The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds
Abstract
A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method.
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Azhim et al. utilized sonication combined with SDS to decellularize porcine aorta.
“Azhim et al. [25] utilized sonication combined with SDS to decellularize porcine aorta”
From Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (<i>Oreochromis niloticus</i>) Viscera (2024) · cites this paper as [25]
Sonication disrupts cell membranes more effectively, making them more permeable to detergent action.
“suggesting that sonication disrupts cell membranes more effectively, making them more permeable to detergent action [25].”
From Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (<i>Oreochromis niloticus</i>) Viscera (2024) · cites this paper as [25]
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Self-citationMatrix Biology of the Salivary Gland: A Guide for Tissue Engineering
Martinez, M., Wu, D., Farach‐Carson, M. C., & Harrington, D. A. (2017) · Springer eBooks · doi:10.1007/978-3-319-43513-8_8 · Cited by 2 · see citing works
ON TISSUE ENGINEERING OF PIG, HUMAN, AND NON-HUMAN PRIMATE TISSUES
Nayakawde, N. B. (2020) · Gothenburg University Publications Electronic Archive (Gothenburg University) · doi:10.13140/rg.2.2.31294.72008 · Cited by 2 · see citing works
Waqanivavalagi, S. W. F. R., Ground, M., Alarcon, C., Milsom, P. F., & Cornish, J. (2021) · SSRN Electronic Journal · doi:10.2139/ssrn.3935488 · Cited by 2 · see citing works
Development of a 3D tumor model based on decellularized matrix using high-throughput approaches
Siahmansouri, H., Fenoglio, D., Filaci, G., & Mastrogiacomo, M. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1690844 · Cited by 1 · see citing works
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Self-citationEvaluation of Ultrasound Application for the Decellularization of Small Caliber Vessels
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Tissue Scaffolds Derived from Goat Aorta by Herbal Detergent
Khangembam, S. D., Gangwar, A. K., Goyal, R. P., Kumar, N., Kumar, V., Verma, M. K., Ahmed, P., & Yadav, V. K. (2022) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-2425-8_18 · Cited by 1 · see citing works
Decellularized Vascular Grafts
Toumpaniari, S., Hilfiker, A., Haverich, A., & Korossis, S. (2020) · Springer eBooks · doi:10.1007/978-3-319-71530-8_8-1 · Cited by 1 · see citing works
Extracellular Matrix-mediated Lung Epithelial Cell Differentiation
Shojaie, S. (2016) · TSpace (University of Toronto)
Yusof, F. M., Sharban, M., & Azhim, A. (2018) · IEEE-EMBS Conference on Biomedical Engineering and Sciences · doi:10.1109/iecbes.2018.8626689
Self-citationDevelopment of decellularised allogeneic nerve grafts
Webster, G. E. (2019) · White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)
Development of sterilisation strategies for decellularised peripheral nerve grafts
Holland, J. D. R. (2019) · White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)
Matrix Biology of the Salivary Gland: A Guide for Tissue Engineering
Martinez, M., Wu, D., Farach‐Carson, M. C., & Harrington, D. A. (2026) · Springer eBooks · doi:10.1007/978-3-032-07458-4_8
Preservation and Reuse of KLEx Lenticules: From Biobanking to Therapeutic and Refractive Innovations
Shoufani, R. C., Ferrari, S., Ponzin, D., Nubile, M., Riau, A. K., & Mehta, J. S. (2026) · Bioengineering · doi:10.3390/bioengineering13091075
- Authors
- Azhim, A., Syazwani, N., Morimoto, Y., Furukawa, K., & Ushida, T.
- Affiliations
- Center for Disease Biology and Integrative Medicine, The University of Tokyo (JP)
- University of Technology Malaysia (MY)
- National Defense Medical College Hospital (JP)
- The University of Tokyo (JP)
- Corresponding author
- Azhim, A
- Venue
- Journal of Biomaterials Applications
- Publisher
- SAGE Publications
- Published
- 2014-01-02
- Volume
- 29
- Issue
- 1
- Pages
- 130-141
- ISSN
- 0885-3282 (ISSN-L)
- 1530-8022
- OpenAlex
- W2080831186
- Cited by
- 59
- References
- 52
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 9 · 2021: 5 · 2020: 6 · 2019: 9 · 2018: 4 · 2017: 4 · 2016: 5 · 2015: 4
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomaterials Applications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- sonication, decellularization, aortic tissue, bioscaffolds, sodium dodecyl sulfate, cell membrane damage, xenograft implantation, inflammation
- MeSH terms
- Animals, Aorta, Thoracic, Biocompatible Materials, Biomechanical Phenomena, Cell Separation, Detergents, DNA, Extracellular Matrix, Materials Testing, Sodium Dodecyl Sulfate, Sonication, Transplantation, Heterologous, Rats, Wistar, Tissue Engineering, Sus scrofa, Rats, Tissue Scaffolds
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