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Cited by 60Metabolism, Diabetes, and CancerCardiovascular and exercise physiologyMuscle metabolism and nutrition

Postcontraction insulin sensitivity: relationship with contraction protocol, glycogen concentration, and 5′ AMP-activated protein kinase phosphorylation

Kim, Junghoon, Solis, Raquel S., Arias, Edward B., Cartee, Gregory D.·2004·Journal of Applied Physiology
DOI 10.1152/japplphysiol.00909.2003
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Abstract

Exercise enhances insulin-stimulated glucose transport (GT) in skeletal muscle. Evidence suggests that 5′ AMP-activated protein kinase (AMPK) and glycogen may be important for enhanced insulin sensitivity. Our goals were to investigate the effect of various in situ muscle contraction protocols on insulin-stimulated GT and assess the relationship of contraction-induced changes in AMPK and glycogen with postcontraction improvement in insulin-stimulated GT. Rats were anesthetized, both ulnar nerves were exposed, and one nerve was electrically stimulated to contract forelimb muscles. We performed a series of five experiments, sequentially varying only one contraction parameter (train duration, train rate, pulse frequency, number of 5-min bouts, or pulse duration) while holding the others constant. Both epitrochlearis muscles were dissected out and incubated for 3.5 h before measurement of GT. For each contraction parameter studied, we identified an apparent threshold value that did not induce a significant increase in insulin-stimulated GT and an apparent peak value, above which there was a plateau or decline in insulin-stimulated GT. Using other rats, we evaluated muscle AMPK phosphorylation and glycogen concentration immediately postcontraction. AMPK phosphorylation and reduction in glycogen were increased compared with resting controls in each protocol, which had previously been shown to increase insulin-stimulated GT, as well as in several protocols that did not significantly increase insulin-stimulated GT. These data suggest that contraction-induced AMPK phosphorylation and decrease in glycogen may be necessary but are not sufficient for the postcontraction increase in insulin-stimulated GT in rat skeletal muscle.

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Crossref · 10.1152/japplphysiol.00909.2003

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  • Improvements in insulin sensitivity were not consistent with AMPK activation and glycogen depletion.

    “Furthermore, improvements in insulin sensitivity were not consistent with AMPK activation and glycogen depletion [144].”

    From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [144]

  • Different contraction protocols altered the level of AMPK activation, but there were no differences in the level of improvement in insulin sensitivity.

    “Similarly, Kim et al. [144] showed that whilst the different contraction protocols altered the level of AMPK activation, there were no differences in the level of improvement in insulin sensitivity.”

    From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [144]

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Authors
Kim, J., Solis, R. S., Arias, E. B., & Cartee, G. D.
Affiliations
  • Department of Kinesiology and Biodynamics Laboratory, University of Wisconsin, Madison, Wisconsin 53706
  • University of Wisconsin–Madison (US)
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
2004-02
Volume
96
Issue
2
Pages
575-583
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/japplphysiol.00909.2003
OpenAlex
W2104819829
Cited by
60
References
40
Citations by year
2026: 1 · 2023: 1 · 2022: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2016: 6 · 2015: 3 · 2013: 4 · 2012: 1
FWCI
2.37
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute on Aging (R01 AG-10026, R01AG010026)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords
insulin-stimulated glucose transport, muscle glycogen, neuromuscular electrical stimulation, epitrochlearis muscle, skeletal muscle
MeSH terms
Animals, Biological Transport, Electric Stimulation, Glucose, Glycogen, Hypoglycemic Agents, Insulin, Male, Multienzyme Complexes, Muscle Contraction, Phosphorylation, Specific Pathogen-Free Organisms, Rats, Wistar, Protein Serine-Threonine Kinases, Muscle, Skeletal, Rats, AMP-Activated Protein Kinases

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