Cited by 1,121Gut microbiota and healthRuminant Nutrition and Digestive PhysiologyAnimal Nutrition and Physiology

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Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)

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Dowd, Scot E, Callaway, Todd R, Wolcott, Randall D, Sun, Yan, McKeehan, Trevor, Hagevoort, Robert G, Edrington, Thomas S·2008·BMC Microbiology

DOI 10.1186/1471-2180-8-125PMC PMC2515157

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Abstract

BACKGROUND: The microbiota of an animal's intestinal tract plays important roles in the animal's overall health, productivity and well-being. There is still a scarcity of information on the microbial diversity in the gut of livestock species such as cattle. The primary reason for this lack of data relates to the expense of methods needed to generate such data. Here we have utilized a bacterial tag-encoded FLX 16s rDNA amplicon pyrosequencing (bTEFAP) approach that is able to perform diversity analyses of gastrointestinal populations. bTEFAP is relatively inexpensive in terms of both time and labor due to the implementation of a novel tag priming method and an efficient bioinformatics pipeline. We have evaluated the microbiome from the feces of 20 commercial, lactating dairy cows. RESULTS: Ubiquitous bacteria detected from the cattle feces included Clostridium, Bacteroides, Porpyhyromonas, Ruminococcus, Alistipes, Lachnospiraceae, Prevotella, Lachnospira, Enterococcus, Oscillospira, Cytophage, Anaerotruncus, and Acidaminococcus spp. Foodborne pathogenic bacteria were detected in several of the cattle, a total of 4 cows were found to be positive for Salmonella spp (tentative enterica) and 6 cows were positive for Campylobacter spp. (tentative lanienae). CONCLUSION: Using bTEFAP we have examined the microbiota in the feces of cattle. As these methods continue to mature we will better understand the ecology of the major populations of bacteria the lower intestinal tract. This in turn will allow for a better understanding of ways in which the intestinal microbiome contributes to animal health, productivity and wellbeing.

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Heinrichs, G., de Hoog, S., & Haase, G. (2012) · Journal of Clinical Microbiology · doi:10.1128/jcm.00574-12 · Cited by 38
The microbiome of neotropical ticks parasitizing on passerine migratory birds
Budachetri, K., Williams, J., Mukherjee, N., Sellers, M., Moore, F. R., & Karim, S. (2016) · Ticks and Tick-borne Diseases · doi:10.1016/j.ttbdis.2016.10.014 · Cited by 40
“Furthermore, the sequences with ambiguous base calls and homopolymer runs exceeding 6 base pairs in length were deleted (Acosta-Martinez et al., 2008; Dowd et al., 2011, 2008; Eren et al., 2011).”
Background
Differential expression of tumor-associated genes and altered gut microbiome with decreased Akkermansia muciniphila confer a tumor-preventive microenvironment in intestinal epithelial Pten-deficient mice
Howe, C. S., Kim, S. J., Mitchell, J., Im, E., Kim, Y. S., Kim, Y. S., & Rhee, S. H. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2018.10.006 · Cited by 38
“As described previously [26, 27], next generation technology (bTEFAP®) can be utilized to examine a broad range of microbiomes.”
Background
Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing
Jeong, J.-Y., Park, H., Lee, K.-H., Weon, H., & Ka, J.-O. (2011) · The Journal of Microbiology · doi:10.1007/s12275-011-0530-6 · Cited by 38
Smoking related environmental microbes affecting the pulmonary microbiome in Chinese population
Liu, X., Sun, W., Ma, W., Wang, H., Xu, K., Zhao, L., & He, Y. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.154652 · Cited by 36
Bacterial diversity of Drass, cold desert in Western Himalaya, and its comparison with Antarctic and Arctic
Gupta, P., Sangwan, N., Lal, R., & Vakhlu, J. (2015) · Archives of Microbiology · doi:10.1007/s00203-015-1121-4 · Cited by 37
“Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) (Dowd et al. 2008) was performed at the Research and Testing Laboratory, Lubbock, TX, USA (www.researchandtesting.com).”
