Transcription elongation rate has a tissue-specific impact on alternative cleavage and polyadenylation in Drosophila melanogaster
Open accessAbstract
Alternative polyadenylation (APA) is a mechanism that generates multiple mRNA isoforms with different 3′UTRs and/or coding sequences from a single gene. Here, using 3′ region extraction and deep sequencing (3′READS), we have systematically mapped cleavage and polyadenylation sites (PASs) in Drosophila melanogaster, expanding the total repertoire of PASs previously identified for the species, especially those located in A-rich genomic sequences. Cis-element analysis revealed distinct sequence motifs around fly PASs when compared to mammalian ones, including the greater enrichment of upstream UAUA elements and the less prominent presence of downstream UGUG elements. We found that over 75% of mRNA genes in Drosophila melanogaster undergo APA. The head tissue tends to use distal PASs when compared to the body, leading to preferential expression of APA isoforms with long 3′UTRs as well as with distal terminal exons. The distance between the APA sites and intron location of PAS are important parameters for APA difference between body and head, suggesting distinct PAS selection contexts. APA analysis of the RpII215C4 mutant strain, which harbors a mutant RNA polymerase II (RNAPII) with a slower elongation rate, revealed that a 50% decrease in transcriptional elongation rate leads to a mild trend of more usage of proximal, weaker PASs, both in 3′UTRs and in introns, consistent with the “first come, first served” model of APA regulation. However, this trend was not observed in the head, suggesting a different regulatory context in neuronal cells. Together, our data expand the PAS collection for Drosophila melanogaster and reveal a tissue-specific effect of APA regulation by RNAPII elongation rate.
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A slow RNA Pol II mutant shows a tissue-specific effect on alternative polyadenylation in Drosophila head or body.
“In addition, a slow RNA Pol II mutant shows a tissue‐specific effect on alternative polyadenylation as it affects poly(A) site usage differentially in the Drosophila head or body (Liu et al, 2017).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Liu et al, 2017
On several genes possessing alternative poly(A) sites, a slow RNA Pol II mutant shows a switch to upstream poly(A) sites, while a fast mutant shows a slight downstream switch.
“On several genes possessing alternative poly(A) sites, a slow RNA Pol II mutant shows a switch to the use of upstream poly(A) sites (Pinto et al, 2011; Liu et al, 2017; Geisberg et al, 2020; Yague‐Sanz et al, 2020), while a fast RNA Pol II mutant shows a slight downstream switch in poly(A) site usage (Geisberg et al, 2020).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Liu et al, 2017
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Cites
Fast gapped-read alignment with Bowtie 2
Langmead, B., & Salzberg, S. L. (2012) · Nature Methods · doi:10.1038/nmeth.1923 · Cited by 56,400 · see citing works
Alternative isoform regulation in human tissue transcriptomes
Wang, E. T., Sandberg, R., Luo, S., Khrebtukova, I., Zhang, L., Mayr, C., Kingsmore, S. F., Schroth, G. P., & Burge, C. B. (2008) · Nature · doi:10.1038/nature07509 · Cited by 5,181 · see citing works
Mayr, C., & Bartel, D. P. (2009) · Cell · doi:10.1016/j.cell.2009.06.016 · Cited by 1,613 · see citing works
Detecting differential usage of exons from RNA-seq data
Anders, S., Reyes, A., & Huber, W. (2012) · Genome Research · doi:10.1101/gr.133744.111 · Cited by 1,545 · see citing works
Sandberg, R., Neilson, J. R., Sarma, A., Sharp, P. A., & Burge, C. B. (2008) · Science · doi:10.1126/science.1155390 · Cited by 1,349 · see citing works
Alternative polyadenylation of mRNA precursors
