The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Expression of neurogenin3 reveals an islet cell precursor population in the pancreas
Open accessAbstract
Differentiation of early gut endoderm cells into the endocrine cells forming the pancreatic islets of Langerhans depends on a cascade of gene activation events controlled by transcription factors including the basic helix-loop-helix (bHLH) proteins. To delineate this cascade, we began by establishing the position of neurogenin3, a bHLH factor found in the pancreas during fetal development. We detect neurogenin3 immunoreactivity transiently in scattered ductal cells in the fetal mouse pancreas, peaking at embryonic day 15.5. Although not detected in cells expressing islet hormones or the islet transcription factors Isl1, Brn4, Pax6 or PDX1, neurogenin3 is detected along with early islet differentiation factors Nkx6.1 and Nkx2.2, establishing that it is expressed in immature cells in the islet lineage. Analysis of transcription factor-deficient mice demonstrates that neurogenin3 expression is not dependent on neuroD1/BETA2, Mash1, Nkx2.2, Nkx6.1, or Pax6. Furthermore, early expression of neurogenin3 under control of the Pdx1 promoter is alone sufficient to drive early and ectopic differentiation of islet cells, a capability shared by the pancreatic bHLH factor, neuroD1/BETA2, but not by the muscle bHLH factor, MyoD. However, the islet cells produced in these transgenic experiments are overwhelmingly α cells, suggesting that factors other than the bHLH factors are required to deviate from a default α cell fate. These data support a model in which neurogenin3 acts upstream of other islet differentiation factors, initiating the differentiation of endocrine cells, but switching off prior to final differentiation. The ability to uniquely identify islet cell precursors by neurogenin3 expression allows us to determine the position of other islet transcription factors in the differentiation cascade and to propose a map for the islet cell differentiation pathway.
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Cites
Manipulating the mouse embryo: A laboratory manual
Hogan, B. L. M., Costantini, F. D., & Lacy, E. (1986) · Cited by 4,460
Expression of a single transfected cDNA converts fibroblasts to myoblasts
Davis, R. L., Weintraub, H., & Lassar, A. B. (1987) · Cell · doi:10.1016/0092-8674(87)90585-x · Cited by 3,385
Manipulating the mouse embryo: A laboratory manual
Johnson, M. (1986) · Trends in Genetics · doi:10.1016/0168-9525(86)90275-1 · Cited by 2,245
PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum
Offield, M. F., Jetton, T. L., Labosky, P. A., Ray, M., Stein, R. W., Magnuson, M. A., Hogan, B. L. M., & Wright, C. V. E. (1996) · Development · doi:10.1242/dev.122.3.983 · Cited by 1,513
neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas
Gradwohl, G., Dierich, A., LeMeur, M., & Guillemot, F. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.4.1607 · Cited by 1,439
Notch signalling controls pancreatic cell differentiation
Apelqvist, Å., Li, H., Sommer, L., Beatus, P., Anderson, D. J., Honjo, T., de Angelis, M. H., Lendahl, U., & Edlund, H. (1999) · Nature · doi:10.1038/23716 · Cited by 1,159
Self-citationGuillemot, F., Lo, L.-C., Johnson, J. E., Auerbach, A., Anderson, D. J., & Joyner, A. L. (1993) · Cell · doi:10.1016/0092-8674(93)90381-y · Cited by 1,033
Self-citationConversion of Xenopus Ectoderm into Neurons by NeuroD, a Basic Helix-Loop-Helix Protein
Lee, J. E., Hollenberg, S. M., Snider, L., Turner, D. L., Lipnick, N., & Weintraub, H. (1995) · Science · doi:10.1126/science.7754368 · Cited by 1,023
Self-citationNaya, F. J., Huang, H.-P., Qiu, Y., Mutoh, H., DeMayo, F. J., Leiter, A. B., & Tsai, M.-J. (1997) · Genes & Development · doi:10.1101/gad.11.18.2323 · Cited by 1,021
Developmental biology of the pancreas
Slack, J. M. W. (1995) · Development · doi:10.1242/dev.121.6.1569 · Cited by 954
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Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells
D'Amour, K. A., Bang, A. G., Eliazer, S., Kelly, O. G., Agulnick, A. D., Smart, N. G., Moorman, M. A., Kroon, E., Carpenter, M. K., & Baetge, E. E. (2006) · Nature Biotechnology · doi:10.1038/nbt1259 · Cited by 1,915
Gu, G., Dubauskaite, J., & Melton, D. A. (2002) · Development · doi:10.1242/dev.129.10.2447 · Cited by 1,518
Differentiation of Embryonic Stem Cells to Insulin-Secreting Structures Similar to Pancreatic Islets
Lumelsky, N., Blondel, O., Laeng, P., Velasco, I., Ravin, R., & McKay, R. (2001) · Science · doi:10.1126/science.1058866 · Cited by 1,453
β Cells Can Be Generated from Endogenous Progenitors in Injured Adult Mouse Pancreas
Xu, X., D'Hoker, J., Stangé, G., Bonné, S., De Leu, N., Xiao, X., Van De Casteele, M., Mellitzer, G., Ling, Z., Pipeleers, D., et al. (2008) · Cell · doi:10.1016/j.cell.2007.12.015 · Cited by 988
