RSV Mediates Pseudomonas aeruginosa Binding to Cystic Fibrosis and Normal Epithelial Cells
Abstract
Cystic fibrosis lung disease typically has a course of exacerbations and remissions, suggesting that external factors like viral infections can influence this course. Clinical data suggest synergism between respiratory syncytial virus (RSV) infections and Pseudomonas aeruginosa in cystic fibrosis (CF) lung disease. We studied the influence of RSV infection on adherence of P. aeruginosa to IB3-1, HEp-2, and A549 epithelial cell monolayers in vitro. RSV infection of epithelial cells as well as simultaneous addition of RSV and P. aeruginosa to noninfected cells both strongly enhanced the pseudomonal adherence to epithelial cells. The increased adherence varied from 1.2- to 8.2-fold in case of previous RSV infection, and from 1.7- to 16.1-fold in case of simultaneous addition. We observed direct binding of RSV to P. aeruginosa, and blocking of RSV with heparin eliminated the effect on increased adherence. This suggests that RSV possibly acts as a coupling agent between P. aeruginosa and epithelial cells. In conclusion, RSV enhances P. aeruginosa infection of respiratory epithelial cells. It suggests a role of specific viral-bacterial interactions in exacerbations of CF lung disease, which could have important implications on prevention and treatment.
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Steroid-based therapies have recognized disappointing or incomplete protection in asthma exacerbations which are of viral etiology.
“Also of note is the recognized disappointing or incomplete protection of steroid-based therapies in asthma exacerbations (64, 197, 295) which are of viral etiology.”
From The airway epithelium: soldier in the fight against respiratory viruses (2011) · cites this paper as [295]
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The airway epithelium: soldier in the fight against respiratory viruses
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- Authors
- van Ewijk, B. E., Wolfs, T. F. W., Aerts, P. C., Van Kessel, K. P. M., Fleer, A., Kimpen, J. L. L., & Van Der Ent, C. K.
- Affiliations
- University Medical Center Utrecht (NL)
- ORCID
- Wolfs, Tom F W
- Venue
- Pediatric Research
- Published
- 2007-04
- Volume
- 61
- Issue
- 4
- Pages
- 398-403
- ISSN
- 0031-3998 (ISSN-L)
- 1530-0447
- OpenAlex
- W2051131055
- Cited by
- 113
- References
- 26
- Citations by year
- 2025: 5 · 2024: 3 · 2023: 5 · 2022: 5 · 2021: 3 · 2020: 7 · 2019: 4 · 2018: 6 · 2017: 9 · 2016: 10 · 2015: 6 · 2014: 5 · 2013: 9 · 2012: 8
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Pediatric Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Universiteit Utrecht
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- respiratory syncytial virus, Pseudomonas aeruginosa, cystic fibrosis, bacterial adhesion, epithelial cells, viral-bacterial interactions, heparin, pulmonary exacerbations, A549 cells, HEp-2 cells
- MeSH terms
- Bacterial Adhesion, Cell Line, Cystic Fibrosis, Epithelial Cells, Humans, Pseudomonas aeruginosa, Respiratory Syncytial Viruses, Cell Line, Tumor
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