Tian, B., & Manley, J. L. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.116 · Cited by 1,179 · see citing works
Self-citationZhao, J., Hyman, L. E., & Moore, C. L. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.2.405-445.1999 · Cited by 1,024 · see citing works
A large-scale analysis of mRNA polyadenylation of human and mouse genes
Tian, B. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki158 · Cited by 944 · see citing works
Self-citationAlternative cleavage and polyadenylation: extent, regulation and function
Elkon, R., Ugalde, A. P., & Agami, R. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3482 · Cited by 805 · see citing works
Mechanisms and Consequences of Alternative Polyadenylation
Di Giammartino, D. C., Nishida, K., & Manley, J. L. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.08.017 · Cited by 737 · see citing works
A Slow RNA Polymerase II Affects Alternative Splicing In Vivo
de la Mata, M., Alonso, C. R., Kadener, S., Fededa, J. P., Blaustein, M., Pelisch, F., Cramer, P., Bentley, D., & Kornblihtt, A. R. (2003) · Molecular Cell · doi:10.1016/j.molcel.2003.08.001 · Cited by 683 · see citing works
A quantitative atlas of polyadenylation in five mammalsPreprint
Derti, A., Garrett-Engele, P. W., MacIsaac, K. D., Stevens, R. C., Sriram, S., Chen, R., Rohl, C. A., Johnson, J. M., & Babak, T. (2012) · Genome Research · doi:10.1101/gr.132563.111 · Cited by 642 · see citing works
Ending the message: poly(A) signals then and nowPreprint
Proudfoot, N. J. (2011) · Genes & Development · doi:10.1101/gad.17268411 · Cited by 607 · see citing works
Formation, regulation and evolution of Caenorhabditis elegans 3′UTRs
Jan, C. H., Friedman, R. C., Ruby, J. G., & Bartel, D. P. (2010) · Nature · doi:10.1038/nature09616 · Cited by 482 · see citing works
Analysis of alternative cleavage and polyadenylation by 3′ region extraction and deep sequencing
Hoque, M., Ji, Z., Zheng, D., Luo, W., Li, W., You, B., Park, J. Y., Yehia, G., & Tian, B. (2012) · Nature Methods · doi:10.1038/nmeth.2288 · Cited by 442 · see citing works
Self-citationFlavell, S. W., Kim, T.-K., Gray, J. M., Harmin, D. A., Hemberg, M., Hong, E. J., Markenscoff-Papadimitriou, E., Bear, D. M., & Greenberg, M. E. (2008) · Neuron · doi:10.1016/j.neuron.2008.11.029 · Cited by 440 · see citing works
Complex and dynamic landscape of RNA polyadenylation revealed by PAS-Seq
Shepard, P. J., Choi, E., Lu, J., Flanagan, L. A., Hertel, K. J., & Shi, Y. (2011) · RNA · doi:10.1261/rna.2581711 · Cited by 432 · see citing works
Transcriptional termination in mammals: Stopping the RNA polymerase II juggernaut
Proudfoot, N. J. (2016) · Science · doi:10.1126/science.aad9926 · Cited by 413 · see citing works
Ozsolak, F., Kapranov, P., Foissac, S., Kim, S. W., Fishilevich, E., Monaghan, A. P., John, B., & Milos, P. M. (2010) · Cell · doi:10.1016/j.cell.2010.11.020 · Cited by 400 · see citing works
Ubiquitously transcribed genes use alternative polyadenylation to achieve tissue-specific expressionPreprint
Lianoglou, S., Garg, V., Yang, J. L., Leslie, C. S., & Mayr, C. (2013) · Genes & Development · doi:10.1101/gad.229328.113 · Cited by 389 · see citing works
Evolution and Biological Roles of Alternative 3′UTRs
Mayr, C. (2015) · Trends in Cell Biology · doi:10.1016/j.tcb.2015.10.012 · Cited by 329 · see citing works
Extensive alternative polyadenylation during zebrafish developmentPreprint
Ulitsky, I., Shkumatava, A., Jan, C. H., Subtelny, A. O., Koppstein, D., Bell, G. W., Sive, H. L., & Bartel, D. P. (2012) · Genome Research · doi:10.1101/gr.139733.112 · Cited by 326 · see citing works
Wu, X., Liu, M., Downie, A. B., Liang, C., Ji, G., Li, Q. Q., & Hunt, A. G. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1019732108 · Cited by 303 · see citing works
Biased alternative polyadenylation in human tissues
