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Cited by 730

The DOI matches its Crossref record.

Osteoclast-Like Cell Formation and its Regulation by Osteotropic Hormones in Mouse Bone Marrow Cultures*

TAKAHASHI, NAOYUKI, YAMANA, HIROMI, YOSHIKI, SHUSAKU, ROODMAN, G. DAVID, MUNDY, GREGORY R., JONES, SHEILA J., BOYDE, ALAN, SUDA, TATSUO·1988·Endocrinology
DOI 10.1210/endo-122-4-1373
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Abstract

We developed a mouse bone marrow culture system to examine the process of osteoclast-like multinucleated cell formation from its progenitors. When mouse marrow cells were cultured for 8 days with 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25-(OH)2D3, 10(-10) to 10(-7) M] or human PTH (1-34) (25-100 ng/ml), tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells formed. No TRACP-positive multinucleated cells appeared in the absence of these hormones. 1 alpha,25-(OH)2D3 and PTH also increased the number of the clusters of TRACP-positive mononuclear cells. Time course studies showed that these TRACP-positive mononuclear cell clusters appeared before the formation of TRACP-positive multinucleated cells, suggesting that the TRACP-positive mononuclear cells are precursors of the multinucleated cells. Salmon calcitonin markedly inhibited the formation of TRACP-positive multinucleated cells but not TRACP-positive mononuclear cell clusters induced by 1 alpha,25-(OH)2D3 or PTH. TRACP-positive mononuclear cells and multinucleated cells were rarely stained for nonspecific esterase, but some mononuclear cells were positively stained for both nonspecific esterase and TRACP. More that 90% of the TRACP-positive mononuclear cell clusters and multinucleated cells were found near colonies of alkaline phosphatase-positive mononuclear cells (possibly osteoblasts). When marrow mononuclear cells were cultured on sperm whale dentine slices in the presence of 1 alpha,25-(OH)2D3 or PTH, numerous resorption lacunae were formed. These results suggest that 1) TRACP-positive multinucleated cells formed in response to osteotropic hormones in mouse marrow cultures satisfy most of the criteria of osteoclasts, and 2) osteoblasts may play an important role in osteoclast formation.

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Metadata

Authors
TAKAHASHI, N., YAMANA, H., YOSHIKI, S., ROODMAN, G. D., MUNDY, G. R., JONES, S. J., BOYDE, A., & SUDA, T.
Venue
Endocrinology
Publisher
The Endocrine Society
Published
1988-04
Volume
122
Issue
4
Pages
1373-1382
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/endo-122-4-1373
OpenAlex
W1977553486
Cited by
730
References
0
FWCI
7.88
Type
journal article
Language
en
Open access
closed

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