Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum
Open accessAbstract
BACKGROUND: Microalgae have been an emerging biofuel resource; however, the germplasm improvement has been slow due to the lack of molecular tools. Pyruvate dehydrogenase kinase (PDK) deactivates the pyruvate dehydrogenase complex (PDC) which catalyzes the oxidative decarboxylation of pyruvate. Acetyl-CoA production via PDC is important in plant tissues that are active in fatty acid synthesis. RESULTS: A 1261-bp cDNA of a putative PDK gene (PtPDK) was cloned from a diatom Phaeodactylum tricornutum, and PtPDK antisense knockdown transgenic diatoms were generated. Both PtPDK transcript abundance and enzyme activity were reduced significantly due to antisense knockdown of PtPDK. Neutral lipid content of transgenic diatom cells increased up to 82% as determined by Nile red staining, and fatty acid composition was not altered. Transgenic cells showed slightly lower growth rate but similar cell size with the wild type, hence retaining similar biomass productivity. CONCLUSIONS: This work first obtained a successful engineered diatom regulating a key gene involved in lipid metabolism. Our findings also provide powerful indications in enhancing microalgal lipid production by metabolic engineering for biofuel industry.
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Exceptionally high content of total lipids was noted in Prymnesium parvum (65.79%), Vischeria vischeri (65.16%), Nannochloropsis granulata CCMP525 (60.35%), Chlorella vulgaris (67.1%), and Tetradesmus deserticola JNU19 (62.4%).
“The exceptionally high content of total lipids was noted in microalgae Prymnesium parvum (65.79%) [99], Vischeria vischeri (65.16%) [100], Nannochloropsis granulata CCMP525 (60.35%) [101], Chlorella vulgaris (67.1%) [99], Tetradesmus deserticola JNU19 (62.4%) [102].”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [101]
The Materials and methods and Results sections study Phaeodactylum tricornutum and report its neutral lipid content, not total lipid content for the five species in the citing sentence. Searches for the named species and their percentages found no passage in our copy addressing those figures. No page numbers are available in the indexed copy. (LLM, conf=0.78)
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The generational shift in biofuels: A path toward sustainable energy solutions
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Metabolic Engineering of Microalgae for Biofuel Production
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“Each sample was tested in triplicate, and expression levels were referenced against the endogenous gene β -actin and TBP (TATA box binding protein), which were selected based on their stable expression across different conditions in P. tricornutum , as veri fi ed in previous studies 105,106 .”
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“…the TAG increase following 48 h of TBP treatment correlates to an upregulation of glycolysisrelated genes and fatty acid biosynthesis, which is compelling considering that glycolysis may result in accumulated intracellular acetyl coenzymeA, the material of fatty acid synthesis (Ma et al. 2014).”
BackgroundMethodMicroalgae Applications to Bone Repairing Processes: A Review
Payares, L. M. A., Púa, L. D. C. G., Pareja, L. A. D. M., Méndez, S. C. P., & Paredes, V. (2023) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.2c01389 · Cited by 19 · see citing works
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“In another intriguing experiment, antisense cDNA constructs from the microalgae Phaeodactylum tricornutum were used to generate transgenic diatoms that exhibited putative pyruvate dehydrogenase kinase (PtPDK) knockdown characteristics (Ma et al. 2014).”
Relationship between acyl-lipid and sterol metabolisms in diatoms
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“of mitochondrial acetyl-CoA pool in diatoms [35].”
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“Especially, stimulating fatty acid-elongase, D5-desaturase, Acyl-ACP, and Acetyl-CoA carboxylase were demonstrated to increase the lipid and fatty acids contents [69,70], while transcripts associated with multi-functional lipase/acyltransferase, pyruvate metabolism were silenced to the increased yield of lipid content…”
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Rathod, J. P., Gade, R. M., Rathod, D. R., & Dudhare, M. S. (2017) · International Journal of Current Microbiology and Applied Sciences · doi:10.20546/ijcmas.2017.612.373 · Cited by 18 · see citing works
“…2012 Δ4 desaturase Increased accumulation of FA 16:4 Zäuner et al., 2012 P. tricornutum Pyruvate dehydroganase kinase Neutral lipids increase by 82% Ma et al., 2014 Malic enzyme Total lipids increased by 2.5 times Xue et al., 2014 Δ5 desaturase 65% more neutral FA with 58% increased…”
BackgroundInfluentialNew strategies enhancing feasibility of microalgal cultivations
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Heterotrophic modification of Phaeodactylum tricornutum Bohlin
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Aromatic Amino Acids: Exploring Microalgae as a Potential Biofactory
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“[52] 35% increase in neutral lipid accumulation, 76% increase in the valuable omega-3, eicosapentaenoic acid (EPA) Overexpression of the endogenous diacylglycerol acyltransferase 2, transformed via electroporation.”
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“As the end product of glycolysis, pyruvate links glycolysis with TCA cycle and lipid biosynthesis, thus pyruvate is also considered a regulative metabolite for adjusting lipid biosynthesis (Goodson et al. 2011; Ma et al. 2014; Avidan and Pick 2015).”
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“, 2018), while PYK is involved in the lipid biosynthesis of diatoms (Ma et al., 2014).”
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“Knockdown of the latter induces accumulation of NL in the cell (Ma et al ., 2014).”
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“Nannochloropsis salina was selected in this study because of its high lipid content and interesting fatty acid composition (Bellou and Aggelis 2012; Ma et al. 2014b).”
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A comparison of lipid content metrics using six species from the genus Halamphora (Bacillariophyta)
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Self-citation
- Authors
- Ma, Y.-H., Wang, X., Niu, Y.-F., Yang, Z.-K., Zhang, M.-H., Wang, Z.-M., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
- Affiliations
- Jinan University (CN)
- Venue
- Microbial Cell Factories
- Published
- 2014-08-09
- Volume
- 13
- Issue
- 1
- Pages
- 100-100
- ISSN
- 1475-2859 (ISSN-L)
- PMC
- PMC4251698
- OpenAlex
- W2108487405
- Cited by
- 106
- References
- 30
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 2 · 2023: 10 · 2022: 8 · 2021: 12 · 2020: 9 · 2019: 19 · 2018: 10 · 2017: 11 · 2016: 9 · 2015: 4
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Microbial Cell Factories (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repository of Guangzhou Institute of Energy Research, Chinese Academy of Sciences (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- pyruvate dehydrogenase kinase, Phaeodactylum tricornutum, diatoms, neutral lipid accumulation, pyruvate dehydrogenase complex, antisense knockdown, microalgal lipid production, metabolic engineering, biofuels, fatty acid composition, acetyl-CoA
- MeSH terms
- Pyruvate Dehydrogenase Acetyl-Transferring Kinase, RNA, Antisense, Protein Serine-Threonine Kinases, Diatoms, Lipid Metabolism, Gene Knockdown Techniques, Microalgae, Metabolic Engineering
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