Age-related alterations in the diffusional transport of amino acids across the human Bruch’s-choroid complex
Abstract
Photoreceptor maintenance is dependent on effective delivery of nutrients from the choroidal circulation by way of the acellular Bruch's membrane and the retinal pigment epithelium. Aging of Bruch's membrane is associated with thickening, increased cross linking of fibers, and deposition of debris culminating in reduced porosity. The present study has investigated the effects of aging on the diffusional transport of eight amino acids across Bruch's membrane in 19 human donors. Diffusion studies were carried out in Ussing chambers, and the amount of time-dependent transfer of amino acids across the preparation was quantified by reverse-phase high-performance liquid chromatography. Diffusion rates for all amino acids showed a significant linear decline with aging of donor. The importance of this reduction in delivery of amino acids is discussed with reference to both normal physiology and age-related macular degeneration.
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BrM thickening with age is likely due to the accumulation of lipids, including esterified cholesterol, fatty acids, and triglycerides, which reduces its permeability.
“This phenomenon is likely due to the accumulation of lipids, including esterified cholesterol, fatty acids, and triglycerides [48,49], which reduces its permeability [50,51].”
From Bruch’s Membrane: A Key Consideration with Complement-Based Therapies for Age-Related Macular Degeneration (2023) · cites this paper as [51]
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- Authors
- Hussain, A. A., Rowe, L., & Marshall, J.
- Affiliations
- King's College London (GB)
- Venue
- Journal of the Optical Society of America A
- Publisher
- Optica Publishing Group
- Published
- 2002-01-01
- Volume
- 19
- Issue
- 1
- Pages
- 166
- ISSN
- 1084-7529 (ISSN-L)
- 1520-8532
- OpenAlex
- W1991870061
- Cited by
- 77
- References
- 46
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 5 · 2022: 2 · 2020: 2 · 2019: 3 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 5
- FWCI
- 5.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the Optical Society of America A (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Research Portal (King's College London) (submittedVersion)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- Bruch's membrane, aging, choroid, retinal pigment epithelium, age-related macular degeneration, nutrient transport, membrane porosity
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Aging, Amino Acids, Biological Transport, Choroid, Chromatography, High Pressure Liquid, Diffusion, Humans, Middle Aged, Bruch Membrane
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