The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats
Abstract
6-Hydroxydopamine (6-OHDA) is one of the most widely used rat models for Parkinson's disease. There is ample evidence in the literature that 6-OHDA elicits its toxic manifestations through oxidant stress. In the present study, we evaluated the anti-parkinsonian effects of Withania somnifera extract, which has been reported to have potent anti-oxidant, anti-peroxidative and free radical quenching properties in various diseased conditions. Rats were pretreated with 100, 200 and 300 mg/kg b.w. of the W. somnifera extract orally for 3 weeks. On day 21, 2 microL of 6-OHDA (10 microg in 0.1% in ascorbic acid-saline) was infused into the right striatum while sham operated group received 2 microL of the vehicle. Three weeks after 6-OHDA injections, rats were tested for neurobehavioral activity and were killed 5 weeks after lesioning for the estimation of lipidperoxidation, reduced glutathione content, activities of glutathione-S-transferase, glutathione reductase, glutathione peroxidase, superoxide dismutase and catalase, catecholamine content, dopaminergic D2 receptor binding and tyrosine hydroxylase expression. W. somnifera extract was found to reverse all the parameters significantly in a dose-dependent manner. Thus, the study demonstrates that the extract of W. somnifera may be helpful in protecting the neuronal injury in Parkinson's disease.
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“Ahmad et al. [9] reported dose-dependent reversal of the 6-OHDA induced neurotoxicity mainly due to the strong antioxidant properties of WS extract.”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [9]
In the citing paper
“| WS extract | Dose-dependent reversal of 6-OHDA | Rats | Increase in strong antioxidant property, | [9] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [9]
In the citing paper
“| WS extract | Dose-dependent reversal of 6-OHDA | Rats | Increase in strong antioxidant property, | [9] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [9]
In the citing paper
“| WS extract | Dose-dependent reversal of 6-OHDA | Rats | Increase in strong antioxidant property, | [9] |”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [9]
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 316,394
The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585
Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42083-8 · Cited by 18,509
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels
Beauchamp, C., & Fridovich, I. (1971) · Analytical Biochemistry · doi:10.1016/0003-2697(71)90370-8 · Cited by 12,892
Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1620 · Cited by 7,029
Indian Journal of Experimental Biology
(2022) · Indian Journal of Experimental Biology · doi:10.56042/ijeb · Cited by 3,397
Jollow, D. J., Mitchell, J. R., ZAMPAGLIONE, N. G., & Gillette, J. R. (2008) · Pharmacology · doi:10.1159/000136485 · Cited by 3,291
Biology of disease: free radicals and tissue injury.
Freeman, B. Α., & Jd, C. (1982) · PubMed · Cited by 3,038
Handbook of methods for oxygen radical research
Greenwald, R. A. (1987) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(87)80012-6 · Cited by 2,388
Purification and characterization of the flavoenzyme glutathione reductase from rat liver.
Carlberg, I., & Mannervik, B. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41206-4 · Cited by 2,024
Glutathione metabolism and its selective modification.
Meister, A. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)77815-6 · Cited by 2,012
CRC handbook of methods for oxygen radical research
Bogomolets, B. B. (1985) · Experimental Gerontology · doi:10.1016/0531-5565(85)90021-x · Cited by 1,551
Fridovich, I. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83102-7 · Cited by 970
Oxygen radicals and the nervous system
Halliwell, B. B., & Gutteridge, J. M. C. (1985) · Trends in Neurosciences · doi:10.1016/0166-2236(85)90010-4 · Cited by 823
Oxidative processes and antioxidative defense mechanisms in the aging brain 1
Reiter, R. J. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.7.7737461 · Cited by 801
Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review.
Mishra, L. C., Singh, B. B., & Dagenais, S. (2000) · PubMed · Cited by 776
Differential distribution of glutathione and glutathione-related enzymes in rabbit kidney
Mohandas, J., Marshall, J. J., Duggin, G. G., HORVATH, J. S., & TILLER, D. J. (1984) · Biochemical Pharmacology · doi:10.1016/0006-2952(84)90353-8 · Cited by 738
THE JOURNAL OF BIOLOGICAL CHEMISTRY.
Hanukoglu, I., & Jefcoate, C. R. (1905) · JAMA · doi:10.1001/jama.1905.02510220045008 · Cited by 612
Low activities of glutathione-related enzymes as factors in the genesis of urinary bladder cancer.
