Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men

Hamer, Henrike M; Wall, Benjamin T; Kiskini, Alexandra; de Lange, Anneke; Groen, Bart B L; Bakker, Jaap A; Gijsen, Annemie P; Verdijk, Lex B; van Loon, Luc J C

doi:10.1186/1743-7075-10-15

Cited by 44Muscle metabolism and nutritionNutrition and Health in AgingMuscle Physiology and Disorders

Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men

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Hamer, Henrike M, Wall, Benjamin T, Kiskini, Alexandra, de Lange, Anneke, Groen, Bart B L, Bakker, Jaap A, Gijsen, Annemie P, Verdijk, Lex B +1 more·2013·Nutrition & Metabolism

DOI 10.1186/1743-7075-10-15PMC PMC3585863

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Abstract

Background: A blunted muscle protein synthetic response to protein ingestion may contribute to the age related loss of muscle tissue. We hypothesized that the greater endogenous insulin release following co-ingestion of carbohydrate facilitates post-prandial muscle protein accretion after ingesting a meal-like bolus of protein in older males. Methods: Twenty-four healthy older men (75±1 y) were randomly assigned to ingest 20 g intrinsically L-[1-13 C] phenylalanine-labeled casein protein with (PRO-CHO) or without (PRO) 40 g carbohydrate. Ingestion of specifically produced intrinsically L-[1-13 C] phenylalanine labeled protein allowed us to assess post-prandial incorporation of dietary protein derived amino acids into muscle protein. Blood samples were collected at regular intervals, with muscle biopsies being obtained prior to and 2 and 6 h after protein ingestion. Results: Plasma glucose and insulin concentrations showed a greater increase in PRO-CHO compared with PRO (P<0.001). Muscle protein-bound L-[1-13 C] phenylalanine enrichments tended to increase to a greater extent in PRO-CHO compared with PRO during the first 2 h after protein ingestion (0.0072±0.0013 vs 0.0046±0.010 MPE, respectively; P=0.13). However, 6 h after protein ingestion, differences in muscle protein-bound L-[1-13 C] phenylalanine enrichments were no longer observed between experiments (0.0213±0.0024 vs 0.0185±0.0010 MPE, respectively; P=0.30). Conclusions: This study shows that carbohydrate ingestion may accelerate, but does not further augment post-prandial incorporation of dietary protein derived amino acids into muscle protein in healthy elderly men.

