Copulation or sensory cues from the female augment Fos expression in arginine vasopressin neurons of the posterodorsal medial amygdala of male rats
Open accessAbstract
BACKGROUND: The posterodorsal part of the medial amygdala is essential for processing reproductively salient sensory information in rodents. This is the initial brain structure where information from olfactory system and male hormones intersect. The neurochemical identity of the neurons participating in the sensory processing in medial amygdala remains presently undetermined. Many neurons in this brain structure express arginine vasopressin in a testosterone-dependent manner, suggesting that this neuropeptide is maintained by the androgenic milieu. METHOD: Here we use Fos, a protein expressed by recently active neurons, to quantify activation of arginine vasopressin neurons after exposure to odor from physically inaccessible female. We compare it to mating with accessible female and to reproductively innocuous odor. RESULTS: We show that inaccessible female activate arginine vasopressin neurons in the male posterodorsal medial amygdala. The magnitude of activation is not further enhanced when physical access with resultant mating is granted, even though it remains undetermined if same population of AVP neurons is activated by both inaccessible female and copulation. We also show that arginine vasopressin activation cannot be fully accounted for by mere increase in the number of Fos and AVP neurons. CONCLUSION: These observations posit a role for the medial amygdala arginine vasopressin in reproductive behaviors, suggesting that these neurons serve as integrative node between the hormonal status of the animal and the availability of reproductive opportunities.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Copulation with a female augments Fos expression in AVP-ir neurons in the MeP and BNST of adult male rats and mice.
“In line with this, copulation with a female augments Fos expression in AVP-ir neurons in the MeP and BNST of adult male rats (Hari Dass & Vyas, 2014) and mice (Ho et al., 2010).”
From Quantitative mapping reveals age and sex differences in vasopressin, but not oxytocin, immunoreactivity in the rat social behavior neural network (2017) · cites this paper as Hari Dass & Vyas, 2014
Table 1 in Results and discussion shows a higher percentage of Fos-positive AVP-ir neurons in the MePD after copulation in male rats. The paper does not report that finding for the rat BNST; its discussion mentions BNST activation during copulation in mice, which the citing sentence attributes separately to Ho et al. (2010). (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Functional identification of an aggression locus in the mouse hypothalamus
Lin, D., Boyle, M. P., Dollar, P., Lee, H., Lein, E. S., Perona, P., & Anderson, D. J. (2011) · Nature · doi:10.1038/nature09736 · Cited by 914 · see citing works
c-Fos and Related Immediate Early Gene Products as Markers of Activity in Neuroendocrine Systems
Hoffman, G. E., Smith, M. S., & Verbalis, J. G. (1993) · Frontiers in Neuroendocrinology · doi:10.1006/frne.1993.1006 · Cited by 715 · see citing works
Further contributions to the study of the evolution of the forebrain
Johnston, J. B. (1923) · The Journal of Comparative Neurology · doi:10.1002/cne.900350502 · Cited by 667 · see citing works
The vasopressinergic innervation of the brain in normal and castrated rats
DeVries, G. J., Buijs, R. M., van Leeuwen, F. W., Caffé, A. R., & Swaab, D. F. (1985) · Journal of Comparative Neurology · doi:10.1002/cne.902330206 · Cited by 522 · see citing works
Young, L. J., Nilsen, R., Waymire, K. G., MacGregor, G. R., & Insel, T. R. (1999) · Nature · doi:10.1038/23475 · Cited by 488 · see citing works
Subordination stress: Behavioral, brain, and neuroendocrine correlates
Blanchard, D. C., Sakai, R. R., McEwen, B. S., Weiss, S. Μ., & Blanchard, R. J. (1993) · Behavioural Brain Research · doi:10.1016/0166-4328(93)90096-9 · Cited by 484 · see citing works
Choi, G. B., Dong, H.-W., Murphy, A. J., Valenzuela, D. M., Yancopoulos, G. D., Swanson, L. W., & Anderson, D. J. (2005) · Neuron · doi:10.1016/j.neuron.2005.04.011 · Cited by 462 · see citing works
