Cited by 39Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsSkin Protection and Aging

Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence

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Marthandan, Shiva, Menzel, Uwe, Priebe, Steffen, Groth, Marco, Guthke, Reinhard, Platzer, Matthias, Hemmerich, Peter, Kaether, Christoph +1 more·2016·Biological Research

Abstract

BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, "Cell cycle" was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, "DNA repair" and "replication" pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with "DNA repair" and "replication" less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.

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Authors
Marthandan, S., Menzel, U., Priebe, S., Groth, M., Guthke, R., Platzer, M., Hemmerich, P., Kaether, C., & Diekmann, S.
Affiliations
  • Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) (DE)
  • Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI) (DE)
Corresponding author
Marthandan, Shiva
Venue
Biological Research
Published
2016-07-28
Volume
49
Issue
1
Pages
34-34
ISSN
  • 0716-9760 (ISSN-L)
  • 0717-6287
OpenAlex
W2491783232
Cited by
39
References
210
Citations by year
2026: 1 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 7 · 2021: 5 · 2020: 2 · 2019: 6 · 2018: 5 · 2017: 2
FWCI
3.49
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Biological Research (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Informationszentrum Lebenswissenschaften (ZB MED) (submittedVersion, cc-by)
Funders
Bundesministerium für Bildung und Forschung– BMBF (0315581)
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
Keywords
cellular senescence, replicative senescence, radiation-induced senescence, gamma irradiation, primary human fibroblasts, DNA repair, DNA replication, cell cycle regulation, transcriptome, differential gene expression, DNA damage accumulation, MRC-5 cells
MeSH terms
Analysis of Variance, beta-Galactosidase, Cells, Cultured, DNA Damage, DNA Repair, DNA Replication, Fibroblasts, Gamma Rays, Humans, Lung, Male, Time Factors, Immunoblotting, Down-Regulation, Up-Regulation, Cellular Senescence, Sequence Analysis, RNA, Gene Expression Profiling, Aborted Fetus

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