Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes

Keung, Wendy; Boheler, Kenneth R; Li, Ronald A

doi:10.1186/scrt406

Cited by 88Pluripotent Stem Cells ResearchCardiac electrophysiology and arrhythmiasTissue Engineering and Regenerative Medicine

Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes

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Keung, Wendy, Boheler, Kenneth R, Li, Ronald A·2014·Stem Cell Research & Therapy

DOI 10.1186/scrt406PMC PMC4055054

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Abstract

Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells, are abundant sources of cardiomyocytes (CMs) for cell replacement therapy and other applications such as disease modeling, drug discovery and cardiotoxicity screening. However, hPSC-derived CMs display immature structural, electrophysiological, calcium-handling and metabolic properties. Here, we review various biological as well as physical and topographical cues that are known to associate with the development of native CMs in vivo to gain insights into the development of strategies for facilitated maturation of hPSC-CMs.

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Major hallmarks regarded as a general consensus of cardiomyocyte maturation include myofibril maturation, electrophysiology, calcium handling, metabolic maturation, hypertrophy, and integration of physiology.
“a number of major hallmarks have been regarded as a general consensus of the field, including myofibril maturation, electrophysiology, calcium handling, metabolic maturation, hypertrophy, and integration of physiology.10,37,38”
From Single-cell RNA sequencing reveals maturation trajectory in human pluripotent stem cell-derived cardiomyocytes in engineered tissues (2023) · cites this paper as [37]
The sections on facilitated electrophysiological and calcium-handling maturation and facilitated metabolic maturation discuss those properties, while the Adrenergic system section links hypertrophy to cardiac growth and maturation. The paper also describes myofilament organization and physical function, but our copy does not present this six-item list as a field-wide consensus or identify “integration of physiology” as a distinct hallmark. The indexed passages have no page numbers. (LLM, conf=0.82)
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Rana P Anson B Engle S Will Y Characterization of human-induced pluripotent stem cell-derived cardiomyocytes: bioenergetics and utilization in safety screening Toxicol Sci 2012 130 117 131 10.1093/toxsci/kfs233 22843568 · DOI

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Authors: Keung, W., Boheler, K. R., & Li, R. A.
Affiliations: University of Hong Kong (HK)
Johns Hopkins University (US)
Corresponding author: Keung, Wendy
Li, Ronald A
ORCID: Keung, Wendy
Boheler, Kenneth R
Li, Ronald A
Venue: Stem Cell Research & Therapy
Publisher: Springer Science and Business Media LLC
Published: 2014-01-28
Volume: 5
Issue: 1
Pages: 17-17
ISSN: 1757-6512 (ISSN-L)
DOI: 10.1186/scrt406
PMC: PMC4055054
OpenAlex: W2122060214
Cited by: 87
References: 102
Citations by year: 2026: 2 · 2025: 1 · 2024: 10 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 13 · 2019: 13 · 2018: 10 · 2017: 10 · 2016: 8 · 2015: 8 · 2014: 3
FWCI: 0.91
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Stem Cell Research & Therapy (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
The HKU Scholars Hub (University of Hong Kong) (submittedVersion, cc-by-nc-nd)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords: human pluripotent stem cell-derived cardiomyocytes, cardiomyocyte maturation, electrophysiological maturation, calcium handling, cardiomyocyte metabolism, topographical cues, physical cues, embryonic stem cells, induced pluripotent stem cells, cardiomyocyte development
MeSH terms: Action Potentials, Cell Differentiation, Energy Metabolism, Humans, Paracrine Communication, Calcium Signaling, Myocytes, Cardiac, Pluripotent Stem Cells, Chromatin Assembly and Disassembly

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Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes

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