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Cited by 120Pregnancy and preeclampsia studiesNitric Oxide and Endothelin EffectsNeurological Complications and Syndromes

Elevated Levels of S -Nitrosoalbumin in Preeclampsia Plasma

Tyurin, Vladimir A., Liu, Shang-Xi, Tyurina, Yulia Y., Sussman, Nancy B., Hubel, Carl A., Roberts, James M., Taylor, Robert N., Kagan, Valerian E.·2001·Circulation Research
DOI 10.1161/hh1101.092179
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Abstract

Abstract —The availability of nitric oxide (NO), which is required for the normal regulation of vascular tone, may be decreased in preeclampsia, thus contributing to the vascular pathogenesis of this pregnancy disorder. Because ascorbate is essential for the decomposition of S -nitrothiols and the release of NO, we speculated that the ascorbate deficiency typical of preeclampsia plasma might result in decreased rates of decomposition of S -nitrosothiols. We tested the hypothesis that total S -nitrosothiol and S -nitrosoalbumin concentrations are increased in preeclampsia plasma, reflecting a decreased release of NO from these major reservoirs of NO. Gestationally matched plasma samples were obtained (before labor or intravenous MgSO 4 ) from 21 women with preeclampsia and 21 women with normal pregnancy, and plasma samples were also obtained from 12 nonpregnant women of similar age and body mass index during the follicular phase of the menstrual cycle. All were nonsmokers. The assay included ultraviolet-induced decomposition of S -nitrosothiols to liberate NO captured by a florigenic reagent, 4,5-diaminofluoresceine, to produce diaminofluoresceine-Triazole. Preeclampsia plasma contained significantly higher concentrations of total S -nitrosothiols (11.1±2.9 nmol/mL) than normal pregnancy samples (9.4±1.5 nmol/mL). Even greater differences were found between preeclampsia plasma and plasma samples from normal pregnancies and nonpregnant women (294±110, 186±25, and 151±25 pmol/mg protein, respectively) when S -nitrosothiol content was expressed per milligram protein. The albumin fraction contained 49.4% of total plasma S -nitrosothiols in the control samples and 53.7% and 56.8% of plasma S -nitrosothiols in normal pregnancy and preeclampsia, respectively. The level of S -nitrosoalbumin was significantly higher in preeclampsia than in normal pregnancy or nonpregnancy plasma (6.3±1.4, 5.1±0.7, and 4.2±1.0 nmol/mL, respectively). The increased concentration of S -nitrosoalbumin in preeclampsia almost completely accounted for the increased levels of S -nitrosothiols in total plasma. Due to combined increases in nitrosothiols and decreases in protein, the preeclampsia plasma concentration of S -nitrosoalbumin was greatly increased on a per milligram of protein basis (271% and 186% compared with normal nonpregnancy and normal pregnancy plasma, respectively). We conclude that S -nitrosoalbumin and total S -nitrosothiol concentrations are significantly increased in preeclampsia plasma and may reflect insufficient release of NO groups in this condition.

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  • At high ADMA concentrations, l-arginine supplementation may not overcome eNOS inhibition, indicating the need to determine l-arginine/ADMA ratios to better assess l-arginine availability.

    “At high ADMA concentrations, l-arginine supplementation may not overcome eNOS inhibition, indicating the need to determine l-arginine/ADMA ratios to better assess l-arginine availability (102, 106, 954).”

    From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [954]

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Research works printed but not linked: 4

  1. Uotila JT, Solakivi T, Jaakkola O, Tuimala R, Lehtimaki T. Antibodies against copper-oxidized and malondialdehyde-modified low density lipoproteins in preeclamptic pregnancies. Br J Obstet Gynecol. 1998;105: 1113-1117.
  2. Chambers JC, McGregor A, Jean-Marie J, Obeid OA, Kooner JS. Dem- onstration of rapid onset vascular endothelial dysfunction after hyperho- mocysteinemia: an effect reversible with vitamin C therapy. Circulation. 1999;99:1156 -1160.
  3. Tyurin VA, Tyurina YY, Liu SX, Hubel CA, Kagan VE. Fluorescence assay for quantitation of S-nitrosothiols in biological fluids and cells. Methods Enzymol. In press.
  4. Tyurin VA, Borisenko GG, Fabisiak JP, Hubel CA, McLaughlin MK, Roberts JM, Kagan VE. Free fatty acid (FFA-)-induced mishandling of copper (Cu) by serum albumin in preeclampsia: contribution of oxidative stress. Toxicologist. 1999;48:506. Abstract.

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Authors
Tyurin, V. A., Liu, S.-X., Tyurina, Y. Y., Sussman, N. B., Hubel, C. A., Roberts, J. M., Taylor, R. N., & Kagan, V. E.
Affiliations
  • University of Pittsburgh (US)
  • From the Department of Environmental and Occupational Health (V.A.T., S.-X.L., Y.Y.T., N.B.S., V.E.K.) and the Magee-Womens Research Institute and Department of Obstetrics and Gynecology (C.A.H., J.M.R., V.E.K.), University of Pittsburgh, Pittsburgh, Pa, and the Department of Obstetrics/Gynecology and Reproductive Sciences, University of California, San Francisco (R.N.T.).
ORCID
Venue
Circulation Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2001-06-08
Volume
88
Issue
11
Pages
1210-1215
ISSN
  • 0009-7330 (ISSN-L)
  • 1524-4571
DOI
10.1161/hh1101.092179
OpenAlex
W2156597317
Cited by
120
References
34
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 2 · 2018: 4 · 2017: 1 · 2016: 5 · 2015: 2 · 2014: 4 · 2013: 6 · 2012: 4
FWCI
16.22
Type
journal article
Language
en
Open access
closed
Locations
  • Circulation Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Magee-Womens Research Institute
  • National Institutes of Health (5r01hl064145-04)
  • National Heart, Lung, and Blood Institute (1RO1HL64145-01A1, 1RO1HL56829-01)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (P01HD030367, 5PO1HD30367-06)
Topics
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Psychiatry and Mental health › Neurological Complications and Syndromes
Keywords
preeclampsia, S-nitrosothiols, nitric oxide, ascorbate deficiency, nitric oxide release, vascular dysfunction
MeSH terms
Adult, Analysis of Variance, Ascorbic Acid Deficiency, Blood Proteins, Electrophoresis, Polyacrylamide Gel, Female, Fluorescent Dyes, Fluorometry, Humans, Mercaptoethanol, Nitric Oxide, Nitroso Compounds, Pre-Eclampsia, Pregnancy, Pregnancy Trimester, Second, Pregnancy Trimester, Third, Reference Values, Sensitivity and Specificity, Serum Albumin, Serum Albumin, Bovine, Body Mass Index, Oxidative Stress, S-Nitrosothiols

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