BackgroundMethod
Necrotizing Enterocolitis and Cytomegalovirus Infection in a Premature Infant
Tran, L., Ferris, M. J., Norori, J., Stark, M. W., Craver, R. D., Dowd, S. E., & Penn, D. (2012) · PEDIATRICS · doi:10.1542/peds.2011-1971 · Cited by 37
Role of Diatoms in the Spatial-Temporal Distribution of Intracellular Nitrate in Intertidal Sediment
Stief, P., Kamp, A., & de Beer, D. (2013) · PLoS ONE · doi:10.1371/journal.pone.0073257 · Cited by 37
“PCR, sequencing, and initial quality checking were carried out as described in [32] at the Research and Testing Laboratory (RTL, Lubbock, TX, USA).”
Method
The ecological controls on the prevalence of candidate division TM7 in polar regions
Winsley, T. J., Snape, I., McKinlay, J. P., Stark, J., van Dorst, J. M., Ji, M., Ferrari, B. C., & Siciliano, S. D. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00345 · Cited by 37
“…were sent to a sequencing facility in Lubbock, Texas (Research and Testing Lab, Lubbock, TX, USA) for 16S rRNA gene amplicon sequencing using the PCR primers 27F and 519R (Lane, 1991) on the 454 FLX titanium platform as described by Dowd et al. (2008) (Roche Life Sciences, Branford, CT, USA).”
Method
Characterization of bacterial communities in wetland mesocosms receiving pharmaceutical-enriched wastewater
Zhang, D., Luo, J., Lee, Z. M. P., Maspolim, Y., Gersberg, R. M., Liu, Y., Tan, S. K., & Ng, W. J. (2016) · Ecological Engineering · doi:10.1016/j.ecoleng.2015.12.043 · Cited by 37
“The DNA samples were submitted to Research and Testing Laboratory (Lubbock, TX) for bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) (Dowd et al., 2008).”
Method
Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge
Hameed, S. A., Riffat, R., Li, B., Naz, I., Badshah, M., Ahmed, S., & Ali, N. (2019) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.5955 · Cited by 37
Microbially influenced corrosion of carbon steel in the presence of anaerobic sulphate-reducing bacteria
Černoušek, T., Shrestha, R., Kovářová, H., Špánek, R., Ševců, A., Sihelská, K., Kokinda, J., Stoulil, J., & Steinová, J. (2019) · Corrosion Engineering Science and Technology The International Journal of Corrosion Processes and Corrosion Control · doi:10.1080/1478422x.2019.1700642 · Cited by 37
“Amplification of the 16 S rDNA gene itself was undertaken using the primers 530F [43] and 802R [44].”
Method
Hydrocarbon-related microbial processes in the deep sediments of the Eastern Mediterranean Levantine Basin
Rubin‐Blum, M., Antler, G., Turchyn, A. V., Tsadok, R., Goodman‐Tchernov, B., Shemesh, E., Austin, J. A., Coleman, D. F., Makovsky, Y., Sivan, O., et al. (2013) · FEMS Microbiology Ecology · doi:10.1111/1574-6941.12264 · Cited by 38
Bacteriophage application restores ethanol fermentation characteristics disrupted by Lactobacillus fermentum
Liu, M., Bischoff, K. M., Gill, J. J., Mire-Criscione, M. D., Berry, J. D., Young, R., & Summer, E. J. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0325-9 · Cited by 37
“DNA was subject to bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) using primers 939F-TTGACGGGGGCCCGCAC and 1492R-TACCTTGTTACGACTT, and resulting sequences were analyzed as previously described [35, 36].”
Method
Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments
Bose, A., Rogers, D. R., Adams, M. M., Joye, S. B., & Girguis, P. R. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00386 · Cited by 38
“The extracted DNA was subjected to massively parallel sequencing of the 16S ribosomal RNA (rRNA) gene using Roche 454 Titanium™ chemistry and the primer pairs 27F/519R and 340F/806R for the bacterial V1–V3 and archaeal V3–V4 regions, respectively (Dowd et al., 2008; Acosta-Martinez et al., 2010).”
