Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 246

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

Gambogic acid, a novel ligand for transferrin receptor, potentiates TNF-induced apoptosis through modulation of the nuclear factor-κB signaling pathway

Open access
Pandey, Manoj K., Sung, Bokyung, Ahn, Kwang Seok, Kunnumakkara, Ajaikumar B., Chaturvedi, Madan M., Aggarwal, Bharat B.·2007·Blood
DOI 10.1182/blood-2007-03-079616
Open this paper in CoChat — free account

Abstract

Gambogic acid (GA), a xanthone derived from the resin of the Garcinia hanburyi, has been recently demonstrated to bind transferrin receptor and exhibit potential anticancer effects through a signaling mechanism that is not fully understood. Because of the critical role of NF-κB signaling pathway, we investigated the effects of GA on NF-κB–mediated cellular responses and NF-κB–regulated gene products in human leukemia cancer cells. Treatment of cells with GA enhanced apoptosis induced by tumor necrosis factor (TNF) and chemotherapeutic agents, inhibited the expression of gene products involved in antiapoptosis (IAP1 and IAP2, Bcl-2, Bcl-xL, and TRAF1), proliferation (cyclin D1 and c-Myc), invasion (COX-2 and MMP-9), and angiogenesis (VEGF), all of which are known to be regulated by NF-κB. GA suppressed NF-κB activation induced by various inflammatory agents and carcinogens and this, accompanied by the inhibition of TAK1/TAB1-mediated IKK activation, inhibited IκBα phosphorylation and degradation, suppressed p65 phosphorylation and nuclear translocation, and finally abrogated NF-κB–dependent reporter gene expression. The NF-κB activation induced by TNFR1, TRADD, TRAF2, NIK, TAK1/TAB1, and IKKβ was also inhibited. The effect of GA mediated through transferrin receptor as down-regulation of the receptor by RNA interference reversed its effects on NF-κB and apoptosis. Overall our results demonstrate that GA inhibits NF-κB signaling pathway and potentiates apoptosis through its interaction with the transferrin receptor.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1182/blood-2007-03-079616

