Tissue distribution of beta 3-adrenergic receptor mRNA in man
Open accessAbstract
Expression of mRNA for beta 1-, beta 2-, and beta 3-adrenergic receptors (beta 1-, beta 2-, and beta 3-AR) was investigated in human tissues. beta 1- and beta 2-AR mRNA distribution correlated with that of the cognate receptors established by pharmacological studies. beta 3-AR transcripts were abundant in infant perirenal brown adipose tissue, characterized by the presence of uncoupling protein (UCP) mRNA. In adult whole adipose tissues, beta 3-AR mRNA levels were high in deep deposits such as perirenal and omental, and lower in subcutaneous. In these deposits, UCP mRNA levels paralleled those of beta 3-AR. However, isolated omental and subcutaneous adipose cells, enriched in white adipocytes, expressed beta 3-AR but no UCP transcripts. beta 3-AR mRNA was highly expressed in gallbladder, and to a much lower extent in colon, independently of UCP mRNA. Quadriceps or abdominal muscles, heart, liver, lung, kidney, thyroid, and lymphocytes did not express intrinsic beta 3-AR mRNA. This study demonstrates that substantial amounts of brown adipocytes exist throughout life in adipose deposits, which are generally classified as white. These deposits are the main sites of beta 3-AR expression, which also occurs in gallbladder and colon. beta 3-AR may thus be involved in the control of lipid metabolism, possibly from fat assimilation in the digestive tract, to triglyceride storage and mobilization in adipose tissues.
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These end-effects could be of particular relevance in humans given that VAT adipocytes have higher expression of β3 adrenergic receptors than those in scAT.
“These end-effects could be of particular relevance in humans given that VAT adipocytes have higher expression of β3 adrenergic receptors than those in scAT [252].”
From The Role of Exercise in the Interplay between Myokines, Hepatokines, Osteokines, Adipokines, and Modulation of Inflammation for Energy Substrate Redistribution and Fat Mass Loss: A Review (2020) · cites this paper as [252]
The Results (page not provided) report generally higher β3-adrenergic receptor mRNA in omental than subcutaneous fat deposits. They show that isolated adipocytes from both sites express the receptor, but do not establish that omental adipocytes themselves have higher expression; the whole-tissue comparison may also reflect differences in tissue composition. (LLM, conf=0.83)
Experimental — accuracy not yet measuredThe relatively low levels of β-adrenergic receptor expression in human scAT compared with VAT could account for the lack of exercise-induced browning.
“the relatively low levels of β-adrenergic receptor expression in human scAT compared with those in VAT [252] could account for the lack of exercise-induced browning.”
From The Role of Exercise in the Interplay between Myokines, Hepatokines, Osteokines, Adipokines, and Modulation of Inflammation for Energy Substrate Redistribution and Fat Mass Loss: A Review (2020) · cites this paper as [252]
The Results report that β3-adrenergic receptor mRNA was generally lower in human subcutaneous fat than in omental fat, supporting the depot comparison. The same passage says β1- and β2-receptor mRNA levels were comparable across adult adipose locations, so the finding does not extend to β-adrenergic receptors generally. The citation is attached to the expression comparison; the proposed explanation for exercise-induced browning is the citing authors’ inference. (LLM, conf=0.87)
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Self-citation
- Authors
- Krief, S., Lönnqvist, F., Raimbault, S., Baude, B., Van Spronsen, A., Arner, P., Strosberg, A. D., Ricquier, D., & Emorine, L. J.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Journal of Clinical Investigation
- Published
- 1993-01-01
- Volume
- 91
- Issue
- 1
- Pages
- 344-349
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC330032
- OpenAlex
- W2117821842
- Cited by
- 528
- References
- 32
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 11 · 2023: 8 · 2022: 5 · 2021: 11 · 2020: 11 · 2019: 14 · 2018: 10 · 2017: 9 · 2016: 13 · 2015: 7 · 2014: 20 · 2013: 11 · 2012: 8
- FWCI
- 19.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Sveriges Läkarförbund
- Medical Research Council
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- beta3-adrenergic receptor, receptor mRNA distribution, human tissue, brown adipose tissue, uncoupling proteins, omental adipose tissue, subcutaneous adipose tissue, gallbladder, colon, lipid metabolism, triglyceride storage, white adipocytes
- MeSH terms
- Adipose Tissue, Adult, Aged, Base Sequence, Child, Child, Preschool, Female, Heart, Humans, Infant, Infant, Newborn, Male, Middle Aged, Molecular Sequence Data, Organ Specificity, Receptors, Adrenergic, beta, RNA, Messenger, Blotting, Northern, Polymerase Chain Reaction, Oligonucleotides, Antisense
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