The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Characterization of overall ceramide species in human stratum corneum
Open accessAbstract
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse CER species, however, have not been comprehensively revealed. The aim of this study was to characterize overall CER species in the SC. First, we constructed 3D multi-mass chromatograms of the overall CER species, based on normal-phase liquid chromatography (NPLC) connected to electrospray ionization-mass spectrometry (ESI-MS) using a gradient elution system and a postcolumn addition of a volatile salt-containing polar solvent. The CERs targeted from the 3D chromatograms were structurally analyzed using NPLC-ESI-tandem mass spectrometry (MS/MS), which resulted in the identification of 342 CER species in the inner forearm SC. This led to the discovery of a new CER class consisting of alpha-hydroxy fatty acid and dihydrosphingosine moieties, in addition to the 10 classes generally known. The results also revealed that those CERs contain long-chain (more than C(18))-containing sphingoids and a great number of isobaric species. These novel results will contribute not only to physiochemical research on CERs in the SC but also to lipidomics approaches to CERs in the skin.
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“In the human SC, 16 ceramide classes other than SD and -hydroxy ceramides have been identified (5)(6)(7).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“Additionally, the fragment ion pattern of ceramides differs depending on their LCB moiety (5).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“Therefore, more than several hundred ceramide species exist in the human SC (5)(6)(7).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“The composition of ceramide classes/species in the human SC has previously been analyzed using LC/MS (5-7, 16, 17, 29, 30).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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Citations
Cites
Functions of Ceramide in Coordinating Cellular Responses to Stress
Hannun, Y. A. (1996) · Science · doi:10.1126/science.274.5294.1855 · Cited by 1,612
Biochemistry of Lipids, Lipoproteins and Membranes
(2021) · Elsevier eBooks · doi:10.1016/c2020-0-00089-7 · Cited by 1,402
Epidermal Lipids, Barrier Function, and Desquamation
Elias, P. M. (1983) · Journal of Investigative Dermatology · doi:10.1038/jid.1983.12 · Cited by 1,356
Imokawa, G., Abe, A., Jin, K., Higaki, Y., Kawashima, M., & Hidano, A. (1991) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12470233 · Cited by 1,040
Ceramide in apoptosis: an overview and current perspectives
Pettus, B. J., Chalfant, C. E., & Hannun, Y. A. (2002) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(02)00331-1 · Cited by 788
Nicolaides, N. (1974) · Science · doi:10.1126/science.186.4158.19 · Cited by 609
Zheng, W., Kollmeyer, J., Symolon, H., Momin, A. A., Munter, E., Wang, E. L., Kelly, S. L., Allegood, J. C., Liu, Y., Peng, Q., et al. (2006) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2006.08.009 · Cited by 561
Ceramide composition of the psoriatic scale
Motta, S., Monti, M., Sesana, S., Caputo, R., Carelli, S., & Ghidoni, R. (1993) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/0925-4439(93)90135-n · Cited by 435
Siegenthaler, U., Laine, A. M., & Polák, L. (1983) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12531034 · Cited by 352
Plasma ceramide and lysophosphatidylcholine inversely correlate with mortality in sepsis patients
Drobnik, W., Liebisch, G., Audebert, F., Fröhlich, D., Glück, T., Vogel, P. M., Rothe, G., & Schmitz, G. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200401-jlr200 · Cited by 350
