Cited by 77Retinal Diseases and TreatmentsRetinal Development and DisordersBarrier Structure and Function Studies

Age-related alterations in the diffusional transport of amino acids across the human Bruch’s-choroid complex

Hussain, Ali A., Rowe, Lisa, Marshall, John·2002·Journal of the Optical Society of America A

Abstract

Photoreceptor maintenance is dependent on effective delivery of nutrients from the choroidal circulation by way of the acellular Bruch's membrane and the retinal pigment epithelium. Aging of Bruch's membrane is associated with thickening, increased cross linking of fibers, and deposition of debris culminating in reduced porosity. The present study has investigated the effects of aging on the diffusional transport of eight amino acids across Bruch's membrane in 19 human donors. Diffusion studies were carried out in Ussing chambers, and the amount of time-dependent transfer of amino acids across the preparation was quantified by reverse-phase high-performance liquid chromatography. Diffusion rates for all amino acids showed a significant linear decline with aging of donor. The importance of this reduction in delivery of amino acids is discussed with reference to both normal physiology and age-related macular degeneration.

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Registry

Crossref · 10.1364/josaa.19.000166

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Authors
Hussain, A. A., Rowe, L., & Marshall, J.
Affiliations
King's College London (GB)
Venue
Journal of the Optical Society of America A
Published
2002-01-01
Volume
19
Issue
1
Pages
166
ISSN
  • 1084-7529 (ISSN-L)
  • 1520-8532
OpenAlex
W1991870061
Cited by
77
References
46
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 5 · 2022: 2 · 2020: 2 · 2019: 3 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 5
FWCI
5.82
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of the Optical Society of America A (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
  • Research Portal (King's College London) (submittedVersion)
Topics
  • Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Keywords
Bruch's membrane, aging, choroid, retinal pigment epithelium, age-related macular degeneration, nutrient transport, membrane porosity
MeSH terms
Adolescent, Adult, Aged, Aged, 80 and over, Aging, Amino Acids, Biological Transport, Choroid, Chromatography, High Pressure Liquid, Diffusion, Humans, Middle Aged, Bruch Membrane

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