Mohandas, J., Marshall, J. J., Duggin, G. G., HORVATH, J. S., & TILLER, D. J. (1984) · PubMed · Cited by 543
Przedbroski, S., Leviver, M., Jiang, H., Ferreira, M., Jackson-Lewis, V. R., Donaldson, D. M., & Togasaki, D. M. (1995) · Neuroscience · doi:10.1016/0306-4522(95)00066-r · Cited by 453
Effect of sulfhydryl reagents on peroxidation in microsomes
Utley, H. G., Bernheim, F., & Hochstein, P. E. (1967) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(67)90273-1 · Cited by 446
Hudson, J. L., van Horne, C. G., Strömberg, I., Brock, S., Clayton, J., Masserano, J. M., Hoffer, B. J., & Gerhardt, G. A. (1993) · Brain Research · doi:10.1016/0006-8993(93)90576-9 · Cited by 377
Mechanisms of action of 6-hydroxydopamine
Sachs, C., & JONSSON, G. (1975) · Biochemical Pharmacology · doi:10.1016/0006-2952(75)90304-4 · Cited by 358
Schwarting, R. K. W., & Huston, J. P. (1996) · Progress in Neurobiology · doi:10.1016/s0301-0082(96)00015-9 · Cited by 356
Baez, S., Segura‐Aguilar, J., Widersten, M., Johansson, A.-S., & Mannervik, B. (1997) · Biochemical Journal · doi:10.1042/bj3240025 · Cited by 340
Bhattacharya, A., Ghosal, S., & Bhattacharya, S. K. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(00)00309-3 · Cited by 288
Matuszak, Z., Reszka, K. J., & Chignell, C. F. (1997) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(96)00614-4 · Cited by 288
Methamphetamine-induced neuronal damage: A possible role for free radicals
Vito, M. J. D., & Wagner, G. C. (1989) · Neuropharmacology · doi:10.1016/0028-3908(89)90130-5 · Cited by 270
Intracellular Protein Catabolism
Suzuki, K., & Bond, J. S. (1996) · Advances in experimental medicine and biology · doi:10.1007/978-1-4613-0335-0 · Cited by 248
A 6-hydroxydopamine-induced selective parkinsonian rat model
Perese, D. A., Ulman, J. N., Viola, J. J., Ewing, S. E., & Bankiewicz, K. S. (1989) · Brain Research · doi:10.1016/0006-8993(89)90597-0 · Cited by 241
Partial lesion of the substantia nigra: relation between extent of lesion and rotational behavior
Carman, L. S., Gage, F. H., & Shults, C. W. (1991) · Brain Research · doi:10.1016/0006-8993(91)90835-j · Cited by 231
Oxy-radical toxicity in catecholamine neurons.
Cohen, G. (1984) · PubMed · Cited by 224
Free Radical‐Generated Neurotoxicity of 6‐Hydroxydopamine
Kumar, R., Agarwal, A. K., & Seth, P. K. (1995) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1995.64041703.x · Cited by 203
Cadet, J. L., Katz, M., Jackson-Lewis, V. R., & Fahn, S. (1989) · Brain Research · doi:10.1016/0006-8993(89)91530-8 · Cited by 163
Zafar, K. S., Siddiqui, A., Sayeed, I., Ahmad, M., Salim, S., & Islam, F. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01531.x · Cited by 159
Self-citationBetz, H., Far, O., Airas, J. M., Connor, V., Bofill-Cardona, E., Nanoff, C., Freissmuth, M., Boehm, S., & Karschin, A. (2010) · Journal of Neurochemistry · doi:10.1111/(issn)1471-4159 · Cited by 158
Effect of ashwagandha on lipid peroxidation in stress-induced animals
Dhuley, J. N. (1998) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(97)00151-7 · Cited by 157
A comparative pharmacological investigation of Ashwagandha and Ginseng
Grandhi, A., Mujumdar, A. M., & Patwardhan, B. (1994) · Journal of Ethnopharmacology · doi:10.1016/0378-8741(94)01119-2 · Cited by 142
Perumal, A. S., Gopal, V. B., Tordzro, W. K., Cooper, T. B., & Cadet, J. L. (1992) · Brain Research Bulletin · doi:10.1016/0361-9230(92)90142-k · Cited by 120
Intracellular protein catabolism
Bohley, P., & Riemann, S. (1977) · De Gruyter eBooks · doi:10.1515/9783112650080-039 · Cited by 115
Hu, X., Wachtel, S. R., Galloway, M., & White, F. J. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-07-02318.1990 · Cited by 112
Effect of Withania somnifera on DMBA induced carcinogenesis
Davis, L., & Kuttan, G. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(00)00404-9 · Cited by 100
Wu, W.-R., & Xing-zu, Z. (1999) · Life Sciences · doi:10.1016/s0024-3205(99)00232-5 · Cited by 81
Evidence for free radical scavenging activity of Ashwagandha root powder in mice.