Method
Novel Culturing Techniques Select for Heterotrophs and Hydrocarbon Degraders in a Subantarctic Soil
van Dorst, J. M., Hince, G., Snape, I., & Ferrari, B. C. (2016) · Scientific Reports · doi:10.1038/srep36724 · Cited by 37
Microbial Diversity in Sulfate-Reducing Marine Sediment Enrichment Cultures Associated with Anaerobic Biotransformation of Coastal Stockpiled Phosphogypsum (Sfax, Tunisia)
Zouch, H., Karray, F., Armougom, F., Chifflet, S., Hirschler-Réa, A., Kharrat, H., Kamoun, L., Ben Hania, W., Ollivier, B., Sayadi, S., et al. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01583 · Cited by 37
“The mixtures of 16S rRNA gene amplicons were generated using a 341F/815R primer set, as previously described by Dowd et al. (2008), and were sequenced by the MiSeq Illumina (paired-end 2 × 300 bp) platform of the Molecular Research Laboratory (Texas, USA).”
Method
Partners to survive: Hoffmannseggia doellii root‐associated microbiome at the Atacama Desert
Maldonado, J. E., Gaete, A., Mandaković, D., Aguado‐Norese, C., Aguilar, M., Gutiérrez, R. A., & González, M. (2022) · New Phytologist · doi:10.1111/nph.18080 · Cited by 38
The effect of brown midrib corn silage and dried distillers' grains with solubles on milk production, nitrogen utilization and microbial community structure in dairy cows
Ramírez, H., NESTOR, K. E., Tedeschi, L. O., Callaway, T. R., Dowd, S. E., Fernando, S. C., & Kononoff, P. J. (2012) · Canadian Journal of Animal Science · doi:10.4141/cjas2011-133 · Cited by 36
Assessment of Bacterial Communities in Thirteen Species of Laboratory-Cultured Domestic Mites (Acari: Acaridida)
Hubert, J., Kopecký, J., Ságová‐Marečková, M., Nesvorná, M., Žůrek, L., & Erban, T. (2016) · Journal of Economic Entomology · doi:10.1093/jee/tow089 · Cited by 37
Mechanisms of Trichodesmium demise within the New Caledonian lagoon during the VAHINE mesocosm experiment
Spungin, D., Pfreundt, U., Berthelot, H., Bonnet, S., AlRoumi, D., Natale, F., Hess, W. R., Bidle, K. D., & Berman‐Frank, I. (2016) · Biogeosciences · doi:10.5194/bg-13-4187-2016 · Cited by 36
“The 16S rRNA genes within community genomic DNA were initially amplified with conserved bacterial primers 27F and 1100R (Dowd et al., 2008) using a high-fidelity polymerase (Phusion DNA polymerase, Thermo Scientific) with an initial denaturation step of 95 ◦C for 3 min followed by 20 cycles of 95…”
Method
Elimination of Naegleria fowleri from bulk water and biofilm in an operational drinking water distribution system
Miller, H. C., Morgan, M. J., Wylie, J. T., Kaksonen, A. H., Sutton, D. C., Braun, K., & Puzon, G. J. (2016) · Water Research · doi:10.1016/j.watres.2016.11.061 · Cited by 36
“…of biofilm samples (Table 2) at pre-RCl and post-RCl sites before and after the activation of the chlorinator were assessed by amplicon pyrosequencing of 16S rRNA genes using the total DNA extracted from a subset of field samples as previously described (Dowd et al., 2008; Morgan et al., 2016).”
Method
Microbiota Analysis of an Environmental Slurry and Its Potential Role as a Reservoir of Bovine Digital Dermatitis Pathogens
Schou, K. K., Strube, M. L., Isbrand, A., Jensen, T. K., & Nielsen, M. W. (2017) · Applied and Environmental Microbiology · doi:10.1128/aem.00244-17 · Cited by 36
“Most of these families are ubiquitously present in bovine rumen material or feces (8, 10, 16).”