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 5 references

Research works printed but not linked: 52

  1. Aggarwal, 2006, From traditional Ayurvedic medicine to modern medicine: identification of therapeutic targets for suppression of inflammation and cancer., Expert Opin Ther Targets, 10, 87, 10.1517/14728222.10.1.87 · DOI · Paper page
  2. Guo, 2006, Inhibition of human telomerase reverse transcriptase gene expression by gambogic acid in human hepatoma SMMC-7721 cells., Life Sci, 78, 1238, 10.1016/j.lfs.2005.06.046 · DOI
  3. Zhang, 2004, Discovery, characterization and SAR of gambogic acid as a potent apoptosis inducer by a HTS assay., Bioorg Med Chem, 12, 309, 10.1016/j.bmc.2003.11.013 · DOI · Paper page
  4. Zhao, 2004, Gambogic acid induces apoptosis and regulates expressions of Bax and Bcl-2 protein in human gastric carcinoma MGC-803 cells., Biol Pharm Bull, 27, 998, 10.1248/bpb.27.998 · doi:10.1248/bpb.27.998
  5. Liu, 2005, Anticancer effect and apoptosis induction of gambogic acid in human gastric cancer line BGC-823., World J Gastroenterol, 11, 3655, 10.3748/wjg.v11.i24.3655 · DOI
  6. Yu, 2007, Gambogic acid-induced G2/M phase cell-cycle arrest via disturbing CDK7-mediated phosphorylation of CDC2/p34 in human gastric carcinoma BGC-823 cells., Carcinogenesis, 28, 632, 10.1093/carcin/bgl168 · doi:10.1093/carcin/bgl168
  7. Yu, 2006, Repression of telomerase reverse transcriptase mRNA and hTERT promoter by gambogic acid in human gastric carcinoma cells., Cancer Chemother Pharmacol, 58, 434, 10.1007/s00280-005-0177-2 · DOI
  8. Wu, 2004, Gambogic acid inhibits proliferation of human lung carcinoma SPC-A1 cells in vivo and in vitro and represses telomerase activity and telomerase reverse transcriptase mRNA expression in the cells., Biol Pharm Bull, 27, 1769, 10.1248/bpb.27.1769 · DOI · Paper page
  9. Kasibhatla, 2005, A role for transferrin receptor in triggering apoptosis when targeted with gambogic acid., Proc Natl Acad Sci U S A, 102, 12095, 10.1073/pnas.0406731102 · DOI · Paper page
  10. Ghosh, 1998, NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses., Annu Rev Immunol, 16, 225, 10.1146/annurev.immunol.16.1.225 · DOI · Paper page
  11. Aggarwal, 2004, Nuclear factor-kappaB: the enemy within., Cancer Cell, 6, 203, 10.1016/j.ccr.2004.09.003 · DOI · Paper page
  12. Anto, 2002, Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2., Carcinogenesis, 23, 1511, 10.1093/carcin/23.9.1511 · DOI · Paper page
  13. Blonska, 2005, TAK1 is recruited to the tumor necrosis factor-alpha (TNF-alpha) receptor 1 complex in a receptor-interacting protein (RIP)-dependent manner and cooperates with MEKK3 leading to NF-kappaB activation., J Biol Chem, 280, 43056, 10.1074/jbc.M507807200 · DOI
  14. Chaturvedi, 2000, Assay for redox-sensitive transcription factor., Methods Enzymol, 319, 585, 10.1016/S0076-6879(00)19055-X · DOI · Paper page
  15. Reddy, 1994, Reconstitution of nuclear factor kappa B activation induced by tumor necrosis factor requires membrane-associated components: comparison with pathway activated by ceramide., J Biol Chem, 269, 25369, 10.1016/S0021-9258(18)47258-4 · doi:10.1016/s0021-9258(18)47258-4
  16. Hu, 1999, Hematopoietic progenitor kinase-1 (HPK1) stress response signaling pathway activates IkappaB kinases (IKK-alpha/beta) and IKK-beta is a developmentally regulated protein kinase., Oncogene, 18, 5514, 10.1038/sj.onc.1202740 · DOI
  17. Darnay, 1999, Activation of NF-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif., J Biol Chem, 274, 7724, 10.1074/jbc.274.12.7724 · DOI · Paper page
  18. Bharti, 2004, Nuclear factor-kappaB and STAT3 are constitutively active in CD138+ cells derived from multiple myeloma patients, and suppression of these transcription factors leads to apoptosis., Blood, 103, 3175, 10.1182/blood-2003-06-2151 · DOI · Paper page
  19. Takada, 2004, Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis., J Biol Chem, 279, 15096, 10.1074/jbc.M311192200 · DOI · Paper page
  20. Kollia, 2001, Molecular analysis of transferrin receptor mRNA expression in acute myeloid leukemia., Br J Haematol, 115, 19, 10.1046/j.1365-2141.2001.03065.x · DOI
  21. Mayo, 1997, Requirement of NF-kappaB activation to suppress p53-independent apoptosis induced by oncogenic Ras., Science, 278, 1812, 10.1126/science.278.5344.1812 · DOI · Paper page
  22. Giri, 1998, Constitutive activation of NF-kappaB causes resistance to apoptosis in human cutaneous T cell lymphoma HuT-78 cells: autocrine role of tumor necrosis factor and reactive oxygen intermediates., J Biol Chem, 273, 14008, 10.1074/jbc.273.22.14008 · DOI · Paper page
  23. Wang, 1998, NF-kappaB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation., Science, 281, 1680, 10.1126/science.281.5383.1680 · DOI · Paper page
  24. You, 1997, ch-IAP1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein., Mol Cell Biol, 17, 7328, 10.1128/MCB.17.12.7328 · DOI · Paper page
  25. Chu, 1997, Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-kappaB control., Proc Natl Acad Sci U S A, 94, 10057, 10.1073/pnas.94.19.10057 · DOI · Paper page
  26. Tamatani, 1999, Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons., J Biol Chem, 274, 8531, 10.1074/jbc.274.13.8531 · DOI · Paper page
  27. Schwenzer, 1999, The human tumor necrosis factor (TNF) receptor-associated factor 1 gene (TRAF1) is up-regulated by cytokines of the TNF ligand family and modulates TNF-induced activation of NF-kappaB and c-Jun N-terminal kinase., J Biol Chem, 274, 19368, 10.1074/jbc.274.27.19368 · DOI · Paper page
  28. Dual, 1992, Tran activation of the c-myc promoter by human T cell leukemia virus type 1 tax is mediated by NF kappa B., J Biol Chem, 267, 16288, 10.1016/S0021-9258(18)41998-9 · DOI