Zhang, H., Lee, J. Y., & Tian, B. (2005) · Genome biology · doi:10.1186/gb-2005-6-12-r100 · Cited by 292 · see citing works
Self-citationPre-mRNA splicing is facilitated by an optimal RNA polymerase II elongation rate
Fong, N., Kim, H., Zhou, Y., Ji, X., Qiu, J., Saldi, T., Diener, K., Jones, K., Fu, X.-D., & Bentley, D. L. (2014) · Genes & Development · doi:10.1101/gad.252106.114 · Cited by 287 · see citing works
Fu, Y., Sun, Y., Li, Y., Li, J., Rao, X., Chen, C., & Xu, A. (2011) · Genome Research · doi:10.1101/gr.115295.110 · Cited by 270 · see citing works
Ji, Z., & Tian, B. (2009) · PLoS ONE · doi:10.1371/journal.pone.0008419 · Cited by 264 · see citing works
Self-citationLi, W., You, B., Hoque, M., Zheng, D., Luo, W., Ji, Z., Park, J. Y., Gunderson, S., Kalsotra, A., Manley, J. L., et al. (2015) · PLoS Genetics · doi:10.1371/journal.pgen.1005166 · Cited by 263 · see citing works
Self-citationThe end of the message: multiple protein–RNA interactions define the mRNA polyadenylation site
Shi, Y., & Manley, J. L. (2015) · Genes & Development · doi:10.1101/gad.261974.115 · Cited by 249 · see citing works
HU, J., Lutz, C. S., Wilusz, J., & Tian, B. (2005) · RNA · doi:10.1261/rna.2107305 · Cited by 249 · see citing works
Self-citationThe Landscape of C. elegans 3′UTRs
Mangone, M., Manoharan, A. P., Thierry‐Mieg, D., Thierry‐Mieg, J., Han, T., Mackowiak, S. D., Mis, E. K., Zegar, C., Gutwein, M. R., Khivansara, V., et al. (2010) · Science · doi:10.1126/science.1191244 · Cited by 247 · see citing works
Orphan nuclear receptor TR3 acts in autophagic cell death via mitochondrial signaling pathway
Wang, W.-J., Wang, Y., Chen, H., Xing, Y.-Z., Li, F.-W., Zhang, Q., Zhou, B., Zhang, H., Zhang, J., Bian, X., et al. (2013) · Nature Chemical Biology · doi:10.1038/nchembio.1406 · Cited by 245 · see citing works
Signals for pre‐mRNA cleavage and polyadenylation
Tian, B., & Graber, J. H. (2011) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.116 · Cited by 237 · see citing works
Self-citationGlobal Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila
Smibert, P., Miura, P., Westholm, J. O., Shenker, S., May, G. E., Duff, M. O., Zhang, D., Eads, B. D., Carlson, J., Brown, J. B., et al. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.01.001 · Cited by 226 · see citing works
Fong, N., Brannan, K., Erickson, B., Kim, H., Cortazar, M. A., Sheridan, R. M., Nguyen, T., Karp, S., & Bentley, D. L. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.09.026 · Cited by 217 · see citing works
Alternative polyadenylation: New insights from global analyses
Shi, Y. (2012) · RNA · doi:10.1261/rna.035899.112 · Cited by 212 · see citing works
Direct sequencing of Arabidopsis thaliana RNA reveals patterns of cleavage and polyadenylation
Sherstnev, A., Duc, C., Cole, C., Zacharaki, V., Hornyik, C., Ozsolak, F., Milos, P. M., Barton, G. J., & Simpson, G. G. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2345 · Cited by 157 · see citing works
Singh, P., Alley, T. L., Wright, S., Kamdar, S. J., Schott, W. H., Wilpan, R. Y., Mills, K. D., & Graber, J. H. (2009) · Cancer Research · doi:10.1158/0008-5472.can-09-2236 · Cited by 156 · see citing works
Alternative mRNA polyadenylation in eukaryotes: an effective regulator of gene expression
Lutz, C. S., & Moreira, A. (2010) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.47 · Cited by 145 · see citing works
Self-citationRNA polymerase II kinetics in polo polyadenylation signal selection
Pinto, P. A. B., Henriques, T., Freitas, M. O., Martins, T., Domingues, R. G., Wyrzykowska, P. S., Coelho, P. A., Carmo, A. M., Sunkel, C. E., Proudfoot, N. J., et al. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.156 · Cited by 136 · see citing works
Self-citationCDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II–transcribed genes