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Adding a non-pharmacological dose of carbohydrates to a protein synthesis activating dose of proteins has no additive effect on FSR.
“Regardless of potential differences in insulinemia, adding a non‐pharmacological dose of carbohydrates to a protein synthesis activating dose of proteins (15–30 g) has no additive effect on FSR (Glynn et al., 2013; Hamer et al., 2013; Koopman et al., 2007; Staples et al., 2011), our primary outcome.”
From The effect of acute sleep deprivation on skeletal muscle protein synthesis and the hormonal environment (2021) · cites this paper as Hamer et al., 2013
The Results report no difference in six-hour muscle protein FSR between the protein-plus-carbohydrate and protein-only groups. The Discussion specifies that the study tested 20 g protein with or without 40 g carbohydrate in healthy older men; it does not establish the result across the full 15–30 g protein range or other populations. (LLM, conf=0.84)
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Homeostasis model assessment: insulin resistance and ?-cell function from fasting plasma glucose and insulin concentrations in man
Matthews, D. R., Hosker, J. P., Rudenski, A. S., Naylor, B. A., Treacher, D. F., & Turner, R. C. (1985) · Diabetologia · doi:10.1007/bf00280883 · Cited by 31,502 · see citing works
Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle
Cuthbertson, D., Smith, K., Babraj, J., Leese, G., Waddell, T., Atherton, P., Wackerhage, H., Taylor, P. M., & Rennie, M. J. (2005) · The FASEB Journal · doi:10.1096/fj.04-2640fje · Cited by 1,211 · see citing works
Percutaneous Needle Biopsy of Skeletal Muscle in Physiological and Clinical Research
Bergström, J. P. (1975) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365517509095787 · Cited by 1,159 · see citing works
A Model-Based Method for Assessing Insulin Sensitivity From the Oral Glucose Tolerance Test
Mari, A., Pacini, G., Murphy, E., Ludvik, B. H., & Nolan, J. J. (2001) · Diabetes Care · doi:10.2337/diacare.24.3.539 · Cited by 805 · see citing works
Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men
Pennings, B., Boirie, Y., Senden, J. M., Gijsen, A. P., Kuipers, H., & van Loon, L. J. (2011) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.008102 · Cited by 656 · see citing works
Self-citation
What Is Sarcopenia?
Evans, W. J. (1995) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/50a.special_issue.5 · Cited by 606 · see citing works
Use of a heated superficial hand vein as an alternative site for the measurement of amino acid concentrations and for the study of glucose and alanine kinetics in man
Abumrad, N. N., Rabin, D. D., Diamond, M. P., & LACY, W. W. (1981) · Metabolism · doi:10.1016/0026-0495(81)90074-3 · Cited by 569 · see citing works
Aging is associated with diminished accretion of muscle proteins after the ingestion of a small bolus of essential amino acids
Katsanos, C. S., Kobayashi, H., Sheffield‐Moore, M., Aarsland, A. A., & Wolfe, R. R. (2005) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/82.5.1065 · Cited by 504 · see citing works
Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis
Wolfe, R. R., & Chinkes, D. L. (2004) · Medical Entomology and Zoology · Cited by 497 · see citing works
Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle
Greenhaff, P. L., Karagounis, L. G., Peirce, N., Simpson, E. J., Hazell, M., Layfield, R., Wackerhage, H., Smith, K., Atherton, P., Selby, A., et al. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90411.2008 · Cited by 495 · see citing works
The Response of Muscle Protein Anabolism to Combined Hyperaminoacidemia and Glucose-Induced Hyperinsulinemia Is Impaired in the Elderly<sup>1</sup>
Volpi, E., Mittendorfer, B., Rasmussen, B. B., & Wolfe, R. R. (2000) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.85.12.7021 · Cited by 464 · see citing works
Impaired anabolic response of muscle protein synthesis is associated with S6K1 dysregulation in elderly humans
Guillet, C., Prod'homme, M., Balage, M., Gachon, P., Giraudet, C., Morin, L., Grizard, J., & Boirie, Y. (2004) · The FASEB Journal · doi:10.1096/fj.03-1341fje · Cited by 412 · see citing works
The Rate of Protein Digestion affects Protein Gain Differently during Aging in Humans
Dangin, M., Guillet, C., Garcia‐Rodenas, C., Gachon, P., Bouteloup‐Demange, C., Reiffers‐Magnani, K., Fauquant, J., Ballèvre, O., & Beaufrère, B. (2003) · The Journal of Physiology · doi:10.1113/jphysiol.2002.036897 · Cited by 395 · see citing works
