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Cited by 142Estrogen and related hormone effectsMetabolism, Diabetes, and CancerAdipose Tissue and Metabolism

Effect of the ovarian hormones on GLUT4 expression and contraction-stimulated glucose uptake

Campbell, S. E., Febbraio, M. A.·2002·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00184.2001
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Abstract

This study examined the roles of the female sex steroids, 17β-estradiol (E 2) and progesterone (Prog), on glucose uptake and GLUT4 protein expression. Female Sprague-Dawley rats were either sham operated (C) or ovariectomized and treated with placebo (O), E 2(E), Prog (P), or both hormones at physiological doses (P + E) or the same dose of Prog with a high dose of E 2(P + HiE) via timed-release pellets inserted at the time of surgery, 15 days before metabolic testing. On the morning of day 15, animals received a 300-μCi injection (ip) of 2-deoxy-[14 C]glucose and then either exercised on a motorized treadmill for 30 min at 0.35 m/s or remained sedentary in their cages for the same period. Basal glucose uptake was not different between the treatment groups in either the red or white quadriceps. However, glucose uptake was decreased ( P < 0.05) in O, P, and P + E rats during exercise in the red quadriceps compared with C rats, whereas E and P + HiE treatment restored glucose uptake. Glycogen content in skeletal muscle followed similar trends, with no differences seen in resting animals. Postexercise red quadriceps glycogen levels were higher ( P < 0.05) in the E and P + HiE rats compared with O and P. Treatment of ovariectomized rats with progesterone (P rats) decreased ( P < 0.05) GLUT4 content in the red quadriceps by 21% compared with C rats. These data demonstrate that estrogen-deficient animals have a decreased ability for contraction-stimulated glucose uptake and increased glycogen use during aerobic exercise. However, changes in contraction-stimulated glucose uptake could not be explained by altered transporter protein content, since the absence of E 2 had no effect on GLUT4 protein.

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Crossref · 10.1152/ajpendo.00184.2001

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  • Progesterone functions catabolically to increase amino acid oxidation and decreases muscle protein synthesis.

    “functions catabolically to increase amino acid oxidation and decreases muscle protein synthesis [78–81]”

    From International society of sports nutrition position stand: nutritional concerns of the female athlete (2023) · cites this paper as [81]

  • Progesterone exerts influence over protein metabolism, specifically during the luteal phase, corresponding with increases in protein oxidation at rest and during exercise at this time in the menstrual cycle.

    “Progesterone also exerts influence over protein metabolism, specifically during the luteal phase, which corresponds with the previously observed increases in protein oxidation at rest and during exercise at this time in the menstrual cycle [81,164].”

    From International society of sports nutrition position stand: nutritional concerns of the female athlete (2023) · cites this paper as [81]

  • In eumenorrheic women, protein catabolism is higher at rest and following aerobic endurance exercise in the luteal phase, when estrogen and progesterone are elevated, compared to the early follicular phase when estrogen and progesterone concentrations are low.

    “In eumenorrheic women, evidence suggests protein catabolism is higher at rest and following aerobic endurance exercise in the luteal phase, when estrogen and progesterone are elevated, compared to the early follicular phase when estrogen and progesterone concentrations are low [81,164,223,224].”

    From International society of sports nutrition position stand: nutritional concerns of the female athlete (2023) · cites this paper as [81]

  • Females require more lysine during the luteal phase than the follicular phase and have a lower ability to uptake and utilize amino acids for protein synthesis.

    “females require more lysine during the luteal phase than the follicular phase [81] with a lower ability to uptake and utilize amino acids for protein synthesis.”

    From International society of sports nutrition position stand: nutritional concerns of the female athlete (2023) · cites this paper as [81]

Cites

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Research works printed but not linked: 2

  1. Cagnacci A, 1992, J Clin Endocrinol Metab, 74, 1396
  2. Elkind Hirsch KE, 1993, J Clin Endocrinol Metab, 76, 472

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Related

Authors
Campbell, S. E., & Febbraio, M. A.
Affiliations
The University of Melbourne (AU)
ORCID
Febbraio, M. A.
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2002-05-01
Volume
282
Issue
5
Pages
E1139-E1146
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00184.2001
OpenAlex
W2112665865
Semantic Scholar
8786fa5da249859947f5bc875ed89357abc9e2b1
Cited by
142
References
42
Citations by year
2026: 4 · 2025: 6 · 2024: 12 · 2023: 10 · 2022: 11 · 2021: 6 · 2020: 8 · 2019: 6 · 2018: 7 · 2017: 8 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 7 · 2012: 4
FWCI
2.28
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
estradiol, progesterone, ovarian hormones, GLUT4, glucose uptake, skeletal muscle, physical activity, ovariectomy, glycogen, female sex hormones, skeletal muscle glucose transport
MeSH terms
Animals, Deoxyglucose, Estradiol, Physical Exertion, Female, Glucose, Glycogen, Insulin, Monosaccharide Transport Proteins, Muscle Contraction, Muscle Proteins, Ovariectomy, Progesterone, Rats, Sprague-Dawley, Muscle, Skeletal, Lactic Acid, Glucose Transporter Type 4, Rats

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