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Cited by 1,764Ubiquitin and proteasome pathwaysHeat shock proteins researchProtein Degradation and Inhibitors

Recognition and Processing of Ubiquitin-Protein Conjugates by the Proteasome

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Finley, Daniel·2009·Annual Review of Biochemistry
DOI 10.1146/annurev.biochem.78.081507.101607PMC PMC3431160
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Abstract

The proteasome is an intricate molecular machine, which serves to degrade proteins following their conjugation to ubiquitin. Substrates dock onto the proteasome at its 19-subunit regulatory particle via a diverse set of ubiquitin receptors and are then translocated into an internal chamber within the 28-subunit proteolytic core particle (CP), where they are hydrolyzed. Substrate is threaded into the CP through a narrow gated channel, and thus translocation requires unfolding of the substrate. Six distinct ATPases in the regulatory particle appear to form a ring complex and to drive unfolding as well as translocation. ATP-dependent, degradation-coupled deubiquitination of the substrate is required both for efficient substrate degradation and for preventing the degradation of the ubiquitin tag. However, the proteasome also contains deubiquitinating enzymes (DUBs) that can remove ubiquitin before substrate degradation initiates, thus allowing some substrates to dissociate from the proteasome and escape degradation. Here we examine the key elements of this molecular machine and how they cooperate in the processing of proteolytic substrates.

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Authors
Finley, D.
Affiliations
Harvard University (US)
Corresponding author
Finley, Daniel
ORCID
Finley, Daniel
Venue
Annual Review of Biochemistry
Publisher
Annual Reviews
Published
2009-06-01
Volume
78
Issue
1
Pages
477-513
ISSN
  • 0066-4154 (ISSN-L)
  • 1545-4509
DOI
10.1146/annurev.biochem.78.081507.101607
PMC
PMC3431160
OpenAlex
W2097164847
Cited by
1,764
References
4
Citations by year
2026: 55 · 2025: 74 · 2024: 86 · 2023: 97 · 2022: 96 · 2021: 96 · 2020: 110 · 2019: 96 · 2018: 100 · 2017: 94 · 2016: 110 · 2015: 111 · 2014: 134 · 2013: 129 · 2012: 145
FWCI
9.57
Type
journal article
Language
en
Open access
Locations
  • Annual Review of Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of General Medical Sciences (R01GM043601, GM43601, R01GM065592, GM065592, R37GM043601)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK 082906, R21DK082906)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Degradation and Inhibitors
Keywords
proteasome, ubiquitin conjugates, protein degradation, ubiquitin receptors, ATPase, substrate unfolding, substrate translocation, deubiquitination, deubiquitinase
MeSH terms
Animals, Humans, Proteins, Arabidopsis, Ubiquitin, Proteasome Endopeptidase Complex

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