Progesterone reduces the migration of mast cells toward the chemokine stromal cell-derived factor-1/CXCL12 with an accompanying decrease in CXCR4 receptors.
Abstract
Mast cell recruitment is implicated in many physiological functions and several diseases. It depends on microenvironmental factors, including hormones. We have investigated the effect of progesterone on the migration of HMC-1(560) mast cells toward CXCL12, a chemokine that controls the migration of mast cells into tissues. HMC-1(560) mast cells were incubated with 1 nM to 1 microM progesterone for 24 h. Controls were run without progesterone. Cell migration toward CXCL12 was monitored with an in vitro assay, and statistical analysis of repeated experiments revealed that progesterone significantly reduced cell migration without increasing the number of apoptotic cells (P = 0.0084, n = 7). Differences between progesterone-treated and untreated cells were significant at 1 microM (P < 0.01, n = 7). Cells incubated with 1 microM progesterone showed no rearrangment of actin filaments in response to CXCL12. Progesterone also reduced the calcium response to CXCL12 and Akt phosphorylation. Cells incubated with progesterone had one-half the control concentrations of CXCR4 (mRNA, total protein, and membrane-bound protein). Progesterone also inhibited the migration of HMC-1(560) cells transfected with hPR-B-pSG5 plasmid, which contained 2.5 times as much PR-B as the control. These transfected cells responded differently (P < 0.05, n = 5) from untreated cells to 1 nM progesterone. We conclude that progesterone reduces mast cell migration toward CXCL12 and that CXCR4 may be a progesterone target in mast cells.
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HMC-1 cells and BMMCs express both estradiol and progesterone receptors.
“As previously reported, HMC-1 and BMMCs express both, E2 and P4 receptors [27], [28], [24] (Fig. 3E–G).”
From Estradiol and Progesterone Regulate the Migration of Mast Cells from the Periphery to the Uterus and Induce Their Maturation and Degranulation (2010) · cites this paper as [24]
In rat, the active angiogenic process that takes place in the uterine cervix during gestation is regulated by secretion of contents stored in mast cell granules.
“In rat, it has been shown that the active angiogenic process that takes place in the uterine cervix during gestation is regulated by secretion of contents stored in MCs granules [24].”
From Estradiol and Progesterone Regulate the Migration of Mast Cells from the Periphery to the Uterus and Induce Their Maturation and Degranulation (2010) · cites this paper as [24]
Mast cells from peripheral blood migrate into tissues as committed progenitors and develop into mature cells in situ.
“MCs from peripheral blood migrate into tissues as committed progenitors and develop into mature cells in situ [24].”
From Estradiol and Progesterone Regulate the Migration of Mast Cells from the Periphery to the Uterus and Induce Their Maturation and Degranulation (2010) · cites this paper as [24]
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Thevenot, P. (2010) · UTA ResearchCommons (University of Texas Arlington)
- Authors
- Belot, M.-P., Abdennebi-Najar, L., Gaudin, F., Lieberherr, M., Godot, V., Taïeb, J., Emilie, D., & Machelon, V.
- Affiliations
- Inserm (FR)
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2007-05
- Volume
- 292
- Issue
- 5
- Pages
- E1410-E1417
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2167932732
- Cited by
- 29
- References
- 50
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 1 · 2016: 3 · 2015: 3 · 2012: 3
- FWCI
- 1.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Keywords
- progesterone, mast cell migration, CXCL12, CXCR4, progesterone receptor B, chemokine signaling, calcium response, Akt phosphorylation, actin cytoskeleton, mast cell recruitment
- MeSH terms
- Wortmannin, Actins, Androstadienes, Calcium, Cell Movement, Flow Cytometry, Humans, Mast Cells, Phosphorylation, Progesterone, Receptors, Progesterone, RNA, Messenger, Blotting, Western, Receptors, CXCR4, Chemokines, CXC, Reverse Transcriptase Polymerase Chain Reaction, Protein Kinase Inhibitors, Oncogene Protein v-akt, Chemokine CXCL12
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