Ingestion of a protein hydrolysate is accompanied by an accelerated in vivo digestion and absorption rate when compared with its intact protein.
Koopman, R., Crombach, N., Gijsen, A., Walrand, S., Fauquant, J., Kies, A., Lemosquet, S., Saris, W., Boirie, Y., & van Loon, L. V. (2009) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.2009.27474 · Cited by 367 · see citing works
Self-citation
Blood flow and muscle metabolism: a focus on insulin action
Clark, M. G., Wallis, M. G., Barrett, E. J., Keske, M. A., Richards, S. M., Clerk, L. H., & Rattigan, S. (2003) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00408.2002 · Cited by 355 · see citing works
Amino acid absorption and subsequent muscle protein accretion following graded intakes of whey protein in elderly men
Pennings, B., Groen, B., de Lange, A., Gijsen, A. P., Zorenc, A. H., Senden, J. M. G., & van Loon, L. J. C. (2012) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00517.2011 · Cited by 326 · see citing works
Self-citation
The design and analysis of isotope experiments
Zilversmit, D. B. (1960) · The American Journal of Medicine · doi:10.1016/0002-9343(60)90117-0 · Cited by 321 · see citing works
Combined ingestion of protein and free leucine with carbohydrate increases postexercise muscle protein synthesis in vivo in male subjects
Koopman, R., Wagenmakers, A. J. M., MANDERS, R. J. F., Zorenc, A. H. G., Senden, J. M., Gorselink, M., KEIZER, H. A., & van Loon, L. J. C. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00413.2004 · Cited by 316 · see citing works
Exercising before protein intake allows for greater use of dietary protein–derived amino acids for de novo muscle protein synthesis in both young and elderly men
Pennings, B., Koopman, R., Beelen, M., Senden, J. M., Saris, W. H., & van Loon, L. J. (2011) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.2010.29649 · Cited by 311 · see citing works
Independent and Combined Effects of Amino Acids and Glucose after Resistance Exercise
MILLER, S. L., TIPTON, K. D., CHINKES, D. L., WOLF, S. E., & WOLFE, R. R. (2003) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000053910.63105.45 · Cited by 283 · see citing works
Effect of insulin on human skeletal muscle protein synthesis is modulated by insulin-induced changes in muscle blood flow and amino acid availability
Fujita, S., Rasmussen, B. B., Cadenas, J. G., Grady, J. J., & Volpi, E. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00271.2005 · Cited by 264 · see citing works
The Role of Milk- and Soy-Based Protein in Support of Muscle Protein Synthesis and Muscle Protein Accretion in Young and Elderly Persons
Phillips, S. M., Tang, J. E., & Moore, D. R. (2009) · Journal of the American College of Nutrition · doi:10.1080/07315724.2009.10718096 · Cited by 243 · see citing works
Insulin Stimulates Human Skeletal Muscle Protein Synthesis via an Indirect Mechanism Involving Endothelial-Dependent Vasodilation and Mammalian Target of Rapamycin Complex 1 Signaling
Timmerman, K. L., Lee, J. L., Dreyer, H. C., Dhanani, S., Glynn, E. L., Fry, C. S., Drummond, M. J., Sheffield‐Moore, M., Rasmussen, B. B., & Volpi, E. (2010) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2009-2696 · Cited by 180 · see citing works
Differences in Muscle Protein Synthesis and Anabolic Signaling in the Postabsorptive State and in Response to Food in 65–80 Year Old Men and Women
Smith, G. I., Atherton, P., Villareal, D. T., Frimel, T. N., Rankin, D., Rennie, M. J., & Mittendorfer, B. (2008) · PLoS ONE · doi:10.1371/journal.pone.0001875 · Cited by 168 · see citing works
Quantitative UPLC-MS/MS analysis of underivatised amino acids in body fluids is a reliable tool for the diagnosis and follow-up of patients with inborn errors of metabolism
Waterval, H. W. A. H., Scheijen, J. L. J. M., Ortmans-Ploemen, M. M. J. C., der Poel, C. D. H.-V., & Bierau, J. (2009) · Clinica Chimica Acta · doi:10.1016/j.cca.2009.06.023 · Cited by 164 · see citing works
Supraphysiological hyperinsulinaemia is necessary to stimulate skeletal muscle protein anabolism in older adults: evidence of a true age-related insulin resistance of muscle protein metabolism
Fujita, S., Glynn, E. L., Timmerman, K. L., Rasmussen, B. B., & Volpi, E. (2009) · Diabetologia · doi:10.1007/s00125-009-1430-8 · Cited by 164 · see citing works
Leucine and phenylalanine kinetics during mixed meal ingestion: a multiple tracer approach
Biolo, G., Tessari, P., Inchiostro, S., Bruttomesso, D., Fongher, C., Sabadin, L., Fratton, M. G., Valerio, A., & Tiengo, A. (1992) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1992.262.4.e455 · Cited by 155 · see citing works
