Conditional and specific cell ablation in the marine annelid Platynereis dumerilii
Open accessAbstract
The marine annelid Platynereis dumerilii has become a model system for evo-devo, neurobiology and marine biology. The functional assessment of its cell types, however, has so far been very limited. Here we report on the establishment of a generally applicable, cell type specific ablation technique to overcome this restriction. Using a transgenic strain expressing the bacterial enzyme nitroreductase (ntr) under the control of the worm's r-opsin1 locus, we show that the demarcated photoreceptor cells can be specifically ablated by the addition of the prodrug metronidazole (mtz). TUNEL staining indicates that ntr expressing cells undergo apoptotic cell death. As we used a transgenic strain co-expressing ntr with enhanced green fluorescent protein (egfp) coding sequence, we were able to validate the ablation of photoreceptors not only in fixed tissue, using r-opsin1 riboprobes, but also by monitoring eGFP+ cells in live animals. The specificity of the ablation was demonstrated by the normal presence of the eye pigment cells, as well as of neuronal markers expressed in other cells of the brain, such as phc2, tyrosine hydroxylase and brn1/2/4. Additional analyses of the position of DAPI stained nuclei, the brain's overall neuronal scaffold, as well as the positions and projections of serotonergic neurons further confirmed that mtz treatment did not induce general abnormalities in the worm's brain. As the prodrug is administered by adding it to the water, targeted ablation of specific cell types can be achieved throughout the life of the animal. We show that ablation conditions need to be adjusted to the size of the worms, likely due to differences in the penetration of the prodrug, and establish ablation conditions for worms containing 10 to 55 segments. Our results establish mtz/ntr mediated conditional cell ablation as a powerful functional tool in Platynereis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The marine bristleworm Platynereis dumerilii is well suited to tackle the endocrine basis of this integration.
“The marine bristleworm Platynereis dumerilii is well suited to tackle the endocrine basis of this integration.”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [10]
Platynereis is molecularly accessible, and various genetic toolkits allow functional studies.
“Moreover, this species is molecularly accessible, and various genetic toolkits allow functional studies (9–11).”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [10]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The Tol2kit: A multisite gateway‐based construction kit for Tol2 transposon transgenesis constructs
Kwan, K. M., Fujimoto, E., Grabher, C., Mangum, B. D., Hardy, M., Campbell, D. S., Parant, J. M., Yost, H. J., Kanki, J. P., & Chien, C.-B. (2007) · Developmental Dynamics · doi:10.1002/dvdy.21343 · Cited by 1,778 · see citing works
Conditional targeted cell ablation in zebrafish: A new tool for regeneration studies
Curado, S., Anderson, R. M., Jungblut, B., Mumm, J. S., Schroeter, E. H., & Stainier, D. Y. R. (2007) · Developmental Dynamics · doi:10.1002/dvdy.21100 · Cited by 509 · see citing works
Denes, A. S., Jékely, G., Steinmetz, P. R. H., Raible, F., Snyman, H., Prud'homme, B., Ferrier, D. E. K., Balavoine, G., & Arendt, D. (2007) · Cell · doi:10.1016/j.cell.2007.02.040 · Cited by 480 · see citing works
Self-citationCiliary photoreceptors with a vertebrate-type opsin in an invertebrate brain
Arendt, D., Tessmar-Raible, K., Snyman, H., Dorresteijn, A. W., & Wittbrodt, J. (2004) · Science · doi:10.1126/science.1099955 · Cited by 458 · see citing works
Self-citationCurado, S., Stainier, D. Y. R., & Anderson, R. M. (2008) · Nature Protocols · doi:10.1038/nprot.2008.58 · Cited by 440 · see citing works
Tessmar-Raible, K., Raible, F., Christodoulou, F., Guy, K., Rembold, M., Hausen, H., & Arendt, D. (2007) · Cell · doi:10.1016/j.cell.2007.04.041 · Cited by 384 · see citing works
Self-citationTomer, R., Denes, A. S., Tessmar-Raible, K., & Arendt, D. (2010) · Cell · doi:10.1016/j.cell.2010.07.043 · Cited by 317 · see citing works
Self-citationMechanism of phototaxis in marine zooplankton