Panda, S., & Kar, A. (1997) · PubMed · Cited by 71
Huang, J., & Philbert, M. A. (1996) · Brain Research · doi:10.1016/0006-8993(95)01376-8 · Cited by 70
Athar, M., Mukhtar, H., Bickers, D. R., Khan, I. U., & Kalyanaraman, B. (1989) · Carcinogenesis · doi:10.1093/carcin/10.8.1499 · Cited by 58
WITHANIA SOMNIFERA ROOT EXTRACT IN THE REGULATION OF LEAD-INDUCED OXIDATIVE DAMAGE IN MALE MOUSE
Chaurasia, S. S., Panda, S., & Kar, A. (2000) · Pharmacological Research · doi:10.1006/phrs.1999.0634 · Cited by 51
Fragmentation of extracellular and intracellular polypeptides by free radicals.
Dean, R. T., Roberts, C. J., & Jessup, W. (1985) · PubMed · Cited by 30
Zafar, K. S., Siddiqui, A., Sayeed, I., Ahmad, M., Saleem, S., & Islam, F. (2003) · Journal of Chemical Neuroanatomy · doi:10.1016/j.jchemneu.2003.08.001 · Cited by 27
Self-citationAgrawal, A. K., Husain, R., Raghubir, R., Kumar, A., & Seth, P. K. (1995) · International Journal of Developmental Neuroscience · doi:10.1016/0736-5748(95)00002-x · Cited by 16
Bartl, R., & Bärtl, C. (2016) · Springer eBooks · doi:10.1007/978-3-032-21885-8_9 · Cited by 6
Research works printed but not linked: 4
- Mishra LC, 2000, Alternive Medicine Reviews, 5, 334
- Panda S, 1997, Indian Journal of Physiology Pharmacology, 41, 424
- Mohandas J, 1984, Cancer Research, 44, 5086
- Cohen G, 1984, Neurotoxicology, 5, 77
Cited by
Sorted by citations
Oxidative Stress, Prooxidants, and Antioxidants: The Interplay
Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., & Dhama, K. (2014) · BioMed Research International · doi:10.1155/2014/761264 · Cited by 1,806
Steroidal Lactones from Withania somnifera, an Ancient Plant for Novel Medicine
Mirjalili, M. H., Moyano, E., Bonfill, M., Cusido, R. M., & Palazón, J. (2009) · Molecules · doi:10.3390/molecules14072373 · Cited by 657
Withania somnifera: an Indian ginseng
Kulkarni, S. K., & Dhir, A. (2008) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2007.09.011 · Cited by 416
Pharmacologic overview of Withania somnifera, the Indian Ginseng
Dar, N. J., Hamid, A., & Ahmad, M. (2015) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-2012-1 · Cited by 391
Self-citationMikulska, P., Malinowska, M., Ignacyk, M., Szustowski, P., Nowak, J., Pesta, K., Szeląg, M., Szklanny, D., Judasz, E., Kaczmarek, G., et al. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15041057 · Cited by 387
Metadata
- Authors
- Ahmad, M., Saleem, S., Ahmad, A. S., Ansari, M. A., Yousuf, S., Hoda, M. N., & Islam, F.
- Affiliations
- Jamia Hamdard (IN)
- Corresponding author
- Islam, Fakhrul
- Venue
- Human & Experimental Toxicology
- Publisher
- SAGE Publications
- Published
- 2005-03
- Volume
- 24
- Issue
- 3
- Pages
- 137-147
- ISSN
- 0960-3271 (ISSN-L)
- 1477-0903
- OpenAlex
- W1977817564
- Cited by
- 227
- References
- 51
- Citations by year
- 2026: 5 · 2025: 14 · 2024: 10 · 2023: 15 · 2022: 14 · 2021: 11 · 2020: 12 · 2019: 5 · 2018: 9 · 2017: 16 · 2016: 17 · 2015: 14 · 2014: 14 · 2013: 17 · 2012: 13
- FWCI
- 12.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human & Experimental Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Health Sciences › Medicine › Clinical Neurology › Parkinson's Disease Mechanisms and Treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Keywords
- Withania somnifera, ashwagandha extract, Parkinson's disease, 6-hydroxydopamine, neuroprotection, oxidative stress, striatum, antioxidant enzymes, lipid peroxidation, dopaminergic neurons, tyrosine hydroxylase, D2 receptor binding, catecholamines
- MeSH terms
- Animals, Behavior, Animal, Brain, Disease Models, Animal, Male, Plant Extracts, Oxidopamine, Rats, Wistar, Neuroprotective Agents, Parkinsonian Disorders, Withania, Rats
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