Cited by 416Genomics and Phylogenetic StudiesGenetic and phenotypic traits in livestockChromosomal and Genetic Variations

Multi-platform next-generation sequencing of the domestic turkey (Meleagris gallopavo): genome assembly and analysis

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Dalloul, Rami A., Long, Julie A., Zimin, Aleksey V., Aslam, Luqman, Beal, Kathryn, Ann Blomberg, Le, Bouffard, Pascal, Burt, David W. +63 more·2010·PLoS Biology

Abstract

A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided a cost-effective assembly of the genome sequence of the domestic turkey (Meleagris gallopavo). Heterozygosity of the sequenced source genome allowed discovery of more than 600,000 high quality single nucleotide variants. Despite this heterozygosity, the current genome assembly (∼1.1 Gb) includes 917 Mb of sequence assigned to specific turkey chromosomes. Annotation identified nearly 16,000 genes, with 15,093 recognized as protein coding and 611 as non-coding RNA genes. Comparative analysis of the turkey, chicken, and zebra finch genomes, and comparing avian to mammalian species, supports the characteristic stability of avian genomes and identifies genes unique to the avian lineage. Clear differences are seen in number and variety of genes of the avian immune system where expansions and novel genes are less frequent than examples of gene loss. The turkey genome sequence provides resources to further understand the evolution of vertebrate genomes and genetic variation underlying economically important quantitative traits in poultry. This integrated approach may be a model for providing both gene and chromosome level assemblies of other species with agricultural, ecological, and evolutionary interest.

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  • The HGFv1 assembly is compatible with current poultry reference genomes.

    “The HGFv1 assembly is compatible with current poultry reference genomes (Dalloul et al. 2010; Shapiro et al. 2013; Lu et al. 2015; ZhOu et al. 2018; Morris et al. 2020).”

    From Genomic Analyses Unveil Helmeted Guinea Fowl (<i>Numida meleagris</i>) Domestication in West Africa (2021) · cites this paper as Dalloul et al. 2010

    The Introduction identifies the paper’s draft turkey genome as a domestic avian genome, supporting its use as an example of a poultry reference genome. The Methods describe alignment with chicken and zebra finch genomes, but the paper does not mention HGFv1 or establish that HGFv1 is compatible with those genomes. (LLM, conf=0.85)

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Authors
Dalloul, R. A., Long, J. A., Zimin, A. V., Aslam, L., Beal, K., Ann Blomberg, L., Bouffard, P., Burt, D. W., Crasta, O., Crooijmans, R. P. M. A., Cooper, K., Coulombe, R. A., De, S., Delany, M. E., Dodgson, J. B., Dong, J. J., Evans, C., Frederickson, K. M., Flicek, P., Florea, L., Folkerts, O., Groenen, M. A. M., Harkins, T. T., Herrero, J., Hoffmann, S., Megens, H.-J., Jiang, A., de Jong, P., Kaiser, P., Kim, H., Kim, K.-W., Kim, S., Langenberger, D., Lee, M.-K., Lee, T., Mane, S., Marcais, G., Marz, M., McElroy, A. P., Modise, T., Nefedov, M., Notredame, C., Paton, I. R., Payne, W. S., Pertea, G., Prickett, D., Puiu, D., Qioa, D., Raineri, E., Ruffier, M., Salzberg, S. L., Schatz, M. C., Scheuring, C., Schmidt, C. J., Schroeder, S., Searle, S. M. J., Smith, E. J., Smith, J., Sonstegard, T. S., Stadler, P. F., Tafer, H., Tu, Z. (., Van Tassell, C. P., Vilella, A. J., Williams, K. P., Yorke, J. A., Zhang, L., Zhang, H.-B., Zhang, X., Zhang, Y., & Reed, K. M.
Affiliations
  • Virginia Tech (US)
  • Agricultural Research Service (US)
  • University of Maryland, College Park (US)
  • European Bioinformatics Institute (GB)
  • Roslin Institute (GB)
  • Wageningen University & Research (NL)
  • Utah State University (US)
  • National Institutes of Health (US)
  • University of California, Davis (US)
  • Michigan State University (US)
  • Texas A&M University (US)
  • Leipzig University (DE)
  • The Pirbright Institute (GB)
  • Seoul National University (KR)
  • Philipps University of Marburg (DE)
  • Universitat Pompeu Fabra (ES)
  • Wellcome Sanger Institute (GB)
  • University of Delaware (US)
  • University of Vienna (AT)
  • University of Minnesota (US)
Corresponding author
Reed, Kent M.
Venue
PLoS Biology
Published
2010-09-07
Volume
8
Issue
9
Pages
e1000475
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
OpenAlex
W2158682869
Cited by
416
References
104
Citations by year
2026: 4 · 2025: 11 · 2024: 11 · 2023: 11 · 2022: 11 · 2021: 14 · 2020: 28 · 2019: 20 · 2018: 25 · 2017: 18 · 2016: 21 · 2015: 38 · 2014: 50 · 2013: 51 · 2012: 40
FWCI
20.08
Type
journal article
Language
en
Open access
Locations
  • PLoS Biology (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • UCL Discovery (University College London) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
  • Publikationsdatenbank der Fraunhofer-Gesellschaft (Fraunhofer-Gesellschaft) (submittedVersion, other-oa)
  • OakTrust (Texas A&M University Libraries) (submittedVersion)
  • Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, public-domain)
  • VTechWorks (Virginia Tech) (submittedVersion, cc-by-nc-nd)
  • VTechWorks (Virginia Tech) (submittedVersion, public-domain)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Funders
  • National Science Foundation (0616585)
  • U.S. Department of Agriculture (2008-04049)
  • Utah State University
  • University of Minnesota
  • Wellcome Trust
  • European Commission
  • Freistaat Sachsen
  • National Institutes of Health
  • National Institute of Food and Agriculture (NRSP-8, 2008-04049)
  • Agricultural Research Service
  • Biotechnology and Biological Sciences Research Council (BB/D013704/2, BB/D013704/1)
  • National Institute on Aging
  • National Human Genome Research Institute (R01HG002945, F33HG006641)
  • National Institute of General Medical Sciences (R25GM072767, R01GM083873, R25GM066534)
  • U.S. National Library of Medicine (R01LM006845, LM006845)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
domestic turkey, Meleagris gallopavo, genome assembly, single nucleotide variants, heterozygosity, avian genome evolution, avian immune system, gene loss, comparative genomics, chickens, zebra finch, poultry genetics, chromosome-level genome assembly
MeSH terms
Animals, Base Sequence, Chromosome Mapping, DNA, Sequence Homology, Nucleic Acid, Species Specificity, Turkeys, Genome, Sequence Analysis, DNA, Polymorphism, Single Nucleotide

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