Young (1999) · doi:10.1038/23650 · Cited by 389 · see citing works
A brain sexual dimorphism controlled by adult circulating androgens
Cooke, B. M., Tabibnia, G., & Breedlove, S. M. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.13.7538 · Cited by 311 · see citing works
Medial Nucleus of the Amygdala Mediates Chemosensory Control of Male Hamster Sexual Behavior
Lehman, M. N., Winans, S. S., & Powers, J. B. (1980) · Science · doi:10.1126/science.7423209 · Cited by 282 · see citing works
Oxytocin/vasopressin-related peptides have an ancient role in reproductive behavior
Garrison, J. L., Macosko, E. Z., Bernstein, S., Pokala, N., Albrecht, D. R., & Bargmann, C. I. (2012) · Science · doi:10.1126/science.1226201 · Cited by 266 · see citing works
An intrinsic vasopressin system in the olfactory bulb is involved in social recognition
Tobin, V. A., Hashimoto, H., Wacker, D. W., Takayanagi, Y., Langnaese, K., Caquineau, C., Noack, J., Landgraf, R., Onaka, T., Leng, G., et al. (2010) · Nature · doi:10.1038/nature08826 · Cited by 213 · see citing works
Zhou, L., Blaustein, J. D., & De Vries, G. J. (1994) · Endocrinology · doi:10.1210/endo.134.6.8194487 · Cited by 189 · see citing works
Sexual difference in nuclear volume and its ontogeny in the rat amygdala
Mizukami, S., Nishizuka, M., & Arai, Y. (1983) · Experimental Neurology · doi:10.1016/0014-4886(83)90235-2 · Cited by 178 · see citing works
Dluzen, D. E., Muraoka, S., Engelmann, M., & Landgraf, R. (1998) · Peptides · doi:10.1016/s0196-9781(98)00047-3 · Cited by 169 · see citing works
Androgen-dependent vasopressinergic neurons are involved in social recognition in rats
Bluthe, R.-M., Schoenen, J., & Dantzer, R. (1990) · Brain Research · doi:10.1016/0006-8993(90)90073-k · Cited by 164 · see citing works
Kondo, Y. (1992) · Physiology & Behavior · doi:10.1016/0031-9384(92)90074-c · Cited by 163 · see citing works
Bressler, S., & Baum, M. J. (1996) · Neuroscience · doi:10.1016/0306-4522(95)00493-9 · Cited by 156 · see citing works
Evolving nonapeptide mechanisms of gregariousness and social diversity in birds
Goodson, J. L., Kelly, A. M., & Kingsbury, M. A. (2012) · Hormones and Behavior · doi:10.1016/j.yhbeh.2012.01.005 · Cited by 139 · see citing works
Cooke, B., Breedlove, S., & Jordan, C. L. (2003) · Hormones and Behavior · doi:10.1016/s0018-506x(02)00047-8 · Cited by 132 · see citing works
Integration of chemosensory and hormonal cues is essential for mating in the male Syrian hamster
Wood, R., & Newman, S. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-11-07261.1995 · Cited by 122 · see citing works
Vomeronasal, Olfactory, Hormonal Convergence in the Brain: Cooperation or Coincidence? <sup>a</sup>
MEREDITH, M. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb10593.x · Cited by 121 · see citing works
Auger, C. J., Coss, D., Auger, A. P., & Forbes‐Lorman, R. M. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100314108 · Cited by 119 · see citing works
Kondo, Y., Sachs, B. D., & Sakuma, Y. (1997) · Behavioural Brain Research · doi:10.1016/s0166-4328(97)02287-0 · Cited by 113 · see citing works
Greco, B. M., Edwards, D. A., Michael, R. P., & Clancy, A. N. (1998) · Neuroendocrinology · doi:10.1159/000054294 · Cited by 112 · see citing works
Vasopressin, oxytocin, and social odor recognition
Wacker, D. W., & Ludwig, M. (2012) · Hormones and Behavior · doi:10.1016/j.yhbeh.2011.08.014 · Cited by 112 · see citing works
Baum, M. J., Tobet, S. A., Starr, M. S., & Bradshaw, W. G. (1982) · Hormones and Behavior · doi:10.1016/0018-506x(82)90020-4 · Cited by 111 · see citing works
Steroid-dependent plasticity in the medial amygdala
Cooke, B. M. (2005) · Neuroscience · doi:10.1016/j.neuroscience.2005.06.018 · Cited by 108 · see citing works
Wood, R. I., & Coolen, L. M. (1997) · Neuroscience · doi:10.1016/s0306-4522(96)00629-x · Cited by 102 · see citing works
Kondo, Y., & Arai, Y. (1995) · Physiology & Behavior · doi:10.1016/0031-9384(94)00205-j · Cited by 92 · see citing works
Paredes, R. G., Lopez, M., & Baum, M. J. (1998) · Hormones and Behavior · doi:10.1006/hbeh.1998.1435 · Cited by 90 · see citing works