  29. Guttridge, 1999, NF-kappaB controls cell growth and differentiation through transcriptional regulation of cyclin D1., Mol Cell Biol, 19, 5785, 10.1128/MCB.19.8.5785 · DOI · Paper page
  30. van de Stolpe, 1994, 12-O-tetradecanoylphorbol-13-acetate- and tumor necrosis factor alpha-mediated induction of intercellular adhesion molecule-1 is inhibited by dexamethasone: functional analysis of the human intercellular adhesion molecular-1 promoter., J Biol Chem, 269, 6185, 10.1016/S0021-9258(17)37586-5 · DOI · Paper page
  31. Chilov, 1997, Genomic organization of human and mouse genes for vascular endothelial growth factor C., J Biol Chem, 272, 25176, 10.1074/jbc.272.40.25176 · DOI · Paper page
  32. Esteve, 2002, Protein kinase C-zeta regulates transcription of the matrix metalloproteinase-9 gene induced by IL-1 and TNF-alpha in glioma cells via NF-kappa B., J Biol Chem, 277, 35150, 10.1074/jbc.M108600200 · DOI · Paper page
  33. Nelsen, 1988, The NF-kappa B-binding site mediates phorbol ester-inducible transcription in nonlymphoid cells., Mol Cell Biol, 8, 3526, 10.1128/MCB.8.8.3526 · DOI
  34. Osborn, 1989, Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B., Proc Natl Acad Sci U S A, 86, 2336, 10.1073/pnas.86.7.2336 · DOI · Paper page
  35. Sen, 1986, Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism., Cell, 47, 921, 10.1016/0092-8674(86)90807-X · DOI · Paper page
  36. Thevenin, 1990, Induction of nuclear factor-kappa B and the human immunodeficiency virus long terminal repeat by okadaic acid, a specific inhibitor of phosphatases 1 and 2A., New Biol, 2, 793
  37. Schreck, 1991, Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1., EMBO J, 10, 2247, 10.1002/j.1460-2075.1991.tb07761.x · DOI · Paper page
  38. Mahon, 1995, Studies into the effect of the tyrosine kinase inhibitor herbimycin A on NF-kappa B activation in T lymphocytes: evidence for covalent modification of the p50 subunit., J Biol Chem, 270, 28557, 10.1074/jbc.270.48.28557 · DOI
  39. Natarajan, 1996, Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B., Proc Natl Acad Sci U S A, 93, 9090, 10.1073/pnas.93.17.9090 · DOI · Paper page
  40. Finco, 1994, Inducible phosphorylation of I kappa B alpha is not sufficient for its dissociation from NF-kappa B is inhibited by protease inhibitors., Proc Natl Acad Sci U S A, 91, 11884, 10.1073/pnas.91.25.11884 · DOI · Paper page
  41. Sandur, 2006, Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherapeutic agents., J Biol Chem, 281, 17023, 10.1074/jbc.M601595200 · DOI · Paper page
  42. Campbell, 2004, Active repression of antiapoptotic gene expression by RelA(p65) NF-kappa B., Mol Cell, 13, 853, 10.1016/S1097-2765(04)00131-5 · DOI · Paper page
  43. Hsu, 1996, TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways., Cell, 84, 299, 10.1016/S0092-8674(00)80984-8 · DOI · Paper page
  44. Simeonidis, 1999, Mechanisms by which IkappaB proteins control NF-kappaB activity., Proc Natl Acad Sci U S A, 96, 49, 10.1073/pnas.96.1.49 · DOI
  45. Wang, 2001, TAK1 is a ubiquitin-dependent kinase of MKK and IKK., Nature, 412, 346, 10.1038/35085597 · DOI · Paper page
  46. Sizemore, 2002, Distinct roles of the Ikappa B kinase alpha and beta subunits in liberating nuclear factor kappa B (NF-kappa B) from Ikappa B and in phosphorylating the p65 subunit of NF-kappa B., J Biol Chem, 277, 3863, 10.1074/jbc.M110572200 · DOI · Paper page
  47. Ting, 1996, RIP mediates tumor necrosis factor receptor 1 activation of NF-kappaB but not Fas/APO-1-initiated apoptosis., EMBO J, 15, 6189, 10.1002/j.1460-2075.1996.tb01007.x · DOI · Paper page
  48. Kelliher, 1998, The death domain kinase RIP mediates the TNF-induced NF-kappaB signal., Immunity, 8, 297, 10.1016/S1074-7613(00)80535-X · DOI · Paper page
  49. Sakurai, 1999, Functional interactions of transforming growth factor beta-activated kinase 1 with IkappaB kinases to stimulate NF-kappaB activation., J Biol Chem, 274, 10641, 10.1074/jbc.274.15.10641 · DOI · Paper page
  50. Zhong, 1998, Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300., Mol Cell, 1, 661, 10.1016/S1097-2765(00)80066-0 · DOI · Paper page
  51. Tsuji, 1991, Tumor necrosis factor-alpha and interleukin 1-alpha regulate transferrin receptor in human diploid fibroblasts: relationship to the induction of ferritin heavy chain., J Biol Chem, 266, 7257, 10.1016/S0021-9258(20)89638-0 · DOI · Paper page
  52. Akiyama, 2003, Nuclear factor-kappaB p65 mediates tumor necrosis factor alpha-induced nuclear translocation of telomerase reverse transcriptase protein., Cancer Res, 63, 18

Cited by

See all 246

Metadata

Authors
Pandey, M. K., Sung, B., Ahn, K. S., Kunnumakkara, A. B., Chaturvedi, M. M., & Aggarwal, B. B.
Venue
Blood
Publisher
American Society of Hematology
Published
2007-11-15
Volume
110
Issue
10
Pages
3517-3525
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood-2007-03-079616
OpenAlex
W2034407136
Cited by
246
References
5
FWCI
5.25
Type
journal article
Language
en
Update notices
expression_of_concern (2013-05-02)
Open access
bronze

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Gambogic acid, a novel ligand for transferrin receptor, potentiates TNF-induced apoptosis through modulation of the nuclear factor-κB signaling pathway. https://app.cochat.ai/papers/10.1182/blood-2007-03-079616
The paper
Pandey, M. K., Sung, B., Ahn, K. S., Kunnumakkara, A. B., Chaturvedi, M. M., & Aggarwal, B. B. (2007). Gambogic acid, a novel ligand for transferrin receptor, potentiates TNF-induced apoptosis through modulation of the nuclear factor-κB signaling pathway. Blood. https://doi.org/10.1182/blood-2007-03-079616

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.