Background
Stratification of chlorinated ethenes natural attenuation in an alluvial aquifer assessed by hydrochemical and biomolecular tools
Němeček, J., Dolinová, I., Macháčková, J., Špánek, R., Ševců, A., Lederer, T., & Černík, M. (2017) · Chemosphere · doi:10.1016/j.chemosphere.2017.06.100 · Cited by 36
Probiotic Yeast: Mode of Action and Its Effects on Ruminant Nutrition
Ghazanfar, S., Khalid, N., Ahmed, I., & Imran, M. (2017) · InTech eBooks · doi:10.5772/intechopen.70778 · Cited by 39
Different effects of nano-scale and micro-scale zero-valent iron particles on planktonic microorganisms from natural reservoir water
Nguyen, N. H. A., Špánek, R., Kasalický, V., Ribas, D. L., Vlková, D., Řeháková, H., Kejzlar, P., & Ševců, A. (2018) · Environmental Science Nano · doi:10.1039/c7en01120b · Cited by 36
Microbial effects of livestock manure fertilization on freshwater aquaculture ponds rearing tilapia (Oreochromis shiranus) and North African catfish (Clarias gariepinus)
Minich, J. J., Zhu, Q., Xu, Z. Z., Amir, A., Ngochera, M. J., Simwaka, M., Allen, E. E., Zidana, H., & Knight, R. D. (2018) · MicrobiologyOpen · doi:10.1002/mbo3.716 · Cited by 36
“Many animal gut associated microbes are anaerobic (Dowd et al., 2008), occupying a unique niche (Thompson et al., 2017) and thus may not be capable of surviving in an aerobic water environment.”
Background
Effects of lysophospholipids on short-term production, nitrogen utilization, and rumen fermentation and bacterial population in lactating dairy cows
Lee, C. H., Morris, D. L., Copelin, J. E., Hettick, J. M., & Kwon, I. (2019) · Journal of Dairy Science · doi:10.3168/jds.2018-15777 · Cited by 36
“Whole ruminal samples collected 4 h after feeding were analyzed for bacterial populations at Molecular Research DNA (Shallowater, TX) using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (Dowd et al., 2008; Hristov et al., 2012).”
Method
A Comparison of Two DNA Metagenomic Bioinformatic Pipelines While Evaluating the Microbial Diversity in Feces of Tanzanian Small Holder Dairy Cattle
Kibegwa, F. M., Bett, R. C., Gachuiri, C. K., Stomeo, F., & Mujibi, F. D. N. (2020) · BioMed Research International · doi:10.1155/2020/2348560 · Cited by 36
“abundance of these phototrophic oxygenic bacteria in dairy and beef cattle rumen [40, 41].”
Background
Porphyromonas spp. have an extensive host range in ill and healthy individuals and an unexpected environmental distribution: A systematic review and meta-analysis
Acuña-Amador, L., & Barloy-Hubler, F. (2020) · Anaerobe · doi:10.1016/j.anaerobe.2020.102280 · Cited by 35
Investigating the Role of Root Exudates in Recruiting Streptomyces Bacteria to the Arabidopsis thaliana Microbiome
Worsley, S. F., Macey, M. C., Prudence, S. M. M., Wilkinson, B. M., Murrell, J. C., & Hutchings, M. I. (2021) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.686110 · Cited by 36
Comparative Analysis of Fecal Microbiota of Grazing Mongolian Cattle from Different Regions in Inner Mongolia, China
Aricha, H., Simujide, H., Wang, C., Zhang, J., Lv, W., Jimisi, X., Liu, B., Chen, H., Zhang, C., He, L., et al. (2021) · Animals · doi:10.3390/ani11071938 · Cited by 36
Bovine Intestinal Bacteria Inactivate and Degrade Ceftiofur and Ceftriaxone with Multiple β-Lactamases
Wagner, R. D., Johnson, S. J., Cerniglia, C. E., & Erickson, B. D. (2011) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00008-11 · Cited by 36
“isolated in the present study are components of the normal bovine intestinal microbiota, and they are generally considered to be commensal organisms (14).”
Background
Assessment of biodegradation potential at a site contaminated by a mixture of BTEX, chlorinated pollutants and pharmaceuticals using passive sampling methods – Case study
Lhotský, O., Krákorová, E., Linhartová, L., Křesinová, Z., Steinová, J., Dvořák, L., Rødsand, T., Filipová, A., Kroupová, K., Wimmerová, L., et al. (2017) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2017.06.193 · Cited by 36
“Two consecutive PCR reactions per sample were performed to amplify the V4 region of the bacterial 16S rRNA gene using primers 530F (Dowd et al., 2008) and 802R (Claesson et al.”
Method
Evidence for Niche Partitioning Revealed by the Distribution of Sulfur Oxidation Genes Collected from Areas of a Terrestrial Sulfidic Spring with Differing Geochemical Conditions
Headd, B., & Engel, A. S. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.02812-12 · Cited by 35
“tag-encoded FLX amplicon pyrosequences (bTEFAP), as described previously (41, 42), with the forward primer 28F (5=-TTTGATCNTGGCTCA G-3=) and reverse primer 519r (5=-GTNTTACNGCGGCKGCTG-3=).”