Laitem, C., Zaborowska, J., Isa, N. F., Kufs, J. E., Dienstbier, M., & Murphy, S. (2015) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.3000 · Cited by 135 · see citing works
Systematic variation in mRNA 3′-processing signals during mouse spermatogenesis
Liu, D., Brockman, J. M., Dass, B., Hutchins, L. N., Singh, P., McCarrey, J. R., MacDonald, C. C., & Graber, J. H. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl919 · Cited by 130 · see citing works
Probabilistic prediction of Saccharomyces cerevisiae mRNA 3'-processing sites
Graber, J. H. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.8.1851 · Cited by 122 · see citing works
Alternative Cleavage and Polyadenylation during Colorectal Cancer Development
Morris, A. R., Bos, A., Diosdado, B., Rooijers, K., Elkon, R., Bolijn, A. S., Carvalho, B. G., Meijer, G. A., & Agami, R. (2012) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-12-0543 · Cited by 122 · see citing works
de la Mata, M., Lafaille, C., & Kornblihtt, A. R. (2010) · RNA · doi:10.1261/rna.1993510 · Cited by 115 · see citing works
Alternative polyadenylation and gene expression regulation in plants
Xing, D., & Li, Q. Q. (2010) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.59 · Cited by 110 · see citing works
ELAV mediates 3′ UTR extension in the Drosophila nervous systemPreprint
Hilgers, V., Lemke, S. B., & Levine, M. (2012) · Genes & Development · doi:10.1101/gad.199653.112 · Cited by 101 · see citing works
An efficient method for genome-wide polyadenylation site mapping and RNA quantification
Wilkening, S., Pelechano, V., Järvelin, A. I., Tekkedil, M. M., Anders, S., Beneš, V., & Steinmetz, L. M. (2013) · Nucleic Acids Research · doi:10.1093/nar/gks1249 · Cited by 101 · see citing works
Association of IA-2 autoantibodies with HLA DR4 phenotypes in IDDM
Genovese, S., Bonfanti, R., Bazzigaluppi, E., Lampasona, V., Benazzi, E., Bosi, E., Chiumello, G., & Bonifacio, E. E. (1996) · Diabetologia · doi:10.1007/bf02658510 · Cited by 85 · see citing works
Li, W., Park, J. Y., Zheng, D., Hoque, M., Yehia, G., & Tian, B. (2016) · BMC Biology · doi:10.1186/s12915-016-0229-6 · Cited by 84 · see citing works
Self-citationmRNA 3′-UTR shortening is a molecular signature of mTORC1 activation
Chang, J.-W., Zhang, W., Yeh, H.-S., de Jong, E. P., Jun, S., Kim, K., Bae, S. S., Beckman, K. B., Hwang, T. H., Kim, K.-S., et al. (2015) · Nature Communications · doi:10.1038/ncomms8218 · Cited by 82 · see citing works
Haenni, S., Ji, Z., Hoque, M., Rust, N., Sharpe, H., Eberhard, R., Browne, C., Hengartner, M. O., Mellor, J., Tian, B., et al. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks282 · Cited by 71 · see citing works
Self-citation3′READS+, a sensitive and accurate method for 3′ end sequencing of polyadenylated RNA
Zheng, D., Liu, X., & Tian, B. (2016) · RNA · doi:10.1261/rna.057075.116 · Cited by 71 · see citing works
Self-citationRNA-Binding Proteins in Regulation of Alternative Cleavage and Polyadenylation
Zheng, D., & Tian, B. (2014) · Advances in experimental medicine and biology · doi:10.1007/978-1-4939-1221-6_3 · Cited by 68 · see citing works
Self-citationSimilarities and differences of polyadenylation signals in human and fly
Retelska, D., Iseli, C., Bücher, P., Jongeneel, C. V., & Naef, F. (2006) · BMC Genomics · doi:10.1186/1471-2164-7-176 · Cited by 68 · see citing works
Mata, J. (2013) · RNA Biology · doi:10.4161/rna.25758 · Cited by 51 · see citing works
The IA-2 gene family: homologs in Caenorhabditis elegans, Drosophila and zebrafish
Cai, T., Krause, M., Odenwald, W. F., Toyama, R., & Notkins, A. L. (2001) · Diabetologia · doi:10.1007/s001250051583 · Cited by 48 · see citing works
Drosophila RNA Polymerase II Mutants That Affect Transcription Elongation
Chen, Y., Chafin, D. R., Price, D. H., & Greenleaf, A. L. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.11.5993 · Cited by 44 · see citing works
