Labile plasma iron in iron overload: redox activity and susceptibility to chelation
Open accessAbstract
Plasma non-transferrin-bound-iron (NTBI) is believed to be responsible for catalyzing the formation of reactive radicals in the circulation of iron overloaded subjects, resulting in accumulation of oxidation products. We assessed the redox active component of NTBI in the plasma of healthy and β-thalassemic patients. The labile plasma iron (LPI) was determined with the fluorogenic dihydrorhodamine 123 by monitoring the generation of reactive radicals prompted by ascorbate but blocked by iron chelators. The assay was LPI specific since it was generated by physiologic concentrations of ascorbate, involved no sample manipulation, and was blocked by iron chelators that bind iron selectively. LPI, essentially absent from sera of healthy individuals, was present in those of β-thalassemia patients at levels (1-16 μM) that correlated significantly with those of NTBI measured as mobilizer-dependent chelatable iron or desferrioxamine chelatable iron. Oral treatment of patients with deferiprone (L1) raised plasma NTBI due to iron mobilization but did not lead to LPI appearance, indicating that L1-chelated iron in plasma was not redox active. Moreover, oral L1 treatment eliminated LPI in patients. The approach enabled the assessment of LPI susceptibility to in vivo or in vitro chelation and the potential of LPI to cause tissue damage, as found in iron overload conditions. (Blood. 2003;102:2670-2677)
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- Authors
- Esposito, B. P., Breuer, W., Sirankapracha, P., Pootrakul, P., Hershko, C., & Cabantchik, Z. I.
- Affiliations
- From the Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Israel; Thalassemia Research Center, Institute of Science and Technology for Research and Development, Mahidol University, Salaya Campus, Nakornpathom, Thailand; and the Department of Hematology, Shaare Zedek Medical Center, Jerusalem, Israel.
- Hebrew University of Jerusalem (IL)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2003-10-01
- Volume
- 102
- Issue
- 7
- Pages
- 2670-2677
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2068337287
- Cited by
- 475
- References
- 40
- Citations by year
- 2026: 5 · 2025: 16 · 2024: 9 · 2023: 15 · 2022: 12 · 2021: 19 · 2020: 18 · 2019: 19 · 2018: 26 · 2017: 32 · 2016: 32 · 2015: 25 · 2014: 33 · 2013: 25 · 2012: 29
- FWCI
- 5.45
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hebrew University of Jerusalem
- National Research Council of Thailand
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Keywords
- labile plasma iron, non-transferrin-bound iron, iron overload, beta-thalassemia, redox activity, reactive oxygen radicals, iron chelation, deferiprone, dihydrorhodamine 123, vitamin C, iron mobilization, tissue damage
- MeSH terms
- Ascorbic Acid, Humans, Iron, Iron Chelating Agents, Oxidation-Reduction, Rhodamines, Thalassemia, Transferrin, Cohort Studies, Iron Overload
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