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Notochord to endoderm signaling is required for pancreas development
Abstract
The role of the notochord in inducing and patterning adjacent neural and mesodermal tissues is well established. We provide evidence that the notochord is also required for one of the earliest known steps in the development of the pancreas, an endodermally derived organ. At a developmental stage in chick embryos when the notochord touches the endoderm, removal of notochord eliminates subsequent expression of several markers of dorsal pancreas bud development, including insulin, glucagon and carboxypeptidase A. Pancreatic gene expression can be initiated and maintained in prepancreatic chick endoderm grown in vitro with notochord. Non-pancreatic endoderm, however, does not express pancreatic genes when recombined with the same notochord. The results suggest that the notochord provides a permissive signal to endoderm to specify pan-creatic fate in a stepwise manner.
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“FGF2 and Activin, secreted by the notochord, lead to the expression of pancreatic markers by repressing expression of Shh in pancreatic endoderm [15-19].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [18]
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Metadata
- Authors
- Kim, S. K., Hebrok, M., & Melton, D. A.
- Affiliations
- Howard Hughes Medical Institute (US)
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1997-11-01
- Volume
- 124
- Issue
- 21
- Pages
- 4243-4252
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1844743435
- Cited by
- 405
- References
- 59
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 3 · 2023: 8 · 2022: 1 · 2021: 4 · 2020: 6 · 2019: 4 · 2018: 10 · 2017: 7 · 2016: 9 · 2015: 9 · 2014: 7 · 2013: 12 · 2012: 14
- FWCI
- 6.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Health Sciences › Medicine › Oncology › Renal and related cancers
- Keywords
- notochord, endoderm, pancreatic development, dorsal pancreas, insulin, glucagon, carboxypeptidase A, chick embryo, in vitro recombination
- MeSH terms
- Animals, Cells, Cultured, Chick Embryo, Embryonic Induction, Endoderm, Nerve Tissue Proteins, Notochord, Pancreas, Transcription Factors, Signal Transduction, Biomarkers, Trans-Activators, Homeodomain Proteins, Gene Expression Regulation, Developmental, LIM-Homeodomain Proteins
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