Chen, Y., Weeks, J., Mortin, M. A., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.7.4214-4222.1993 · Cited by 43 · see citing works
Genome-wide analysis of poly(A) site selection in Schizosaccharomyces pombe
Schlackow, M., Marguerat, S., Proudfoot, N. J., Bähler, J., Radek Erban, A., & Gullerová, M. (2013) · RNA · doi:10.1261/rna.040675.113 · Cited by 37 · see citing works
Williams, A. J. K., Aitken, R. J., Chandler, M. A.-M., Gillespie, K. M., Lampasona, V., & Bingley, P. J. (2008) · Diabetologia · doi:10.1007/s00125-008-1047-3 · Cited by 34 · see citing works
Novel upstream and downstream sequence elements contribute to polyadenylation efficiency
Darmon, S. K., & Lutz, C. S. (2012) · RNA Biology · doi:10.4161/rna.21957 · Cited by 27 · see citing works
Chen, Y., Weeks, J. R., Mortin, M. A., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.7.4214 · Cited by 26 · see citing works
Accurate Mapping of Cleavage and Polyadenylation Sites by 3′ Region Extraction and Deep Sequencing
Hoque, M., Li, W., & Tian, B. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-971-0_10 · Cited by 24 · see citing works
Self-citationslowmo is required for Drosophila germline proliferation
Reeve, S. P., Çarhan, A., Dee, C. T., et al. (2007) · genesis · doi:10.1002/dvg.20265 · Cited by 7 · see citing works
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Dubbury, S. J., Boutz, P. L., & Sharp, P. A. (2018) · Nature · doi:10.1038/s41586-018-0758-y · Cited by 318 · see citing works
RNA polymerase II speed: a key player in controlling and adapting transcriptome composition
Muniz, L., Nicolas, E., & Trouche, D. (2021) · The EMBO Journal · doi:10.15252/embj.2020105740 · Cited by 203 · see citing works
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Hou, L., Wang, Y., Liu, Y., Zhang, N., Shamovsky, I., Nudler, E., Tian, B., & Dynlacht, B. D. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1904324116 · Cited by 129 · see citing works
Self-citationTranscription elongation rate affects nascent histone pre-mRNA folding and 3′ end processing
Saldi, T., Fong, N., & Bentley, D. L. (2018) · Genes & Development · doi:10.1101/gad.310896.117 · Cited by 66 · see citing works
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- Authors
- Liu, X., Freitas, J., Zheng, D., Oliveira, M. S., Hoque, M., Martins, T., Henriques, T., Tian, B., & Moreira, A.
- Affiliations
- Rutgers New Jersey Medical School (US)
- Universidade do Porto (PT)
- i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (PT)
- Corresponding author
- Tian, Bin
- Moreira, Alexandra
- ORCID
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2017-08-29
- Volume
- 23
- Issue
- 12
- Pages
- 1807-1816
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- PMC
- PMC5689002
- OpenAlex
- W2751688575
- Cited by
- 67
- References
- 65
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 9 · 2023: 8 · 2022: 6 · 2021: 10 · 2020: 12 · 2019: 9 · 2018: 4 · 2012: 1
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- RNA (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Institutes of Health (GM084089, Norte-01-0145-FEDER-000008)
- Porto Neurosciences and Neurologic Disease Research Initiative
- Norte Portugal Regional Operational Programme (NORTE 2020, FCOMP-01-0124-FEDER-028252, SFRH/BD/102002/2014)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- alternative polyadenylation, RNA polymerase II, Drosophila melanogaster, tissue-specific gene regulation, cis-regulatory elements
- MeSH terms
- Animals, Drosophila melanogaster, Male, RNA Polymerase II, Gene Expression Regulation, Fungal, Alternative Splicing, 3' Untranslated Regions, Polyadenylation, Animals, Genetically Modified, High-Throughput Nucleotide Sequencing, Transcription Elongation, Genetic
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