The DOI matches its Crossref record; 46 of the 47 references it cites were found in a registry or an index.
Glucocorticoid-Treated Mice Have Localized Changes in Trabecular Bone Material Properties and Osteocyte Lacunar Size That Are Not Observed in Placebo-Treated or Estrogen-Deficient Mice
Open accessAbstract
UNLABELLED: This study compares changes in bone microstructure in 6-month-old male GC-treated and female ovariectomized mice to their respective controls. In addition to a reduction in trabecular bone volume, GC treatment reduced bone mineral and elastic modulus of bone adjacent to osteocytes that was not observed in control mice nor estrogen-deficient mice. These microstructural changes in combination with the macrostructural changes could amplify the bone fragility in this metabolic bone disease. INTRODUCTION: Patients with glucocorticoid (GC)-induced secondary osteoporosis tend to fracture at higher bone mineral densities than patients with postmenopausal osteoporosis. This suggests that GCs may alter bone material properties in addition to BMD and bone macrostructure. MATERIALS AND METHODS: Changes in trabecular bone structure, elastic modulus, and mineral to matrix ratio of the fifth lumbar vertebrae was assessed in prednisolone-treated mice and placebo-treated controls for comparison with estrogen-deficient mice and sham-operated controls. Compression testing of the third lumbar vertebrae was performed to assess whole bone strength. RESULTS: Significant reductions in trabecular bone volume and whole bone strength occurred in both prednisolone-treated and estrogen-deficient mice compared with controls after 21 days (p < 0.05). The average elastic modulus over the entire surface of each trabecula was similar in all the experimental groups. However, localized changes within the trabeculae in areas surrounding the osteocyte lacunae were observed only in the prednisolone-treated mice. The size of the osteocyte lacunae was increased, reduced elastic modulus around the lacunae was observed, and a "halo" of hypomineralized bone surrounding the lacunae was observed. This was associated with reduced (nearly 40%) mineral to matrix ratio determined by Raman microspectroscopy. These localized changes in elastic modulus and bone mineral to matrix ratio were not observed in the other three experimental groups. CONCLUSIONS: Based on these results, it seems that GCs may have direct effects on osteocytes, resulting in a modification of their microenvironment. These changes, including an enlargement of their lacunar space and the generation of a surrounding sphere of hypomineralized bone, seem to produce highly localized changes in bone material properties that may influence fracture risk.
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In the citing paper
“Prolonged GC treatments further worsen the bone quality [35, 55]”
From Prevention of glucocorticoid induced bone changes with beta-ecdysone (2015) · cites this paper as [35]
In the citing paper
“This finding again suggests the adverse effect of GC on the cortical bone quality may not correlate with whole bone mass or bone mineral density [35, 59].”
From Prevention of glucocorticoid induced bone changes with beta-ecdysone (2015) · cites this paper as [35]
In the citing paper
“Compared to the other studies that we and others have published on GIOP that used skeletal matured mice, we found GC excess in the young growing have more profound inhibition on periosteal expansion with similar degree of trabecular bone loss [35, 41].”
From Prevention of glucocorticoid induced bone changes with beta-ecdysone (2015) · cites this paper as [35]
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Cites
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Bone Histomorphometry : Standardization of Nomenclature, Symbols, and Units
PARFITT, A. M., Drezner, M. K., Glorieux, F. H., Kanis, J. А., Malluche, H. H., Meunier, P. J., Ott, S. M., & Recker, R. R. (1987) · Journal of Bone and Mineral Research · Cited by 3,258
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“We found greater numbers of apoptotic osteocytes in prednisolone-treated mice compared with the controls, but substantially less than reported by Weinstein et al.(14,25) Bellido et al.(36) reported in an in vitro study that GCs induced apoptosis of osteocytes.”
BackgroundMethodInfluentialPARFITT, A. M., Mathews, C. H. E., Villanueva, A. R., Kleerekoper, M., Frame, B., & Rao, D. S. (1983) · Journal of Clinical Investigation · doi:10.1172/jci111096 · Cited by 1,542
The epidemiology of corticosteroid-induced osteoporosis: a meta-analysis
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Alendronate for the Prevention and Treatment of Glucocorticoid-Induced Osteoporosis
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Self-citationMarkers of bone resorption predict hip fracture in elderly women: The EPIDOS prospective study
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Relationship of Early Changes in Bone Resorption to the Reduction in Fracture Risk With Risedronate
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Research works printed but not linked: 2
- Lane, 2005, β5 Integrin deficiency increases osteoclast generation and activity and induces greater bone loss following ovariectomy, J Bone Miner Res, 20, 58
- Parfitt, 1983, Relationships between surface, area, and thickness of iliac trabecular bone in aging and in osteoporosis, J Clin Invest, 72, 1396, 10.1172/JCI111096 · DOI
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Bonewald, L. F. (2011) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.320 · Cited by 2,287
Self-citationGlucocorticoid-induced osteoporosis: pathophysiology and therapy
CANALIS, E. M., Mazziotti, G., Giustina, A., & Bilezikian, J. P. (2007) · Osteoporosis International · doi:10.1007/s00198-007-0394-0 · Cited by 1,131
Osteocytes, mechanosensing and Wnt signaling
Bonewald, L. F., & Johnson, M. L. (2008) · Bone · doi:10.1016/j.bone.2007.12.224 · Cited by 1,076
The Osteocyte: An Endocrine Cell … and More
Dallas, S. L., Prideaux, M., & Bonewald, L. F. (2013) · Endocrine Reviews · doi:10.1210/er.2012-1026 · Cited by 1,042
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Metadata
- Authors
- Lane, N. E., Yao, W., Balooch, M., Nalla, R. K., Balooch, G., Habelitz, S., Kinney, J. H., & Bonewald, L. F.
- Affiliations
- University of California, Davis (US)
- Lawrence Berkeley National Laboratory (US)
- University of California, San Francisco (US)
- University of Missouri–Kansas City (US)
- Corresponding author
- Lane, Nancy E
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-03-01
- Volume
- 21
- Issue
- 3
- Pages
- 466-476
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- PMC
- PMC1797152
- OpenAlex
- W2074016952
- Cited by
- 355
- References
- 47
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 11 · 2023: 13 · 2022: 16 · 2021: 17 · 2020: 22 · 2019: 21 · 2018: 20 · 2017: 16 · 2016: 15 · 2015: 26 · 2014: 13 · 2013: 17 · 2012: 21
- FWCI
- 23.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (5r01dk046661-05)
- University of California, San Francisco
- National Cancer Institute (P50CA095817)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK46661-10)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR043052, K24AR048841, 1-K24-AR-48841-02, P01AR046798, P01AR46798)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD046661)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Keywords
- glucocorticoid-induced osteoporosis, prednisolone, trabecular bone microarchitecture, bone material properties, elastic modulus, ovariectomy, estrogen deficiency, bone fragility, fracture risk, bone strength, lumbar vertebrae
- MeSH terms
- Animals, Bone and Bones, Elasticity, Estrogens, Glucocorticoids, Male, Mice, Inbred Strains, Osteocytes, Placebos, Prednisolone, Tomography, X-Ray Computed, Biomarkers, Bone Density, Bone Remodeling, Compressive Strength, Mice
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