Production of medium-chain fatty acids and higher alcohols by a synthetic co-culture grown on carbon monoxide or syngas
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BACKGROUND: Synthesis gas, a mixture of CO, H2, and CO2, is a promising renewable feedstock for bio-based production of organic chemicals. Production of medium-chain fatty acids can be performed via chain elongation, utilizing acetate and ethanol as main substrates. Acetate and ethanol are main products of syngas fermentation by acetogens. Therefore, syngas can be indirectly used as a substrate for the chain elongation process. RESULTS: Here, we report the establishment of a synthetic co-culture consisting of Clostridium autoethanogenum and Clostridium kluyveri. Together, these bacteria are capable of converting CO and syngas to a mixture of C4 and C6 fatty acids and their respective alcohols. The co-culture is able to grow using solely CO or syngas as a substrate, and presence of acetate significantly stimulated production rates. The co-culture produced butyrate and caproate at a rate of 8.5 ± 1.1 and 2.5 ± 0.63 mmol/l/day, respectively. Butanol and hexanol were produced at a rate of 3.5 ± 0.69 and 2.0 ± 0.46 mmol/l/day, respectively. The pH was found to be a major factor during cultivation, influencing the growth performance of the separate strains and caproate toxicity. CONCLUSION: This co-culture poses an alternative way to produce medium-chain fatty acids and higher alcohols from carbon monoxide or syngas and the process can be regarded as an integration of syngas fermentation and chain elongation in one growth vessel.
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Diender et al. demonstrated production of butyrate, caproate, butanol and hexanol by co-culture of C. kluyveri and C. autoethanogenum fed with CO and H2.
“Diender et al. recently demonstrated production of butyrate, caproate, butanol and hexanol by co-culture of C. kluyveri and C. autoethanogenum fed with CO and H2 (Diender et al., 2016).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Diender et al., 2016
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- Authors
- Diender, M., Stams, A. J. M., & Sousa, D. Z.
- Affiliations
- Wageningen University & Research (NL)
- Corresponding author
- Diender, Martijn
- Venue
- Biotechnology for Biofuels
- Published
- 2016-04-02
- Volume
- 9
- Issue
- 1
- Pages
- 82-82
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC4818930
- OpenAlex
- W2316195511
- Cited by
- 259
- References
- 35
- Citations by year
- 2026: 20 · 2025: 28 · 2024: 35 · 2023: 27 · 2022: 32 · 2021: 29 · 2020: 25 · 2019: 22 · 2018: 17 · 2017: 19 · 2016: 5
- FWCI
- 23.02
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion, cc-by)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
- Funders
- Ministerie van Onderwijs, Cultuur en Wetenschap (SIAM 024.002.002)
- European Research Council (323009)
- Topics
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- medium-chain fatty acids, higher alcohols, syngas fermentation, carbon monoxide, chain extension, Clostridium autoethanogenum, Clostridium kluyveri, butyrate, caproate, butanol, hexanol, acetate, pH effect, acetogenic bacteria
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