The Role of Vasopressin in the Genetic and Neural Regulation of Monogamy
Lim, M. M., Hammock, E. A. D., & Young, L. J. (2004) · Journal of Neuroendocrinology · doi:10.1111/j.0953-8194.2004.01162.x · Cited by 83 · see citing works
Wang, Z., & De Vries, G. J. (1995) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.1995.tb00722.x · Cited by 77 · see citing works
Ho, J. M., Murray, J. H., Demas, G. E., & Goodson, J. L. (2010) · Hormones and Behavior · doi:10.1016/j.yhbeh.2010.03.021 · Cited by 76 · see citing works
Maras, P. M., & Petrulis, A. (2006) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2006.05216.x · Cited by 72 · see citing works
Cooke, B. M., Chowanadisai, W., & Breedlove, S. M. (2000) · Behavioural Brain Research · doi:10.1016/s0166-4328(00)00301-6 · Cited by 67 · see citing works
Heeb, M. M., & Yahr, P. (2000) · Physiology & Behavior · doi:10.1016/s0031-9384(99)00182-1 · Cited by 66 · see citing works
Samuelsen, C. L., & Meredith, M. J. (2009) · Neuroscience · doi:10.1016/j.neuroscience.2009.09.030 · Cited by 58 · see citing works
Sexual dimorphism and steroid responsiveness of the posterodorsal medial amygdala in adult mice
Morris, J. A., Jordan, C. L., King, Z. A., Northcutt, K. V., & Breedlove, S. M. (2007) · Brain Research · doi:10.1016/j.brainres.2007.11.005 · Cited by 56 · see citing works
Androgen Implants in Medial Amygdala Briefly Maintain Noncontact Erection in Castrated Male Rats
Biały, M., & Sachs, B. D. (2002) · Hormones and Behavior · doi:10.1006/hbeh.2002.1821 · Cited by 52 · see citing works
Been, L. E., & Petrulis, A. (2011) · Hormones and Behavior · doi:10.1016/j.yhbeh.2011.02.005 · Cited by 47 · see citing works
Maras, P. M., & Petrulis, A. (2010) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2010.07289.x · Cited by 44 · see citing works
Forebrain Fos responses to reproductively related chemosensory cues in aromatase knockout mice
Aste, N., Honda, S., & Harada, N. (2003) · Brain Research Bulletin · doi:10.1016/s0361-9230(03)00035-2 · Cited by 39 · see citing works
Kabelik, D., Morrison, J. A., & Goodson, J. L. (2010) · Brain Behavior and Evolution · doi:10.1159/000297522 · Cited by 33 · see citing works
Blake, C. B., & Meredith, M. J. (2011) · Neuroscience · doi:10.1016/j.neuroscience.2011.05.056 · Cited by 25 · see citing works
Blockade of androgen receptor in the medial amygdala inhibits noncontact erections in male rats
Biały, M., Nikolaev-Diak, A., Kalata, U., & Nikolaev, E. (2011) · Physiology & Behavior · doi:10.1016/j.physbeh.2011.02.003 · Cited by 23 · see citing works
Impact of Social Conflict between Mice on Testosterone Binding in the Central Nervous System
Raab, A., & Haedenkamp, G. (2008) · Neuroendocrinology · doi:10.1159/000123172 · Cited by 19 · see citing works
He, F., Wu, R., & Yu, P. (2013) · Behavioural Brain Research · doi:10.1016/j.bbr.2013.10.004 · Cited by 17 · see citing works
van Zwieten, E. J., Kos, W., Ravid, R., & Swaab, D. F. (1993) · Neurobiology of Aging · doi:10.1016/0197-4580(93)90008-y · Cited by 11 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Dass, S. A. H., & Vyas, A. (2014) · Molecular Ecology · doi:10.1111/mec.12888 · Cited by 145 · see citing works
Self-citationDivergent medial amygdala projections regulate approach–avoidance conflict behavior
Miller, S. M., Marcotulli, D., Shen, A., & Zweifel, L. S. (2019) · Nature Neuroscience · doi:10.1038/s41593-019-0337-z · Cited by 112 · see citing works
Cádiz‐Moretti, B., Otero‐García, M., Martínez‐Garciá, F., & Lanuza, E. (2014) · Brain Structure and Function · doi:10.1007/s00429-014-0954-y · Cited by 88 · see citing works
Mechanisms of Host Behavioral Change in Toxoplasma gondii Rodent Association
Vyas, A. (2015) · PLoS Pathogens · doi:10.1371/journal.ppat.1004935 · Cited by 83 · see citing works
Self-citationDiBenedictis, B. T., Nussbaum, E. R., Cheung, H. K., & Veenema, A. H. (2017) · Journal of Comparative Neurology · doi:10.1002/cne.24216 · Cited by 74 · see citing works
“In line with this, copulation with a female augments Fos expression in AVP-ir neurons in the MeP and BNST of adult male rats (Hari Dass & Vyas, 2014) and mice (Ho et al., 2010).”
