Regulation of placental amino acid transporter activity by mammalian target of rapamycin
Abstract
The activity of placental amino acid transporters is decreased in intrauterine growth restriction (IUGR), but the underlying regulatory mechanisms have not been established. Inhibition of the mammalian target of rapamycin (mTOR) signaling pathway has been shown to decrease the activity of the system L amino acid transporter in human placental villous fragments, and placental mTOR activity is decreased in IUGR. In the present study, we used cultured primary trophoblast cells to study mTOR regulation of placental amino acid transporters in more detail and to test the hypothesis that mTOR alters amino acid transport activity by changes in transporter expression. Inhibition of mTOR by rapamycin significantly reduced the activity of system A (−17%), system L (−28%), and taurine (−40%) amino acid transporters. mRNA expression of isoforms of the three amino acid transporter systems in response to mTOR inhibition was measured using quantitative real-time PCR. mRNA expression of l-type amino acid transporter 1 (LAT1; a system L isoform) and taurine transporter was reduced by 13% and 50%, respectively; however, mTOR inhibition did not alter the mRNA expression of system A isoforms (sodium-coupled neutral amino acid transporter-1, -2, and -4), LAT2, or 4F2hc. Rapamycin treatment did not significantly affect the protein expression of any of the transporter isoforms. We conclude that mTOR signaling regulates the activity of key placental amino acid transporters and that this effect is not due to a decrease in total protein expression. These data suggest that mTOR regulates placental amino acid transporters by posttranslational modifications or by affecting transporter translocation to the plasma membrane.
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The mTOR pathway regulates the activity of system A, system L and taurine amino acid transporters in the placenta at the post-translational level, either through modifications or by influencing translocation to the apical membrane.
“In addition, the mTOR pathway regulates the activity of system A, system L and taurine amino acid transporters in the placenta at the post-translational level, either through modifications or by influencing translocation to the apical membrane (469).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [469]
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RETRACTED: Klotho Gene and Protein in Human Placentas According to Birth Weight and Gestational Age
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- Authors
- Roos, S., Kanai, Y., Prasad, P. D., Powell, T. L., & Jansson, T.
- Affiliations
- University of Gothenburg (SE)
- The University of Osaka (JP)
- University of Cincinnati (US)
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2009-01
- Volume
- 296
- Issue
- 1
- Pages
- C142-C150
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2131629100
- Cited by
- 150
- References
- 69
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 6 · 2023: 5 · 2022: 6 · 2021: 12 · 2020: 12 · 2019: 10 · 2018: 8 · 2017: 13 · 2016: 10 · 2015: 11 · 2014: 12 · 2013: 8 · 2012: 16
- FWCI
- 5.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Keywords
- mTOR signaling, fetal growth restriction, system L amino acid transporter, system A amino acid transporter, taurine transporter, LAT1, rapamycin, trophoblast, post-translational regulation, placenta
- MeSH terms
- Taurine Transporters, Cell Survival, Cells, Cultured, Female, Humans, Membrane Glycoproteins, Pregnancy, Protein Kinases, Protein Processing, Post-Translational, RNA, Messenger, Time Factors, Trophoblasts, Signal Transduction, Apoptosis, Sirolimus, Protein Transport, Membrane Transport Proteins, Amino Acid Transport Systems, Amino Acid Transport System A, Amino Acid Transport System L, TOR Serine-Threonine Kinases
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