Cited by 112Fatty Acid Research and HealthAdipose Tissue and MetabolismMitochondrial Function and Pathology

Conjugated linoleic acid or omega 3 fatty acids increase mitochondrial biosynthesis and metabolism in skeletal muscle cells

Open access
Vaughan, Roger A, Garcia-Smith, Randi, Bisoffi, Marco, Conn, Carole A, Trujillo, Kristina A·2012·Lipids in Health and Disease

Abstract

BACKGROUND: Polyunsaturated fatty acids are popular dietary supplements advertised to contribute to weight loss by increasing fat metabolism in liver, but the effects on overall muscle metabolism are less established. We evaluated the effects of conjugated linoleic acid (CLA) or combination omega 3 on metabolic characteristics in muscle cells. METHODS: Human rhabdomyosarcoma cells were treated with either DMSO control, or CLA or combination omega 3 for 24 or 48 hours. RNA was determined using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Mitochondrial content was determined using flow cytometry and immunohistochemistry. Metabolism was quantified by measuring extracellular acidification and oxygen consumption rates. RESULTS: Omega 3 significantly induced metabolic genes as well as oxidative metabolism (oxygen consumption), glycolytic capacity (extracellular acidification), and metabolic rate compared with control. Both treatments significantly increased mitochondrial content. CONCLUSION: Omega 3 fatty acids appear to enhance glycolytic, oxidative, and total metabolism. Moreover, both omega 3 and CLA treatment significantly increase mitochondrial content compared with control.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/1476-511x-11-142

Cites

47 of 47 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • A human cell line study reported that omega-3 treatment significantly induced both glycolytic and oxidative metabolism and increased mitochondrial content.

    “Similarly, a human cell line study also reported that omega-3 treatment significantly induced both glycolytic and oxidative metabolism and increased mitochondrial content [92].”
  • A combination of EPA and DHA in rhabdomyosarcoma cells reduced the ratio between oxygen consumption rate and extracellular acidification rate, indicating a shift towards increased glycolytic reliance.

    “Furthermore, a combination of EPA and DHA in rhabdomyosarcoma cells reduced the ratio between the oxygen consumption rate: extracellular acidification rate indicating a shift towards an increase on glycolytic reliance [94].”

    From Omega-3 Fatty Acids and Skeletal Muscle Health (2015) · cites this paper as [94]

  • Multiple studies have observed an increase in GLUT4 expression.

    “Multiple studies have observed an increase in GLUT4 expression [93,94]”

    From Omega-3 Fatty Acids and Skeletal Muscle Health (2015) · cites this paper as [94]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 48 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Vaughan, R. A., Garcia-Smith, R., Bisoffi, M., Conn, C. A., & Trujillo, K. A.
Affiliations
University of New Mexico (US)
Corresponding author
Vaughan, Roger A
Venue
Lipids in Health and Disease
Published
2012-10-30
Volume
11
Issue
1
Pages
142-142
ISSN
1476-511X (ISSN-L)
OpenAlex
W2117258334
Cited by
112
References
48
Citations by year
2026: 1 · 2025: 7 · 2024: 4 · 2023: 9 · 2022: 8 · 2021: 6 · 2020: 11 · 2019: 5 · 2018: 9 · 2017: 10 · 2016: 13 · 2015: 12 · 2014: 10 · 2013: 6 · 2012: 1
FWCI
8.66
Type
journal article
Language
en
Open access
Locations
  • Lipids in Health and Disease (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Keywords
conjugated linoleic acid, omega-3 fatty acids, mitochondrial biogenesis, skeletal muscle metabolism, rhabdomyosarcoma cells, glycolysis, oxidative metabolism, extracellular acidification, oxygen consumption, polyunsaturated fatty acids, dietary supplements
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Base Sequence, DNA, Fibronectins, Glycolysis, Heat-Shock Proteins, Humans, Mitochondria, Muscle, Oxygen Consumption, Transcription Factors, Tumor Cells, Cultured, Fatty Acids, Omega-3, Gene Expression, Muscle Fibers, Skeletal, Dietary Supplements, Linoleic Acids, Conjugated, Glucose Transporter Type 4

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review