BackgroundKisspeptin in the medial amygdala and sexual behavior in male rats
Gresham, R., Li, S., Adekunbi, D. A., Hu, M., Li, X. F., & O’Byrne, K. T. (2016) · Neuroscience Letters · doi:10.1016/j.neulet.2016.05.042 · Cited by 68 · see citing works
Modulation of social behavior by distinct vasopressin sources
Rigney, N., Vries, G. J. D., & Petrulis, A. (2023) · Frontiers in Endocrinology · doi:10.3389/fendo.2023.1127792 · Cited by 61 · see citing works
Tong, W. H., Abdulai-Saiku, S., & Vyas, A. (2019) · Frontiers in Behavioral Neuroscience · doi:10.3389/fnbeh.2019.00033 · Cited by 41 · see citing works
Self-citationRigney, N., Zbib, A., de Vries, G. J., & Petrulis, A. (2022) · Journal of Neuroendocrinology · doi:10.1111/jne.13083 · Cited by 29 · see citing works
Tan, D., Soh, L. J. T., Lim, L. W., Daniel, T. C. W., Zhang, X., & Vyas, A. (2015) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.2015.0042 · Cited by 27 · see citing works
Self-citationAbdulai-Saiku, S., & Vyas, A. (2017) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2017.04.005 · Cited by 27 · see citing works
Self-citationMedial Amygdala Arginine Vasopressin Neurons Regulate Innate Aversion to Cat Odors in Male Mice
Tong, W. H., Abdulai-Saiku, S., & Vyas, A. (2020) · Neuroendocrinology · doi:10.1159/000508862 · Cited by 25 · see citing works
Self-citationZancan, M., Dall’Oglio, A., Quagliotto, É., & Rasia‐Filho, A. A. (2016) · European Journal of Neuroscience · doi:10.1111/ejn.13460 · Cited by 24 · see citing works
Neuroanatomical dichotomy of sexual behaviors in rodents
Angoa‐Pérez, M., & Kuhn, D. M. (2015) · Behavioural Pharmacology · doi:10.1097/fbp.0000000000000157 · Cited by 24 · see citing works
Hull, E. M., & Rodrı́guez–Manzo, G. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-803592-4.00001-8 · Cited by 24 · see citing works
Petersen, C. L., & Hurley, L. M. (2017) · Integrative and Comparative Biology · doi:10.1093/icb/icx055 · Cited by 23 · see citing works
Hodges, T. E., Louth, E. L., Bailey, C. D. C., & McCormick, C. M. (2018) · Brain Structure and Function · doi:10.1007/s00429-018-1789-8 · Cited by 22 · see citing works
Vasopressin Proves Essential: Vasopressin and the Modulation of Sensory Processing in Mammals
Bester‐Meredith, J. K., Fancher, A. P., & Mammarella, G. E. (2015) · Frontiers in Endocrinology · doi:10.3389/fendo.2015.00005 · Cited by 23 · see citing works
DiBenedictis, B. T., Cheung, H. K., Nussbaum, E. R., & Veenema, A. H. (2019) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2019.104462 · Cited by 20 · see citing works
Xu, L., Liu, T., He, Z., Hong, Q., & Xiang, H. (2015) · Epilepsy & Behavior · doi:10.1016/j.yebeh.2015.02.034 · Cited by 19 · see citing works
Behavioral Manipulation by Toxoplasma gondii: Does Brain Residence Matter?