Method
Anaerobic toxicity of cationic silver nanoparticles
Gitipour, A., Thiel, S. W., Scheckel, K. G., & Tolaymat, T. M. (2016) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2016.02.190 · Cited by 35
Prokaryotic and diazotrophic population dynamics within a large oligotrophic inverse estuary
Messer, L. F., Doubell, M. J., JEFFRIES, T. W., Brown, M., & Seymour, J. R. (2014) · Aquatic Microbial Ecology · doi:10.3354/ame01726 · Cited by 39
Gut microbiota may predict host divergence time during Glires evolution
Li, H., Qu, J., Li, T., Yao, M., Li, J., & Li, X. (2017) · FEMS Microbiology Ecology · doi:10.1093/femsec/fix009 · Cited by 34
Diversity and Activity of Diazotrophs in Great Barrier Reef Surface Waters
Messer, L. F., Brown, M. V., Furnas, M. J., Carney, R. L., McKinnon, A. D., & Seymour, J. R. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00967 · Cited by 35
“…Titanium; Molecular Research LP) after an additional 10 PCR cycles with custom barcoded nifH1 and nifH2 primers under the same reaction conditions (Dowd et al., 2008; Messer et al., 2015a), with between 5110 and 10860 sequences retrieved per sample (3561–7939 high quality sequences; Supplementary…”
Diversity and Persistence of the Gut Microbiome of the Giant Neotropical Bullet Ant
Moreau, C. S., & Rubin, B. E. R. (2017) · Integrative and Comparative Biology · doi:10.1093/icb/icx037 · Cited by 35
“Bacterial tag-encoded FLX titanium amplicon pyrosequencing (bTEFAP) was performed by the Research and Testing Laboratory (Lubbock, TX, USA) as described in Dowd et al. (2008).”
Method
Fecal microbiota of lambs fed purple prairie clover (Dalea purpurea Vent.) and alfalfa (Medicago sativa)
Huang, Q., Holman, D. B., Alexander, T. W., Hu, T., Jin, L., Xu, Z., McAllister, T. A., Acharya, S. N., Zhao, G., & Wang, Y. (2017) · Archives of Microbiology · doi:10.1007/s00203-017-1427-5 · Cited by 35
“2014) and therefore the large number of unclassified sequences within these families may explain why the proportion of sequences identified as Clostridium were relatively low compared to some reports in bovine feces (Callaway et al. 2010; Dowd et al. 2008).”
BackgroundResult
Facultative methanotrophs are abundant at terrestrial natural gas seeps
Haque, M. F. U., Crombie, A. T., Ensminger, S., Baciu, C. L., & Murrell, J. C. (2018) · Microbiome · doi:10.1186/s40168-018-0500-x · Cited by 35
Comparison of Bacterial Diversity in Air and Water of a Major Urban Center
Dueker, M. E., French, S. R., & O'Mullan, G. D. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02868 · Cited by 36
“Amplicon pyrosequencing was then performed on extracted environmental DNA at Molecular Research DNA labs (1MRDNA, Shallowater, TX, United States), using a protocol described by Dowd et al. (2008) to determine bacterial community composition.”
Method
Comparative study of vulva and abdominal skin microbiota of healthy females with high and average BMI
Vongsa, R., Hoffman, D., Shepard, K., & Koenig, D. W. (2019) · BMC Microbiology · doi:10.1186/s12866-019-1391-0 · Cited by 37
Gut Microbiome Perturbations in Patients with Hidradenitis Suppurativa: A Case Series
Kam, S. A., Collard, M., Lam, J., & Alani, R. M. (2020) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2020.04.017 · Cited by 35
CO2-induced shift in microbial activity affects carbon trapping and water quality in anoxic bioreactors
Kirk, M. F., Santillan, E. F. U., Sanford, R. A., & Altman, S. J. (2013) · Geochimica et Cosmochimica Acta · doi:10.1016/j.gca.2013.08.018 · Cited by 35
“After PCR, amplicon pyrosequencing 227 (bTEFAP) as described by Dowd et al. (2008) was used to sequence 16S rRNA genes.”
