Neonatal Gonadectomy and Adult Testosterone Replacement Suggest an Involvement of Limbic Arginine Vasopressin and Androgen Receptors in the Organization of the Hypothalamic-Pituitary-Adrenal Axis
Abstract
Testosterone exposure during critical periods of development exerts major organizing effects on the hypothalamic-pituitary-adrenal (HPA) axis. Here we examined how neonatal gonadectomy (GDX) with or without testosterone treatment during the first week of life alters the HPA response to adult testosterone replacement in 65-d-old male rats. As adults, neonatal GDX rats showed higher levels of plasma corticosterone and Fos activation in medial parvocellular part of the paraventricular nucleus of the hypothalamus under basal conditions and during 30 min of restraint exposure. These responses were normalized with testosterone treatment on postnatal d 1-5 but were not restored with adult testosterone replacement. As adults, neonatal GDX rats also showed a decrease in the number of androgen receptor and arginine vasopressin-positive cells in the bed nucleus of the stria terminalis and in the medial nucleus of the amygdala, and both of these responses were reversed with postnatal testosterone treatment. In stressed and unstressed animals, the number of androgen receptors and arginine vasopressin-expressing neurons in both of these nuclei correlated negatively with corticosterone concentrations in plasma and Fos levels in the paraventricular nucleus. Taken together, our findings suggest that testosterone exposure during the neonatal period primes the adult HPA response to testosterone by altering androgen receptor levels and function within afferent mediators of basal and stress-relat…
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The effects of neonatal gonadectomy on male HPA axis function can be reversed by testosterone treatment during the perinatal period.
“In line with classical mechanisms for sexual differentiation of the rodent brain, the effects of neonatal gonadectomy on male HPA axis function can be reversed by testosterone treatment during the perinatal period (Bingham & Viau, 2008).”
From Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017) · cites this paper as Bingham & Viau, 2008
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- Authors
- Bingham, B., & Viau, V.
- Affiliations
- University of British Columbia (CA)
- ORCID
- Viau, Victor
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2008-04-10
- Volume
- 149
- Issue
- 7
- Pages
- 3581-3591
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2168538548
- Cited by
- 44
- References
- 51
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 1
- FWCI
- 2.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
- Keywords
- testosterone replacement therapy, hypothalamic-pituitary-adrenal axis, vasopressin, androgen receptor, bed nucleus of the stria terminalis, medial amygdala, paraventricular nucleus, corticosterone, Fos expression, stress response, critical period, sex differences
- MeSH terms
- Analysis of Variance, Animals, Animals, Newborn, Arginine Vasopressin, Corticosterone, Corticotropin-Releasing Hormone, Hypothalamo-Hypophyseal System, Immunohistochemistry, Male, Orchiectomy, Pituitary-Adrenal System, Receptors, Androgen, RNA, Messenger, Testosterone, Oncogene Proteins v-fos, Rats, Sprague-Dawley, In Situ Hybridization, Hormone Replacement Therapy, Rats
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