Abdulai-Saiku, S., Tong, W. H., & Vyas, A. (2021) · Trends in Parasitology · doi:10.1016/j.pt.2020.12.006 · Cited by 17 · see citing works
Self-citationKelly, A. M., & Seifert, A. W. (2021) · Neuroscience · doi:10.1016/j.neuroscience.2021.05.034 · Cited by 17 · see citing works
Liu, T., He, Z., Tian, X., Liu, C., Xiang, H., & Zhang, J. (2014) · Epilepsy & Behavior · doi:10.1016/j.yebeh.2014.10.028 · Cited by 16 · see citing works
Tong, W. H., Abdulai-Saiku, S., & Vyas, A. (2021) · Molecular Brain · doi:10.1186/s13041-021-00850-2 · Cited by 12 · see citing works
Self-citationCsabafi, K., Ibos, K. E., Bodnár, É., Filkor, K., Szakács, J., & Bagosi, Z. (2023) · Biomedicines · doi:10.3390/biomedicines11092446 · Cited by 12 · see citing works
Extended epigenotype in aRattus novergicus–Toxoplasma gondiiassociation
Vyas, A. (2015) · Communicative & Integrative Biology · doi:10.4161/19420889.2014.992743 · Cited by 8 · see citing works
Self-citationAdolescent exposure to bisphenol-a antagonizes androgen regulation of social behavior in male mice
Zhong, X., Li, J., & Xu, X. (2024) · Neurotoxicology and Teratology · doi:10.1016/j.ntt.2024.107374 · Cited by 5 · see citing works
Borrow, A. P., Stover, S. A., Bales, N. J., & Handa, R. J. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-813814-4.00010-9 · Cited by 5 · see citing works
Arakawa, H., Tokashiki, M., Higuchi, Y., & Konno, T. (2024) · Peptides · doi:10.1016/j.peptides.2024.171178 · Cited by 4 · see citing works
Larios, B. C., & Elias, C. F. (2023) · Frontiers in Neuroanatomy · doi:10.3389/fnana.2022.1057727 · Cited by 4 · see citing works
The Basolateral Nucleus in the System of Reproductive Centers in the Amygdaloid Body of the Brain
Ахмадеев, А. В., Lf, G., & Калимуллина, Л. Б. (2016) · Neuroscience and Behavioral Physiology · doi:10.1007/s11055-016-0292-z · Cited by 1 · see citing works
dos Santos, S. V., Fortuna, G. D. L. S., Barbosa, L. M., Meireles, L. R., Tiago, É. S., & Chieffi, P. P. (2022) · Revista da Sociedade Brasileira de Medicina Tropical · doi:10.1590/0037-8682-0122-2022 · Cited by 1 · see citing works
An extended epiphenotype for an extended phenotype in Toxoplasma gondii infected feral house mice
Ngo, P., Lignereux, L., O'Handley, R. M., & Vyas, A. (2022) · Functional Ecology · doi:10.1111/1365-2435.14223 · Cited by 1 · see citing works
Self-citationCross-talk between the Oxytocin and Vasopressin Systems in the Brian: Roles in Social Behavior
Song, Z. (2022) · Digital Archive @ GSU · doi:10.57709/7996120
Ахмадеев, А. В., & Калимуллина, Л. Б. (2016) · Neuroscience and Behavioral Physiology · doi:10.1007/s11055-016-0316-8
He, F., Yan, B., Tian, Z., Wang, B., Cheng, X.-X., Wang, Z., & Yu, B. (2022) · European Journal of Neuroscience · doi:10.1111/ejn.15793
Tong, W. H., Abdulai-Saiku, S., & Vyas, A. (2021) · Figshare · doi:10.6084/m9.figshare.16625611
Self-citationTong, W. H., Abdulai-Saiku, S., & Vyas, A. (2021) · Figshare · doi:10.6084/m9.figshare.16625614
Self-citation
- Authors
- Hari Dass, S., & Vyas, A.
- Affiliations
- Nanyang Technological University (SG)
- Corresponding author
- Vyas, Ajai
- ORCID
- Vyas, Ajai
- Venue
- Frontiers in Zoology
- Published
- 2014
- Volume
- 11
- Issue
- 1
- Pages
- 42
- ISSN
- 1742-9994 (ISSN-L)
- PMC
- PMC4054915
- OpenAlex
- W2105344222
- Cited by
- 41
- References
- 49
- Citations by year
- 2024: 2 · 2023: 3 · 2022: 4 · 2021: 6 · 2020: 1 · 2019: 5 · 2018: 1 · 2017: 3 · 2016: 6 · 2015: 7 · 2014: 3
- FWCI
- 9.40
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Zoology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DR-NTU (Nanyang Technological University) (submittedVersion, cc-by)
- Funders
- Ministry of Education - Singapore
- Nanyang Technological University
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- vasopressin, posterodorsal medial amygdala, Fos expression, copulation, olfactory cues, androgen dependence, testosterone, reproductive behavior, male rats, sensory processing, neuronal activation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
