Cited by 44Muscle Physiology and DisordersExtracellular vesicles in diseaseExercise and Physiological Responses

Roles of exosome-like vesicles released from inflammatory C2C12 myotubes: regulation of myocyte differentiation and myokine expression

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Kim, Sujin, Lee, Min-Jae, Choi, Ji-Young, Park, Dong-Ho, Kwak, Hyo-Bum, Moon, Sohee, Koh, Je-Woo, Shin, Hun-Kyu +4 more·2018·Cellular Physiology and Biochemistry

Abstract

BACKGROUND/AIMS: The complicated differentiation processes of cells in skeletal muscle against inflammation that induce muscle atrophy are not fully elucidated. Given that skeletal muscle is a secretory organ, we evaluated the effects of inflammation on myogenic signals and myokine expression, and the roles of inflammatory exosomes released by myotubes in myogenic differentiation. METHODS: Inflammation was induced by treatment of fully differentiated C2C12 myotubes with a cytokine mixture of TNF-α and INF-γ. Exosome-like vesicles (ELVs) were isolated from conditioned media of control or inflamed myotubes and incubated with myoblasts. The expression of molecular switches that contribute to myogenic differentiation, including several kinases, their downstream targets, and myokines, were evaluated using immunoblot analysis in inflamed myotubes and in myoblasts treated with ELVs. RESULTS: Inflammation activated molecular mechanisms contributing to muscle atrophy, including AMPK, p-38 MAPK and JNK, while inhibiting Akt-mediated myogenic signals. In addition, inflammation induced myostatin expression with suppression of a myostatin-counteracting myokine, decorin. Well-characterized ELVs released from inflamed myotubes induced myoblast inflammation and inhibited myogenic mechanisms while stimulating atrophic signals. CONCLUSION: Inflammation of skeletal muscle induces muscle atrophy via multiple mechanisms, including the regulation of myokines and kinases. Inflammatory ELVs are likely to contribute to inflammation-induced muscle atrophy.

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Crossref · 10.1159/000492505

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  • ELV-MT participate in the maintenance and regeneration of SkM mass following injuries.

    “suggesting that in addition to myogenesis, ELV-MT would also participate in the maintenance and regeneration of SkM mass following injuries.”

    From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Kim et al., 2018

  • Similar results are obtained when myotubes are treated with a pro-inflammatory cytokine mixture of TNF-alpha and INF-gamma.

    “Similar results were obtained when myotubes were treated with a pro-inflammatory cytokine mixture of TNF-alpha and INF-gamma”

    From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Kim et al., 2018

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Authors
Kim, S., Lee, M.-J., Choi, J.-Y., Park, D.-H., Kwak, H.-B., Moon, S., Koh, J.-W., Shin, H.-K., Ryu, J.-K., Park, C.-S., Park, J. H., & Kang, J.-H.
Affiliations
  • Inha University (KR)
  • Kangbuk Samsung Hospital (KR)
Venue
Cellular Physiology and Biochemistry
Publisher
S. Karger AG
Published
2018
Volume
48
Issue
5
Pages
1829-1842
ISSN
  • 1015-8987 (ISSN-L)
  • 1421-9778
OpenAlex
W2885125141
Cited by
44
References
52
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2023: 8 · 2022: 9 · 2021: 9 · 2020: 5 · 2019: 5 · 2018: 1
FWCI
1.78
Type
journal article
Language
en
Open access
Locations
  • Cellular Physiology and Biochemistry (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
exosome-like nanoparticles, skeletal muscle inflammation, muscle atrophy, myogenesis, myokines, myostatin, decorin, TNF-α, interferon-gamma, AMPK, p38 MAPK, JNK, Akt signaling, C2C12 myotubes, myoblasts, extracellular vesicles
MeSH terms
Autophagy-Related Proteins, Animals, Cell Differentiation, Cell Line, Gene Expression Regulation, Phosphorylation, Signal Transduction, Cytokines, MyoD Protein, Myogenin, Muscle Fibers, Skeletal, Myoblasts, JNK Mitogen-Activated Protein Kinases, p38 Mitogen-Activated Protein Kinases, Proto-Oncogene Proteins c-akt, Mice, Cell-Derived Microparticles, AMP-Activated Protein Kinases, Myostatin, Decorin

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