Cited by 1,210NF-κB Signaling PathwaysSignaling Pathways in DiseaseGenomics and Chromatin Dynamics

Phosphorylation of NF-κB p65 by PKA Stimulates Transcriptional Activity by Promoting a Novel Bivalent Interaction with the Coactivator CBP/p300

Open access
Zhong, Haihong, Voll, Reinhard E, Ghosh, Sankar·1998·Molecular Cell

Abstract

The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine 276. Accessibility to both sites is blocked in unphosphorylated p65 through an intramolecular masking of the N terminus by the C-terminal region of p65. Phosphorylation by PKA both weakens the interaction between the N- and C-terminal regions of p65 and creates an additional site for interaction with CBP/p300. Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300.

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Crossref · 10.1016/s1097-2765(00)80066-0

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Authors
Zhong, H., Voll, R. E., & Ghosh, S.
Affiliations
Howard Hughes Medical Institute (US)
Corresponding author
Ghosh, Sankar
Venue
Molecular Cell
Publisher
Elsevier BV
Published
1998-04
Volume
1
Issue
5
Pages
661-671
ISSN
1097-2765 (ISSN-L)
OpenAlex
W1985118687
Cited by
1,210
References
2
Citations by year
2026: 14 · 2025: 13 · 2024: 19 · 2023: 15 · 2022: 18 · 2021: 29 · 2020: 17 · 2019: 26 · 2018: 31 · 2017: 23 · 2016: 33 · 2015: 34 · 2014: 42 · 2013: 37 · 2012: 41
FWCI
25.89
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health (RO1 AI 33443)
  • Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
NF-κB p65, protein kinase A, CBP/p300, transcriptional coactivator, transcriptional regulation, protein-protein interaction, C-terminal domain
MeSH terms
p300-CBP-Associated Factor, Acetyltransferases, Amino Acid Sequence, DNA-Binding Proteins, Humans, Molecular Sequence Data, Nuclear Proteins, Peptide Fragments, Peptide Mapping, Phosphorylation, Promoter Regions, Genetic, Serine, Transcription Factors, Yeasts, Transcriptional Activation, Trans-Activators, NF-kappa B, Protein Structure, Tertiary, Cyclic AMP-Dependent Protein Kinases, Cell Cycle Proteins, Jurkat Cells, Saccharomyces cerevisiae Proteins, p300-CBP Transcription Factors, CREB-Binding Protein, Histone Acetyltransferases, Transcription Factor RelA, Nuclear Receptor Coactivator 3

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