Coingestion of carbohydrate with protein does not further augment postexercise muscle protein synthesis
Koopman, R., Beelen, M., Stellingwerff, T., Pennings, B., Saris, W. H. M., Kies, A. K., Kuipers, H., & van Loon, L. J. C. (2007) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00135.2007 · Cited by 150 · see citing works
Pharmacological Vasodilation Improves Insulin-Stimulated Muscle Protein Anabolism but Not Glucose Utilization in Older Adults
Timmerman, K. L., Lee, J. L., Fujita, S., Dhanani, S., Dreyer, H. C., Fry, C. S., Drummond, M. J., Sheffield-Moore, M., Rasmussen, B. B., & Volpi, E. (2010) · Diabetes · doi:10.2337/db10-0415 · Cited by 149 · see citing works
Combined ingestion of protein and carbohydrate improves protein balance during ultra-endurance exercise
Koopman, R., Pannemans, D. L. E., Jeukendrup, A. E., Gijsen, A. P., Senden, J. M. G., Halliday, D., Saris, W. H. M., van Loon, L. J. C., & Wagenmakers, A. J. M. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00543.2003 · Cited by 147 · see citing works
Self-citation
Differential Regulation of Protein Dynamics in Splanchnic and Skeletal Muscle Beds by Insulin and Amino Acids in Healthy Human Subjects
Nygren, J., & Nair, K. S. (2003) · Diabetes · doi:10.2337/diabetes.52.6.1377 · Cited by 147 · see citing works
Differential regulation of amino acid exchange and protein dynamics across splanchnic and skeletal muscle beds by insulin in healthy human subjects.
Meek, S. E. M., Persson, M., Ford, G. C., & Nair, K. S. (1998) · Diabetes · doi:10.2337/diabetes.47.12.1824 · Cited by 122 · see citing works
Insulin dose-dependent reductions in plasma amino acids in man
Fukagawa, N. K., Minaker, K. L., Young, V. R., & Rowe, J. W. (1986) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1986.250.1.e13 · Cited by 120 · see citing works
Amino acid metabolism and regulatory effects in aging
Timmerman, K., & Volpi, E. (2008) · Current opinion in clinical nutrition and metabolic care · doi:10.1097/mco.0b013e3282f2a592 · Cited by 104 · see citing works
Age‐related anabolic resistance after endurance‐type exercise in healthy humans
Durham, W. J., Casperson, S. L., Dillon, E. L., Keske, M. A., Paddon‐Jones, D., Sanford, A. P., Hickner, R. C., Grady, J. J., & Sheffield‐Moore, M. (2010) · The FASEB Journal · doi:10.1096/fj.09-150177 · Cited by 88 · see citing works
The production of intrinsically labeled milk protein provides a functional tool for human nutrition research
van Loon, L. J. C., Boirie‌, Y., Gijsen, A. P., Fauquant, J., de Roos, A. L., Kies, A. K., Lemosquet, S., Saris, W. H. M., & Koopman, R. (2009) · Journal of Dairy Science · doi:10.3168/jds.2009-2317 · Cited by 85 · see citing works
Self-citation
Amino acids are necessary for the insulin-induced activation of mTOR/S6K1 signaling and protein synthesis in healthy and insulin resistant human skeletal muscle
Drummond, M. J., Bell, J. A., Fujita, S., Dreyer, H. C., Glynn, E. L., Volpi, E., & Rasmussen, B. B. (2008) · Clinical Nutrition · doi:10.1016/j.clnu.2008.01.012 · Cited by 80 · see citing works
Insulin-mediated increase in blood flow is impaired in the elderly
Meneilly, G. S., Elliot, T. K., Bryer‐Ash, M., & Floras, J. S. (1995) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.80.6.7775638 · Cited by 68 · see citing works
The production of intrinsically labeled milk and meat protein is feasible and provides functional tools for human nutrition research
Pennings, B., Pellikaan, W. F., Senden, J. M., van Vuuren, A. M., Sikkema, J., & van Loon, L. J. C. (2011) · Journal of Dairy Science · doi:10.3168/jds.2011-4451 · Cited by 53 · see citing works
The curious case of anabolic resistance: old wives' tales or new fables?
Burd, N. A., Wall, B. T., & van Loon, L. J. C. (2011) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01343.2011 · Cited by 52 · see citing works
Self-citation
Insulin and muscle protein turnover in humans: stimulatory, permissive, inhibitory, or all of the above?
Phillips, S. M. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90569.2008 · Cited by 37 · see citing works
High-dose insulin administration is associated with hypoaminoacidemia during cardiac surgery
Hatzakorzian, R., Carvalho, G., Bui, H., Sato, T., Wykes, L., Tim, D. S., & Schricker, T. (2011) · Metabolism · doi:10.1016/j.metabol.2011.03.007 · Cited by 10 · see citing works
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Alberti (1998) · doi:10.1002/(sici)1096-9136(199807)15:7