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Japtok, L., Bäumer, W., & Kleuser, B. (2014) · Allergo Journal · doi:10.1007/s15007-014-0516-x
Increased Presence of Monounsaturated Fatty Acids in the Stratum Corneum of Human Skin
Thakoersing, E. S., van Smeden, J., Mulder, A. A., Vreeken, R. J., Ghalbzouri, A. E., & Bouwstra, J. A. (2013)
Roles of Epidermal Lipids in Barrier Formation
Uchida, Y. (2017) · Oleoscience · doi:10.5650/oleoscience.17.529
Choi, J., Jung, Y. Y., Ha, I. J., Baek, S. H., Zhang, Z., Kim, S.-Y., Hong, Y., Kim, S., Yeum, H., Jeon, S.-R., et al. (2020) · Natural Product Communications · doi:10.1177/1934578x20967866
Analysis of Cosmetic Products in Biological Matrices
Feliczak‐Guzik, A., & Nowak, I. (2021) · Springer eBooks · doi:10.1007/978-3-030-63957-0_46-1
Moss, G. P. (2015) · doi:10.1002/9781118734506.ch1
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Yoon, J., Nishifuji, K., Ishioroshi, S., Ide, K., & Iwasaki, T. (2013) · doi:10.1002/9781118644317.ch13
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Kwatra, S. G. (2020) · MDPI eBooks · doi:10.3390/books978-3-03943-101-4
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Feliczak‐Guzik, A., & Nowak, I. (2022) · Springer eBooks · doi:10.1007/978-3-030-95660-8_46
Kondo, A., Takenaka, Y., Fujiwara, A., Takahashi, S., Kitade-Miyayama, M., Morifuji, M., Kawashima, M., & Ishiguro, N. (2023) · Research Square · doi:10.21203/rs.3.rs-2509454/v1
Application of Magnetic Surfactant in DNA Compaction and Drug Delivery
Alara, J. A., & Alara, O. R. (2023) · ACS symposium series · doi:10.1021/bk-2023-1447.ch003
Khairallah, M. K., Hammam, N. H., Tawfik, N. M., & Dahpy, M. A. (2024) · Journal of The Egyptian Society of Nephrology and Transplantation · doi:10.4103/jesnt.jesnt_29_23
Glycosylinositolphosphoceramide (GIPC) and its Degradation Enzymes in Plants
Tanaka, T. (2025) · Oleoscience · doi:10.5650/oleoscience.25.397
Nakajo, M., Uchino, T., Asada, K., Hatta, I., Yumoto, R., Okada, M., Iwano, Y., Nabekura, Y., Miyazaki, Y., Ikeda, K., et al. (2026) · Cancer Chemotherapy and Pharmacology · doi:10.1007/s00280-026-04915-8
Cho, M., Kim, K., Jeon, S., & Cho, Y. (2026) · Journal of Nutrition and Health · doi:10.4163/jnh.2026.59.3.219
Zhou, Z., Meng, K., Wang, H., Dong, W., Sun, Y. H., & He, Z. (2026) · Chirality · doi:10.1002/chir.70144
Ceramide Metabolic Pathways and Their Regulation in Adolescent Acne Vulgaris
Gou, Y., Wu, H., Wu, X., Dong, G., Li, H., Yuan, H., & Li, L. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27198737
Eichner, A., Hauß, T., Havrišák, T., Demé, B., & Opálka, L. (2026) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2026.127469
Chng, C., Zhang, L., Gupta, S., & Huang, C. (2026) · Membranes · doi:10.3390/membranes16110355
Metadata
- Authors
- Masukawa, Y., Narita, H., Shimizu, E., Kondo, N., Sugai, Y., Oba, T., Homma, R., Ishikawa, J., Takagi, Y., Kitahara, T., Takema, Y., & Kita, K.
- Affiliations
- Kao Corporation (Japan) (JP)
- Corresponding author
- Masukawa, Yoshinori
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 49
- Issue
- 7
- Pages
- 1466-1476
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2159423422
- Cited by
- 322
- References
- 52
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 17 · 2023: 13 · 2022: 17 · 2021: 19 · 2020: 22 · 2019: 18 · 2018: 24 · 2017: 25 · 2016: 24 · 2015: 19 · 2014: 25 · 2013: 30 · 2012: 19
- FWCI
- 4.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Kao Corporation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- ceramide, stratum corneum, skin barrier, dihydrosphingosine, keratinization, normal-phase chromatography, tandem mass spectrometry
- MeSH terms
- Ceramides, Humans, Skin, Mass Spectrometry, Molecular Structure
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