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Changes in MicroRNA Expression in the Whole Hippocampus and Hippocampal Synaptoneurosome Fraction following Pilocarpine Induced Status Epilepticus

Risbud, Rashmi M., Porter, Brenda E.·2013·PLoS ONE
DOI 10.1371/journal.pone.0053464PMC PMC3538591
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Figure 1 Total number of hippocampal microRNAs that are increased or decreased after SE at each time point. There are a large number of microRNAs that specifically decreased at 48 hours after SE. The specific microRNAs that change are listed on Tables 1, 2 and 3. We used a P value of 0.05 on a t-test to identify microRNAs that change.
Figure 1 Total number of hippocampal microRNAs that are increased or decreased after SE at each time point. There are a large number of microRNAs that specifically decreased at 48 hours after SE. The specific microRNAs that change are listed on Tables 1, 2 and 3. We used a P value of 0.05 on a t-test to identify microRNAs that change.CC BY · PubMed Central
Figure 2 Real time PCR confirmed changes in 6 of 8 microRNAs identified as either decreased or increased on the hippocampal microRNA arrays following SE. P*<0.05 T-test, control N = 8 to 9 and SE N = 7 to 9 samples per group.
Figure 2 Real time PCR confirmed changes in 6 of 8 microRNAs identified as either decreased or increased on the hippocampal microRNA arrays following SE. P*<0.05 T-test, control N = 8 to 9 and SE N = 7 to 9 samples per group.CC BY · PubMed Central
Figure 3 There is a decrease in the ratio of synaptoneurosome/nuclear microRNA fractions in the hippocampus following SE. A) A dot plot of the synaptoneurosome/nuclear ratio of all of the microRNAs expressed above background in at least four arrays from either nuclear or synaptoneurosome fractions. MicroRNAs in the hippocampus of control animals and 48 hours after SE are plotted with the mean and standard error shown by black lines. B) The synaptoneurosome/nuclear ratio of all of the microRNAs expressed above background are plotted left to right, based on the microRNAs that are most highly enriched to the least in the synaptoneurosome fraction in the control hippocampus. X represents the synaptoneurosome/nuclear microRNA ratio of the control samples, and directly below each X the 0 is the same microRNAs synaptoneurosome/nuclear ratio of the SE samples. Specific microRNAs and their synaptoneurosome/nuclear microRNA ratio are shown in Table 4. We have highlighted in red a subset of microRNAs that have previously been shown to be enriched in dendrites or synaptoneurosomes and highlighted in green a subset of microRNAs that have previously been shown to be expressed at low levels in dendrites or synaptoneurosomes [10], [11], [31].
Figure 3 There is a decrease in the ratio of synaptoneurosome/nuclear microRNA fractions in the hippocampus following SE. A) A dot plot of the synaptoneurosome/nuclear ratio of all of the microRNAs expressed above background in at least four arrays from either nuclear or synaptoneurosome fractions. MicroRNAs in the hippocampus of control animals and 48 hours after SE are plotted with the mean and standard error shown by black lines. B) The synaptoneurosome/nuclear ratio of all of the microRNAs expressed above background are plotted left to right, based on the microRNAs that are most highly enriched to the least in the synaptoneurosome fraction in the control hippocampus. X represents the synaptoneurosome/nuclear microRNA ratio of the control samples, and directly below each X the 0 is the same microRNAs synaptoneurosome/nuclear ratio of the SE samples. Specific microRNAs and their synaptoneurosome/nuclear microRNA ratio are shown in Table 4. We have highlighted in red a subset of microRNAs that have previously been shown to be enriched in dendrites or synaptoneurosomes and highlighted in green a subset of microRNAs that have previously been shown to be expressed at low levels in dendrites or synaptoneurosomes [10], [11], [31].CC BY · PubMed Central
Figure 4 Real-time PCR demonstrated that HDAC3 and GAPDH mRNAs are enriched in the nuclear compared to synaptoneurosome fractions of both control and SE treated animal’s hippocampi. N = 6 for all samples, Nuclear control (nuc-ctl), synaptoneurosome control (syn-ctl), Nuclear SE (nuc-sz), synaptoneurosome SE (syn-sz), P**<0.01 Mann-Whitney.
Figure 4 Real-time PCR demonstrated that HDAC3 and GAPDH mRNAs are enriched in the nuclear compared to synaptoneurosome fractions of both control and SE treated animal’s hippocampi. N = 6 for all samples, Nuclear control (nuc-ctl), synaptoneurosome control (syn-ctl), Nuclear SE (nuc-sz), synaptoneurosome SE (syn-sz), P**<0.01 Mann-Whitney.CC BY · PubMed Central
Figure 5 Real-time PCR demonstrated that MTAP3 mRNA is enriched in the synaptoneurosome compared to nuclear fractions of control animals hippocampus, Mann-Whitney P**<0.01. MTAP3 mRNA levels are similar in synaptoneurosome and nuclear fractions 48 hours after SE. CAMK2α, GRIA2 and DLGAP2 mRNA as measured by real-time PCR was similar in the synaptoneurosome and nuclear fractions of both control and SE animal’s hippocampi. Following SE CAMK2α and DLGAP2 mRNA decreased in both the nuclear and synaptoneurosome fractions, P * 0.05, P** 0.01, P*** 0.001 ANOVA with Bonferroni corrections N = 6 for all samples, nuclear control (nuc-ctl), synaptoneurosome control (syn-ctl), nuclear SE (nuc-sz), synaptoneurosome SE (syn-sz).
Figure 5 Real-time PCR demonstrated that MTAP3 mRNA is enriched in the synaptoneurosome compared to nuclear fractions of control animals hippocampus, Mann-Whitney P**<0.01. MTAP3 mRNA levels are similar in synaptoneurosome and nuclear fractions 48 hours after SE. CAMK2α, GRIA2 and DLGAP2 mRNA as measured by real-time PCR was similar in the synaptoneurosome and nuclear fractions of both control and SE animal’s hippocampi. Following SE CAMK2α and DLGAP2 mRNA decreased in both the nuclear and synaptoneurosome fractions, P * 0.05, P** 0.01, P*** 0.001 ANOVA with Bonferroni corrections N = 6 for all samples, nuclear control (nuc-ctl), synaptoneurosome control (syn-ctl), nuclear SE (nuc-sz), synaptoneurosome SE (syn-sz).CC BY · PubMed Central

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Metadata

Authors
Risbud, R. M., & Porter, B. E.
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-01-07
Volume
8
Issue
1
Pages
e53464
ISSN
1932-6203
DOI
10.1371/journal.pone.0053464
PMC
PMC3538591
Cited by
81
References
3
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (unspecified)

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