Exosomes/tricalcium phosphate combination scaffolds can enhance bone regeneration by activating the PI3K/Akt signaling pathway
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BACKGROUND: Recently, accumulating evidence has shown that exosomes, the naturally secreted nanocarriers of cells, can exert therapeutic effects in various disease models in the absence of parent cells. However, application of exosomes in bone defect repair and regeneration has been rarely reported, and little is known regarding their underlying mechanisms. METHODS: Exosomes derived from human-induced pluripotent stem cell-derived mesenchymal stem cells (hiPS-MSC-Exos) were combined with tricalcium phosphate (β-TCP) to repair critical-sized calvarial bone defects, and the efficacy was assessed by histological examination. We evaluated the in vitro effects of hiPSC-MSC-Exos on the proliferation, migration, and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) by cell-counting, scratch assays, and qRT-PCR, respectively. Gene expression profiling and bioinformatics analyses were also used to identify the underlying mechanisms in the repair. RESULTS: We found that the exosome/β-TCP combination scaffolds could enhance osteogenesis as compared to pure β-TCP scaffolds. In vitro assays showed that the exosomes could release from β-TCP and could be internalized by hBMSCs. In addition, the internalization of exosomes into hBMSCs could profoundly enhance the proliferation, migration, and osteogenic differentiation of hBMSCs. Furthermore, gene expression profiling and bioinformatics analyses demonstrated that exosome/β-TCP combination scaffolds significantly altered the expression of a network of genes involved in the PI3K/Akt signaling pathway. Functional studies further confirmed that the PI3K/Akt signaling pathway was the critical mediator during the exosome-induced osteogenic responses of hBMSCs. CONCLUSIONS: We propose that the exosomes can enhance the osteoinductivity of β-TCP through activating the PI3K/Akt signaling pathway of hBMSCs, which means that the exosome/β-TCP combination scaffolds possess better osteogenesis activity than pure β-TCP scaffolds. These results indicate that naturally secreted nanocarriers-exosomes can be used as a bioactive material to improve the bioactivity of the biomaterials, and that hiPS-MSC-Exos combined with β-TCP scaffolds can be potentially used for repairing bone defects.
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MSC-exosome/β-TCP composite scaffolds had better healing functions on rat calvarial defects.
“In addition, Zhang et al. found MSC-exosome/β-TCP composite scaffolds had better healing functions on rat calvarial defects [88].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [88]
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“9,15,44 We have prepared a novel scaffold (PAA/triCaPNP) by the cross-linking method as described.”
BackgroundMethod
- Authors
- Zhang, J., Liu, X., Li, H., Chen, C., Hu, B., Niu, X., Li, Q., Zhao, B., Xie, Z., & Wang, Y.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Shanghai Sixth People's Hospital (CN)
- Corresponding author
- Xie, Zongping
- Wang, Yang
- Venue
- Stem Cell Research & Therapy
- Published
- 2016-09-20
- Volume
- 7
- Issue
- 1
- Pages
- 136-136
- ISSN
- 1757-6512 (ISSN-L)
- PMC
- PMC5028974
- OpenAlex
- W2523417713
- Cited by
- 431
- References
- 46
- Citations by year
- 2026: 25 · 2025: 44 · 2024: 54 · 2023: 47 · 2022: 54 · 2021: 69 · 2020: 54 · 2019: 41 · 2018: 31 · 2017: 12
- FWCI
- 13.48
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Stem Cell Research & Therapy (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81472152, 81371938, 31470918, 81572120)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
- Keywords
- exosomes, tricalcium phosphate, bone regeneration, PI3K/Akt signaling, induced pluripotent stem cell-derived mesenchymal stem cells, bone marrow-derived mesenchymal stem cells, osteogenic differentiation, calvarial defect, exosome internalization
- MeSH terms
- Animals, Bone Regeneration, Calcium Phosphates, Cell Differentiation, Cell Movement, Cells, Cultured, Gene Expression Regulation, Humans, Osteogenesis, Skull, Signal Transduction, Rats, Sprague-Dawley, Phosphatidylinositol 3-Kinases, Gene Expression Profiling, Cell Proliferation, Proto-Oncogene Proteins c-akt, Rats, Tissue Scaffolds, Exosomes, Induced Pluripotent Stem Cells, Mesenchymal Stem Cells
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