Opposing actions of Arx and Pax4 in endocrine pancreas development
Collombat, P., Mansouri, A., Hecksher-Sørensen, J., Serup, P., Krull, J., Gradwohl, G., & Gruss, P. (2003) · Genes & Development · doi:10.1101/gad.269003 · Cited by 600
A Multipotent Progenitor Domain Guides Pancreatic Organogenesis
Zhou, Q., Law, A. C., Rajagopal, J., Anderson, W. J., Gray, P. A., & Melton, D. A. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.06.001 · Cited by 537
Developmental biology of the pancreas: A comprehensive review
Gittes, G. K. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2008.10.024 · Cited by 451
Tan, S. Y., Mei Wong, J. L., Sim, Y. J., Wong, S. S., Mohamed Elhassan, S. A., Tan, S. H., Ling Lim, G. P., Rong Tay, N. W., Annan, N. C., Bhattamisra, S. K., et al. (2019) · Diabetes & Metabolic Syndrome: Clinical Research & Reviews · doi:10.1016/j.dsx.2018.10.008 · Cited by 445
Blyszczuk, P., Czyz, J., Kania, G., Wagner, M., Roll, U., St-Onge, L., & Wobus, A. M. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237371100 · Cited by 444
MicroRNA Expression Is Required for Pancreatic Islet Cell Genesis in the Mouse
Lynn, F. C., Skewes-Cox, P., Kosaka, Y., McManus, M. T., Harfe, B. D., & German, M. S. (2007) · Diabetes · doi:10.2337/db07-0175 · Cited by 351
Self-citationMinireview: Development and Differentiation of Gut Endocrine Cells
Schonhoff, S. E., Giel-Moloney, M., & Leiter, A. B. (2004) · Endocrinology · doi:10.1210/en.2004-0051 · Cited by 322
Grapin-Botton, A., Majithia, A. R., & Melton, D. A. (2001) · Genes & Development · doi:10.1101/gad.846001 · Cited by 296
Schonhoff, S. E., Giel-Moloney, M., & Leiter, A. B. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2004.03.013 · Cited by 284
Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas
Bort, R., Martinez-Barbera, J. P., Beddington, R. S. P., & Zaret, K. S. (2004) · Development · doi:10.1242/dev.00965 · Cited by 267
Neurogenin3 delineates the earliest stages of spermatogenesis in the mouse testis
Yoshida, S., Takakura, A., Ohbo, K., Abe, K., Wakabayashi, J., Yamamoto, M., Suda, T., & Nabeshima, Y.-I. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2004.01.036 · Cited by 272
Molecular regulation of pancreatic β-cell mass development, maintenance, and expansion
Ackermann, A. M., & Gannon, M. (2007) · Journal of Molecular Endocrinology · doi:10.1677/jme-06-0053 · Cited by 262
Regulation of the Pancreatic Pro-Endocrine Gene Neurogenin3
Lee, J. C., Smith, S. B., Watada, H., Lin, J., Scheel, D., Wang, J., Mirmira, R. G., & German, M. S. (2001) · Diabetes · doi:10.2337/diabetes.50.5.928 · Cited by 257
Self-citationCollombat, P., Hecksher-Sørensen, J., Krull, J., Berger, J., Riedel, D., Herrera, P. L., Serup, P., & Mansouri, A. (2007) · Journal of Clinical Investigation · doi:10.1172/jci29115 · Cited by 256
Guz, Y., Nasir, I., & Teitelman, G. (2001) · Endocrinology · doi:10.1210/endo.142.11.8501 · Cited by 254
Global expression analysis of gene regulatory pathways during endocrine pancreatic development
Gu, G., Wells, J. M., Dombkowski, D., Preffer, F., Aronow, B., & Melton, D. A. (2004) · Development · doi:10.1242/dev.00921 · Cited by 229
Artifactual Insulin Release From Differentiated Embryonic Stem Cells
Hansson, M., Tonning, A., Frandsen, U., Petri, A., Rajagopal, J., Englund, M. C. O., Heller, R. S., Håkansson, J., Fleckner, J., Sköld, H. N., et al. (2004) · Diabetes · doi:10.2337/diabetes.53.10.2603 · Cited by 225
Oliver-Krasinski, J. M., Kasner, M. T., Yang, J., Crutchlow, M. F., Rustgi, A. K., Kaestner, K. H., & Stoffers, D. A. (2009) · Journal of Clinical Investigation · doi:10.1172/jci37028 · Cited by 177
Dumortier, O., Blondeau, B., Duvillié, B., Reusens, B., Bréant, B., & Remacle, C. (2007) · Diabetologia · doi:10.1007/s00125-007-0811-0 · Cited by 124
Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells
Trott, J., Tan, E. K., Ong, S., Titmarsh, D. M., Denil, S. L. I. J., Giam, M., Wong, C. K., Wang, J., Shboul, M., Eio, M., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.05.019 · Cited by 77
Stem cells and pancreatic differentiation in vitro
Blyszczuk, P., & Wobus, A. M. (2004) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2004.03.023 · Cited by 31
Metadata
- Authors
- Schwitzgebel, V. M., Scheel, D. W., Conners, J. R., Kalamaras, J., Lee, J. E., Anderson, D. J., Sussel, L., Johnson, J. D., & German, M. S.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2000-08-15
- Volume
- 127
- Issue
- 16
- Pages
- 3533-3542
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2110447217
- Cited by
- 678
- References
- 64
- FWCI
- 26.29
- Type
- journal article
- Language
- en
- Open access
- green
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