Method
The dynamics of biofilm bacterial communities is driven by flow wax and wane in a temporary stream
Timoner, X., Borrego, C., Acuña, V., & Sabater, S. (2014) · Limnology and Oceanography · doi:10.4319/lo.2014.59.6.2057 · Cited by 35
“Raw sequence data set was preprocessed at RTL facilities to reduce noise and sequencing artifacts as previously described (Dowd et al. 2008).”
Method
Biogeographic congruency among bacterial communities from terrestrial sulfidic springs
Headd, B., & Engel, A. S. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00473 · Cited by 35
“The V1-V3 region of the 16S rRNA gene was amplified and all sequencing was done by the Research and Testing Laboratories (Lubbock, Texas, USA), using previously described methods (Dowd et al., 2008a,b).”
Method
Subcutaneous Adipose Fatty Acid Profiles and Related Rumen Bacterial Populations of Steers Fed Red Clover or Grass Hay Diets Containing Flax or Sunflower-Seed
Petri, R. M., Mapiye, C., Dugan, M. E. R., & McAllister, T. A. (2014) · PLoS ONE · doi:10.1371/journal.pone.0104167 · Cited by 36
“Bacterial tag-encoded FLX-Titanium amplicon pyrosequencing (bTEFAP) of the pooled DNA, [23], was performed at MR DNA (Shallowater, TX, USA).”
Method
Distribution and microbial community structure analysis of a single-stage partial nitritation/anammox granular sludge bioreactor operating at low temperature
Rodríguez–Sánchez, A., Purswani, J., Lotti, T., Maza‐Márquez, P., van Loosdrecht, M. C. M., Vahala, R., & González‐Martínez, A. (2016) · Environmental Technology · doi:10.1080/09593330.2016.1147613 · Cited by 34
“Dowd et al.[17] by the Research & Testing Laboratory (Lubbock, TX, USA).”
Background
Bacterial community assemblages in sediments under high anthropogenic pressure at Ichkeul Lake/Bizerte Lagoon hydrological system, Tunisia
Salem, F. B., Saïd, O. B., Cravo‐Laureau, C., Mahmoudi, E., Bru, N., Monperrus, M., & Duran, R. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2019.05.146 · Cited by 37
Pubertal probiotic blocks LPS-induced anxiety and the associated neurochemical and microbial outcomes, in a sex dependent manner
Murray, E., Smith, K. B., Stoby, K. S., Thomas, B. J., Swenson, M. J., Arber, L., Frenette, E., & Ismail, N. (2019) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2019.104481 · Cited by 34

Authors: Dowd, S. E., Callaway, T. R., Wolcott, R. D., Sun, Y., McKeehan, T., Hagevoort, R. G., & Edrington, T. S.
Affiliations: Agricultural Research Service (US)
Corresponding author: Dowd, Scot E
ORCID: Dowd, Scot E
Callaway, Todd R
Edrington, Thomas S
Venue: BMC Microbiology
Publisher: Springer Science and Business Media LLC
Published: 2008
Volume: 8
Issue: 1
Pages: 125
ISSN: 1471-2180 (ISSN-L)
DOI: 10.1186/1471-2180-8-125
PMC: PMC2515157
OpenAlex: W2105807803
Cited by: 1,121
References: 80
Citations by year: 2026: 16 · 2025: 23 · 2024: 32 · 2023: 32 · 2022: 45 · 2021: 65 · 2020: 52 · 2019: 60 · 2018: 68 · 2017: 105 · 2016: 116 · 2015: 121 · 2014: 129 · 2013: 99 · 2012: 75
FWCI: 11.37
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: BMC Microbiology (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders: U.S. Department of Agriculture
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords: fecal microbiota, bacterial diversity, dairy cattle, Clostridium, Bacteroides, Ruminococcus, Lachnospiraceae, foodborne pathogens, Salmonella, Campylobacter, gut microbiota
MeSH terms: Animals, Bacteria, Cattle, DNA, Bacterial, DNA, Ribosomal, Feces, Female, RNA, Ribosomal, 16S, Polymerase Chain Reaction, Sequence Analysis, DNA, Gastrointestinal Tract, Biodiversity

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Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)

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