The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Activation of P2X7 Receptor by ATP Plays an Important Role in Regulating Inflammatory Responses during Acute Viral Infection
Open accessAbstract
Acute viral infection causes damages to the host due to uncontrolled viral replication but even replication deficient viral vectors can induce systemic inflammatory responses. Indeed, overactive host innate immune responses to viral vectors have led to devastating consequences. Macrophages are important innate immune cells that recognize viruses and induce inflammatory responses at the early stage of infection. However, tissue resident macrophages are not easily activated by the mere presence of virus suggesting that their activation requires additional signals from other cells in the tissue in order to trigger inflammatory responses. Previously, we have shown that the cross-talk between epithelial cells and macrophages generates synergistic inflammatory responses during adenoviral vector infection. Here, we investigated whether ATP is involved in the activation of macrophages to induce inflammatory responses during an acute adenoviral infection. Using a macrophage-epithelial cell co-culture system we demonstrated that ATP signaling through P2X(7) receptor (P2X(7)R) is required for induction of inflammatory mediators. We also showed that ATP-P2X(7)R signaling regulates inflammasome activation as inhibition or deficiency of P2X(7)R as well as caspase-1 significantly reduced IL-1β secretion. Furthermore, we found that intranasal administration of replication deficient adenoviral vectors in mice caused a high mortality in wild-type mice with symptoms of acute respiratory distress syndrome but the mice deficient in P2X(7)R or caspase-1 showed increased survival. In addition, wild-type mice treated with apyrase or inhibitors of P2X(7)R or caspase-1 showed higher rates of survival. The improved survival in the P2X(7)R deficient mice correlated with diminished levels of IL-1β and IL-6 and reduced neutrophil infiltration in the early phase of infection. These results indicate that ATP, released during viral infection, is an important inflammatory regulator that activates the inflammasome pathway and regulates inflammatory responses.
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“Moreover, P2X7 receptor activation exacerbated inflammation, enhanced tissue damage, increased mortality, and worsened lung pathology involving P2X7 receptor expression or activation, such as observed in several other studies with e.g., influenza A virus [110] and adenovirus [111].”
From Hyperactivation of P2X7 receptors as a culprit of COVID-19 neuropathology (2021) · cites this paper as [111]
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Differential activation of the inflammasome by caspase-1 adaptors ASC and Ipaf
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The AIM2 inflammasome is essential for host defense against cytosolic bacteria and DNA viruses
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The P2X7 Receptor: A Key Player in IL-1 Processing and Release
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Meyer, L. (2017) · theses.fr (ABES) · doi:10.70675/4cc74c2fzcc62z4f43za393zd727cf5f3bee
Kargarpour, Z., Aschenbrenner, B., Kramer, M., Metekol, S., Begunic, A., Geleff, S., Obermayr, E., Zeillinger, R., Papaporfyriou, A., Renner, A., et al. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1940896
Metadata
- Authors
- Lee, B. H., Hwang, D. M., Palaniyar, N., Grinstein, S., Philpott, D. J., & Hu, J.
- Affiliations
- University of Toronto (CA)
- University Health Network (CA)
- Corresponding author
- Hu, Jim
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-04-25
- Volume
- 7
- Issue
- 4
- Pages
- e35812
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3338466
- OpenAlex
- W2072521717
- Cited by
- 102
- References
- 48
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 11 · 2020: 7 · 2019: 7 · 2018: 8 · 2017: 15 · 2016: 9 · 2015: 5 · 2014: 7 · 2013: 6 · 2012: 2
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- Cystic Fibrosis Canada
- Canadian Institutes of Health Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- P2X7 receptor, ATP signaling, acute viral infection, adenoviral vector, macrophage activation, inflammasome, caspase-1, IL-1β, interleukin-6, neutrophil infiltration, acute respiratory distress syndrome, innate immunity
- MeSH terms
- Acute Disease, Adenosine Triphosphate, Adenoviridae, Adenoviridae Infections, Animals, Cell Communication, Enzyme Inhibitors, Epithelial Cells, Immunity, Innate, Inflammation, Macrophages, Signal Transduction, Interleukin-6, Mice, Knockout, Coculture Techniques, Caspase 1, Neutrophil Infiltration, Mice, Interleukin-1beta, Receptors, Purinergic P2X7, Inflammasomes, Caspase Inhibitors
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