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Gorissen, S., Burd, N., Hamer, H., Gijsen, A., Groen, B. B. L., & van Loon, L. V. (2014) · Journal of Clinical Endocrinology and Metabolism · doi:10.1210/jc.2013-3970 · Cited by 135 · see citing works
Self-citation
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Nongonierma, A. B., O’Keeffe, M. B., & Fitzgerald, R. J. (2015) · Springer eBooks · doi:10.1007/978-1-4939-2800-2_15 · Cited by 138 · see citing works
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Witard, O., Wardle, S., Macnaughton, L., Hodgson, A., & Tipton, K. (2016) · Nutrients · doi:10.3390/nu8040181 · Cited by 132 · see citing works
Strategies to maintain skeletal muscle mass in the injured athlete: Nutritional considerations and exercise mimetics
Wall, B. T., Morton, J. P., & van Loon, L. J. C. (2014) · European Journal of Sport Science · doi:10.1080/17461391.2014.936326 · Cited by 122 · see citing works
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Lamon, S., Morabito, A., Arentson‐Lantz, E., Knowles, O., Vincent, G. E., Condo, D., Alexander, S. E., Garnham, A., Paddon‐Jones, D., & Aisbett, B. (2021) · Physiological Reports · doi:10.14814/phy2.14660 · Cited by 110 · see citing works
Mycoprotein ingestion stimulates protein synthesis rates to a greater extent than milk protein in rested and exercised skeletal muscle of healthy young men: a randomized controlled trial
Monteyne, A. J., Coelho, M. O., Porter, C., Abdelrahman, D. R., Jameson, T. S., Jackman, S. R., Blackwell, J. R., Finnigan, T. J., Stephens, F. B., Dirks, M. L., et al. (2020) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/nqaa092 · Cited by 110 · see citing works
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Luiking, Y. C., Deutz, N. E. P., Memelink, R. G., Verlaan, S., & Wolfe, R. R. (2014) · Nutrition Journal · doi:10.1186/1475-2891-13-9 · Cited by 101 · see citing works
Growing older with health and vitality: a nexus of physical activity, exercise and nutrition
Witard, O. C., McGlory, C., Hamilton, D. L., & Phillips, S. M. (2016) · Biogerontology · doi:10.1007/s10522-016-9637-9 · Cited by 91 · see citing works
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Carbohydrate coingestion delays dietary protein digestion and absorption but does not modulate postprandial muscle protein accretion.
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0061 NEUROPSYCHOLOGIAL FUNCTIONING OF FIRST-EPISODE PSYCHOSIS PATIENTS
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Authors: Hamer, H. M., Wall, B. T., Kiskini, A., de Lange, A., Groen, B. B. L., Bakker, J. A., Gijsen, A. P., Verdijk, L. B., & van Loon, L. J. C.
Affiliations: Maastricht University Medical Centre (NL)
ORCID: Hamer, Henrike M
Wall, Benjamin T
Groen, Bart B L
Bakker, Jaap A
Verdijk, Lex B
Venue: Nutrition & Metabolism
Publisher: Springer Science and Business Media LLC
Published: 2013-01-25
Volume: 10
Issue: 1
Pages: 15-15
ISSN: 1743-7075 (ISSN-L)
DOI: 10.1186/1743-7075-10-15
PMC: PMC3585863
OpenAlex: W2112603567
Cited by: 44
References: 45
Citations by year: 2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 8 · 2015: 7 · 2014: 7 · 2013: 3
FWCI: 4.83
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Nutrition & Metabolism (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Research Publications (Maastricht University) (publishedVersion, cc-by)
Research Bank (Australian Catholic University) (submittedVersion, cc-by)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Research Publications (Maastricht University) (submittedVersion, other-oa)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords: muscle protein accretion, older men, insulin response, phenylalanine incorporation, muscle protein synthesis, sarcopenia, dietary protein

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Carbohydrate co-ingestion with protein does not further augment post-prandial muscle protein accretion in older men

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