Jékely, G., Colombelli, J., Hausen, H., Guy, K., Stelzer, E., Nédélec, F., & Arendt, D. (2008) · Nature · doi:10.1038/nature07590 · Cited by 298 · see citing works
Deep Brain Photoreceptors Control Light-Seeking Behavior in Zebrafish Larvae
Fernandes, A. M., Fero, K. C., Arrenberg, A. B., Bergeron, S. A., Driever, W., & Burgess, H. A. (2012) · Current Biology · doi:10.1016/j.cub.2012.08.016 · Cited by 274 · see citing works
Vertebrate-Type Intron-Rich Genes in the Marine Annelid <i>Platynereis dumerilii</i>
Raible, F., Tessmar-Raible, K., Osoegawa, K., Wincker, P., Jubin, C., Balavoine, G., Ferrier, D., Benes, V., de Jong, P., Weissenbach, J., et al. (2005) · Science · doi:10.1126/science.1119089 · Cited by 257 · see citing works
Self-citationThe normal development of Platynereis dumerilii (Nereididae, Annelida)
Fischer, A., Henrich, T., & Arendt, D. (2010) · Frontiers in Zoology · doi:10.1186/1742-9994-7-31 · Cited by 235 · see citing works
Arendt, D., Tessmar, K., de Campos-Baptista, M.-I. M., Dorresteijn, A., & Wittbrodt, J. (2002) · Development · doi:10.1242/dev.129.5.1143 · Cited by 192 · see citing works
Tang, W., Ehrlich, I., Wolff, S. B. E., Michalski, A., Wölfl, S., Hasan, M. T., Lüthi, A., & Sprengel, R. (2009) · Journal of Neuroscience · doi:10.1523/jneurosci.0359-09.2009 · Cited by 179 · see citing works
A toolkit for high-throughput, cross-species gene engineering in Drosophila
Ejsmont, R. K., Sarov, M., Winkler, S., et al. (2009) · Nature Methods · doi:10.1038/nmeth.1334 · Cited by 111 · see citing works
Hedgehog Signaling Regulates Segment Formation in the Annelid Platynereis
Dray, N., Tessmar‐Raible, K., Gouar, M. L., Vibert, L., Christodoulou, F., Schipany, K., Guillou, A., Zantke, J., Snyman, H., Béhague, J., et al. (2010) · Science · doi:10.1126/science.1188913 · Cited by 98 · see citing works
Self-citationBackfisch, B., Veedin Rajan, V. B., Fischer, R. M., Lohs, C., Arboleda, E., Tessmar-Raible, K., & Raible, F. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1209657109 · Cited by 93 · see citing works
Self-citationA transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis
White, Y. A. R., Woods, D. C., & Wood, A. W. (2011) · Developmental Dynamics · doi:10.1002/dvdy.22695 · Cited by 33 · see citing works
Raible, F., & Tessmar‐Raible, K. (2014) · Current Biology · doi:10.1016/j.cub.2014.06.032 · Cited by 21 · see citing works
Self-citationMetronidazole Induced DNA Damage in Somatic Cells of Drosophila melanogaster
Palermo, A. M., & Mudry, M. D. (2013) · Current Drug Safety · doi:10.2174/15748863113089990035 · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Andreatta, G., Broyart, C., Borghgraef, C., Vadiwala, K., Kozin, V., Polo, A., Bileck, A., Beets, I., Schoofs, L., Gerner, C., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1910262116 · Cited by 61 · see citing works
Self-citationBannister, S., Antonova, O., Polo, A., Lohs, C., Hallay, N., Valinciute, A., Raible, F., & Tessmar-Raible, K. (2014) · Genetics · doi:10.1534/genetics.113.161091 · Cited by 56 · see citing works
“This approach has already contributed to the discovery of r-opsin1+ peripheral photoreceptor cells (Backfisch et al. 2013).”
Background
- Authors
- Veedin-Rajan, V. B., Fischer, R. M., Raible, F., & Tessmar-Raible, K.
- Affiliations
- University of Vienna (AT)
- ORCID
- Raible, Florian
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-09-24
- Volume
- 8
- Issue
- 9
- Pages
- e75811
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3782428
- OpenAlex
- W2088487900
- Cited by
- 15
- References
- 19
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 1 · 2019: 2 · 2018: 2 · 2015: 1 · 2014: 6
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Phaidra (Universität Wien) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Austrian Science Fund (#AY0041321)
- Universität Wien
- FP7 Ideas: European Research Council (260304)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Keywords
- Platynereis dumerilii, nitroreductase, metronidazole, photoreceptors, apoptosis, transgenic worms, brain neurons, serotonergic neurons, prodrugs, live imaging, enhanced green fluorescent protein
- MeSH terms
- Animals, Metronidazole, Neurons, Nitroreductases, Photoreceptor Cells, Polychaeta, Prodrugs, Cell Death, Apoptosis, Animals, Genetically Modified, Green Fluorescent Proteins, Ablation Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
