivel, B. D., & White, T. C. (2017) · Springer eBooks · doi:10.1007/978-1-4939-0694-9_29 · Cited by 20 · see citing works
Function and Regulation of Cph2 in Candida albicans
Lane, S., Lena, P. D., Tormanen, K., Baldi, P., & Liu, H. (2015) · Eukaryotic Cell · doi:10.1128/ec.00102-15 · Cited by 19 · see citing works
Exploration of biosynthetic access to the shared precursor of the fusicoccane diterpenoid family
Arens, J., Engels, B., Klopries, S., Jennewein, S., Ottmann, C., & Schulz, F. (2013) · Chemical Communications · doi:10.1039/c2cc37154e · Cited by 20 · see citing works
Kluyveromyces lactis: A Suitable Yeast Model to Study Cellular Defense Mechanisms against Hypoxia-Induced Oxidative Stress
González-Siso, M.-I., & Cerdán, M. E. (2012) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2012/634674 · Cited by 19 · see citing works
Combinatorial metabolic engineering of Saccharomyces cerevisiae for improved production of 7-dehydrocholesterol
Gu, Y., Chen, S., Xue, J., Bian, Q., Ye, L., & Yu, H. (2023) · Engineering Microbiology · doi:10.1016/j.engmic.2023.100100 · Cited by 18 · see citing works
The cytochrome b5 dependent C-5(6) sterol desaturase DES5A from the endoplasmic reticulum of Tetrahymena thermophila complements ergosterol biosynthesis mutants in Saccharomyces cerevisiae
Poklépovich, T., Rinaldi, M. A., Tomazic, M. L., Favale, N. O., Turkewitz, A. P., Nudel, C. B., & Nusblat, A. D. (2012) · Steroids · doi:10.1016/j.steroids.2012.08.015 · Cited by 18 · see citing works
Modular and Molecular Optimization of a LOV (Light–Oxygen–Voltage)-Based Optogenetic Switch in Yeast
Romero, A., Rojas, V., Delgado, V., Salinas, F., & Larrondo, L. F. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22168538 · Cited by 17 · see citing works
Common aspects in the engineering of yeasts for fatty acid- and isoprene-based products
Arhar, S., & Natter, K. (2019) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2019.08.009 · Cited by 17 · see citing works
Specific Glycosylation System Enables the High-Level and Sustainable Production of Glycyrrhetinic Acid 3-O-monoglucuronide and Glycyrrhizin in Engineered Saccharomyces cerevisiae
Yang, B., Sun, W., Liu, X., Ding, M., Feng, X., & Li, C. (2024) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.3c06260 · Cited by 16 · see citing works
Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi
Ebert, C., McCourt, P. C., Donigan, M., Villasmil, M. L., Chen, W., Pandya, D., Franco, J., Romano, D. R., Chadwick, S. G., Gygax, S. E., et al. (2011) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2011.11.006 · Cited by 15 · see citing works
Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices
Oger, E., Ghignone, S., Campagnac, E., Fontaine, J., Grandmougin‐Ferjani, A., & Lanfranco, L. (2009) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2009.03.002 · Cited by 14 · see citing works
Metabolic Engineering of Candida glycerinogenes for Sustainable Production of Geraniol
Zhao, C., Wang, X., Lu, X., Zong, H., & Zhuge, B. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00195 · Cited by 13 · see citing works
Mechanisms of sensing and adaptive responses to low oxygen conditions in mammals and yeasts
Trendeleva, T. A., Aliverdieva, D. A., & Zvyagilskaya, R. A. (2014) · Biochemistry (Moscow) · doi:10.1134/s0006297914080033 · Cited by 13 · see citing works
A gain-of-function mutation in zinc cluster transcription factor Rob1 drives Candida albicans adaptive growth in the cystic fibrosis lung environment
Gnaien, M., Maufrais, C., Rebai, Y., Kallel, A., Ma, L., Hamouda, S., Khalsi, F., Meftah, K., Smaoui, H., Khémiri, M., et al. (2024) · PLoS Pathogens · doi:10.1371/journal.ppat.1012154 · Cited by 12 · see citing works
Vacuolar H+-Pyrophosphatase AVP1 is Involved in Amine Fungicide Tolerance in Arabidopsis thaliana and Provides Tridemorph Resistance in Yeast
Hernández, A., Herrera-Palau, R., Madroñal, J. M., Albi, T., López‐Lluch, G., Pérez‐Castiñeira, J. R., Navas, P., Valverde, F., & Serrano, A. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00085 · Cited by 12 · see citing works
The Yeast Anaerobic Response Element AR1b Regulates Aerobic Antifungal Drug-dependent Sterol Gene Expression
Ebert, C., Donigan, M., Liu, H., Pascual, F. L., Manners, M. T., Pandya, D., Swanson, R., Gallagher, D., Chen, W., Carman, G. M., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.526087 · Cited by 11 · see citing works
Secretory IgA reduced the ergosterol contents of Candida albicans to repress its hyphal growth and virulence
Wang, J., Shen, J., Chen, D., Liao, B., Chen, X., Zong, Y., Wei, Y., Shi, Y., Liu, Y., Gou, L., et al. (2024) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-024-13063-z · Cited by 10 · see citing works
Crucial Involvement of Heme Biosynthesis in Vegetative Growth, Development, Stress Response, and Fungicide Sensitivity of Fusarium graminearum
Wang, J., Cao, Y., Shi, D., Zhang, Z., Li, X., & Chen, C. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25105268 · Cited by 10 · see citing works

Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae
Marie, C., Leyde, S., & White, T. C. (2008) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2008.07.004 · Cited by 47 · see citing works
Upc2p and Ecm22p, Dual Regulators of Sterol Biosynthesis in Saccharomyces cerevisiae
Vik, Å., & Rine, J. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.19.6395-6405.2001 · Cited by 277 · see citing works
Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2
Yang, H., Tong, J., Lee, C. W., Ha, S., Eom, S. H., & Im, Y. J. (2015) · Nature Communications · doi:10.1038/ncomms7129 · Cited by 223 · see citing works
Sterol homeostasis in yeast
Rogers, P. D. (2022) · Nature Chemical Biology · doi:10.1038/s41589-022-01143-y · Cited by 5 · see citing works
Transcription factors CgUPC2A and CgUPC2B regulate ergosterol biosynthetic genes in Candida glabrata: Gene expression in sterol-depleted Candida
Nagi, M., Nakayama, H., Tanabe, K., Bard, M., Aoyama, T., Okano, M., Higashi, S., Ueno, K., Chibana, H., Niimi, M., et al. (2011) · Genes to Cells · doi:10.1111/j.1365-2443.2010.01470.x · Cited by 68 · see citing works

Authors: Davies, B. S. J., Wang, H. S., & Rine, J.
Affiliations: University of California, Berkeley (US)
Corresponding author: Rine, Jasper
ORCID: Davies, Brandon S. J.
Rine, Jasper
Venue: Molecular and Cellular Biology
Publisher: Informa UK Limited
Published: 2005-08
Volume: 25
Issue: 16
Pages: 7375-7385
ISSN: 0270-7306 (ISSN-L)
1098-5549
DOI: 10.1128/mcb.25.16.7375-7385.2005
PMC: PMC1190251
OpenAlex: W2110895138
Cited by: 138
References: 42
Citations by year: 2026: 4 · 2025: 4 · 2024: 9 · 2023: 5 · 2022: 7 · 2021: 13 · 2020: 5 · 2019: 4 · 2018: 5 · 2017: 9 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 12 · 2012: 7
FWCI: 2.99
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Molecular and Cellular Biology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
eScholarship (California Digital Library) (submittedVersion)
Funders: National Institute of General Medical Sciences (R01GM035827, T32GM007232)
National Institute of Environmental Health Sciences (T32ES007075)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords: sterol biosynthesis, ergosterol, transactivation domain, transcriptional regulation, ERG gene, sterol depletion, promoter binding, Saccharomyces cerevisiae, transcription factors
MeSH terms: beta-Galactosidase, DNA, Ergosterol, Lac Operon, Models, Biological, Models, Chemical, Models, Genetic, Plasmids, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Sterols, Transcription Factors, Transcription, Genetic, Transcriptional Activation, Trans-Activators, Protein Structure, Tertiary, Genes, Reporter, Saccharomyces cerevisiae Proteins, Chromatin Immunoprecipitation

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Dual Activators of the Sterol Biosynthetic Pathway of Saccharomyces cerevisiae : Similar Activation/Regulatory Domains but Different Response Mechanisms

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